| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,015,130 | A→G | 40.3% | G313G (GGT→GGC) | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,015,130 | 0 | A | G | 40.3% | 42.7 / 81.4 | 77 | G313G (GGT→GGC) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base A (26/20); new base G (10/21); total (36/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.17e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.90e-01 | |||||||||||
CGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCA > Exported/1015063‑1015199 | cGATCCAGATACCTTCGGGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCTGGAGACGCCCATgcc < 1:959164/70‑1 (MQ=255)cGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATgcc > 1:631225/1‑70 (MQ=255) gATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATgccga < 1:2196846/71‑1 (MQ=255) ccAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATgccgaccg > 1:2889007/1‑71 (MQ=255) ccccTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAg > 1:2236504/1‑70 (MQ=255) ccccTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCACATACCGACCGGGCAg > 1:2294138/1‑70 (MQ=255) cccTCACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGa < 1:3037613/71‑1 (MQ=255) cTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGCCCGGGCAGGAt > 1:5521129/1‑70 (MQ=255) cTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATg > 1:4567470/1‑71 (MQ=255) cTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATg > 1:3316979/1‑71 (MQ=255) tGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATgc > 1:2567914/1‑71 (MQ=255) gcGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATgcgc > 1:678811/2‑71 (MQ=255) gcGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATgc < 1:3486527/68‑1 (MQ=255) ggTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCg < 1:2419461/71‑1 (MQ=255) gTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGt < 1:1919808/71‑1 (MQ=255) ttGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGt > 1:938789/1‑70 (MQ=255) ttGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGt > 1:4734037/1‑70 (MQ=255) tGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGa > 1:2493085/1‑71 (MQ=255) tGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGa > 1:4244530/1‑71 (MQ=255) tGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGa > 1:1126322/1‑71 (MQ=255) gATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGAc > 1:5690256/1‑71 (MQ=255) gATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGAc > 1:5197910/1‑71 (MQ=255) aTCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATaccgaccg > 1:4283364/1‑52 (MQ=255) ttttCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGa < 1:1891375/56‑1 (MQ=255) ttttCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGt < 1:519775/69‑1 (MQ=255) cGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGt < 1:2708153/67‑1 (MQ=255) gCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGAc > 1:1503710/1‑64 (MQ=255) gCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCa < 1:820874/70‑1 (MQ=255) gATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGa < 1:5118496/70‑1 (MQ=255) atatTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAAt > 1:5249545/1‑71 (MQ=255) atTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCa > 1:4396330/1‑71 (MQ=255) ttACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAAc > 1:4556881/1‑71 (MQ=255) aCGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGAt > 1:2811572/1‑43 (MQ=255) aCGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGa > 1:2761736/1‑52 (MQ=255) aCGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGa > 1:146559/1‑52 (MQ=255) aCGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGa > 1:3645640/1‑52 (MQ=255) aCGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCAcgc > 1:4825718/1‑71 (MQ=255) cGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAAcgcg < 1:4992893/71‑1 (MQ=255) ggTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCAcgc < 1:124909/69‑1 (MQ=255) ggTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCgg < 1:5372228/71‑1 (MQ=255) agcggagcagGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGt > 1:5579161/1‑71 (MQ=255) agcagagcagGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATg < 1:4931657/70‑1 (MQ=255) cggagcagGAGACGCCCATGCCGACCGGGCAGGATGCGCCGt < 1:4277022/40‑1 (MQ=38) cggagcagGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGa < 1:2728780/42‑1 (MQ=38) cagagcagGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCAcgcg < 1:746093/64‑1 (MQ=255) cagagcagGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCg < 1:3972203/71‑1 (MQ=255) ggagcagGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCg < 1:4468816/69‑1 (MQ=255) ggagcagGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGt < 1:1066112/70‑1 (MQ=255) agagcagGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCg < 1:1080131/70‑1 (MQ=255) agagcagGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCg < 1:4041384/70‑1 (MQ=255) agcagGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAAcgcg < 1:2555907/61‑1 (MQ=255) cagGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGc < 1:44878/71‑1 (MQ=255) agGAGACGCCCATGCCGACCGGGCAGGATGCGCCGt > 1:2062798/1‑36 (MQ=255) agGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGcgcg < 1:1667475/71‑1 (MQ=255) ggAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGcgcg > 1:5622372/1‑70 (MQ=255) ggAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGa < 1:4194267/71‑1 (MQ=255) ggAGACGCCCATACCGACCGGGCAGGATGCGCCGTGAc > 1:43725/1‑38 (MQ=255) ggAGACGCCCATACCGACCGGGCAGGATGCGCCGTGAc > 1:3562757/1‑38 (MQ=255) agaCGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAg < 1:1877428/71‑1 (MQ=255) agaCGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAg < 1:2178286/71‑1 (MQ=255) agaCGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAg < 1:550342/71‑1 (MQ=255) agaCGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAg < 1:3884826/71‑1 (MQ=255) gaCGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAg > 1:3118423/1‑70 (MQ=255) cGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTAt > 1:515647/1‑71 (MQ=255) gCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTAtt < 1:2853639/71‑1 (MQ=255) cccATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTgcgc < 1:2705980/62‑1 (MQ=255) cccATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTgcgc < 1:2241003/62‑1 (MQ=255) ccATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTa < 1:3622182/71‑1 (MQ=255) cATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTac > 1:3480242/1‑71 (MQ=255) cATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTac > 1:1605888/1‑71 (MQ=255) aTACCGACCGGGCAGTATGCGCCGTGACGTGGCAGGCGAAt < 1:4568092/41‑1 (MQ=255) aTACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAAt < 1:3047222/41‑1 (MQ=255) aTACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAAt < 1:1725854/41‑1 (MQ=255) aTACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAAt < 1:1732405/41‑1 (MQ=255) aTACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAAt < 1:4156888/41‑1 (MQ=255) tACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTa < 1:1456498/64‑1 (MQ=255) tACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTAttt < 1:2331947/67‑1 (MQ=255) tACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTAttt < 1:5133979/67‑1 (MQ=255) tACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTacca > 1:5632792/1‑71 (MQ=255) tACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTacca > 1:761644/1‑71 (MQ=255) tACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTacca > 1:3721887/1‑71 (MQ=255) tACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTacca > 1:4369366/1‑71 (MQ=255) | CGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCA > Exported/1015063‑1015199 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |