| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,015,163 | G→A | 43.9% | R302R (CGC→CGT) | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,015,163 | 0 | G | A | 43.9% | 50.4 / 131.6 | 114 | R302R (CGC→CGT) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base G (25/39); new base A (20/30); total (45/69) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.12e-01 | |||||||||||
Only 100 of 120 total aligned reads displayed.
GATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCG > Exported/1015097‑1015230 | gATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGa < 1:5118496/70‑1 (MQ=255) atatTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAAt > 1:5249545/1‑71 (MQ=255) atTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCa > 1:4396330/1‑71 (MQ=255) ttACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAAc > 1:4556881/1‑71 (MQ=255) aCGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCAcgc > 1:4825718/1‑71 (MQ=255) cGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAAcgcg < 1:4992893/71‑1 (MQ=255) ggTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCAcgc < 1:124909/69‑1 (MQ=255) ggTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCgg < 1:5372228/71‑1 (MQ=255) agcggagcagGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGt > 1:5579161/1‑71 (MQ=255) agcagagcagGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATg < 1:4931657/70‑1 (MQ=255) cagagcagGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCg < 1:3972203/71‑1 (MQ=255) ggagcagGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCg < 1:4468816/69‑1 (MQ=255) ggagcagGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGt < 1:1066112/70‑1 (MQ=255) agagcagGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCg < 1:1080131/70‑1 (MQ=255) agcagGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAAcgcg < 1:2555907/61‑1 (MQ=255) cagGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGc < 1:44878/71‑1 (MQ=255) agGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGcgcg < 1:1667475/71‑1 (MQ=255) ggAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGcgcg > 1:5622372/1‑70 (MQ=255) ggAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGa < 1:4194267/71‑1 (MQ=255) agaCGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAg < 1:3884826/71‑1 (MQ=255) agaCGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAg < 1:550342/71‑1 (MQ=255) agaCGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAg < 1:2178286/71‑1 (MQ=255) gaCGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAg > 1:3118423/1‑70 (MQ=255) cGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTAt > 1:515647/1‑71 (MQ=255) gCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTAtt < 1:2853639/71‑1 (MQ=255) cccATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTgcgc < 1:2705980/62‑1 (MQ=255) cccATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTgcgc < 1:2241003/62‑1 (MQ=255) ccATGCCGACCGGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTa < 1:3622182/71‑1 (MQ=255) cATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTac > 1:1605888/1‑71 (MQ=255) atgccgaccgGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTacc < 1:5183224/68‑1 (MQ=255) aTACCGACCGGGCAGTATGCGCCGTGACGTGGCAGGCGAAt < 1:4568092/41‑1 (MQ=255) aTACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAAt < 1:1732405/41‑1 (MQ=255) aTACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAAt < 1:4156888/41‑1 (MQ=255) aTACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAAt < 1:1725854/41‑1 (MQ=255) aTACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAAt < 1:3047222/41‑1 (MQ=255) tACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTa < 1:1456498/64‑1 (MQ=255) tACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTAttt < 1:5133979/67‑1 (MQ=255) tACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTAttt < 1:2331947/67‑1 (MQ=255) tACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTacca > 1:761644/1‑71 (MQ=255) tACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTacca > 1:5632792/1‑71 (MQ=255) tACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTacca > 1:3721887/1‑71 (MQ=255) ccgaccgGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTAtt > 1:3575939/1‑64 (MQ=255) ccgaccgGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTac < 1:5179/67‑1 (MQ=255) ccgaccgGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGt > 1:3366147/1‑49 (MQ=255) ccgaccgGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGt > 1:825820/1‑49 (MQ=255) ccgaccgGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGt > 1:4965512/1‑49 (MQ=255) ccgaccgGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTaccgc > 1:3481542/1‑68 (MQ=255) ccgaccgGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTaccgc > 1:426570/1‑68 (MQ=255) cgaccgGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTAccacca < 1:766822/71‑1 (MQ=255) cgaccgGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTAccacca < 1:3918109/71‑1 (MQ=255) cgaccgGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTAccgcca < 1:4751028/71‑1 (MQ=255) gaccgGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTacca > 1:3642065/1‑67 (MQ=255) gaccgGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTAccacca > 1:1116641/1‑70 (MQ=255) gaccgGGCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTAccgcca < 1:3716781/70‑1 (MQ=255) gggCAGGATGCGCCGTGACGTGGCAGACGGATAAcgcg < 1:807345/38‑1 (MQ=25) ggCAGGATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTacc > 1:5363327/1‑61 (MQ=255) cAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTAttt < 1:868233/56‑1 (MQ=255) cAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTgcg > 1:1080147/1‑70 (MQ=255) aGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCaa < 1:5593544/63‑1 (MQ=255) ggATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGt < 1:127455/41‑1 (MQ=255) ggATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGt < 1:496128/41‑1 (MQ=255) ggATGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTgcgcgc < 1:4035730/71‑3 (MQ=255) gATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCAcc > 1:3962549/1‑71 (MQ=255) gATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCAcc < 1:4296266/71‑1 (MQ=255) tGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTa > 1:1404639/1‑52 (MQ=255) tGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCAcc > 1:1704618/1‑69 (MQ=255) tGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTa > 1:3483944/1‑52 (MQ=255) tGCGCCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGcc > 1:3596755/1‑69 (MQ=255) cgcCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCaaa > 1:4584156/1‑58 (MQ=255) cgcCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAg < 1:3368764/70‑1 (MQ=255) ccGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTa < 1:3373173/44‑1 (MQ=255) ccGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGAcc < 1:2376671/71‑1 (MQ=255) gTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGAc > 1:4623788/1‑68 (MQ=255) gTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGt < 1:3361534/41‑1 (MQ=19) tGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTg < 1:2570077/56‑1 (MQ=255) tGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGAc < 1:502631/67‑1 (MQ=255) tGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAg > 1:4065708/1‑71 (MQ=255) tGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAg > 1:2578522/1‑71 (MQ=255) aCGTGGCAGGCGAATCACGCGGATGTCGTGAGCGaa > 1:2317324/1‑36 (MQ=255) aCGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAAc > 1:1413253/1‑52 (MQ=255) aCGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGAt > 1:2604271/1‑71 (MQ=255) aCGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAgtt < 1:5281815/71‑3 (MQ=255) aCGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAgtt < 1:4418975/71‑3 (MQ=255) aCGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAgt < 1:2940748/70‑2 (MQ=255) cGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCaa < 1:4456709/49‑1 (MQ=255) cGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCaa < 1:4795824/49‑1 (MQ=255) cGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCaa < 1:2038483/49‑1 (MQ=255) gTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGAttt < 1:1041444/71‑1 (MQ=255) gTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGAttt < 1:2163767/71‑1 (MQ=255) gTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTac > 1:5358333/1‑42 (MQ=19) gTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTa > 1:5089089/1‑41 (MQ=19) gTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTAccgcca < 1:2535727/47‑1 (MQ=255) tGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTAtt < 1:404026/38‑1 (MQ=255) tGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTAt < 1:653327/37‑1 (MQ=255) tGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGAtttt < 1:2860717/71‑1 (MQ=255) tGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGAtttt < 1:3543896/71‑1 (MQ=255) tGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGtttct < 1:2047092/71‑3 (MQ=255) gCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGAtttt < 1:5482134/65‑1 (MQ=255) gCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGAtt < 1:2112087/63‑1 (MQ=255) gCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGAtt < 1:2447383/63‑1 (MQ=255) | GATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCG > Exported/1015097‑1015230 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |