| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,015,214 | C→A | 27.1% | L285L (CTG→CTT) | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,015,214 | 0 | C | A | 27.1% | 184.0 / 51.7 | 92 | L285L (CTG→CTT) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base C (28/39); new base A (11/14); total (39/53) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 | |||||||||||
CGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCT > Exported/1015147‑1015284 | cgcCGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAg < 1:3368764/70‑1 (MQ=255) ccGTGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGAcc < 1:2376671/71‑1 (MQ=255) gTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGAc > 1:4623788/1‑68 (MQ=255) tGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGAc < 1:502631/67‑1 (MQ=255) tGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAg > 1:4065708/1‑71 (MQ=255) tGACGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAg > 1:2578522/1‑71 (MQ=255) aCGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGAt > 1:2604271/1‑71 (MQ=255) aCGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAgtt < 1:4418975/71‑3 (MQ=255) aCGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAgtt < 1:5281815/71‑3 (MQ=255) aCGTGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAgt < 1:2940748/70‑2 (MQ=255) gTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGAttt < 1:1041444/71‑1 (MQ=255) gTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGAttt < 1:2163767/71‑1 (MQ=255) tGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGAtttt < 1:3543896/71‑1 (MQ=255) tGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGAtttt < 1:2860717/71‑1 (MQ=255) tGGCAGACGGATAACGCGAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGtttct < 1:2047092/71‑3 (MQ=255) cAGGCGAATCACGGGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTgcg > 1:1171393/1‑71 (MQ=255) gCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGAtttt < 1:5482134/65‑1 (MQ=255) gCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGAtt < 1:2447383/63‑1 (MQ=255) gCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGAtt < 1:2112087/63‑1 (MQ=255) cGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGAtttt < 1:2560277/64‑1 (MQ=255) gAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCg < 1:1013409/71‑1 (MQ=255) gAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCg < 1:2654855/71‑1 (MQ=255) aaTCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAg > 1:5381085/1‑51 (MQ=255) aTCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGAt > 1:1184460/1‑71 (MQ=255) tCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGAt < 1:4131335/70‑1 (MQ=255) tCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATc < 1:3377907/71‑1 (MQ=255) tCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATc < 1:2457002/71‑1 (MQ=255) tCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATc < 1:2829265/71‑1 (MQ=255) aCGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAg > 1:187400/1‑71 (MQ=255) aCGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAg > 1:3904013/1‑71 (MQ=255) cgcgGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGc < 1:3186520/71‑1 (MQ=255) cgcgAATTTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGtttca > 1:4392976/1‑55 (MQ=255) cgcgAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGttt < 1:5358661/55‑1 (MQ=255) cgcgAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGttt < 1:828371/55‑1 (MQ=255) cgcgAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGttt < 1:2828699/55‑1 (MQ=255) cgcgAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGttt < 1:1347161/55‑1 (MQ=255) cgcgAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTgggc > 1:813981/1‑61 (MQ=255) cgcgAATGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTggg > 1:3676836/1‑58 (MQ=255) gcgGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGc > 1:2286505/1‑70 (MQ=255) ggATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAAt > 1:2359578/1‑71 (MQ=255) aTGTCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGGGCCTCTTCCAGCAGTTc < 1:2320369/71‑1 (MQ=255) aTGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGAt < 1:1610945/49‑1 (MQ=255) tGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGa < 1:472575/41‑1 (MQ=255) tGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCt < 1:2910283/71‑1 (MQ=255) tCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGtttct > 1:199845/1‑47 (MQ=19) tCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGGGCCTCTTCCAGCAGTTcctg > 1:5068033/1‑68 (MQ=255) tCGTGCGCGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGGGCCTCTTCCAGCAGTTcctg > 1:2990150/1‑68 (MQ=255) tCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGAt < 1:3395182/59‑1 (MQ=255) tCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGAt < 1:287885/59‑1 (MQ=255) aGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTcc < 1:89427/70‑1 (MQ=255) gCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAg < 1:5613530/71‑1 (MQ=255) cGAAGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGGGCCTCTTCCAGCAGTTCCTGTTCCAgc > 1:2203378/1‑70 (MQ=255) aGTATTTACCGCCAAACTGCGCGCCAAGACCGAGTTTCTGGGCCTCTTCCAGCAGTTCCTGTTCCAgctg < 1:4441711/70‑4 (MQ=255) aGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCtttt > 1:2533073/1‑62 (MQ=255) aGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCtttt > 1:4026848/1‑62 (MQ=255) gTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCca > 1:5691118/1‑71 (MQ=255) tATTTATCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGc < 1:1991901/51‑1 (MQ=255) tATTTACCGCCAAACTGCGCGCCAAGACCGAGtttc < 1:2963042/36‑2 (MQ=12) tATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGc < 1:5644418/51‑1 (MQ=255) tATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGc < 1:3916370/51‑1 (MQ=255) tATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTc < 1:5335744/68‑1 (MQ=255) tATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTc < 1:2062810/68‑1 (MQ=255) tATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTc < 1:170607/68‑1 (MQ=255) tATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCcac < 1:4015888/71‑1 (MQ=255) aTTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGAt > 1:4286222/1‑46 (MQ=255) aTTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCcac > 1:2587251/1‑70 (MQ=255) tttACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGAt < 1:3136481/45‑1 (MQ=255) tttACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGAt < 1:548298/45‑1 (MQ=255) tACCACCAAACTGCGCCCCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATcg > 1:4337732/1‑71 (MQ=255) tACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGAt < 1:2772074/43‑1 (MQ=255) tACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGAt < 1:791727/43‑1 (MQ=255) tACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCttt > 1:2086504/1‑55 (MQ=255) tACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAg > 1:4687202/1‑61 (MQ=255) tACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATcg > 1:5206192/1‑71 (MQ=255) tACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATcg > 1:4518929/1‑71 (MQ=255) accaccAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCa > 1:1342216/1‑47 (MQ=255) accaccAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCa > 1:3226614/1‑47 (MQ=255) caccaAACTGCGCACCCAGACCAAGATTTTGCGCTTCGAt < 1:3732206/40‑1 (MQ=255) caccaAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCgg < 1:4782366/71‑1 (MQ=255) ccaAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGaa > 1:4364390/1‑71 (MQ=255) caAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAAc > 1:2584735/1‑71 (MQ=255) aaaCTGCGCGCCAAGACCGAGTTTCTGGGCCTCTTCCAGCAGTTCCTGTTCCAGCTGGACATCGCGGAACg > 1:3177950/1‑71 (MQ=255) aaCTGCGCGCCAAGACCGAGTTTCTGGGCCTCTTCCAGCAGTTCCTGTTCCAGCTGGACATCGCGGAACGc > 1:5115266/1‑71 (MQ=255) aaCTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCtttt < 1:3385621/48‑1 (MQ=255) aaCTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGtt > 1:2185789/1‑55 (MQ=255) aaCTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGc > 1:3749115/1‑71 (MQ=255) aCTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTc < 1:2063972/49‑1 (MQ=255) aCTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCca < 1:1751104/57‑1 (MQ=255) aCTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGcc > 1:310655/1‑71 (MQ=255) cTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTc > 1:4284153/1‑48 (MQ=255) cgcACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTg < 1:1220813/69‑1 (MQ=255) cACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGaa > 1:1358784/1‑61 (MQ=255) cccAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCca < 1:2871839/49‑1 (MQ=255) cccAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCca < 1:4259719/49‑1 (MQ=255) ccAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCca < 1:4973735/48‑1 (MQ=255) ccAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGc > 1:4095523/1‑71 (MQ=255) ccAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGc > 1:5649973/1‑71 (MQ=255) cAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCt < 1:5475389/71‑1 (MQ=255) cAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCt > 1:4954005/1‑71 (MQ=255) | CGCCGTGACGTGGCAGGCGAATCACGCGGATGTCGTGAGCGAAGTATTTACCACCAAACTGCGCACCCAGACCAAGATTTTGCGCTTCGATCAGCAATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCT > Exported/1015147‑1015284 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |