| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,015,310 | A→G | 14.6% | Y253Y (TAT→TAC) | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,015,310 | 0 | A | G | 14.6% | 176.2 / 19.5 | 75 | Y253Y (TAT→TAC) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base A (23/41); new base G (4/7); total (27/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGTTCGTTTCTGCAGAAGTTCCACCAATA > Exported/1015242‑1015373 | aaTTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTtcatca > 1:2610039/1‑69 (MQ=255) cTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTAt > 1:750885/1‑71 (MQ=255) ttttCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTAtt > 1:3862745/1‑70 (MQ=255) ttCCAGCTGGACATCGCGGAACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTTTATCGTAATagt < 1:17031/68‑3 (MQ=255) ttCCAGCTGGACATCGCGGAACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTaa > 1:3190261/1‑63 (MQ=255) ttCCAGCTGGACATCGCGGAACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATagtg < 1:4404743/69‑4 (MQ=255) ttCCAGCTGGACATCGCGGAACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATagtg < 1:5569910/69‑4 (MQ=255) ttCCAGCTGGACATCGCGGAACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATagtg < 1:2284907/69‑4 (MQ=255) ttCCAGCTGGACATCGCGGAACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATagt < 1:4515649/68‑3 (MQ=255) ttCCAGCTGGACATCGCGGAACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATagt < 1:4292728/68‑3 (MQ=255) ttCCAGCTGGACATCGCGGAACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTAATagt < 1:4736138/68‑3 (MQ=255) tCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTcgc > 1:983451/1‑71 (MQ=255) ttCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAg > 1:4799669/1‑71 (MQ=255) ccACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTtcatca > 1:5571181/1‑53 (MQ=255) ccACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGc > 1:3446307/1‑70 (MQ=255) ccACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGc > 1:404647/1‑70 (MQ=255) aTCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAg > 1:4489761/1‑52 (MQ=255) aTCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGtt > 1:4543378/1‑71 (MQ=255) tCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGttt < 1:3077542/71‑1 (MQ=255) tCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGttt < 1:1834510/71‑1 (MQ=255) tCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGttt > 1:4235186/1‑71 (MQ=255) tCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGttt < 1:694299/71‑1 (MQ=255) gcgGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGaa > 1:2008294/1‑61 (MQ=255) gcgGAACGCCTGACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTa > 1:5257797/1‑48 (MQ=255) gcgGAACGCCCGACCATGTTCGTTCCCTTCCGTCGGCAGTTCATCGTa > 1:3393111/1‑48 (MQ=19) cgGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCAc < 1:2302416/71‑1 (MQ=255) ggAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAg < 1:2423036/47‑1 (MQ=255) aaCGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTa > 1:2587117/1‑47 (MQ=255) aaCGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTa > 1:5109643/1‑47 (MQ=255) aaCGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTa > 1:1589301/1‑47 (MQ=255) gCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGtttt < 1:5389410/71‑1 (MQ=255) ccTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGtttt < 1:4389633/70‑1 (MQ=255) ccTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGtttt > 1:2418531/1‑70 (MQ=255) ccTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGtttt > 1:1930855/1‑70 (MQ=255) aCCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGt < 1:3751543/71‑1 (MQ=255) cGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGTTc < 1:1029168/71‑1 (MQ=255) tGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGtttt < 1:2469337/62‑1 (MQ=255) tGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGtttt < 1:1056117/62‑1 (MQ=255) tGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGtttt < 1:4152522/62‑1 (MQ=255) gCTCATTCCCTTCCGTTGGCAGTTCATCATAGTAt > 1:1864333/1‑35 (MQ=255) gCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGtt < 1:4769520/59‑1 (MQ=255) gCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGTTCGtt > 1:2281909/1‑71 (MQ=255) gCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGTTCGtt > 1:5600145/1‑71 (MQ=255) cATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGcc < 1:3693853/44‑1 (MQ=255) aTTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGTAAGCCa < 1:739269/44‑1 (MQ=255) aTTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCa < 1:1418580/44‑1 (MQ=255) aTTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCa < 1:62990/44‑1 (MQ=255) aTTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGTTCGTTTc < 1:4961902/69‑1 (MQ=255) aTTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGTTCGTTTc < 1:898968/69‑1 (MQ=255) aTTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGTTCGTTTCt > 1:147904/1‑70 (MQ=255) ttCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCa < 1:3998619/43‑1 (MQ=255) ttCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGTTCGTTTCt > 1:2425928/1‑69 (MQ=255) tCCCTTCCGTCGGCAGTTCATCGTAATAGTGAGCGCTTGCTAACTTGACGGTTTTCAGGTTGGTTTccg > 1:2212573/1‑67 (MQ=255) ccTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGTTCGTTTCTGCAg < 1:1177108/70‑1 (MQ=255) ccTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGTTCGTTTCTGCAg < 1:5404010/70‑1 (MQ=255) ccTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGTTCGTTTCTGCAg < 1:3626555/70‑1 (MQ=255) ccTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGTTCGTTTCTGCAg < 1:5056388/70‑1 (MQ=255) cTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGTTCGTTTCTGCAg < 1:4485363/69‑1 (MQ=255) ttCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTc < 1:2943563/43‑1 (MQ=255) cGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGTTCGTTTCTGCAGAAGTTc > 1:3795441/1‑71 (MQ=255) cGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGTTCGTTTCTGCAGAAGTTc < 1:1543930/71‑1 (MQ=255) ttGTCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGtttt < 1:3827362/46‑1 (MQ=255) ttGGCAGTTCATCATAGTATTTCGCGGAAGCCAGtt < 1:963552/36‑1 (MQ=255) ttGGCAGTTCATCATAGTATTTCGCGGAAGCCAGtt < 1:3484738/36‑1 (MQ=255) ttGGCAGTTCATCATAGTATTTCGCGGAAGCCAGtt < 1:4443503/36‑1 (MQ=255) ttGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGtttt < 1:4578205/46‑1 (MQ=255) ttGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGTTCGTTTCTGCAGAAGTTcca > 1:2912347/1‑71 (MQ=255) tGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGTTCGTTTCTGCAGAAGTTccac > 1:5304233/1‑71 (MQ=255) aGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGTTGGTTTCTGCAGAAGTTCCACCAAt < 1:5504283/71‑1 (MQ=255) aGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGTTCGTTTCTGCAGAAGTTCCACCAAt < 1:2853663/71‑1 (MQ=255) aGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGTTCGTTTCTGCAGAAGTTCCACCAAt < 1:1879244/71‑1 (MQ=255) aGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGTTCGTTTCTGCAGAAGTTCCACCAAt < 1:3099846/71‑1 (MQ=255) gTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGTTCGTTTCTGCAGAAGt < 1:1520600/61‑1 (MQ=255) gTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGTTCGTTTCTGCAGAAGTTCCACCAATa < 1:4434323/71‑1 (MQ=255) ttCATCATAGTATTTCGCGGAAGCCAGTTTCACCGTTTTa < 1:2830031/40‑1 (MQ=255) ttCATCATAGTATTTCGCGGAAGCCAGTTTCACCGTTTTa < 1:275753/40‑1 (MQ=255) ttCATCATAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGTTc < 1:5404596/46‑1 (MQ=255) catcatAGTATTTCGCGGAAGCCAGTTTCACCGTTTTaa > 1:1023773/1‑39 (MQ=255) catcatAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGTTCGttt > 1:726121/1‑48 (MQ=255) catcatAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGTTCGt > 1:4461205/1‑46 (MQ=255) catcatAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGTTCGTTTCTGc > 1:3788461/1‑52 (MQ=255) catcatAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGTTCGTTTCTGc > 1:2663206/1‑52 (MQ=255) catcatAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGTTCGTTTCTGc > 1:5545209/1‑52 (MQ=255) tcatAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGTTCGTTTCTGCAg < 1:2509231/52‑1 (MQ=255) tcatAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGTTCGTTTCTGCAg < 1:5636974/52‑1 (MQ=255) tcatAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGTTCGTTTCTGCAg < 1:5043603/52‑1 (MQ=255) atAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGTTCGTTTCTGCAGAAGTTcc > 1:4293401/1‑57 (MQ=255) | AATTCTTTTTCCAGTTCCACATCGCGGAACGCCTGACCGTGCTCATTCCCTTCCGTTGGCAGTTCATCATAGTATTTCGCGGAAGCCAGTTTCACCGTTTTAAGGTTCGTTTCTGCAGAAGTTCCACCAATA > Exported/1015242‑1015373 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |