| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,015,430 | A→G | 17.8% | V213V (GTT→GTC) | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,015,430 | 0 | A | G | 17.8% | 177.6 / 31.0 | 84 | V213V (GTT→GTC) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base A (29/40); new base G (5/10); total (34/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.77e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01 | |||||||||||
GTTCCACCAATAACGAACGCAATATGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGT > Exported/1015362‑1015497 | gTTCCACCAATAACGAACGCAATATGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAAc < 1:2277619/71‑1 (MQ=255) accaATAACGAACGCAATATGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGt < 1:814153/71‑1 (MQ=255) accaATAACGAACGCAATATGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGt < 1:1023314/71‑1 (MQ=255) tAACGAACGCAATATGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAAttt < 1:3473114/71‑1 (MQ=255) acgaacgCAATATGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAAttttt < 1:2967324/71‑1 (MQ=255) acgaacgCAATATGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAAttttt < 1:4536778/71‑1 (MQ=255) cgCAATATGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCa > 1:794365/1‑68 (MQ=255) cgCAATATGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGtt < 1:5365493/71‑1 (MQ=255) gCAATATGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAAttt < 1:3748241/63‑1 (MQ=255) gCAATATGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAAttt < 1:1857489/63‑1 (MQ=255) gatatGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGtttgc < 1:4848153/70‑3 (MQ=255) tatGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTgccg > 1:631194/1‑70 (MQ=255) tatGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAAc < 1:2984148/49‑1 (MQ=255) tatGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTcccc < 1:4375839/71‑1 (MQ=255) tatGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTcccc > 1:5045484/1‑71 (MQ=255) atGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCaa > 1:551921/1‑47 (MQ=255) atGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAAc > 1:1898184/1‑48 (MQ=255) tGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGttt > 1:1269513/1‑65 (MQ=255) tACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCa < 1:4972865/58‑1 (MQ=255) tACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGt < 1:4563966/71‑1 (MQ=255) tACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGt < 1:142070/71‑1 (MQ=255) tACGGAGGACAGGCCGCCGTACCCAGCGTGAGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGt < 1:2622271/71‑1 (MQ=255) cGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTa > 1:3833740/1‑48 (MQ=255) cGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGt > 1:1383487/1‑69 (MQ=255) cGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGt > 1:4328100/1‑69 (MQ=255) gaggaCAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAg > 1:3164576/1‑43 (MQ=255) gaggaCAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAg > 1:2282417/1‑70 (MQ=255) ggaCAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCa < 1:5363181/52‑1 (MQ=255) ggaCAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTaa > 1:666597/1‑71 (MQ=255) ggcAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAg < 1:4801432/65‑1 (MQ=255) gaCAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAAttttt < 1:2588765/49‑1 (MQ=255) gaCAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAAttttt < 1:799995/49‑1 (MQ=255) gaCAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAAttttt < 1:1109754/49‑1 (MQ=255) gaCAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAAttttt < 1:4864288/49‑1 (MQ=255) gcAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGtttg < 1:1713811/54‑2 (MQ=255) cAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGc > 1:4211564/1‑71 (MQ=255) gccgccGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTc < 1:867798/60‑1 (MQ=255) cgccgTACCCAGCGTGCGCATCTTCTCAACCAGGTAAttttt < 1:3312633/42‑1 (MQ=255) cgccgTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCa < 1:5326349/44‑1 (MQ=255) cgccgTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCa < 1:1375564/44‑1 (MQ=255) ccgTACCCAGCGTGCGCATCTTCTCAACCAGGTaa < 1:1319913/35‑1 (MQ=255) ccgTACCCAGCGTGCGCATCTTCTCAACCAGGTAAttttt < 1:2836546/40‑1 (MQ=255) ccgTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTc < 1:3432428/41‑1 (MQ=255) ccgTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGt > 1:2798167/1‑44 (MQ=255) ccgTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGt > 1:2249303/1‑44 (MQ=255) ccgTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGtt > 1:5261989/1‑71 (MQ=255) ccgTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGtt < 1:3999617/71‑1 (MQ=255) gTACCGAGGGTACACATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGATTTGGTTTc > 1:5678796/1‑71 (MQ=255) gTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTc > 1:3451956/1‑71 (MQ=255) gTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTc > 1:3458471/1‑71 (MQ=255) aCCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAgca > 1:4605434/1‑54 (MQ=255) cAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGa > 1:2520491/1‑70 (MQ=255) cAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGAt < 1:2061669/71‑1 (MQ=255) cAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGAt < 1:2068252/71‑1 (MQ=255) aGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGa > 1:1410421/1‑69 (MQ=255) gTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATagag < 1:3478245/71‑1 (MQ=255) gTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATa > 1:1316827/1‑68 (MQ=255) tGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTgg < 1:2885404/57‑1 (MQ=255) tGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAgaga < 1:1084939/71‑1 (MQ=255) tGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAgaga < 1:3181154/71‑1 (MQ=255) cgcATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGAt < 1:4789657/70‑1 (MQ=255) cgcATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATa > 1:5430970/1‑71 (MQ=255) cATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAg < 1:1173456/42‑1 (MQ=255) cATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATAc < 1:2189701/70‑1 (MQ=255) aTCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGt > 1:5617337/1‑71 (MQ=255) aTCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGt > 1:377443/1‑71 (MQ=255) ttttctCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTg < 1:3261203/48‑1 (MQ=255) ttttctCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGt < 1:4397292/50‑1 (MQ=255) ttctCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGtttt > 1:399374/1‑71 (MQ=255) ttctCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTAGTAGAGATACGttt > 1:3581867/1‑68 (MQ=255) ttctCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGa > 1:466936/1‑57 (MQ=255) ttctCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGa > 1:5276588/1‑57 (MQ=255) ttctCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGa > 1:5182584/1‑57 (MQ=255) ttctCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCt > 1:3010719/1‑70 (MQ=255) tctcGACGAGGAAGTTTTTCAGTTTGCCGGGCGTCAGCATGGCTTTGGTTTCCTGGTAGAGAt < 1:4334658/63‑1 (MQ=255) tctcGACGAGGAAGTTTTTCAGTTTGCCGGGCGTCAGCAGGGCTTTGGTTTCCTGGTAGAGAt < 1:5227161/63‑1 (MQ=255) tctcGACGAGGAAGTTTTTCAGTTTGCCGGGCGTCAGCAGGGCTTTGGTTTCCTGGTAGAGAt < 1:460578/63‑1 (MQ=255) tctcGACGAGGAAGTTTTTCAGTTTGCCGGGCGTCAGCAGGGCTTTGGTTTCCTGGTAGAGAt < 1:2717369/63‑1 (MQ=255) tctcGACGAGGAAGTTTTTCAGTTTGCCGGGCGTCAGCAGGGCTTTGGTTTCCTGGTAGAGAt < 1:2530501/63‑1 (MQ=255) tctcAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTaa > 1:3294336/1‑40 (MQ=255) tctcAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATAc < 1:4694561/65‑1 (MQ=255) tctcAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATAc < 1:5426159/65‑1 (MQ=255) ctcAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCt < 1:4258569/68‑1 (MQ=255) tcAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATagag > 1:4370668/1‑59 (MQ=255) cAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCttt > 1:105406/1‑43 (MQ=255) aaCCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTaa > 1:2427887/1‑36 (MQ=255) aaCCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGt < 1:3086951/68‑1 (MQ=255) aaCCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGt < 1:3157312/68‑1 (MQ=255) aaCCAGGCAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGt < 1:5590001/68‑1 (MQ=255) | GTTCCACCAATAACGAACGCAATATGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGT > Exported/1015362‑1015497 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |