| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 217,977 | C→T | 31.9% | intergenic (‑69/‑37) | yafU ← / → yafF | predicted inner membrane protein/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 217,977 | 0 | C | T | 31.9% | 246.1 / 154.9 | 194 | intergenic (‑69/‑37) | yafU/yafF | predicted inner membrane protein/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (60/72); new base T (24/38); total (84/110) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.38e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.70e-01 | |||||||||||
Only 100 of 195 total aligned reads displayed.
ATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGA > Exported/217910‑218043 | tcagTTCTGCTGATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGATAATAAg < 1:914249/68‑1 (MQ=255) tGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATa < 1:2511962/68‑1 (MQ=255) cagTTCTGCTGATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGc > 1:1800812/3‑71 (MQ=255) gTTCTGCTGATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTg < 1:2634944/71‑1 (MQ=255) gTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCt > 1:3081042/1‑70 (MQ=255) gTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTg < 1:1500413/71‑1 (MQ=255) gTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATaa < 1:4777835/67‑1 (MQ=255) tCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGc > 1:2127757/1‑67 (MQ=255) ctgctgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGca < 1:1709404/70‑1 (MQ=255) ctgctgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGca < 1:3278884/70‑1 (MQ=255) ctgctgATTTAACCGCAGAAAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGcac > 1:775220/1‑71 (MQ=255) ctgctgATTTAACCGCAGAAAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGcac > 1:4592529/1‑71 (MQ=255) tgctgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTg < 1:3364865/67‑1 (MQ=255) tgctgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTg < 1:2292927/67‑1 (MQ=255) tgctgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGCCATGTGGAGAATAAGCTg < 1:1386567/67‑1 (MQ=255) gctgATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGaa < 1:888866/59‑1 (MQ=255) gATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGcactg < 1:2472351/68‑3 (MQ=255) aTTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAg > 1:2922580/1‑59 (MQ=255) aTTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGt > 1:4721210/1‑71 (MQ=255) ttAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACg > 1:1863310/1‑46 (MQ=25) ttAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGt > 1:3662270/1‑69 (MQ=255) tAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTg > 1:2033247/1‑71 (MQ=255) aaCCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTg < 1:3691445/70‑1 (MQ=255) aCCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTg < 1:1220340/69‑1 (MQ=255) ccGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGcac < 1:789490/59‑1 (MQ=255) cGCAGAAAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGgag < 1:3727569/46‑1 (MQ=255) gCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGt < 1:320840/71‑1 (MQ=255) ctgagaAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAg > 1:2269167/3‑50 (MQ=37) ctgagaAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTg > 1:1464334/3‑71 (MQ=255) cAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGcactg < 1:5480294/58‑3 (MQ=255) cAGAAAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGca > 1:316088/1‑55 (MQ=255) agagAAGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTg < 1:4280936/52‑1 (MQ=255) agagAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTgg < 1:5525763/71‑1 (MQ=255) gaaaaGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGt < 1:1425037/68‑1 (MQ=255) aaaaGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATaa < 1:3403752/44‑1 (MQ=255) gaAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTaa > 1:5564911/1‑71 (MQ=255) aaaGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAg > 1:4014494/2‑46 (MQ=255) aaattCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACg < 1:2987081/61‑1 (MQ=255) aaGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTg < 1:1060063/60‑1 (MQ=38) aaGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACg < 1:2818678/64‑1 (MQ=255) aaGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaat < 1:4857679/71‑1 (MQ=255) aaGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGc > 1:3090713/1‑46 (MQ=255) aaGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGc > 1:1007590/1‑46 (MQ=255) aaGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaa > 1:4839950/1‑70 (MQ=255) aGTTCGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaat < 1:4325223/70‑1 (MQ=255) aGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTaatg < 1:231813/71‑1 (MQ=255) attCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATa < 1:2619449/40‑1 (MQ=38) attCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatg > 1:3880512/2‑70 (MQ=255) ttCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGca > 1:4806457/1‑47 (MQ=255) cGCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatgaat > 1:408426/1‑70 (MQ=39) gCCACAGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatgaatg > 1:4191845/1‑70 (MQ=39) gCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatg < 1:1666448/66‑1 (MQ=255) gCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatg < 1:1526480/66‑1 (MQ=255) ccACAGCGATCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAAtgaatgaa < 1:3373769/71‑1 (MQ=255) ccACAGCAATCCGAAACCACTGGCACGTGGATAATAAGCTGCACAGGCGTCTGGACGt < 1:1691448/58‑1 (MQ=255) ccACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAAtgaatgaa < 1:949904/71‑1 (MQ=255) cacaGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACg > 1:258264/1‑56 (MQ=38) cacaGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatgaa < 1:3630250/66‑1 (MQ=39) cacaGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatgaa < 1:2563555/66‑1 (MQ=39) acaGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAga > 1:1180948/1‑71 (MQ=255) caGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAgac < 1:1755055/71‑1 (MQ=255) aGCAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAatgaatga > 1:5182723/1‑66 (MQ=39) gcgaTCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAgacga < 1:1558165/68‑1 (MQ=255) gCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAgacga > 1:5384363/1‑71 (MQ=255) gCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAgacga > 1:2762749/1‑71 (MQ=255) cAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaatgaa < 1:93979/61‑1 (MQ=38) cAATCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAAtgaatgaa < 1:1723496/65‑1 (MQ=39) cAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAgacga < 1:4896869/70‑1 (MQ=255) aaTCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCg < 1:3353800/42‑1 (MQ=255) aTCCGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTgg < 1:2915520/52‑1 (MQ=255) aTCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAgac < 1:5408078/66‑1 (MQ=255) tCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGt < 1:5121478/41‑1 (MQ=25) tCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGt < 1:4398494/41‑1 (MQ=25) tCCGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACtg > 1:3170285/1‑69 (MQ=39) tCCGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAgac < 1:1304847/65‑1 (MQ=255) tCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGt < 1:1212738/41‑1 (MQ=255) ccGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAAtgaatgaa > 1:2423762/1‑61 (MQ=255) ccGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAAtgaatgaa > 1:2045651/1‑61 (MQ=255) ccGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAAtgaatgaa < 1:4957015/61‑1 (MQ=255) ccGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTAc > 1:3945071/1‑70 (MQ=255) ccGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTAc > 1:3331895/1‑70 (MQ=255) cGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAAtgaatgaa < 1:2216808/60‑1 (MQ=255) cGAAATCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAAtgaatgaa < 1:349716/60‑1 (MQ=255) cGAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCa > 1:3812282/1‑70 (MQ=38) cGAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCa > 1:3648158/1‑70 (MQ=255) gAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAgacga > 1:5029932/1‑64 (MQ=255) gAAACCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAgacga > 1:85915/1‑64 (MQ=255) gAAACCACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAAtgaatgaa < 1:1950028/59‑1 (MQ=255) aaaCCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACaaaa > 1:1000416/1‑71 (MQ=255) atcACTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAAt > 1:4553976/3‑70 (MQ=255) aCCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCaaaa > 1:716916/1‑69 (MQ=39) aCCACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAAt > 1:4678397/1‑70 (MQ=255) ccACTGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGt < 1:2369899/42‑1 (MQ=37) aCTGGCACGTGGAGAATAAGCTGCACTGGCGTCTggc > 1:2237848/1‑36 (MQ=39) aCTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATaag > 1:1143779/1‑70 (MQ=255) aCTGGCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTAATGAATGAAGACGACTGCAAAATaag > 1:1612584/1‑70 (MQ=255) tGGCATGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaat < 1:2861152/44‑1 (MQ=38) tGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAgac < 1:68454/54‑1 (MQ=255) gCACGTGGAGAATAAGCTGCACTGGCGTCTGGACGTGGTaa > 1:3668275/1‑41 (MQ=255) gCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAagaaga < 1:5262444/70‑1 (MQ=255) | ATAAATTCTATGGTTCTGCTGATTTAACCGCAGAGAAGTTCGCCACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGA > Exported/217910‑218043 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |