| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,015,595 | C→G | 12.5% | V158V (GTG→GTC) | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,015,595 | 0 | C | G | 12.5% | 160.5 / 13.4 | 64 | V158V (GTG→GTC) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base C (33/23); new base G (3/5); total (36/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.82e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.94e-01 | |||||||||||
AGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGA > Exported/1015525‑1015665 | agagGAATTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTc < 1:2360023/71‑1 (MQ=255) gagGAATTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCa > 1:940671/1‑71 (MQ=255) ggAATTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCAct < 1:3024302/71‑1 (MQ=255) tttGTATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCActtctt < 1:4432141/71‑1 (MQ=255) ttGTATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCActtctt > 1:4243759/1‑70 (MQ=255) ttGTATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCActtctt > 1:4250465/1‑70 (MQ=255) tATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTtata > 1:142132/1‑71 (MQ=255) tATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTtat < 1:5629182/70‑1 (MQ=255) aTTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTtata > 1:4939793/1‑70 (MQ=255) tcgtcgCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCAc > 1:2745223/1‑59 (MQ=255) tcgtcgCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACa > 1:2891749/1‑70 (MQ=255) gtcgCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACatat < 1:1752028/71‑1 (MQ=255) gCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCAc < 1:1697430/54‑1 (MQ=255) aTCAACGGCATAATGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAgcg < 1:963476/71‑1 (MQ=255) aaCGGCATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAgcggcg > 1:1751716/1‑71 (MQ=255) aaCGGCATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAgcggcg > 1:4405777/1‑71 (MQ=255) aaCGGCATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAgcggcg > 1:4497177/1‑71 (MQ=255) aaCGGCATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAgcggcg > 1:2116629/1‑71 (MQ=255) aaCGGCATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAgcggc > 1:2999858/1‑70 (MQ=255) cATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGttt < 1:4754688/71‑1 (MQ=255) aTAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTta > 1:5240198/1‑49 (MQ=255) aTAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTta > 1:789653/1‑49 (MQ=255) aTAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGtttt < 1:2929639/71‑1 (MQ=255) aTAAAGATCGATCTGCGCTGGCAAGTTGGTGCCGGTATTCACCTCTTTATACATATCCAGCGGCGCGtttt > 1:5242443/1‑71 (MQ=255) tAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGtttt > 1:1929062/1‑70 (MQ=255) aaGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTc < 1:2103586/37‑1 (MQ=255) aaGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTc < 1:616364/37‑1 (MQ=255) aaGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTc < 1:1565666/37‑1 (MQ=255) aaGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACatat > 1:1238145/1‑53 (MQ=255) aaGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGc > 1:3881137/1‑70 (MQ=255) aaGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGc > 1:1177020/1‑70 (MQ=255) aGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGc > 1:497651/1‑69 (MQ=255) aGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGc > 1:4381132/1‑69 (MQ=255) atcgatcTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGt < 1:2303055/71‑1 (MQ=255) cgatcTGCGCTGGCAGATTGGTGCCGGTATTCACTTCttt > 1:443631/1‑40 (MQ=255) cgatcTGCGCTGGCAGATTGGTGCCGGTATTCACTTCttt > 1:2619794/1‑40 (MQ=255) cgatcTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTta > 1:5604258/1‑41 (MQ=255) cgatcTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTta > 1:253394/1‑41 (MQ=255) cTGCGCTGGCAAGTTGGTGCCGGTATTCACCTCttt < 1:783197/36‑1 (MQ=25) gcgcTGGCAGATTGGTGCCGGTATTCACTTCTTtata > 1:3340704/1‑37 (MQ=255) gcgcTGGCAGATTGGTGCCGGTATTCACTTCTTtata > 1:2252904/1‑37 (MQ=255) cgcTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGa > 1:4642723/1‑70 (MQ=255) cgcTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGa < 1:3757661/70‑1 (MQ=255) cgcTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGAt > 1:4709590/1‑71 (MQ=255) gcTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGAtt < 1:4079060/71‑1 (MQ=255) gcTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGAtt < 1:3908554/71‑1 (MQ=255) gcTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGAtt < 1:465080/71‑1 (MQ=255) gCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCt < 1:1982053/71‑1 (MQ=255) aGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGttt < 1:2697873/48‑1 (MQ=255) aGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAg < 1:1692001/62‑1 (MQ=255) ttGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGat < 1:2594335/71‑1 (MQ=255) ggTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTg > 1:4238739/1‑45 (MQ=255) agTGCCGGTGTTGACCTCTTTGTACATGTCCAGCGCCGCATTCTGTGAATAGCGCAGGTTATCTTCGata < 1:3778340/69‑1 (MQ=255) gTGCCGGTGTTGACCTCTTTGTACATGTCCAGCGCCGCATTCTGTGAATAGCGCAGGTTATCTTCGatat < 1:3461270/70‑1 (MQ=255) gTGCCGGTGTTGACCTCTTTGTACATGTCCAGCGCCGCATTCTGTGAATAGCGCAGGTTATCTTCGatat < 1:4143419/70‑1 (MQ=255) gTGCCGGTGTTGACCTCTTTGTACATGTCCAGCGCCGCATTCTGTGAATAGCGCAGGTTATCTTCGata > 1:5581302/1‑69 (MQ=255) gTGCCGGTGGTGACCTCTTTGTACATGTCCAGCGCCGCATTCTGTGAATAGCGCAGGTTATCTTCGatat < 1:3454570/70‑1 (MQ=255) gTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGatat < 1:3564154/70‑1 (MQ=255) gTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGatat < 1:1775278/70‑1 (MQ=255) ggTGTTGACCTCTTTGTACATGTCCAGCGCCGCATTCTGTGAATAGCGCAGGTTATCTTCGata > 1:1171213/1‑64 (MQ=255) ggTGTTGACCTCTTTGTACATGTCCAGCGCCGCATTCTGTGAATAGCGCAGGTTATCTTCGata > 1:534164/1‑64 (MQ=255) ggTGTTGACCTCTTTGTACATGTCCAGCGCCGCATTCTGTGAATAGCGCAGGTTATCTTCGATATAGgtgt < 1:4440195/71‑1 (MQ=255) gTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTc < 1:2761376/59‑1 (MQ=255) gTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGtt < 1:4724312/71‑1 (MQ=255) aTTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGa > 1:1563653/1‑39 (MQ=255) aTTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTa > 1:171411/1‑52 (MQ=255) ttCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTtata < 1:5054075/71‑1 (MQ=255) tCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGtt > 1:294279/1‑67 (MQ=255) cACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTc > 1:5493322/1‑54 (MQ=255) cACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGa > 1:609669/1‑71 (MQ=255) cACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGa > 1:1504845/1‑71 (MQ=255) cACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGa > 1:2754096/1‑71 (MQ=255) | AGAGGAATTTGTATTCGTCGCCATCAACGGCATAAAGATCGATCTGCGCTGGCAGATTGGTGCCGGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGA > Exported/1015525‑1015665 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |