| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,015,655 | A→G | 21.8% | T138T (ACT→ACC) | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,015,655 | 0 | A | G | 21.8% | 99.0 / 24.8 | 55 | T138T (ACT→ACC) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base A (28/15); new base G (4/8); total (32/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.53e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.76e-01 | |||||||||||
GGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTAC > Exported/1015589‑1015725 | ggTGTTGACCTCTTTGTACATGTCCAGCGCCGCATTCTGTGAATAGCGCAGGTTATCTTCGATATAGgtgt < 1:4440195/71‑1 (MQ=255) gTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGtt < 1:4724312/71‑1 (MQ=255) ttCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTtata < 1:5054075/71‑1 (MQ=255) tCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGtt > 1:294279/1‑67 (MQ=255) cACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGa > 1:2754096/1‑71 (MQ=255) cACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGa > 1:1504845/1‑71 (MQ=255) cACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGa > 1:609669/1‑71 (MQ=255) cttTGTACATGTCCAGCGCCGCATTCTGTGAATAGCGCAGGTTATCTTCGATATAGGTGTTATAGACGcct > 1:3645695/1‑70 (MQ=255) tataCATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACcgc < 1:1219466/69‑1 (MQ=255) tataCATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACcgc < 1:4172446/69‑1 (MQ=255) taCATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCgcgcgc > 1:4243451/1‑71 (MQ=255) aCATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGtt < 1:5163592/55‑1 (MQ=255) aCATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGtt < 1:3652130/55‑1 (MQ=255) aCATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGtt < 1:931394/55‑1 (MQ=255) aCATATCCAGCGGCGCGTTTTGCGACTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCgcgcgc > 1:3526384/1‑70 (MQ=255) cATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCa < 1:4582949/71‑1 (MQ=255) atCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAgcg > 1:3766262/1‑71 (MQ=255) tCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATaa > 1:3536829/1‑45 (MQ=255) tCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGaca > 1:3149096/1‑57 (MQ=255) ccAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGcgcc > 1:5688093/1‑71 (MQ=255) ccAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGcgcc > 1:1071204/1‑71 (MQ=255) ccAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGcgcc > 1:1919440/1‑71 (MQ=255) cAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGcgccg > 1:967218/1‑71 (MQ=255) cAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGcgccg > 1:4531729/1‑71 (MQ=255) gcggcgCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGac < 1:760103/52‑1 (MQ=255) cggcgCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCtt < 1:2243750/71‑1 (MQ=255) ggcgCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGacac > 1:389997/1‑52 (MQ=255) tttGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTTCATcacca > 1:163289/1‑71 (MQ=255) tttGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTTCATcacca > 1:327459/1‑71 (MQ=255) ttGCGAGTAGCGCAGATTATCTTCGATATAAGTGTta > 1:4225252/1‑37 (MQ=255) ttGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTTCATcaccac > 1:4547975/1‑71 (MQ=255) gCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGa < 1:1262096/39‑1 (MQ=255) aGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAgcgc < 1:3941957/52‑1 (MQ=255) gTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCgg > 1:1569488/1‑71 (MQ=255) cgcAGGTTATCTTCGATATAGGTGTTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCa < 1:2678046/71‑1 (MQ=255) cAGGTTATCTTCGATATAGGTGTTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCaca > 1:5239332/1‑69 (MQ=255) cAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATa > 1:707321/1‑71 (MQ=255) ggttATCTTCGATATAGGTGTTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCacac > 1:2203505/3‑70 (MQ=255) aTTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTcc < 1:141177/65‑1 (MQ=255) ttATCCTCGATATAAGTGTTATAGACACCGCGCGcc > 1:2181820/1‑36 (MQ=255) ttATCCTCGATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTTCATcacc > 1:2000193/1‑53 (MQ=255) tATCTTCGATATAGGTGTTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACgcgc > 1:2326450/1‑71 (MQ=255) aTCTTCGATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTtca > 1:4562658/1‑46 (MQ=255) tCTTCGATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTg > 1:3654795/1‑71 (MQ=255) cTTCGATATAGGTGTTATAGACGCCTTTCGACAGCGTTTCTTCATcaccgc < 1:963907/51‑3 (MQ=255) cTTCGATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTTCATcaccaccac < 1:2301525/54‑1 (MQ=255) gATATAGGTGTTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCtt < 1:1704682/71‑1 (MQ=255) gATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTtcat > 1:4994944/1‑41 (MQ=255) gATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTtcat > 1:5581721/1‑41 (MQ=255) gATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCAt > 1:1070353/1‑58 (MQ=255) atatAGGTGTTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCttt < 1:1647556/71‑1 (MQ=255) atatAGGTGTTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCttt < 1:1357801/71‑1 (MQ=255) atatAGGTGTTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCttt < 1:2118962/71‑1 (MQ=255) tataGGTGTTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCttt < 1:1981250/70‑1 (MQ=255) ataAGTGTTATAGACACCGCGCGCCAGCGCCGCTCCATCACCACCACCGGTCCATACACGCTGCCCttttt > 1:4085079/1‑71 (MQ=255) taAGTGTTATAGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCa < 1:4237096/53‑1 (MQ=255) aGTGTTATAGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTAc > 1:2649153/1‑71 (MQ=255) | GGTATTCACTTCTTTATACATATCCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTAC > Exported/1015589‑1015725 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |