| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,015,706 | T→C | 21.7% | V121V (GTA→GTG) | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,015,706 | 0 | T | C | 21.7% | 130.1 / 32.1 | 69 | V121V (GTA→GTG) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base T (29/25); new base C (3/12); total (32/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.83e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.11e-01 | |||||||||||
CAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCC > Exported/1015637‑1015771 | cAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATa > 1:707321/1‑71 (MQ=255) ggttATCTTCGATATAGGTGTTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCacac > 1:2203505/3‑70 (MQ=255) tATCTTCGATATAGGTGTTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACgcgc > 1:2326450/1‑71 (MQ=255) tCTTCGATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTg > 1:3654795/1‑71 (MQ=255) gATATAGGTGTTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCtt < 1:1704682/71‑1 (MQ=255) gATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCAt > 1:1070353/1‑58 (MQ=255) atatAGGTGTTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCttt < 1:1357801/71‑1 (MQ=255) atatAGGTGTTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCttt < 1:1647556/71‑1 (MQ=255) atatAGGTGTTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCttt < 1:2118962/71‑1 (MQ=255) tataGGTGTTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCttt < 1:1981250/70‑1 (MQ=255) ataAGTGTTATAGACACCGCGCGCCAGCGCCGCTCCATCACCACCACCGGTCCATACACGCTGCCCttttt > 1:4085079/1‑71 (MQ=255) aGTGTTATAGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTAc > 1:2649153/1‑71 (MQ=255) gtgtTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCTTTTTTAcc < 1:5075048/71‑1 (MQ=255) ataGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACcaaca > 1:5127351/1‑70 (MQ=255) taGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACcaacaa > 1:3402511/1‑70 (MQ=255) taGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACcaacaa > 1:2528372/1‑70 (MQ=255) taGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACaat < 1:4727397/71‑1 (MQ=255) gACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACaataa > 1:3811583/1‑71 (MQ=255) acacCGCGCGCCAGCGCCGCTTCATCACCACCCCCGGTCCATACCCGCTGCCCTTTTTTACCAACaataat > 1:520643/1‑71 (MQ=255) cacCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATc < 1:3447993/71‑1 (MQ=255) acCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATcg < 1:2695996/71‑1 (MQ=255) ccGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATcgc > 1:3982361/1‑71 (MQ=255) cgcgcgCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATcg < 1:3658047/69‑1 (MQ=255) gcgcgcCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCgg > 1:232508/1‑71 (MQ=255) cgcgcCAGCGCCGCTTCATCACCACCACCGGTCCATa > 1:435346/1‑37 (MQ=255) cgcgcCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCttttt > 1:5052715/1‑52 (MQ=255) gcCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGccc < 1:3002665/44‑1 (MQ=255) ccAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGccc < 1:4759638/43‑1 (MQ=255) aGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCgg > 1:1551041/1‑70 (MQ=255) cgccgcTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTAt > 1:4722473/1‑71 (MQ=255) gccgcTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACaat > 1:1775721/1‑53 (MQ=255) gccgcTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACaat > 1:5430502/1‑53 (MQ=255) gccgcTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATc > 1:4629508/1‑57 (MQ=255) ccgcTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATc < 1:1063355/70‑1 (MQ=255) cgcTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCt < 1:3515801/71‑1 (MQ=255) cgcTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCt < 1:3896823/71‑1 (MQ=255) tcTTCATCACCGCCGCCGGTCCACACGCGCTGGCCTTTTTTACcgac > 1:1429097/2‑47 (MQ=16) cTTCATCACCACCACCGGTCCATACACGCTGCCCtt < 1:623383/36‑1 (MQ=255) cTTCATCACCACCACCGGTCCATACACGCTGCCCtt < 1:5502752/36‑1 (MQ=255) cTTCATCACCACCACCGGTCCATACACGCTGCCCtt < 1:5361848/36‑1 (MQ=255) ttCATCACCGCCGCCGGTCCACACGCGCTGGCCTTTTTTACCGACGATGATCGCGGTGCCGGTATCCTg < 1:1524142/69‑1 (MQ=255) tcatcaCCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCgg < 1:237579/61‑1 (MQ=255) catcaCCGCCGCCGGTCCACACGCGCTGGCCTTTTTTACCGACGATGATCGCGGTGCCGGTATCCTggc < 1:2661484/69‑3 (MQ=255) catcaCCGCCGCCGGTCCACACGCGCTGGCCTTTTTTACCGACGATGATCGAGGTGCCGGTATCCTgtc < 1:2363479/69‑3 (MQ=255) atcaCCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCgg > 1:2489002/1‑53 (MQ=255) atcaCCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGa > 1:3467340/1‑67 (MQ=255) atcaCCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGa > 1:5142457/1‑67 (MQ=255) atcaCCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAgg < 1:770359/71‑1 (MQ=255) tcaCCGCCGCCGGTCCACACGCGCTGGCCTTTTTTACCGACGATGATCGCGGTGCCGGTATCCTGGCAGGt < 1:243615/71‑1 (MQ=255) tcaCCGCCGCCGGTCCACACGCGCTGGCCTTTTTTACCGACGATGATCGCGGTGCCGGTATCCTGGCAGGt > 1:4035952/1‑71 (MQ=255) tcaCCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGa < 1:646429/66‑1 (MQ=255) accaccaccGGTCCATACACGCTGCCCTTTTTTACCAACaataat > 1:4356031/1‑45 (MQ=255) accaccaccGGTCCATACACGCTGCCCTTTTTTACCAACAATAATcg > 1:1572158/1‑47 (MQ=255) ccaccaccGGTCCATACACGCTGCCCTTTTTTACCAACaata < 1:431388/42‑1 (MQ=255) ccaccaccGGTCCATACACGCTGCCCTTTTTTACCAACAATAATc < 1:1340906/45‑1 (MQ=255) ccgccGGTCCACACGCGCTGGCCTTTTTTACCGACGATGATCGCGGTGCCGGTATCCTGGCAGGTCGGCAg < 1:2558444/68‑1 (MQ=255) ccaccGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAg < 1:1060718/71‑1 (MQ=255) caccGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGa < 1:3155127/71‑1 (MQ=255) caccGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGa < 1:304152/71‑1 (MQ=255) ccGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCgg > 1:3911609/1‑49 (MQ=255) ccGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGaa > 1:1963509/1‑70 (MQ=255) ggTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGaa > 1:3296863/1‑68 (MQ=255) ggTCCACACGCGCTGGCCTTTTTTACCGACGATGATcgc < 1:5383109/39‑1 (MQ=9) gTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCgg < 1:5573713/46‑1 (MQ=255) gTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCgg < 1:2627401/46‑1 (MQ=255) gTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGcc > 1:4816617/1‑71 (MQ=255) gTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGc < 1:37240/70‑1 (MQ=255) tCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCgg < 1:4286053/45‑1 (MQ=255) ccATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTa > 1:3679966/1‑46 (MQ=255) ccATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGa > 1:3388531/1‑52 (MQ=255) aTACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAg < 1:1308118/61‑1 (MQ=255) tACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGc < 1:3271662/65‑1 (MQ=255) | CAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCC > Exported/1015637‑1015771 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |