| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,015,730 | A→G | 27.1% | I113I (ATT→ATC) | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,015,730 | 0 | A | G | 27.1% | 125.1 / 51.3 | 81 | I113I (ATT→ATC) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base A (32/27); new base G (11/11); total (43/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.05e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01 | |||||||||||
Only 100 of 101 total aligned reads displayed.
ATAGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGG > Exported/1015661‑1015799 | ataGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACcaaca > 1:5127351/1‑70 (MQ=255) taGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACcaacaa > 1:2528372/1‑70 (MQ=255) taGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACcaacaa > 1:3402511/1‑70 (MQ=255) taGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACaat < 1:4727397/71‑1 (MQ=255) gACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACaataa > 1:3811583/1‑71 (MQ=255) acacCGCGCGCCAGCGCCGCTTCATCACCACCCCCGGTCCATACCCGCTGCCCTTTTTTACCAACaataat > 1:520643/1‑71 (MQ=255) cacCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATc < 1:3447993/71‑1 (MQ=255) acCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATcg < 1:2695996/71‑1 (MQ=255) ccGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATcgc > 1:3982361/1‑71 (MQ=255) cgcgcgCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATcg < 1:3658047/69‑1 (MQ=255) gcgcgcCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCgg > 1:232508/1‑71 (MQ=255) aGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCgg > 1:1551041/1‑70 (MQ=255) cgccgcTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTAt > 1:4722473/1‑71 (MQ=255) gccgcTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACaat > 1:5430502/1‑53 (MQ=255) gccgcTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACaat > 1:1775721/1‑53 (MQ=255) gccgcTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATc > 1:4629508/1‑57 (MQ=255) ccgcTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATc < 1:1063355/70‑1 (MQ=255) cgcTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCt < 1:3515801/71‑1 (MQ=255) cgcTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCt < 1:3896823/71‑1 (MQ=255) ttCATCACCGCCGCCGGTCCACACGCGCTGGCCTTTTTTACCGACGATGATCGCGGTGCCGGTATCCTg < 1:1524142/69‑1 (MQ=255) tcatcaCCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCgg < 1:237579/61‑1 (MQ=255) catcaCCGCCGCCGGTCCACACGCGCTGGCCTTTTTTACCGACGATGATCGCGGTGCCGGTATCCTggc < 1:2661484/69‑3 (MQ=255) catcaCCGCCGCCGGTCCACACGCGCTGGCCTTTTTTACCGACGATGATCGAGGTGCCGGTATCCTgtc < 1:2363479/69‑3 (MQ=255) atcaCCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCgg > 1:2489002/1‑53 (MQ=255) atcaCCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGa > 1:3467340/1‑67 (MQ=255) atcaCCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGa > 1:5142457/1‑67 (MQ=255) atcaCCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAgg < 1:770359/71‑1 (MQ=255) tcaCCGCCGCCGGTCCACACGCGCTGGCCTTTTTTACCGACGATGATCGCGGTGCCGGTATCCTGGCAGGt < 1:243615/71‑1 (MQ=255) tcaCCGCCGCCGGTCCACACGCGCTGGCCTTTTTTACCGACGATGATCGCGGTGCCGGTATCCTGGCAGGt > 1:4035952/1‑71 (MQ=255) tcaCCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGa < 1:646429/66‑1 (MQ=255) accaccaccGGTCCATACACGCTGCCCTTTTTTACCAACaataat > 1:4356031/1‑45 (MQ=255) accaccaccGGTCCATACACGCTGCCCTTTTTTACCAACAATAATcg > 1:1572158/1‑47 (MQ=255) ccaccaccGGTCCATACACGCTGCCCTTTTTTACCAACaata < 1:431388/42‑1 (MQ=255) ccaccaccGGTCCATACACGCTGCCCTTTTTTACCAACAATAATc < 1:1340906/45‑1 (MQ=255) ccgccGGTCCACACGCGCTGGCCTTTTTTACCGACGATGATCGCGGTGCCGGTATCCTGGCAGGTCGGCAg < 1:2558444/68‑1 (MQ=255) ccaccGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAg < 1:1060718/71‑1 (MQ=255) caccGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGa < 1:3155127/71‑1 (MQ=255) caccGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGa < 1:304152/71‑1 (MQ=255) ccGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCgg > 1:3911609/1‑49 (MQ=255) ccGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGaa > 1:1963509/1‑70 (MQ=255) ggTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGaa > 1:3296863/1‑68 (MQ=255) ggTCCACACGCGCTGGCCTTTTTTACCGACGATGATcgc < 1:5383109/39‑1 (MQ=9) gTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCgg < 1:2627401/46‑1 (MQ=255) gTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCgg < 1:5573713/46‑1 (MQ=255) gTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGcc > 1:4816617/1‑71 (MQ=255) gTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGc < 1:37240/70‑1 (MQ=255) tCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCgg < 1:4286053/45‑1 (MQ=255) ccATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTa > 1:3679966/1‑46 (MQ=255) ccATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGa > 1:3388531/1‑52 (MQ=255) aTACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAg < 1:1308118/61‑1 (MQ=255) tACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGc < 1:3271662/65‑1 (MQ=255) acgcGCTGGCCTTTTTTACCGACGATGATCGCGGTGCCGGTATCCTGGCAGGTCGGCAGCACGCCTTTggc > 1:3611482/4‑71 (MQ=255) acgcGCTGGCCTTTTTTACCGACGATGATCGCGGTGCCGGTATCCTGGCAGGTCGGCAGCACGCCTTTggc > 1:2231729/4‑71 (MQ=255) acgcGCTGGCCGTTTTTACCGACGATGATCGCGGTGCCGGTATCCTGGCAGGTCGGCAGCACGCCTTTggc > 1:5263149/4‑71 (MQ=255) acacGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGaa > 1:3667765/1‑61 (MQ=255) cacGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCg > 1:3166586/1‑38 (MQ=255) cacGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTcgcc > 1:5557374/1‑71 (MQ=255) gcGCTGGCCTTTTTTACCGACGATGATCGCGGTGCCGGTATCCTGGCAGGTCGGCAGCACGCCtttgg > 1:3773496/2‑66 (MQ=255) cGCTGGCCTTTTTTACCGACGATGATCGCGGTGCCGGTATCCTGGCAGGTCGGCAGCACGCCTTTggcggc < 1:4973483/71‑1 (MQ=255) cGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTcgccgc < 1:1316713/71‑1 (MQ=255) cGCTGCCCTTTTTAACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTcgccgc < 1:1974887/71‑1 (MQ=255) cGCTGCCCTTTTTAACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTcgccgc < 1:1075037/71‑1 (MQ=255) gccTTTTTTACCGACGATGATCGCGGTGCCGGTATCCTGGCAGGTCGGCAGCACGCCTTTggcggc < 1:4051551/65‑1 (MQ=255) ccTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTc < 1:2657598/71‑1 (MQ=255) cTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCt < 1:1546240/56‑1 (MQ=255) ttttttACCGACGATGATCGCGGTGCCGGTATCCTGGCAGGTCGGCAGCACGCCTTTggcggc < 1:4500332/63‑1 (MQ=255) ttttttACCGACGATGATCGCGGTGCCGGTATCCTGGCAGGTCGGCAGCACGCCTTTggcggc < 1:3290668/63‑1 (MQ=255) ttttttACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTcgccgc < 1:3174130/63‑1 (MQ=255) ttttttACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTcgccgc < 1:1661459/63‑1 (MQ=255) ttttttACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGa > 1:4324635/1‑65 (MQ=255) ttttttACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCg > 1:1028860/1‑70 (MQ=255) ttttttACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCg > 1:2546137/1‑70 (MQ=255) ttttttACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCg > 1:150716/1‑70 (MQ=255) tttttACCGACGATGATCGCGGTGCCGGTATCCTGGCAGGTCGGCAGCACGCCTTTggcggc < 1:3672295/62‑1 (MQ=255) tttttACCGACGATGATCGCGGTGCCGGTATCCTGGCAGGTCGGCAGCACGCCTTTGGCGGCGATTTCg > 1:657737/1‑69 (MQ=255) tttttACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCttt > 1:5230059/1‑56 (MQ=255) tttttACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCttt > 1:5224497/1‑56 (MQ=255) tttttACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCttt > 1:5193701/1‑56 (MQ=255) tttttACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCg > 1:2910695/1‑69 (MQ=255) tttttACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCg > 1:4703573/1‑69 (MQ=255) tttttACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCg > 1:4065337/1‑69 (MQ=255) ttttACCGACGATGATCGCGGTGCCGGTATCCTGGCa > 1:3142188/1‑37 (MQ=21) ttttACCGACGATGATCGCGGTGCCGGTATCCTGGCAGGt > 1:4556733/1‑40 (MQ=25) ttttACCGACGATGATCGCGGTGCCGGTATCCTGGCAGGt > 1:1528893/1‑40 (MQ=25) ttttACCGACGATGATCGCGGTGCCGGTATCCTGGCAGGTCGGCAGCACGCCTTTGGCGGCGATTTCGGAg > 1:5225673/1‑71 (MQ=255) ttttACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGc > 1:1806926/1‑44 (MQ=255) tttACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGt > 1:1643407/1‑71 (MQ=255) ttACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGt < 1:2777173/70‑1 (MQ=255) aCCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGa > 1:5517858/1‑59 (MQ=255) aCCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGa > 1:3033088/1‑59 (MQ=255) acaaTAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTcgccgc < 1:2455454/53‑1 (MQ=255) acaaTAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTcgccgc < 1:5245579/53‑1 (MQ=255) acaaTAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTcgccgc < 1:5196834/53‑1 (MQ=255) acaaTAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTcgccgc < 1:1375116/53‑1 (MQ=255) caaTAATCGCGGTGCCGGTATCCTGACAGGTTGGCAg < 1:4250178/37‑1 (MQ=255) caaTAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAgg < 1:1928952/71‑1 (MQ=255) aataatCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGa > 1:3682989/1‑53 (MQ=255) aataatCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGa > 1:3872794/1‑53 (MQ=255) aataatCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGa > 1:3880397/1‑53 (MQ=255) aataatCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAgg > 1:3286898/1‑70 (MQ=255) | ATAGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGG > Exported/1015661‑1015799 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |