Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 218,082 T→C 9.3% I23I (ATT→ATC yafF → conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported218,0820TC 9.3% 618.6 / 37.4 224I23I (ATT→ATCyafFconserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (105/98);  new base C (10/11);  total (115/109)
Fisher's exact test for biased strand distribution p-value = 8.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 240 total aligned reads displayed.

TAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCA  >  Exported/218012‑218152
                                                                      |                                                                      
tAATGAATGAAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACAtt                                                                        >  1:4631755/1‑71 (MQ=255)
 aatgaatgAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCATGGATACGGCACATTg                                                                       >  1:2279801/1‑71 (MQ=255)
  atgaatgaAGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACAtt                                                                        >  1:3275949/1‑69 (MQ=255)
  atgaatgaAGACGACTGCAAAATAAGAAGAGGAAACGCCGCAGAATTATTTTCAGGGATACGGCACATCGc                                                                      >  1:4188913/1‑71 (MQ=255)
       tgaaGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTAtt                                                                  >  1:1028655/1‑70 (MQ=255)
       tgaaGACGACTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTAtt                                                                  >  1:4497363/1‑70 (MQ=255)
       tgaaGACGACCGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTAtt                                                                  >  1:5295270/1‑70 (MQ=255)
             cgacTGCAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATAttt                                                           >  1:2850084/1‑71 (MQ=255)
              gacTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATAtt                                                            <  1:5644398/69‑1 (MQ=255)
                ctgcAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGa                                                        >  1:5377879/4‑71 (MQ=255)
                  gcAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGAc                                                       >  1:1955574/2‑70 (MQ=255)
                   cAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACAtt                                                                        >  1:4291140/1‑52 (MQ=255)
                   cAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACAtt                                                                        >  1:3119386/1‑52 (MQ=255)
                   cAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACg                                                      >  1:3150165/1‑70 (MQ=255)
                   cAAAATAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGAACATTGCTATTAATATTTTGa                                                        >  1:5052829/1‑68 (MQ=255)
                   cAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACg                                                      >  1:2493940/1‑70 (MQ=255)
                    aaaaTAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACg                                                      >  1:5624108/1‑69 (MQ=255)
                    aaaaTAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGaa                                                    <  1:5492118/71‑1 (MQ=255)
                    aaaaTAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGa                                                     <  1:541146/70‑1 (MQ=255)
                      aaTAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAAt                                                   >  1:4466256/1‑70 (MQ=255)
                       aTAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATga                                                 >  1:264528/1‑71 (MQ=255)
                        tAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGaa                                                    >  1:379098/1‑67 (MQ=255)
                        tAAGAAGAGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGaa                                                    >  1:2778962/1‑67 (MQ=255)
                        tAAGAAGAGGAAACGCCGCAGAATTATTTTCAGGGATACGGCACATCGCTATTAATATTTTAACGAATga                                                 >  1:4359183/1‑70 (MQ=255)
                          agaagaGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATgata                                               <  1:222589/70‑3 (MQ=255)
                          agaagaGGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATgat                                                >  1:606327/1‑68 (MQ=255)
                             agagGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAgg                                            <  1:2341494/70‑1 (MQ=255)
                              gagGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAgg                                            <  1:3475987/69‑1 (MQ=255)
                               agGAAATGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTAt                                         <  1:4941815/71‑1 (MQ=255)
                                   aatgcagcaGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTCaa                                     <  1:2613643/68‑1 (MQ=255)
                                   aaCGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTc                                       <  1:1627686/69‑1 (MQ=255)
                                    atgcagcaGAATTATTTTCAGGGAGACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTCAAg                                    >  1:5087274/3‑71 (MQ=255)
                                    aCGCCGCAGAATTATTTTCAGGGATACGGCACATCGCTATTAATATTTTAACg                                                      <  1:4908362/53‑1 (MQ=38)
                                     tgcagcaGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTCAAgg                                   <  1:3695564/70‑1 (MQ=255)
                                     tgcagcaGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTCAAgg                                   <  1:2716167/70‑1 (MQ=255)
                                     cGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAgg                                   >  1:4587884/1‑71 (MQ=255)
                                      gccgcagAATTATTTTCAGGGATACGGCACATCGCTATTAATATTTTAACGAATGATAAGGTATTCAAgg                                   >  1:1937179/4‑70 (MQ=255)
                                      gcagcaGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATaa                                              >  1:136121/1‑59 (MQ=255)
                                      gcagcaGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATaa                                              >  1:2449430/1‑59 (MQ=255)
                                      gcagcaGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATaa                                              >  1:2413925/1‑59 (MQ=255)
                                       ccgcagAATTATTTTCAGGGATACGGCACATCGCTATTAATATTTTAACGAATGATAAGGTATTc                                       <  1:783247/63‑1 (MQ=255)
                                       cagcagAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCa                                 <  1:1886386/71‑1 (MQ=255)
                                        agcagAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGa                                                        >  1:461686/1‑47 (MQ=255)
                                        agcagAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGAc                                                       >  1:3677220/1‑48 (MQ=255)
                                            gAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTg                                                         >  1:3321208/1‑42 (MQ=255)
                                             aaTTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTCAAGGc                                  <  1:3392236/64‑1 (MQ=255)
                                              attattTTCAGGGATACGGCACATTGCTATTAATATTTTg                                                         <  1:2442029/40‑1 (MQ=255)
                                              attattTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTc                                       <  1:1540354/58‑1 (MQ=255)
                                              attattTTCAGGGATACGGCACATCGCTATTAATATTTTAACGAATGATAAGGTAt                                         >  1:2995486/1‑56 (MQ=255)
                                               ttattTTCAGGGATACGGCACATTGCTATTAATATTTTGACGaa                                                    >  1:37247/1‑44 (MQ=255)
                                               ttattTTCAGGGATACGGCACATTGCTATTAATATTTTGACGa                                                     <  1:1650379/43‑1 (MQ=255)
                                               ttattTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTCAAGGCAg                                >  1:3069697/1‑64 (MQ=255)
                                               ttattTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAg                         <  1:2681700/71‑1 (MQ=255)
                                               ttattTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAg                                    <  1:143796/60‑1 (MQ=255)
                                                tattTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTCAAGGCAg                                <  1:931224/63‑1 (MQ=255)
                                                tattTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGa                        >  1:1265587/1‑71 (MQ=255)
                                                 attTTCAGGGATACGGCACATTGCTATTAATAttt                                                           >  1:3568824/1‑35 (MQ=255)
                                                 attTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAAt                                                   >  1:3594913/1‑43 (MQ=255)
                                                 attTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGAc                       >  1:222132/1‑71 (MQ=255)
                                                 attTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGAc                       >  1:4864749/1‑71 (MQ=255)
                                                 attTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGAc                       >  1:4749170/1‑71 (MQ=255)
                                                 attTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGAc                       >  1:4511087/1‑71 (MQ=255)
                                                 attTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGAc                       >  1:4119912/1‑71 (MQ=255)
                                                 attTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGAc                       >  1:1119788/1‑71 (MQ=255)
                                                 attTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGAc                       >  1:4572908/1‑71 (MQ=255)
                                                  ttttCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAgg                                            <  1:1552763/49‑1 (MQ=255)
                                                  ttttCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACg                      <  1:5224436/71‑1 (MQ=255)
                                                  ttttCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACg                      <  1:553899/71‑1 (MQ=255)
                                                  ttttCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAgg                                   <  1:4347141/58‑1 (MQ=255)
                                                  ttttCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAgg                                   <  1:2389882/58‑1 (MQ=255)
                                                    ttCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTa                                          >  1:3741900/1‑49 (MQ=255)
                                                    ttCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTa                                          >  1:4720122/1‑49 (MQ=255)
                                                    ttCAGGGATACGGCACATCGCTATTAATATTTTAACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGt                     <  1:3501800/70‑1 (MQ=255)
                                                         ggATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTc                                       <  1:1076976/47‑1 (MQ=255)
                                                         ggATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATg               <  1:5590357/71‑1 (MQ=255)
                                                         ggATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGa                 >  1:1779932/1‑69 (MQ=255)
                                                          gATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGa                        >  1:4748012/1‑61 (MQ=255)
                                                          gATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATg               >  1:1171826/1‑70 (MQ=255)
                                                          gATACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGc              <  1:5436065/71‑1 (MQ=255)
                                                            tACGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTCAAGGCAGGGt                             <  1:4252661/54‑1 (MQ=255)
                                                            tACGGCACATCGCTATTAATATTTTAACGAATGATAAg                                             <  1:2969754/38‑1 (MQ=37)
                                                             aCGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGa            >  1:5064653/1‑70 (MQ=255)
                                                             aCGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGa            >  1:76636/1‑70 (MQ=255)
                                                             aCGGCACATCGCTATTAATATTTTAACGAATGATAAg                                             >  1:897682/1‑37 (MQ=37)
                                                              cGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTCCAGGc                                  <  1:1737216/47‑1 (MQ=39)
                                                              cGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTCAAGGc                                  <  1:471468/47‑1 (MQ=255)
                                                              cGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTCAAGGc                                  <  1:4791961/47‑1 (MQ=255)
                                                              cGGCACATTGCTATTAATATTTTGACGAATGATAAGGTATTCAAGGc                                  <  1:2285930/47‑1 (MQ=255)
                                                              cGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGaa           >  1:4851396/1‑70 (MQ=255)
                                                              cGGCACATCGCTATTAATATTTTAACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGaa           <  1:1971692/70‑1 (MQ=255)
                                                               ggCACATCGCTATTAATATTTTAACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGaa           >  1:5130413/1‑69 (MQ=255)
                                                                gCACATTGCTATTAATATTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAag        <  1:2306851/71‑1 (MQ=255)
                                                                gCACATTGCTATTAATATTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAag        <  1:88137/71‑1 (MQ=255)
                                                                 cacaTTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAagc       <  1:3666679/71‑1 (MQ=255)
                                                                    aTTGCTATTAATATTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTaa                   <  1:823319/56‑1 (MQ=255)
                                                                    aTTGCTATTAATATTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAagcagc    <  1:517674/71‑1 (MQ=255)
                                                                    aTTGCTATTAATATTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAag        <  1:724639/67‑1 (MQ=255)
                                                                    aTTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGt                     <  1:4618368/54‑1 (MQ=255)
                                                                     ttGCTATTAATATTTTGACGAATGATAAGGTATTCAAGGCa                                 >  1:4018822/1‑41 (MQ=255)
                                                                     ttGCTATTAATATTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAagcagc    >  1:1312504/1‑70 (MQ=255)
                                                                      |                                                                      
TAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCA  >  Exported/218012‑218152

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: