| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,015,835 | C→T | 9.6% | E78E (GAG→GAA) | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,015,835 | 0 | C | T | 9.6% | 201.1 / 9.3 | 73 | E78E (GAG→GAA) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base C (38/28); new base T (2/5); total (40/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.33e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
GAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGA > Exported/1015765‑1015904 | gAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGcc > 1:978119/1‑71 (MQ=255)gAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGcc > 1:321512/1‑71 (MQ=255)gAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGcc > 1:4096225/1‑71 (MQ=255)gAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGcc > 1:4326027/1‑71 (MQ=255) aaCGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCt < 1:2604459/71‑1 (MQ=255) cGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTcc > 1:2402841/1‑71 (MQ=255) cGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTcc > 1:4245253/1‑71 (MQ=255) ccTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTCGTTTTCGCTGGCTTctg > 1:3510957/1‑68 (MQ=255) ttCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGt > 1:2318724/1‑70 (MQ=255) ttCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTACGGGt > 1:5195157/1‑70 (MQ=255) cgcgATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACg > 1:53878/1‑69 (MQ=255) cgcgATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACg > 1:5304385/1‑69 (MQ=255) cgcgATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACg > 1:5350317/1‑69 (MQ=255) cgATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGa < 1:3181149/71‑1 (MQ=255) atttCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTCGTTTTCGCTGGCTTCTGGATCGTGAAGAAt < 1:5408500/68‑1 (MQ=255) tGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCgg > 1:4340196/1‑58 (MQ=255) tGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATg < 1:5305721/71‑1 (MQ=255) gTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGc > 1:3968666/1‑64 (MQ=255) gTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGt > 1:1802020/1‑71 (MQ=255) gTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGt > 1:780844/1‑71 (MQ=255) aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCg > 1:5484388/1‑47 (MQ=255) aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCg > 1:3334255/1‑47 (MQ=255) aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCg > 1:3739970/1‑47 (MQ=255) aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCg > 1:1097490/1‑47 (MQ=255) aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCg > 1:1514323/1‑47 (MQ=255) aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCAcc < 1:429491/70‑1 (MQ=255) aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCAc > 1:1528072/1‑69 (MQ=255) aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCt > 1:5421536/1‑71 (MQ=255) cGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGTTCACGCAGAATg < 1:3467043/60‑1 (MQ=255) cGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAGTg < 1:2447274/60‑1 (MQ=255) cGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATg < 1:4853194/60‑1 (MQ=255) aagAATTGCAGCGCCACGTACTTGTCGTTTTCGCTGGCTTCTGGATCGTGAAGAATAGCCGCAACCtgt < 1:4656579/67‑1 (MQ=255) aGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCtgttg < 1:4675294/71‑1 (MQ=255) ggAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCtgttgt > 1:4422276/1‑71 (MQ=255) ggAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCtgttgt > 1:4551879/1‑71 (MQ=255) gAATTGCAGCGCCACGTACTTGTCGTTTTCGCTGGCTTCTGGATCGTGAAGAATAGCCGCAACCtgtttct < 1:454642/71‑4 (MQ=255) gAATTGCAGCGCCACGTACTTGTCGTTTTCGCTGGCTTCTGGATCGTGAAGAATAGCCGCAACCtgtttct < 1:5373429/71‑4 (MQ=255) gAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTgttgtt < 1:3149309/71‑1 (MQ=255) gAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTgttgtt < 1:1863648/71‑1 (MQ=255) aaTTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGaa > 1:1826152/1‑52 (MQ=255) aaTTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGttgttg < 1:1931121/71‑1 (MQ=255) ttGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGt < 1:1562051/53‑1 (MQ=255) ttGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGt < 1:4871374/53‑1 (MQ=255) ttGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTgttgtt < 1:5392302/68‑1 (MQ=255) gCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTgc > 1:875784/1‑71 (MQ=255) cAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTgcg < 1:396762/71‑1 (MQ=255) aGCGCCACGTACTTGTCGTTTTCGCTGGCTTCTGGATCGTGAAGAATAGCCGCAACCTGTTTCTGGTgtgc < 1:5010150/71‑1 (MQ=255) cacaTATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCtgtt < 1:3193465/56‑1 (MQ=255) acaTATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTgg < 1:4727920/60‑1 (MQ=255) atatTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGcc < 1:4517514/46‑1 (MQ=255) atatTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCtgttgt < 1:5620737/55‑1 (MQ=255) atatTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGt > 1:1850817/1‑59 (MQ=255) atatTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGt > 1:1200501/1‑59 (MQ=255) atatTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTg > 1:2582099/1‑60 (MQ=255) tatTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACgcag > 1:2084705/1‑71 (MQ=255) tatTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACgcag > 1:2288621/1‑71 (MQ=255) tatTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACgcag > 1:4616315/1‑71 (MQ=255) tttATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACgcagc < 1:1409482/70‑1 (MQ=255) tATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCtgttgt > 1:2550882/1‑50 (MQ=255) tCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTgg < 1:677568/51‑1 (MQ=255) tCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGa < 1:4186115/70‑1 (MQ=255) cATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGaa < 1:1095304/70‑1 (MQ=255) cATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAac > 1:5410106/1‑71 (MQ=255) aTTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAac < 1:4840600/70‑1 (MQ=255) ttttCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGt < 1:4804445/49‑1 (MQ=255) ttttCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGt < 1:3106069/49‑1 (MQ=255) ttttCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGt < 1:3915321/49‑1 (MQ=255) tttCGCTGGCTTCTGGATCGTGAAGAATAGCCGCAACCTGTTTCTGGTGTGCCGGGCGGAGCATaaaag > 1:3933684/1‑67 (MQ=255) tttCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAacgac > 1:3686526/1‑71 (MQ=255) ttCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTgc > 1:684587/1‑49 (MQ=255) ttCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGCacgacg > 1:3489365/1‑71 (MQ=255) ttCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAacgacg > 1:3075915/1‑71 (MQ=255) ttCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAacgacg > 1:3470127/1‑71 (MQ=255) tCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCtgt > 1:1443488/1‑39 (MQ=255) tCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAacgacg > 1:5675953/1‑70 (MQ=255) ggCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTgg > 1:1229778/1‑40 (MQ=255) gCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATg < 1:1682144/71‑1 (MQ=255) ccTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGa > 1:3151155/1‑47 (MQ=255) ccTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGa > 1:284304/1‑47 (MQ=255) ccTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGa < 1:1500956/71‑1 (MQ=255) ccTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGa < 1:3651006/71‑1 (MQ=255) ccTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGa < 1:1276736/71‑1 (MQ=255) ccTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGa < 1:3086112/71‑1 (MQ=255) ccTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGa < 1:4063550/71‑1 (MQ=255) ccTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGa < 1:157465/71‑1 (MQ=255) | GAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGA > Exported/1015765‑1015904 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |