Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,036,915 C→G 11.3% A28G (GCC→GGC)  rsxD → predicted inner membrane oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,036,9150CG11.3% 285.1 / 22.2 106A28G (GCC→GGC) rsxDpredicted inner membrane oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (51/43);  new base G (5/7);  total (56/50)
Fisher's exact test for biased strand distribution p-value = 5.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01

Only 100 of 110 total aligned reads displayed.

ATAGCTAGCTCCCCTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTGGGGTACTCTCGTTCAGATCCTGTTGGCATCG  >  Exported/1036845‑1036985
                                                                      |                                                                      
aTAGCTAGCTCCCCTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGc                                                                        >  1:3382317/1‑71 (MQ=255)
       gctCCCCTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGcc                                                                  <  1:1489575/70‑1 (MQ=255)
            ccTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAAt                                                            <  1:1772019/71‑1 (MQ=255)
             cTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGcc                                                                       >  1:5116898/1‑59 (MQ=255)
             cTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGaa                                                             >  1:2678016/1‑69 (MQ=255)
             cTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGaa                                                             >  1:2838546/1‑69 (MQ=255)
                ataCCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCg                                                                      >  1:3631476/1‑57 (MQ=255)
                ataCCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCg                                                                      >  1:385278/1‑57 (MQ=255)
                 taCCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGt                                                                     >  1:3585326/1‑57 (MQ=255)
                 taCCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCg                                                          <  1:1363671/68‑1 (MQ=255)
                 taCCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAg                                                       <  1:3267009/71‑1 (MQ=255)
                  aCCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAgc                                                      >  1:477417/1‑71 (MQ=255)
                   cccATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAgc                                                      <  1:4615925/70‑1 (MQ=255)
                     cATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAgcgc                                                    >  1:5234443/1‑70 (MQ=255)
                     cATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAgcgc                                                    >  1:870967/1‑70 (MQ=255)
                     cATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCa                                                   <  1:2852301/71‑1 (MQ=255)
                      aTAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCaa                                                  <  1:1019901/71‑1 (MQ=255)
                      aTAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCaa                                                  <  1:3368504/71‑1 (MQ=255)
                      aTAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCaa                                                  <  1:2037839/71‑1 (MQ=255)
                       tAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTTCCAGGAATCGc                                                         <  1:4354260/63‑1 (MQ=255)
                       tAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAAt                                                            >  1:2359107/1‑60 (MQ=255)
                       tAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGc                                                         <  1:4024904/63‑1 (MQ=255)
                       tAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAAc                                                 >  1:1540604/1‑71 (MQ=255)
                        aaacAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACt                                                >  1:61651/4‑71 (MQ=255)
                        aaCCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACt                                                >  1:1337783/1‑71 (MQ=255)
                         aCCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGc                                                                   >  1:616715/1‑51 (MQ=255)
                         aCCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGc                                                                   >  1:3572395/1‑51 (MQ=255)
                         aCCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCTAGGAATCGCAGCGCAACt                                                >  1:4335697/1‑70 (MQ=255)
                         aCCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAgg                                                               >  1:2047406/1‑55 (MQ=255)
                         aCCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAg                                                                >  1:701602/1‑54 (MQ=255)
                         aCCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGa                                                              >  1:5127305/1‑56 (MQ=255)
                         aCCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACtg                                               >  1:1428034/1‑71 (MQ=255)
                         aCCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACtg                                               <  1:2262150/71‑1 (MQ=255)
                         aCCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACtg                                               >  1:4161347/1‑71 (MQ=255)
                         aCCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACtg                                               <  1:3781144/71‑1 (MQ=255)
                         aCCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACtg                                               >  1:189542/1‑71 (MQ=255)
                          ccAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATc                                                           <  1:2675933/58‑1 (MQ=255)
                          ccAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATc                                                           <  1:2580222/58‑1 (MQ=255)
                          ccAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCa                                                        >  1:5667701/1‑61 (MQ=255)
                               gcCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAgg                                                               <  1:5571735/49‑1 (MQ=255)
                               gcCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAgg                                                               <  1:3956729/49‑1 (MQ=255)
                               gcCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGttt                                         >  1:5472455/1‑71 (MQ=255)
                                ccAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGaa                                                             <  1:4915510/50‑1 (MQ=255)
                                ccAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGtt                                          <  1:1912598/69‑1 (MQ=255)
                                 cAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGc                                                                   >  1:4997507/1‑43 (MQ=255)
                                  aGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGtttttt                                      <  1:3002321/71‑1 (MQ=255)
                                  aGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGtttttt                                      <  1:3961225/71‑1 (MQ=255)
                                  aGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGtttttt                                      <  1:2370063/71‑1 (MQ=255)
                                  aGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGtttttt                                      <  1:2086494/71‑1 (MQ=255)
                                   gACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTttg                                     <  1:1770833/71‑1 (MQ=255)
                                   gACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTttg                                     <  1:4893702/71‑1 (MQ=255)
                                     cATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTttggt                                   <  1:5177379/71‑1 (MQ=255)
                                     cATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTttggt                                   <  1:3225673/71‑1 (MQ=255)
                                         gcgcATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGttttt                                       >  1:1394471/1‑63 (MQ=255)
                                         gcgcATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGttttt                                       >  1:4407964/1‑63 (MQ=255)
                                          cgcATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTttggt                                   <  1:2497912/66‑1 (MQ=255)
                                                aTGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCaa                                                  >  1:380299/1‑45 (MQ=255)
                                                aTGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCaa                                                  >  1:5158545/1‑45 (MQ=255)
                                                aTGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCaa                                                  >  1:449340/1‑45 (MQ=255)
                                                aTGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTttg                                     >  1:906235/1‑58 (MQ=255)
                                                aTGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTGGGGTActc                         >  1:4823916/1‑70 (MQ=255)
                                                aTGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTGGGGTActc                         <  1:2946173/70‑1 (MQ=255)
                                                  gCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTttggttg                                 <  1:466619/60‑1 (MQ=255)
                                                   cTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGtttt                                        >  1:904874/1‑52 (MQ=255)
                                                   cTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTGGGGTACTCTCg                      <  1:3341867/70‑1 (MQ=255)
                                                      ttGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTGGGGTACTCTCGtt                    <  1:3644329/69‑1 (MQ=255)
                                                       tGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGtttt                                        >  1:3013343/1‑48 (MQ=255)
                                                       tGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGtttt                                        >  1:130791/1‑48 (MQ=255)
                                                       tGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGtttt                                        >  1:2024024/1‑48 (MQ=255)
                                                         gtgtTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTGGGGTACTCTCGtt                    >  1:4007974/1‑66 (MQ=255)
                                                           gtTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGtt                                          <  1:1056520/42‑1 (MQ=255)
                                                           attgCTCGCAGGCGTGCCgtttgccatacacattgaaagacgatc                                       <  1:4407867‑M1/44‑28 (MQ=255)
                                                           attgCTCGCAGGCGTGCCgtttgccatacacattgaaagacgatc                                       <  1:83254‑M1/44‑28 (MQ=255)
                                                           attgCTCGCAGGCGTGCCgtttgccatacacattgaaagacgatc                                       <  1:1634870‑M1/44‑28 (MQ=255)
                                                            ttGCTCGCAGGCGTGCCttttgccatacacattgaaagacga                                         <  1:1091202‑M1/42‑26 (MQ=255)
                                                            ttGCTCGCAGGCGTGCCgtttgccatacacattgaaagacga                                         <  1:802490‑M1/42‑26 (MQ=255)
                                                            ttGCTCGCAGGCGTGCCgtttgccatacacattgaaaga                                            >  1:3475854‑M1/1‑17 (MQ=255)
                                                            ttGCTCGCAGGCGTGCCgtttgccatacacattgaaaga                                            >  1:82173‑M1/1‑17 (MQ=255)
                                                            ttGCTCGCAGGCGTGCCgtttgccatacacattgaaaga                                            >  1:1614225‑M1/1‑17 (MQ=255)
                                                            ttGCTCGCAGGCGTGCCgtttgccatacacattgaaag                                             >  1:2396428‑M1/1‑17 (MQ=255)
                                                             tGCTCGCAGGCGTGCCgtttgccatacacattgaaagacgatc                                       >  1:4957110‑M1/1‑16 (MQ=255)
                                                             tGCTCGCAGGCGTGCCgtttgccatacacattgaaa                                              <  1:610549‑M1/36‑21 (MQ=255)
                                                             tGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTGGGGTACTCTCGTTCAGATCCTg           >  1:410635/1‑71 (MQ=255)
                                                             tGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTGGGGTACTCTCGTTCAGATCCTg           >  1:1638132/1‑71 (MQ=255)
                                                              gCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTGGGGTACTCTCg                      >  1:934218/1‑59 (MQ=255)
                                                                tCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTGGGGTACTCTCGTTCAGATCCTg           <  1:1829806/68‑1 (MQ=255)
                                                                 cGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTttggttg                                 >  1:4851937/1‑45 (MQ=255)
                                                                 cGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTGGGGTACTCTCGTTCAGATCCTGTTgg       >  1:845769/1‑71 (MQ=255)
                                                                 cGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTGGGGTACTCTCGTTCAGATCCTGTTg        >  1:1208721/1‑70 (MQ=255)
                                                                  gCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTGGGGTACTCTCGTTCAGATCCTGTTg        <  1:5227757/69‑1 (MQ=255)
                                                                  gCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTGGGGTACTCTCGTTCAGATCCTGTTGGc      <  1:2490813/71‑1 (MQ=255)
                                                                   cggCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTGGGGTACTCTCGTTCAGa                >  1:2412435/3‑60 (MQ=255)
                                                                   cAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTGGGGTACTCTCGTTCAGATCCTGTTg        >  1:2338333/1‑68 (MQ=255)
                                                                   cAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTGGGGTACTCTCGTTCAGATCCTGTTGGCa     <  1:1120512/71‑1 (MQ=255)
                                                                   cAGCCGTGCCAGGAATCGCAGCGCAACTGTGGGTTTTTGGTTGGGGTACTCTCGTTCAGAACCTGTTg        >  1:1605485/1‑68 (MQ=255)
                                                                    aGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTGGGGTACTCTCGTTc                   >  1:1452164/1‑56 (MQ=255)
                                                                    aGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTGGGGTACTCTCGTTCAGATCCTGTTGGCAt    <  1:5479527/71‑1 (MQ=255)
                                                                     gCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTGGGGTACTCTCGTTCAGATCCTGTTGGCATc   <  1:4295419/71‑1 (MQ=255)
                                                                      ccGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTGGGGTACTCTCGTTCAGATCCTGTTGGCATc   <  1:753031/70‑1 (MQ=255)
                                                                      ccGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTGGGGTACTCTCGTTCAGATCCTGTTGGCATCg  <  1:4871551/71‑1 (MQ=255)
                                                                      |                                                                      
ATAGCTAGCTCCCCTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTGGGGTACTCTCGTTCAGATCCTGTTGGCATCG  >  Exported/1036845‑1036985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: