Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,191,074 A→C 78.2% intergenic (+415/+485) yeaJ → / ← rnd predicted diguanylate cyclase/ribonuclease D

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,191,0740AC78.2% 116.6 / 17.4 50intergenic (+415/+485)yeaJ/rndpredicted diguanylate cyclase/ribonuclease D
Reads supporting (aligned to +/- strand):  ref base A (5/6);  new base C (16/23);  total (21/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTA  >  Exported/1191010‑1191144
                                                                |                                                                      
tGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAgcgc                                                                   >  1:5570332/1‑70 (MQ=255)
tGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCt                                                                  <  1:4673634/71‑1 (MQ=255)
tGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCt                                                                  <  1:2088420/71‑1 (MQ=255)
tGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCAGGCAATGTCTGCGCTTTTTCCATCAgcgc                                                                   >  1:1267023/1‑70 (MQ=255)
  tCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTag                                                                >  1:5608914/1‑71 (MQ=255)
    cATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTagc                                                               <  1:4215690/70‑1 (MQ=255)
    cATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTag                                                                >  1:3496956/1‑69 (MQ=255)
    cATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTag                                                                >  1:5306911/1‑69 (MQ=255)
      tCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTagcagc                                                            <  1:419628/71‑1 (MQ=255)
      tCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTagcagc                                                            <  1:5386794/71‑1 (MQ=255)
        aGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGt                                                          >  1:4852254/1‑71 (MQ=255)
            tAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTag                                                                >  1:787049/1‑61 (MQ=255)
            tAAGCATCGGCTACGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTa                                                      >  1:4621321/1‑71 (MQ=255)
                 aTCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGt                                                  >  1:42101/1‑70 (MQ=255)
                    ggCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGa                                                >  1:157306/1‑69 (MQ=255)
                         cGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCg                                           <  1:5407908/69‑1 (MQ=255)
                            tAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTa                                                                 <  1:3120347/44‑1 (MQ=255)
                            tAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTa                                                                 <  1:1226220/44‑1 (MQ=255)
                            tAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGAttt                                      >  1:4164948/1‑71 (MQ=255)
                            tAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGAtt                                       >  1:5564887/1‑70 (MQ=255)
                              aGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCg                                    >  1:1094284/1‑71 (MQ=255)
                              aGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCg                                    >  1:3610064/1‑71 (MQ=255)
                               ggCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGc                                   <  1:2876094/71‑1 (MQ=255)
                                gCATCTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCt                                  <  1:1975552/71‑1 (MQ=255)
                                 cATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTa                                 <  1:5551022/71‑1 (MQ=255)
                                  aTCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTa                                 <  1:4915245/70‑1 (MQ=255)
                                  aTCTTCCGGCAATGTCTGAGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTAc                                >  1:2068519/1‑71 (MQ=255)
                                    cTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCg                                           <  1:4176068/58‑1 (MQ=255)
                                    cTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCg                                           <  1:5471537/58‑1 (MQ=255)
                                    cTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTc                                     <  1:573205/64‑1 (MQ=255)
                                    cTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTc                                     <  1:4076684/64‑1 (MQ=255)
                                    cTTCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCg                              >  1:1647132/1‑71 (MQ=255)
                                     ttCCGGCAATGTCTGCGCTTTTTCCACCAGCGCTa                                                                 >  1:3476348/1‑35 (MQ=255)
                                       ccGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTAcc                               <  1:1848379/67‑1 (MQ=255)
                                       ccGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGa                            <  1:1000166/70‑1 (MQ=255)
                                       ccGGCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGa                            <  1:537302/70‑1 (MQ=255)
                                        cGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATa                          <  1:553018/71‑1 (MQ=255)
                                         ggCAATGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATaa                         <  1:865928/71‑1 (MQ=255)
                                            aaTGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTc                                     <  1:1859781/56‑1 (MQ=255)
                                              tGTCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGAtt                                       <  1:2956484/52‑1 (MQ=255)
                                                tCTGCGCTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAg                   <  1:4070332/70‑1 (MQ=255)
                                                    cgcTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTg               <  1:2098382/70‑1 (MQ=255)
                                                     gcTTTTTCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTc             >  1:4241315/1‑71 (MQ=255)
                                                       tttttCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCgg                                          <  1:1532951/40‑1 (MQ=255)
                                                       tttttCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCgg                                          <  1:1113457/40‑1 (MQ=255)
                                                       tttttCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCgg                                          <  1:86608/40‑1 (MQ=255)
                                                         tttCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGt         >  1:1646456/1‑71 (MQ=255)
                                                          ttCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAg          <  1:3671557/69‑1 (MQ=255)
                                                          ttCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGc                                   >  1:1228504/1‑44 (MQ=255)
                                                           tCCACCAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAAccc                     >  1:207282/1‑57 (MQ=255)
                                                            ccaccaGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTc       <  1:665912/70‑1 (MQ=255)
                                                                aaGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTa  <  1:2564804/71‑1 (MQ=255)
                                                                |                                                                      
TGTCCATCAGGTTAAGCATCGGCTGCGGTAAGGCATCTTCCGGCAATGTCTGCGCTTTTTCCACAAGCGCTAGCAGCGTTTTACCGTGAAAGCGGATTTCGCTACCGGATAAACCCAGGCTGTCCAGTTCGCCTA  >  Exported/1191010‑1191144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: