| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 218,157 | C→T | 22.8% | D48D (GAC→GAT) | yafF → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 218,157 | 0 | C | T | 22.8% | 305.2 / 84.2 | 162 | D48D (GAC→GAT) | yafF | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (67/58); new base T (18/19); total (85/77) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.08e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01 | |||||||||||
Only 100 of 164 total aligned reads displayed.
AATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTAATACCCCGACTCTCCCCGT > Exported/218089‑218223 | aaTATTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACa < 1:5304034/70‑1 (MQ=255) tatTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGa < 1:3503476/70‑1 (MQ=255) tatTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGa > 1:2251442/1‑70 (MQ=255) tatTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGa < 1:4680091/70‑1 (MQ=255) tatTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGa > 1:4715980/1‑70 (MQ=255) tatTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGa < 1:5140282/70‑1 (MQ=255) tatTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGa < 1:1887636/70‑1 (MQ=255) tatTTTGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGa < 1:3470379/70‑1 (MQ=255) tatTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGa < 1:2102764/70‑1 (MQ=255) ttttGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGaa < 1:3282201/69‑1 (MQ=255) ttttGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGaa < 1:1509462/69‑1 (MQ=255) ttttGACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAAc > 1:5523771/1‑71 (MQ=255) ttttAACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGaa < 1:2770489/69‑1 (MQ=255) ttaaCGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACt < 1:2379544/67‑1 (MQ=255) gACGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACa < 1:4241833/62‑1 (MQ=255) aCGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCt < 1:4838807/71‑1 (MQ=255) tGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTATCt < 1:5416401/66‑1 (MQ=255) ataaGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGATAAGCAGCCATGGACAGaa > 1:17552/3‑57 (MQ=255) ataaGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGaaa > 1:5087652/3‑58 (MQ=255) ataaGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGaaa > 1:4960252/3‑58 (MQ=255) ataaGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGaa > 1:3927575/3‑57 (MQ=255) ataaGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTATCTGGCGTc < 1:3974561/68‑1 (MQ=255) ataaGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGt > 1:3304418/3‑69 (MQ=255) taaGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAg > 1:5249375/2‑71 (MQ=255) aaGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGa < 1:2392675/54‑1 (MQ=255) aaGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAg > 1:3483338/1‑70 (MQ=255) aGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGt < 1:4680723/70‑1 (MQ=255) ggTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTATCTGGCGTCAGTcc < 1:3881365/71‑1 (MQ=255) gTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCt < 1:3867706/71‑1 (MQ=255) tATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCtt < 1:2451934/71‑1 (MQ=255) ttCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACt < 1:2998032/52‑1 (MQ=255) ttCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACt < 1:4926133/52‑1 (MQ=255) ttCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTATTTGGCGTCAGTCCTTGc < 1:1664669/71‑1 (MQ=255) ttCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAg < 1:531635/64‑1 (MQ=255) ttCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAg < 1:1008257/64‑1 (MQ=255) ttCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCtta < 1:1008818/70‑2 (MQ=255) tCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCg > 1:5050505/1‑71 (MQ=255) cAAGGCATGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTACgg > 1:2166309/1‑71 (MQ=255) cAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCgg > 1:4845463/1‑71 (MQ=255) cAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTATCTGGCGTCAGTCCTTGCgg > 1:792673/1‑71 (MQ=255) cAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCa > 1:1116609/1‑61 (MQ=255) cAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCa > 1:3382194/1‑61 (MQ=255) cAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCttac > 1:5580842/1‑67 (MQ=255) cAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCgg > 1:3109827/1‑71 (MQ=255) cAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCgg > 1:2598466/1‑71 (MQ=255) cAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCgg > 1:380270/1‑71 (MQ=255) aaGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAAc > 1:3521461/1‑48 (MQ=255) aaGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTATCTGGCGTCAGTCCTTGCgg < 1:5237182/70‑1 (MQ=255) aaGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTACgg > 1:2833266/1‑70 (MQ=255) ggCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTACGGGGa < 1:4380476/71‑1 (MQ=255) aGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGaaa > 1:2302272/1‑42 (MQ=255) gggTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCgg > 1:3965276/1‑71 (MQ=255) gTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCgggg < 1:5216980/64‑1 (MQ=255) ttAAGACGTAAGATGCGAAAAGCAGCCATGGACAGaaa > 1:3265805/1‑38 (MQ=255) ttAAGACGTAAGATGCGAAAAGCAGCCATGGACAGaaa > 1:2721919/1‑38 (MQ=255) ttAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTATCTGGCGTCAGTCCTTGCGGGGAGCGGGCt > 1:3987943/1‑71 (MQ=255) aaGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCa > 1:4586445/1‑49 (MQ=255) aaGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCttt > 1:899542/1‑71 (MQ=255) gACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTCg < 1:1388404/71‑1 (MQ=255) cGTAAGATGCGAAAAGCAGCCATGGACAGAAACTATCTGGCGTCAGTCCTTGc > 1:745403/1‑53 (MQ=255) cGTAAGATGCGAAAAGCAGCCATGGACAGAAACTATCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCgta < 1:2202383/71‑1 (MQ=255) cGTAAGATGCGAAAAGCAGCCATGGACAGAAACTATCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCgta < 1:1786494/71‑1 (MQ=255) gTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGt > 1:2020530/1‑42 (MQ=255) tAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCg < 1:664308/52‑1 (MQ=255) tAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCgtag > 1:1978372/1‑70 (MQ=255) tAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTACgggg < 1:3460643/55‑1 (MQ=255) aaGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTc > 1:2716047/1‑65 (MQ=255) aaGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGAGGGGAGCGGGCTTTc > 1:4938711/1‑65 (MQ=255) gATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCa < 1:5604824/40‑1 (MQ=255) gATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCa < 1:1311927/40‑1 (MQ=255) gATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTc < 1:3547129/43‑1 (MQ=255) gATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTc < 1:2275215/43‑1 (MQ=255) gATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTc < 1:5034668/43‑1 (MQ=255) gATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTc < 1:1664192/43‑1 (MQ=255) gATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCa > 1:5440118/1‑40 (MQ=255) gATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCa > 1:2913806/1‑40 (MQ=255) gATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCa > 1:4168556/1‑40 (MQ=255) gATGCGAAAAGCAGCCATCGACAGAAACTACCTGGCGTc > 1:1177256/1‑39 (MQ=255) aTGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTACg < 1:2830334/48‑1 (MQ=255) gAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGt > 1:2245327/1‑37 (MQ=37) gAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGt > 1:3451041/1‑37 (MQ=37) gAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGt > 1:3422703/1‑37 (MQ=37) gAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGt > 1:3020164/1‑37 (MQ=37) gAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCgtagta > 1:654207/1‑64 (MQ=255) aaaaGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCgg > 1:3709748/1‑44 (MQ=255) aaaaGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCgg > 1:3008033/1‑44 (MQ=255) aaaaGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCgg > 1:3215063/1‑44 (MQ=255) aaGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCggg < 1:4944522/43‑1 (MQ=255) aaGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCgg > 1:640366/1‑42 (MQ=255) aaGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTACGGGGAGCg > 1:1994317/1‑48 (MQ=255) aaGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTACGGGGAGCGGGCtt < 1:418260/53‑1 (MQ=255) gcagcCATGGACAGAAACTACCTGGCGTCAGTCCTTGCg < 1:4232697/39‑1 (MQ=255) gcagcCATGGACAGAAACTACCTGGCGTCAGTCCTTACGGGGAGCGGGCTTTc > 1:454470/1‑53 (MQ=255) ccATGGACAGAAACTATCTGGCGTCAGTCCTTGCgg < 1:1481279/36‑1 (MQ=255) tGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTCgtaa < 1:1025200/50‑2 (MQ=255) gACAGAAACTACCTGGCGTCAGTCCTTACGGGGAg > 1:5442247/1‑35 (MQ=255) aCAGAAACTATCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCgtaa > 1:588114/1‑46 (MQ=255) aCAGAAACTACCTGGCGTCAGTCCTTACGGGGAGCGGGCTTTCgtaat < 1:1560499/48‑3 (MQ=39) cAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCttt > 1:4446192/1‑41 (MQ=255) cAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTAATACCCCGAct > 1:1316173/1‑59 (MQ=255) | AATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTAATACCCCGACTCTCCCCGT > Exported/218089‑218223 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |