BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	1,201,072	T→G	15.2%	P75P (CCT→CCG)	sdaA →	L‑serine deaminase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	1,201,072	0	T	G	15.2%	225.4 / 42.6	112	P75P (CCT→CCG)	sdaA	L‑serine deaminase I
Reads supporting (aligned to +/- strand): ref base T (50/45); new base G (6/11); total (56/56)
Fisher's exact test for biased strand distribution p-value = 2.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 115 total aligned reads displayed.

CACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAAGAGCGCGAACGTCTGCTGCT  >  Exported/1201002‑1201140

cACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCt                                                                      >  1:1876963/1‑71 (MQ=255)

 aCTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCt                                                                      <  1:106621/70‑1 (MQ=255)

 aCTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTg                                                                     >  1:4793423/1‑71 (MQ=255)

   tGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGc                                                                    >  1:5175328/1‑70 (MQ=255)

   tGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGc                                                                    >  1:2059994/1‑70 (MQ=255)

   tGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGc                                                                    >  1:5539730/1‑70 (MQ=255)

   tGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGc                                                                    >  1:852152/1‑70 (MQ=255)

     tCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCAc                                                                 >  1:1145933/1‑71 (MQ=255)

         tGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTg                                                             >  1:2724003/1‑71 (MQ=255)

           aCGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCAACCGTg                                                             <  1:2093796/69‑1 (MQ=255)

              ggTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGatat                                                        >  1:5640380/1‑71 (MQ=255)

               gTAAAGGCCACCACACTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGatat                                                        >  1:16107/1‑70 (MQ=255)

               gTAAAGGCCACCACACTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATc                                                       <  1:3545152/71‑1 (MQ=255)

                 aaaGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGata                                                         >  1:2191146/1‑67 (MQ=255)

                  aaGGCCACCACACTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATCGa                                                     >  1:4609480/1‑70 (MQ=255)

                  aaGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGa                                                     <  1:3802516/70‑1 (MQ=255)

                  aaGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGa                                                     <  1:5313696/70‑1 (MQ=255)

                   aGGCCACCACACTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATCGatt                                                   <  1:1721752/71‑3 (MQ=255)

                    ggCCACCACACTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATCGattc                                                  <  1:2724485/71‑4 (MQ=255)

                    ggCCACCACACTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATCGattc                                                  <  1:822517/71‑4 (MQ=255)

                    ggCCACCACACTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATCGattc                                                  <  1:3683581/71‑4 (MQ=255)

                     gCCACCACACTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATCGattc                                                  >  1:3360804/1‑67 (MQ=255)

                     gCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGa                                                           >  1:1748441/1‑61 (MQ=255)

                      ccaccaCACTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATCGatt                                                   <  1:5359276/68‑3 (MQ=255)

                      ccaccaCACCGATATCGCCATTTTTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTa                                                >  1:5507829/1‑71 (MQ=255)

                         ccacACTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATCGattc                                                  <  1:5044630/66‑4 (MQ=255)

                         ccacACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTg                                                                     <  1:1413433/47‑1 (MQ=255)

                          cacaCTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATc                                                       <  1:4232881/60‑1 (MQ=255)

                           acacCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGa                                                           >  1:1579627/1‑55 (MQ=255)

                            cacCGATATCGCCATTATTATGGTTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCg                                          >  1:688421/1‑71 (MQ=255)

                            cacCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCg                                          >  1:4176217/1‑71 (MQ=255)

                            cacCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCg                                          >  1:1683737/1‑71 (MQ=255)

                             acCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCgg                                         >  1:5515268/1‑71 (MQ=255)

                              ccGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGt                                        >  1:1125878/1‑71 (MQ=255)

                              ccGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGt                                        >  1:3155375/1‑71 (MQ=255)

                               tgATATCGCCATTATTATGGGCCTTGCGGGTAACCTGCCGGATACCGTGGATATCGat                                                    <  1:2910886/57‑2 (MQ=255)

                                 atatCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGAc                                                    <  1:2376697/56‑1 (MQ=255)

                                 atatCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGAc                                                    <  1:5570109/56‑1 (MQ=255)

                                 atatCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGttt                                      >  1:4485513/1‑70 (MQ=255)

                                  tatCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTa                                    <  1:5695205/71‑1 (MQ=255)

                                  tatCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTa                                    <  1:2652223/71‑1 (MQ=255)

                                  tatCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTa                                    <  1:14276/71‑1 (MQ=255)

                                   atCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCAc                                                                 >  1:5268274/1‑41 (MQ=255)

                                   atCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTAt                                   >  1:2619947/1‑71 (MQ=255)

                                    tCGCCATTATTATGGGTCTTGCAGGTAAGGAACCTGCCACCGTGGatat                                                        >  1:8664/1‑49 (MQ=255)

                                    tCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCg                                                               >  1:799848/1‑42 (MQ=255)

                                    tCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCg                                                               >  1:181865/1‑42 (MQ=255)

                                    tCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGatat                                                        >  1:3658793/1‑49 (MQ=255)

                                    tCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTAtt                                  >  1:4179570/1‑71 (MQ=255)

                                    tCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTAt                                   <  1:5274320/70‑1 (MQ=255)

                                    tCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTAt                                   <  1:1733972/70‑1 (MQ=255)

                                    tCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATCGATTCCATCCCCAGTTTTAt                                   >  1:2231524/1‑70 (MQ=255)

                                     cGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTa                                                >  1:42484/1‑56 (MQ=255)

                                       ccATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGt                                                              <  1:334685/40‑1 (MQ=255)

                                       ccATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTcgc                               <  1:438116/71‑1 (MQ=255)

                                        cATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAg                                                  <  1:4998195/51‑1 (MQ=255)

                                        cATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAg                                                  <  1:166450/51‑1 (MQ=255)

                                        cATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAg                                                  <  1:3332616/51‑1 (MQ=255)

                                        cATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAg                                                  <  1:712724/51‑1 (MQ=255)

                                        cATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAg                                                  <  1:3015882/51‑1 (MQ=255)

                                        cATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTcgcg                              <  1:4439293/71‑1 (MQ=255)

                                        cATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTcgc                               >  1:458109/1‑70 (MQ=255)

                                         attattATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAg                                                  <  1:4008774/50‑1 (MQ=255)

                                         attattATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAg                                                  <  1:5573431/50‑1 (MQ=255)

                                         attattATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGa                             >  1:3070030/1‑71 (MQ=255)

                                           tattatGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACg                           >  1:5009563/1‑71 (MQ=255)

                                           tattatGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATCGATTCCATCCCCAGTTTTAt                                   <  1:918092/63‑1 (MQ=255)

                                            attatGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATCGATTCCATCCCCAGTTTTATTc                                 >  1:2963194/1‑64 (MQ=255)

                                             ttatGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTa                         <  1:4607497/71‑1 (MQ=255)

                                               atGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTag                        <  1:1330065/70‑1 (MQ=255)

                                               atGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTag                        <  1:5295182/70‑1 (MQ=255)

                                                tGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTagaa                      <  1:2686352/71‑1 (MQ=255)

                                                  ggTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTagaaga                    <  1:5659484/71‑1 (MQ=255)

                                                    tCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAAGAgc                  <  1:1277218/71‑1 (MQ=255)

                                                    tCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAAGAgc                  <  1:5406685/71‑1 (MQ=255)

                                                        gCAGGTAACGAACCTGCGACCGTGGATATCGACAGTATTCCCGGTTTTATTc                                 >  1:2844949/1‑52 (MQ=255)

                                                        gCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTAtt                                  >  1:4062546/1‑51 (MQ=255)

                                                        gCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTc                                 >  1:3193066/1‑52 (MQ=255)

                                                        gCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTc                                 >  1:1314832/1‑52 (MQ=255)

                                                        gCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAAGAGcgcg               <  1:3789326/70‑1 (MQ=255)

                                                        gCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAAGAGcgcg               <  1:3664178/70‑1 (MQ=255)

                                                           ggTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAAGAGCGCGAACg           <  1:4591619/71‑1 (MQ=255)

                                                            gTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTagaa                      <  1:3319912/59‑1 (MQ=255)

                                                            gTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAAGAGCGCGAACg           <  1:3729720/70‑1 (MQ=255)

                                                            gTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAAGAGCGCGAACGt          <  1:83741/71‑1 (MQ=255)

                                                            gTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAAGAGCGCGAACGt          <  1:1867898/71‑1 (MQ=255)

                                                            gTAACGAACATGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAAGAGCGCGAACGt          >  1:4708744/1‑71 (MQ=255)

                                                             tAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAAGAGcgcg               <  1:2144097/65‑1 (MQ=255)

                                                              aaCGAACCTGCCACCGTGGATATCGACAGTATTCCCGGtt                                       >  1:2542514/1‑40 (MQ=255)

                                                              aaCGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAAGAgcgc                >  1:2158501/1‑63 (MQ=255)

                                                              aaCGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAAGAgcgc                >  1:5675277/1‑63 (MQ=255)

                                                               aCGAACCTGCCACCGTGGATATCGACAGTATTCCCGGttt                                      <  1:608601/40‑1 (MQ=255)

                                                               aCGAACCTGCCACCGTGGATATCGACAGTATTCCCGGttt                                      <  1:5580462/40‑1 (MQ=255)

                                                               aCGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAAGAGCGCGAAc            >  1:1699940/1‑66 (MQ=255)

                                                               aCGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAAGAGCGCGAAc            >  1:2893734/1‑66 (MQ=255)

                                                                cGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTagaa                      >  1:543348/1‑55 (MQ=255)

                                                                  aaCCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAAGAGCGCGAACGTctgctg    >  1:4854142/1‑71 (MQ=255)

                                                                  aaCCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAAGAGCGCGAACGTctgatg    >  1:1298047/1‑71 (MQ=255)

                                                                    ccTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAAGAGCGCGAACGTctgctgct  <  1:2169312/71‑1 (MQ=255)

                                                                     cTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAAGAgc                  <  1:2971960/54‑1 (MQ=255)

CACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAAGAGCGCGAACGTCTGCTGCT  >  Exported/1201002‑1201140

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`