Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | Exported | 1,211,229 | G→A | 15.3% | F41F (TTC→TTT) | cspC ← | stress protein, member of the CspA‑family |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | Exported | 1,211,229 | 0 | G | A | 15.3% | 335.6 / 41.0 | 137 | F41F (TTC→TTT) | cspC | stress protein, member of the CspA‑family |
Reads supporting (aligned to +/- strand): ref base G (64/52); new base A (3/18); total (67/70) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 6.36e-04 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 142 total aligned reads displayed.
AACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAG > Exported/1211160‑1211298 | aaCAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTg < 1:100053/70‑1 (MQ=255) aaCAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTg < 1:2518423/70‑1 (MQ=255) aGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGc < 1:2106075/71‑1 (MQ=255) gCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGc > 1:2169202/1‑70 (MQ=255) tGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGa > 1:785200/1‑65 (MQ=255) gCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCAt > 1:4532151/1‑70 (MQ=255) ccGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTa < 1:1369501/71‑1 (MQ=255) ccGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTa > 1:3423870/1‑71 (MQ=255) ccTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTg < 1:773513/71‑1 (MQ=255) ccTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTg < 1:575558/71‑1 (MQ=255) cTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTAc > 1:98333/1‑66 (MQ=255) tCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGaa > 1:935144/1‑55 (MQ=255) tCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGa > 1:626907/1‑69 (MQ=255) tCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGa > 1:2660368/1‑69 (MQ=255) cTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGaa > 1:3414663/1‑54 (MQ=255) cTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCa > 1:2554025/1‑58 (MQ=255) gCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCATCCAGAGTTTTGAAGCCATTACCCTGGATAGc < 1:4264330/69‑1 (MQ=255) gCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCg < 1:1299178/70‑1 (MQ=255) cGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGgag < 1:3148808/71‑1 (MQ=255) gTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGa > 1:63875/1‑62 (MQ=255) gTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGgag > 1:3579978/1‑70 (MQ=255) gTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATAACCCTGGa > 1:321761/1‑62 (MQ=255) tCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTg < 1:1983637/45‑1 (MQ=255) tCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGc < 1:181383/49‑1 (MQ=255) tCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGga > 1:1729316/1‑68 (MQ=255) ccTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTg < 1:3484238/44‑1 (MQ=255) ccTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAg > 1:2486325/1‑71 (MQ=255) cTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGaa > 1:2539915/1‑45 (MQ=255) cTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGaa > 1:2750985/1‑45 (MQ=255) cTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGaa > 1:5085462/1‑45 (MQ=255) cTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGaa > 1:5601381/1‑45 (MQ=255) cTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGaa > 1:1543151/1‑45 (MQ=255) cTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGaa > 1:2168864/1‑45 (MQ=255) cTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTg < 1:1263722/57‑1 (MQ=255) gtgaTTTCGAACTCTACGCGCTGACCTTCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAgtg < 1:3133154/68‑1 (MQ=255) aaTTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTa > 1:4811216/1‑49 (MQ=255) aaTTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTa < 1:5534542/71‑1 (MQ=255) aaTTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTa < 1:1780813/71‑1 (MQ=255) aaTTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTa > 1:5393203/1‑71 (MQ=255) aTTTCGAACTCTACGCGCTGACCTTCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTAc > 1:1514981/1‑71 (MQ=255) aTTTCGAACTCTACGCGCTGACCTTCAGCAAGAGTTTCAAAACCATTAGTCTGGATTGCAGAGAAgtg < 1:1848828/68‑1 (MQ=255) aTTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTa < 1:5208823/70‑1 (MQ=255) aTTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTAc > 1:3218852/1‑71 (MQ=255) tttCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTAc < 1:3847426/48‑1 (MQ=255) tttCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACg < 1:367860/71‑1 (MQ=255) tttCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACg < 1:2215967/71‑1 (MQ=255) tttCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACg < 1:2343692/71‑1 (MQ=255) tCGAACTCTACGCGCTGACCTTCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGa < 1:2607437/70‑1 (MQ=255) tCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTAcc > 1:2481993/1‑47 (MQ=255) aaCTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAgtg < 1:4910506/62‑1 (MQ=255) aaCTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAgtg < 1:2621380/62‑1 (MQ=255) aaCTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAaca > 1:202511/1‑70 (MQ=255) aCTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTAcc > 1:3794890/1‑43 (MQ=255) aCTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTAcc > 1:5322828/1‑43 (MQ=255) cTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGc < 1:5246201/35‑1 (MQ=255) aaCGTTCTGACCTTCAGCCAGAGTTTTGAAGCCAt > 1:530325/1‑35 (MQ=255) aaCGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCtt > 1:4738548/1‑71 (MQ=255) aCGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGgag < 1:4306100/51‑1 (MQ=255) ctttCTGACCTTCGTCCAGAGATTTGTAACCATCGTTCTGGATAGCAGAGAAgt > 1:3540719/3‑54 (MQ=255) cGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTAcc > 1:5516915/1‑37 (MQ=255) cGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTAcc > 1:1834766/1‑37 (MQ=255) cGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTAcc > 1:148853/1‑37 (MQ=255) cGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTtg < 1:1692410/71‑1 (MQ=255) tttCTGACCTTCGTCCAGAGATTTGTAACCATCGTTCTGGATAGCAgaga < 1:3311892/49‑1 (MQ=255) gTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTAcc > 1:2607791/1‑36 (MQ=255) tCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTtgctg > 1:5603969/1‑71 (MQ=255) tCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTtgctg > 1:4519688/1‑71 (MQ=255) cTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTtgctgc > 1:1254573/1‑71 (MQ=255) tGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGAt < 1:5508843/38‑1 (MQ=255) tGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTtgctgc > 1:468109/1‑70 (MQ=255) gACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCa > 1:5551196/1‑71 (MQ=255) ttCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTa < 1:138043/47‑1 (MQ=255) tCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGc > 1:3933927/1‑71 (MQ=255) tCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGc > 1:2276753/1‑71 (MQ=255) tCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCtt < 1:3821948/58‑1 (MQ=255) tCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCtt < 1:5328213/58‑1 (MQ=255) cAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGgaga > 1:1257439/1‑39 (MQ=255) cAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTa > 1:574708/1‑45 (MQ=255) cAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAg < 1:1528557/69‑1 (MQ=255) cAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGc < 1:1600824/70‑1 (MQ=255) ccAGAGTTTTGAAGCCATTACCCTGGATAGCGgag < 1:2987294/35‑1 (MQ=255) ccAGAGTTTTGAAGCCATTACCCTGGATAGCGgag < 1:3270136/35‑1 (MQ=255) cAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCt > 1:5316754/1‑52 (MQ=255) aagagTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAg > 1:2641007/2‑71 (MQ=255) agagTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGact < 1:2794389/71‑3 (MQ=255) agagTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAg > 1:5312850/1‑70 (MQ=255) agagTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGt < 1:1759384/71‑1 (MQ=255) agTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTaa < 1:5371059/71‑1 (MQ=255) agTTTTAAATCCATTAGTCTGGATTGCAGAGAAGTGTACGAac < 1:2637403/43‑1 (MQ=16) agTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTaa < 1:4061192/71‑1 (MQ=16) gTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAAt < 1:2078635/71‑1 (MQ=255) gTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAAt < 1:5075619/71‑1 (MQ=255) gTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAAt < 1:5394219/71‑1 (MQ=255) gTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAAt > 1:2661813/1‑71 (MQ=255) gTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAAt < 1:1547175/71‑1 (MQ=16) ttttGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATg < 1:4816761/71‑1 (MQ=255) tttAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGccg < 1:5545042/55‑2 (MQ=16) ttGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGa < 1:2205268/70‑1 (MQ=255) tGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAg > 1:1975074/1‑71 (MQ=255) gAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATc > 1:661413/1‑42 (MQ=255) | AACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAG > Exported/1211160‑1211298 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |