Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,211,232 G→A 17.4% G40G (GGC→GGT cspC ← stress protein, member of the CspA‑family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,211,2320GA17.4% 288.8 / 45.6 127G40G (GGC→GGTcspCstress protein, member of the CspA‑family
Reads supporting (aligned to +/- strand):  ref base G (53/51);  new base A (4/18);  total (57/70)
Fisher's exact test for biased strand distribution p-value = 4.99e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 127 total aligned reads displayed.

AGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCC  >  Exported/1211163‑1211300
                                                                     |                                                                    
aGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGc                                                                     <  1:2106075/71‑1 (MQ=255)
aGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGc                                                                     <  1:5466415/71‑1 (MQ=255)
    gCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCAt                                                                  >  1:4532151/1‑70 (MQ=255)
     ccGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTa                                                                >  1:3423870/1‑71 (MQ=255)
     ccGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTa                                                                <  1:1369501/71‑1 (MQ=255)
      cGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGcc                                                                    <  1:3180194/66‑1 (MQ=255)
          ccTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTg                                                           <  1:773513/71‑1 (MQ=255)
           cTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTAc                                                               >  1:98333/1‑66 (MQ=255)
              tCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGa                                                         >  1:626907/1‑69 (MQ=255)
              tCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGa                                                         <  1:2718993/69‑1 (MQ=255)
                  gCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCATCCAGAGTTTTGAAGCCATTACCCTGGATAGc                                                     <  1:4264330/69‑1 (MQ=255)
                  gCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAg                                                      <  1:5100298/68‑1 (MQ=255)
                    cGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGgag                                                 <  1:3148808/71‑1 (MQ=255)
                    cGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGgag                                                 <  1:1933932/71‑1 (MQ=255)
                     gTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGa                                                         >  1:63875/1‑62 (MQ=255)
                     gTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGgag                                                 >  1:3579978/1‑70 (MQ=255)
                     gTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATAACCCTGGa                                                         >  1:321761/1‑62 (MQ=255)
                      tCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGc                                                                     <  1:181383/49‑1 (MQ=255)
                      tCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTAccc                                                             <  1:2603352/57‑1 (MQ=255)
                      tCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGgag                                                 >  1:854272/1‑69 (MQ=255)
                      tCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGga                                                  >  1:1729316/1‑68 (MQ=255)
                       ccTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAg                                              >  1:2486325/1‑71 (MQ=255)
                        cTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTg                                                           <  1:1263722/57‑1 (MQ=255)
                         tGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGcc                                                                    <  1:3249401/47‑1 (MQ=255)
                         tGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAgtg                                            >  1:57372/1‑71 (MQ=255)
                         gtgaTTTCGAACTCTACGCGCTGACCTTCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAgtg                                            <  1:3133154/68‑1 (MQ=255)
                           aaTTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTa                                                                >  1:4811216/1‑49 (MQ=255)
                           aaTTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAgtg                                            <  1:5304883/69‑1 (MQ=255)
                           aaTTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTa                                          <  1:5534542/71‑1 (MQ=255)
                           aaTTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTa                                          >  1:5393203/1‑71 (MQ=255)
                           aaTTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTa                                          <  1:1780813/71‑1 (MQ=255)
                            aTTTCGAACTCTACGCGCTGACCTTCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTAc                                         >  1:1514981/1‑71 (MQ=255)
                            aTTTCGAACTCTACGCGCTGACCTTCAGCAAGAGTTTCAAAACCATTAGTCTGGATTGCAGAGAAgtg                                            <  1:1848828/68‑1 (MQ=255)
                             tttCGAACTCTACGCGCTGACCTTCAGCAAGAGTTTTAAAACCAt                                                                  <  1:2096822/45‑1 (MQ=255)
                             tttCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTAc                                                               <  1:3847426/48‑1 (MQ=255)
                             tttCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTAc                                                               <  1:4815252/48‑1 (MQ=255)
                             tttCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACg                                        <  1:2343692/71‑1 (MQ=255)
                             tttCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACg                                        <  1:2215967/71‑1 (MQ=255)
                             tttCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACg                                        <  1:394843/71‑1 (MQ=255)
                             tttCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACg                                        <  1:1315390/71‑1 (MQ=255)
                               tCGAACTCTACGCGCTGACCTTCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGa                                       <  1:2607437/70‑1 (MQ=255)
                               tCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTAcc                                                              >  1:2481993/1‑47 (MQ=255)
                                  aaCTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAgtg                                            <  1:4910506/62‑1 (MQ=255)
                                  aaCTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAgtg                                            <  1:2621380/62‑1 (MQ=255)
                                   aCTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTAcc                                                              >  1:3794890/1‑43 (MQ=255)
                                   aCTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTAcc                                                              >  1:5322828/1‑43 (MQ=255)
                                    cTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGc                                                                     <  1:5246201/35‑1 (MQ=255)
                                       aaCGTTCTGACCTTCAGCCAGAGTTTTGAAGCCAt                                                                  >  1:530325/1‑35 (MQ=255)
                                       aaCGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTgg                                                          >  1:532067/1‑43 (MQ=255)
                                       aaCGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGa                                                         >  1:2265028/1‑44 (MQ=255)
                                       aaCGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCtt                              >  1:4738548/1‑71 (MQ=255)
                                         ctttCTGACCTTCGTCCAGAGATTTGTAACCATCGTTCTGGATAGCAGAGAAgt                                             >  1:3540719/3‑54 (MQ=255)
                                         cGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTAcc                                                              >  1:5516915/1‑37 (MQ=255)
                                         cGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTAcc                                                              >  1:148853/1‑37 (MQ=255)
                                         cGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTAcc                                                              >  1:2389265/1‑37 (MQ=255)
                                         cGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTtg                            <  1:1692410/71‑1 (MQ=255)
                                          tttCTGACCTTCGTCCAGAGATTTGTAACCATCGTTCTGGATAGCAgaga                                                <  1:3311892/49‑1 (MQ=255)
                                          gcgcTGACCTTCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCttt                             <  1:59939/66‑1 (MQ=255)
                                          gTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTAcc                                                              >  1:2607791/1‑36 (MQ=255)
                                           cgcTGACCTTCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCTTtgc                           <  1:5022279/68‑1 (MQ=255)
                                            tCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTtgctg                         >  1:4519688/1‑71 (MQ=255)
                                            tCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTtgctg                         >  1:5603969/1‑71 (MQ=255)
                                             cTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTtgctgc                        >  1:1254573/1‑71 (MQ=255)
                                              tGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTtgctgc                        >  1:468109/1‑70 (MQ=255)
                                               gACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCa                      >  1:5551196/1‑71 (MQ=255)
                                               gACCTTCAGCAAGAGTTTTAAAACCATTAGTCTGGa                                                         >  1:2001823/1‑36 (MQ=16)
                                                   ttCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTa                                          <  1:4446477/47‑1 (MQ=255)
                                                   ttCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTa                                          <  1:3684160/47‑1 (MQ=255)
                                                   ttCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTa                                          <  1:138043/47‑1 (MQ=255)
                                                   ttCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTa                                          <  1:1258506/47‑1 (MQ=255)
                                                   ttCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTa                                          <  1:5523298/47‑1 (MQ=255)
                                                    tCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCtt                              <  1:1788695/58‑1 (MQ=255)
                                                    tCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCtt                              <  1:5328213/58‑1 (MQ=255)
                                                    tCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCtt                              <  1:3821948/58‑1 (MQ=255)
                                                     cAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGgaga                                                >  1:1257439/1‑39 (MQ=255)
                                                     cAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTa                                          >  1:574708/1‑45 (MQ=255)
                                                     cAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAg                  <  1:1528557/69‑1 (MQ=255)
                                                     cAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAg                  <  1:3489174/69‑1 (MQ=255)
                                                     cAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGc                 <  1:1600824/70‑1 (MQ=255)
                                                        ccAGAGTTTTGAAGCCATTACCCTGGATAGCGgag                                                 <  1:3270136/35‑1 (MQ=255)
                                                        ccAGAGTTTTGAAGCCATTACCCTGGATAGCGgag                                                 <  1:2987294/35‑1 (MQ=255)
                                                         cAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCt                               >  1:5316754/1‑52 (MQ=255)
                                                         aagagTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAg            >  1:2641007/2‑71 (MQ=255)
                                                          agagTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGact           <  1:2794389/71‑3 (MQ=255)
                                                          agagTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGt           <  1:1759384/71‑1 (MQ=255)
                                                            agTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTaa         <  1:5371059/71‑1 (MQ=255)
                                                            agTTTTAAATCCATTAGTCTGGATTGCAGAGAAGTGTACGAac                                     <  1:2637403/43‑1 (MQ=16)
                                                            agTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTaa         <  1:4061192/71‑1 (MQ=16)
                                                             gTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAAt        <  1:5394219/71‑1 (MQ=255)
                                                             gTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAAt        <  1:2078635/71‑1 (MQ=255)
                                                             gTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAAt        >  1:2186337/1‑71 (MQ=255)
                                                             gTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAAt        <  1:5075619/71‑1 (MQ=255)
                                                             gTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAAt        >  1:2661813/1‑71 (MQ=255)
                                                             gTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAAt        <  1:1547175/71‑1 (MQ=16)
                                                                ttGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGa      <  1:2205268/70‑1 (MQ=255)
                                                                ttGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGa      <  1:2655222/70‑1 (MQ=255)
                                                                ttGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGa      <  1:1533115/70‑1 (MQ=255)
                                                                 tGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAg    >  1:1975074/1‑71 (MQ=255)
                                                                  gAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATc                                >  1:661413/1‑42 (MQ=255)
                                                                    aGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGcc  >  1:431655/1‑70 (MQ=255)
                                                                     |                                                                    
AGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCC  >  Exported/1211163‑1211300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: