Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,211,239 C→G 20.7% G38A (GGT→GCT) ‡ cspC ← stress protein, member of the CspA‑family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,211,2390CG20.7% 229.0 / 54.1 114G38A (GGT→GCT) ‡cspCstress protein, member of the CspA‑family
Reads supporting (aligned to +/- strand):  ref base C (39/50);  new base G (5/18);  total (45/69)
Fisher's exact test for biased strand distribution p-value = 5.95e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 131 total aligned reads displayed.

CCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTT  >  Exported/1211173‑1211308
                                                                  |                                                                     
 cTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTAc                                                                       >  1:98333/1‑66 (MQ=255)
   ttCTGGACGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGa                                                                 >  1:1498086/1‑70 (MQ=255)
    tCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGa                                                                 >  1:626907/1‑69 (MQ=255)
    tCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGa                                                                 >  1:2660368/1‑69 (MQ=255)
        gCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCATCCAGAGTTTTGAAGCCATTACCCTGGATAGc                                                             <  1:4264330/69‑1 (MQ=255)
        gCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCg                                                            <  1:1299178/70‑1 (MQ=255)
          cGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGgag                                                         <  1:1933932/71‑1 (MQ=255)
           gTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGgag                                                         >  1:3579978/1‑70 (MQ=255)
           gTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATAACCCTGGa                                                                 >  1:321761/1‑62 (MQ=255)
            tCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTAccc                                                                     <  1:2603352/57‑1 (MQ=255)
            tCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGgag                                                         >  1:854272/1‑69 (MQ=255)
            tCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGga                                                          >  1:1729316/1‑68 (MQ=255)
              cTGAATGTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAgt                                                     >  1:739256/1‑71 (MQ=255)
               tGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAgtg                                                    >  1:57372/1‑71 (MQ=255)
               gtgaTTTCGAACTCTACGCGCTGACCTTCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAgtg                                                    <  1:3133154/68‑1 (MQ=255)
                 aaTTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAgtg                                                    <  1:5304883/69‑1 (MQ=255)
                 aaTTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTa                                                  <  1:5534542/71‑1 (MQ=255)
                 aaTTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTa                                                  >  1:5393203/1‑71 (MQ=255)
                 aaTTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTa                                                  <  1:1780813/71‑1 (MQ=255)
                  aTTTCGAACTCTACGCGCTGACCTTCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTAc                                                 >  1:1514981/1‑71 (MQ=255)
                  aTTTCGAACTCTACGCGCTGACCTTCAGCAAGAGTTTCAAAACCATTAGTCTGGATTGCAGAGAAgtg                                                    <  1:1848828/68‑1 (MQ=255)
                  aTTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTa                                                  <  1:5208823/70‑1 (MQ=255)
                  aTTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTAc                                                 >  1:3218852/1‑71 (MQ=255)
                   tttCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTAc                                                                       <  1:3847426/48‑1 (MQ=255)
                   tttCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTAc                                                                       <  1:4815252/48‑1 (MQ=255)
                   tttCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACg                                                <  1:2343692/71‑1 (MQ=255)
                   tttCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACg                                                <  1:367860/71‑1 (MQ=255)
                   tttCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACg                                                <  1:1315390/71‑1 (MQ=255)
                   tttCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACg                                                <  1:394843/71‑1 (MQ=255)
                   tttCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACg                                                <  1:2215967/71‑1 (MQ=255)
                     tCGAACTCTACGCGCTGACCTTCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGa                                               <  1:2607437/70‑1 (MQ=255)
                     tCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTAcc                                                                      >  1:2481993/1‑47 (MQ=255)
                        aaCTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAgtg                                                    <  1:2621380/62‑1 (MQ=255)
                        aaCTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAgtg                                                    <  1:4910506/62‑1 (MQ=255)
                        aaCTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAaca                                            >  1:202511/1‑70 (MQ=255)
                        aaCTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAaca                                            >  1:2992507/1‑70 (MQ=255)
                         aCTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTAcc                                                                      >  1:3794890/1‑43 (MQ=255)
                         aCTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTAcc                                                                      >  1:5322828/1‑43 (MQ=255)
                             aaCGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTgg                                                                  >  1:532067/1‑43 (MQ=255)
                             aaCGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGa                                                                 >  1:2265028/1‑44 (MQ=255)
                             aaCGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCtt                                      >  1:4738548/1‑71 (MQ=255)
                              aCGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGgag                                                         <  1:4306100/51‑1 (MQ=255)
                               cGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTAcc                                                                      >  1:2389265/1‑37 (MQ=255)
                               cGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTAcc                                                                      >  1:5516915/1‑37 (MQ=255)
                               cGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTAcc                                                                      >  1:1834766/1‑37 (MQ=255)
                               cGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTg                                                                   <  1:3418119/40‑1 (MQ=255)
                                gcgcTGACCTTCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCttt                                     <  1:59939/66‑1 (MQ=255)
                                 ttCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTtgct                                  >  1:1588597/1‑71 (MQ=255)
                                 cgcTGACCTTCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCTTtgc                                   <  1:5022279/68‑1 (MQ=255)
                                  tCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTtgctg                                 >  1:4519688/1‑71 (MQ=255)
                                  tCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTtgctg                                 >  1:5603969/1‑71 (MQ=255)
                                   cTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTtgctgc                                >  1:1254573/1‑71 (MQ=255)
                                    tGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTtgctgc                                >  1:468109/1‑70 (MQ=255)
                                     gACCTTCAGCAAGAGTTTTAAAACCATTAGTCTGGa                                                                 >  1:2001823/1‑36 (MQ=16)
                                         ttCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTa                                                  <  1:1258506/47‑1 (MQ=255)
                                         ttCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTa                                                  <  1:138043/47‑1 (MQ=255)
                                         ttCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTa                                                  <  1:4446477/47‑1 (MQ=255)
                                         ttCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTa                                                  <  1:5523298/47‑1 (MQ=255)
                                          tCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGc                         >  1:3933927/1‑71 (MQ=255)
                                          tCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGc                         >  1:2276753/1‑71 (MQ=255)
                                          tCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCtt                                      <  1:3821948/58‑1 (MQ=255)
                                          tCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCtt                                      <  1:5328213/58‑1 (MQ=255)
                                          tCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCtt                                      <  1:1788695/58‑1 (MQ=255)
                                           cAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGgaga                                                        >  1:1257439/1‑39 (MQ=255)
                                           cAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTa                                                  >  1:574708/1‑45 (MQ=255)
                                           cAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAg                          <  1:1528557/69‑1 (MQ=255)
                                           cAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAg                          <  1:3489174/69‑1 (MQ=255)
                                           cAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGc                         <  1:1600824/70‑1 (MQ=255)
                                            aGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCg                       <  1:2632015/71‑1 (MQ=255)
                                              ccAGAGTTTTGAAGCCATTACCCTGGATAGCGgag                                                         <  1:3270136/35‑1 (MQ=255)
                                              ccAGAGTTTTGAAGCCATTACCCTGGATAGCGgag                                                         <  1:2987294/35‑1 (MQ=255)
                                                agagTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGact                   <  1:2794389/71‑3 (MQ=255)
                                                agagTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAg                    >  1:5312850/1‑70 (MQ=255)
                                                agagTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGt                   <  1:1759384/71‑1 (MQ=255)
                                                  agTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTaa                 <  1:5371059/71‑1 (MQ=255)
                                                  agTTTTAAATCCATTAGTCTGGATTGCAGAGAAGTGTACGAac                                             <  1:2637403/43‑1 (MQ=16)
                                                   gTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAAt                <  1:5075619/71‑1 (MQ=255)
                                                   gTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAAt                >  1:2661813/1‑71 (MQ=255)
                                                   gTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAAt                >  1:2186337/1‑71 (MQ=255)
                                                   gTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAAt                <  1:5394219/71‑1 (MQ=255)
                                                   gTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAAt                <  1:1547175/71‑1 (MQ=16)
                                                     tttAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGccg                              <  1:5545042/55‑2 (MQ=16)
                                                      ttGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGa              <  1:2205268/70‑1 (MQ=255)
                                                      ttGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGa              <  1:2655222/70‑1 (MQ=255)
                                                        gAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATc                                        >  1:661413/1‑42 (MQ=255)
                                                         aaaccATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAAcc          <  1:663283/68‑1 (MQ=12)
                                                            ccATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCttt                                     >  1:1058512/1‑41 (MQ=19)
                                                            ccATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCaaa       <  1:2066571/71‑1 (MQ=255)
                                                             cATTGCTCTGGATGGCGGTGAAATGGACGAAAACGTCTTTGCTGCCATCATCAGGa                     >  1:2481815/1‑56 (MQ=2)
                                                             cATTGCTCTGGATGGCGGTGAAATGGACGAAAACGTCTTTGCTGCCATCATCAGGa                     >  1:3652710/1‑56 (MQ=2)
                                                             cATTGCTCTGGATGGCGGTGAAATGGACGAAAACGTCTTTGCTGCCATCATCAGGAGTGATAAAGCCaaa       <  1:951577/70‑1 (MQ=9)
                                                             cATTGCTCTGGATGGCGGTGAAATGGACGAAAACGTCTTTGCTGCCATCATCAGGAGTGATAAAGCCaaa       <  1:5092732/70‑1 (MQ=9)
                                                             cATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGa                     >  1:2581149/1‑56 (MQ=12)
                                                              aTTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAAc     >  1:3805245/1‑71 (MQ=255)
                                                              aTTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAAc     >  1:597120/1‑71 (MQ=255)
                                                               ttACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCaa        <  1:3383326/67‑1 (MQ=255)
                                                                 aCCCTGGATAGCGGAGAAGTGTACGAACACATCtt                                      <  1:4364757/35‑1 (MQ=255)
                                                                 aCCCTGGATAGCGGAGAAGTGTACGAACACATCtt                                      <  1:2290882/35‑1 (MQ=255)
                                                                  cccTGGATAGCGGAGAAGTGTACGAACACATCTTTGATGCCATCAGCCGGAGTAATGAAGCCAAAACCtt  >  1:4280542/1‑70 (MQ=255)
                                                                  cccTGGATAGCGGAGAAGTGTAAGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCtt  <  1:4050024/70‑1 (MQ=255)
                                                                  |                                                                     
CCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTT  >  Exported/1211173‑1211308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: