Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,211,247 A→T 26.1% A35A (GCT→GCA cspC ← stress protein, member of the CspA‑family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,211,2470AT26.1% 217.3 / 78.7 117A35A (GCT→GCAcspCstress protein, member of the CspA‑family
Reads supporting (aligned to +/- strand):  ref base A (36/46);  new base T (8/22);  total (46/71)
Fisher's exact test for biased strand distribution p-value = 1.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 117 total aligned reads displayed.

GCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACT  >  Exported/1211181‑1211314
                                                                  |                                                                   
gCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCATCCAGAGTTTTGAAGCCATTACCCTGGATAGc                                                                   <  1:4264330/69‑1 (MQ=255)
gCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAg                                                                    <  1:5100298/68‑1 (MQ=255)
gCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCg                                                                  <  1:1299178/70‑1 (MQ=255)
  cGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGgag                                                               <  1:3148808/71‑1 (MQ=255)
  cGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGgag                                                               <  1:1933932/71‑1 (MQ=255)
    tCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGgag                                                               >  1:854272/1‑69 (MQ=255)
    tCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGga                                                                >  1:1729316/1‑68 (MQ=255)
     ccTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAg                                                            >  1:2486325/1‑71 (MQ=255)
      cTGAATGTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAgt                                                           >  1:739256/1‑71 (MQ=255)
       tGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAgtg                                                          >  1:57372/1‑71 (MQ=255)
       gtgaTTTCGAACTCTACGCGCTGACCTTCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAgtg                                                          <  1:3133154/68‑1 (MQ=255)
         aaTTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAgtg                                                          <  1:5304883/69‑1 (MQ=255)
         aaTTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTa                                                        <  1:1780813/71‑1 (MQ=255)
         aaTTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTa                                                        >  1:5393203/1‑71 (MQ=255)
          aTTTCGAACTCTACGCGCTGACCTTCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTAc                                                       >  1:1514981/1‑71 (MQ=255)
          aTTTCGAACTCTACGCGCTGACCTTCAGCAAGAGTTTCAAAACCATTAGTCTGGATTGCAGAGAAgtg                                                          <  1:1848828/68‑1 (MQ=255)
          aTTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTa                                                        <  1:5208823/70‑1 (MQ=255)
          aTTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTAc                                                       >  1:3218852/1‑71 (MQ=255)
           tttCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACg                                                      <  1:394843/71‑1 (MQ=255)
           tttCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACg                                                      <  1:367860/71‑1 (MQ=255)
           tttCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACg                                                      <  1:2215967/71‑1 (MQ=255)
             tCGAACTCTACGCGCTGACCTTCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGa                                                     <  1:2607437/70‑1 (MQ=255)
                aaCTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAgtg                                                          <  1:2621380/62‑1 (MQ=255)
                aaCTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAgtg                                                          <  1:4910506/62‑1 (MQ=255)
                aaCTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAaca                                                  >  1:202511/1‑70 (MQ=255)
                aaCTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAaca                                                  >  1:2992507/1‑70 (MQ=255)
                     aaCGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCtt                                            >  1:4738548/1‑71 (MQ=255)
                      aCGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGgag                                                               <  1:4306100/51‑1 (MQ=255)
                       ctttCTGACCTTCGTCCAGAGATTTGTAACCATCGTTCTGGATAGCAGAGAAgt                                                           >  1:3540719/3‑54 (MQ=255)
                       cGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTtg                                          <  1:1692410/71‑1 (MQ=255)
                        tttCTGACCTTCGTCCAGAGATTTGTAACCATCGTTCTGGATAGCAgaga                                                              <  1:3311892/49‑1 (MQ=255)
                         cgcTGACCTTCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCTTtgc                                         <  1:5022279/68‑1 (MQ=255)
                          tCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTtgctg                                       >  1:4519688/1‑71 (MQ=255)
                          tCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTtgctg                                       >  1:5603969/1‑71 (MQ=255)
                           cTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTtgctgc                                      >  1:1254573/1‑71 (MQ=255)
                             gACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCa                                    >  1:5551196/1‑71 (MQ=255)
                                 ttCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTa                                                        <  1:4446477/47‑1 (MQ=255)
                                 ttCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTa                                                        <  1:1258506/47‑1 (MQ=255)
                                 ttCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTa                                                        <  1:5523298/47‑1 (MQ=255)
                                 ttCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTa                                                        <  1:3684160/47‑1 (MQ=255)
                                 ttCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTa                                                        <  1:138043/47‑1 (MQ=255)
                                  tCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGc                               >  1:2276753/1‑71 (MQ=255)
                                  tCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGc                               >  1:3933927/1‑71 (MQ=255)
                                  tCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCtt                                            <  1:3821948/58‑1 (MQ=255)
                                  tCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCtt                                            <  1:1788695/58‑1 (MQ=255)
                                  tCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCtt                                            <  1:5328213/58‑1 (MQ=255)
                                   cAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGgaga                                                              >  1:1257439/1‑39 (MQ=255)
                                   cAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTa                                                        >  1:574708/1‑45 (MQ=255)
                                   cAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAg                                <  1:3489174/69‑1 (MQ=255)
                                   cAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAg                                <  1:1528557/69‑1 (MQ=255)
                                   cAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGc                               <  1:1600824/70‑1 (MQ=255)
                                      ccAGAGTTTTGAAGCCATTACCCTGGATAGCGgag                                                               <  1:2987294/35‑1 (MQ=255)
                                       aagagTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAg                          >  1:2641007/2‑71 (MQ=255)
                                        agagTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGact                         <  1:2794389/71‑3 (MQ=255)
                                        agagTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAg                          >  1:5312850/1‑70 (MQ=255)
                                        agagTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGt                         <  1:1759384/71‑1 (MQ=255)
                                          agTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTaa                       <  1:5371059/71‑1 (MQ=255)
                                          agTTTTAAATCCATTAGTCTGGATTGCAGAGAAGTGTACGAac                                                   <  1:2637403/43‑1 (MQ=16)
                                          agTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTaa                       <  1:4061192/71‑1 (MQ=16)
                                           gTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAAt                      >  1:2186337/1‑71 (MQ=255)
                                           gTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAAt                      <  1:2078635/71‑1 (MQ=255)
                                           gTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAAt                      <  1:5075619/71‑1 (MQ=255)
                                           gTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAAt                      <  1:5394219/71‑1 (MQ=255)
                                           gTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAAt                      >  1:2661813/1‑71 (MQ=255)
                                           gTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAAt                      <  1:1547175/71‑1 (MQ=16)
                                            ttttGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATg                     <  1:4816761/71‑1 (MQ=255)
                                             tttAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGccg                                    <  1:5545042/55‑2 (MQ=16)
                                              ttGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGa                    <  1:2655222/70‑1 (MQ=255)
                                              ttGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGa                    <  1:2205268/70‑1 (MQ=255)
                                              ttGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGa                    <  1:1533115/70‑1 (MQ=255)
                                               tGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAg                  >  1:1975074/1‑71 (MQ=255)
                                                gAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATc                                              >  1:661413/1‑42 (MQ=255)
                                                 aaaccATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAAcc                <  1:663283/68‑1 (MQ=12)
                                                  aGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGcc                >  1:431655/1‑70 (MQ=255)
                                                    ccATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCttt                                           >  1:1058512/1‑41 (MQ=19)
                                                    ccATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCaaa             <  1:2066571/71‑1 (MQ=255)
                                                    ccATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCaaa             <  1:2245700/71‑1 (MQ=255)
                                                     cATTGCTCTGGATGGCGGTGAAATGGACGAAAACGTCTTTGCTGCCATCATCAGGa                           >  1:2481815/1‑56 (MQ=2)
                                                     cATTGCTCTGGATGGCGGTGAAATGGACGAAAACGTCTTTGCTGCCATCATCAGGa                           >  1:3652710/1‑56 (MQ=2)
                                                     cATTGCTCTGGATGGCGGTGAAATGGACGAAAACGTCTTTGCTGCCATCATCAGGAGTGATAAAGCCaaa             <  1:951577/70‑1 (MQ=9)
                                                     cATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGa                           >  1:2581149/1‑56 (MQ=12)
                                                      aTTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAAc           >  1:597120/1‑71 (MQ=255)
                                                      aTTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAAc           >  1:3805245/1‑71 (MQ=255)
                                                       ttACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCaa              <  1:3383326/67‑1 (MQ=255)
                                                         aCCCTGGATAGCGGAGAAGTGTACGAACACATCtt                                            <  1:2290882/35‑1 (MQ=255)
                                                         aCCCTGGATAGCGGAGAAGTGTACGAACACATCtt                                            <  1:2382160/35‑1 (MQ=255)
                                                         aCCCTGGATAGCGGAGAAGTGTACGAACACATCtt                                            <  1:4364757/35‑1 (MQ=255)
                                                          gtcTGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCtt        <  1:4722556/68‑1 (MQ=12)
                                                          gtcTGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCtt        <  1:1279319/68‑1 (MQ=12)
                                                          cccTGGATAGCGGAGAAGTGTAAGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCtt        <  1:4050024/70‑1 (MQ=255)
                                                           tcTGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATcc          <  1:35234/66‑1 (MQ=12)
                                                            cTGGATAGCGGAGAAGTGTACGAACACATCTTTTCTGCCATCAGCCGGAGTAATGAAGCCAAAACCtt        >  1:2917133/1‑68 (MQ=255)
                                                            cTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAg     <  1:3836516/71‑1 (MQ=255)
                                                             tGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGaaac                 >  1:5419329/1‑56 (MQ=18)
                                                             tGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCtt        >  1:4297971/1‑67 (MQ=12)
                                                              ggATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGAc   >  1:1426323/1‑71 (MQ=255)
                                                               gATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGa    >  1:4172713/1‑69 (MQ=255)
                                                               gATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACt  <  1:523556/71‑1 (MQ=12)
                                                               gATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACt  >  1:2843540/1‑71 (MQ=255)
                                                                aTAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCgg                            >  1:5179639/1‑44 (MQ=255)
                                                                  |                                                                   
GCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACT  >  Exported/1211181‑1211314

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: