Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | Exported | 1,211,250 | G→A | 26.9% | S34S (TCC→TCT) | cspC ← | stress protein, member of the CspA‑family |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | Exported | 1,211,250 | 0 | G | A | 26.9% | 194.7 / 75.4 | 119 | S34S (TCC→TCT) | cspC | stress protein, member of the CspA‑family |
Reads supporting (aligned to +/- strand): ref base G (38/49); new base A (9/23); total (47/72) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.43e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 123 total aligned reads displayed.
GCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGA > Exported/1211181‑1211320 | cGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGgag < 1:3148808/71‑1 (MQ=255) cGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGgag < 1:1933932/71‑1 (MQ=255) gTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGgag > 1:3579978/1‑70 (MQ=255) tCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGgag > 1:854272/1‑69 (MQ=255) tCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGga > 1:1729316/1‑68 (MQ=255) ccTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAg > 1:2486325/1‑71 (MQ=255) cTGAATGTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAgt > 1:739256/1‑71 (MQ=255) tGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAgtg > 1:57372/1‑71 (MQ=255) gtgaTTTCGAACTCTACGCGCTGACCTTCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAgtg < 1:3133154/68‑1 (MQ=255) aaTTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAgtg < 1:5304883/69‑1 (MQ=255) aaTTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTa < 1:5534542/71‑1 (MQ=255) aaTTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTa > 1:5393203/1‑71 (MQ=255) aTTTCGAACTCTACGCGCTGACCTTCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTAc > 1:1514981/1‑71 (MQ=255) aTTTCGAACTCTACGCGCTGACCTTCAGCAAGAGTTTCAAAACCATTAGTCTGGATTGCAGAGAAgtg < 1:1848828/68‑1 (MQ=255) aTTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTa < 1:5208823/70‑1 (MQ=255) tttCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACg < 1:394843/71‑1 (MQ=255) tttCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACg < 1:2215967/71‑1 (MQ=255) tttCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACg < 1:1315390/71‑1 (MQ=255) tttCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACg < 1:367860/71‑1 (MQ=255) aaCTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAgtg < 1:4910506/62‑1 (MQ=255) aaCTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAgtg < 1:2621380/62‑1 (MQ=255) aaCTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAaca > 1:202511/1‑70 (MQ=255) aaCTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAaca > 1:2992507/1‑70 (MQ=255) aaCGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCtt > 1:4738548/1‑71 (MQ=255) aCGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGgag < 1:4306100/51‑1 (MQ=255) cGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTtg < 1:1692410/71‑1 (MQ=255) tttCTGACCTTCGTCCAGAGATTTGTAACCATCGTTCTGGATAGCAgaga < 1:3311892/49‑1 (MQ=255) gcgcTGACCTTCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCttt < 1:59939/66‑1 (MQ=255) ttCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTtgct > 1:1588597/1‑71 (MQ=255) cgcTGACCTTCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCTTtgc < 1:5022279/68‑1 (MQ=255) tCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTtgctg > 1:4519688/1‑71 (MQ=255) cTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTtgctgc > 1:1254573/1‑71 (MQ=255) tGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTtgctgc > 1:468109/1‑70 (MQ=255) gACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCa > 1:5551196/1‑71 (MQ=255) ttCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTa < 1:1258506/47‑1 (MQ=255) ttCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTa < 1:138043/47‑1 (MQ=255) ttCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTa < 1:5523298/47‑1 (MQ=255) tCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGc > 1:3933927/1‑71 (MQ=255) tCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGc > 1:2276753/1‑71 (MQ=255) tCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCtt < 1:3821948/58‑1 (MQ=255) tCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCtt < 1:1788695/58‑1 (MQ=255) tCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCtt < 1:5328213/58‑1 (MQ=255) cAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGgaga > 1:1257439/1‑39 (MQ=255) cAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTa > 1:574708/1‑45 (MQ=255) cAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAg < 1:3489174/69‑1 (MQ=255) cAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGc < 1:1600824/70‑1 (MQ=255) aGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCg < 1:2632015/71‑1 (MQ=255) ccAGAGTTTTGAAGCCATTACCCTGGATAGCGgag < 1:3270136/35‑1 (MQ=255) aagagTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAg > 1:2641007/2‑71 (MQ=255) agagTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGact < 1:2794389/71‑3 (MQ=255) agagTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAg > 1:5312850/1‑70 (MQ=255) agagTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGt < 1:1759384/71‑1 (MQ=255) agTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTaa < 1:5371059/71‑1 (MQ=255) agTTTTAAATCCATTAGTCTGGATTGCAGAGAAGTGTACGAac < 1:2637403/43‑1 (MQ=16) agTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTaa < 1:4061192/71‑1 (MQ=16) gTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAAt < 1:2078635/71‑1 (MQ=255) gTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAAt < 1:5075619/71‑1 (MQ=255) gTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAAt > 1:2186337/1‑71 (MQ=255) gTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAAt < 1:5394219/71‑1 (MQ=255) gTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAAt < 1:1547175/71‑1 (MQ=16) tttAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGccg < 1:5545042/55‑2 (MQ=16) ttGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGa < 1:1533115/70‑1 (MQ=255) ttGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGa < 1:2655222/70‑1 (MQ=255) aaaccATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAAcc < 1:663283/68‑1 (MQ=12) aGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGcc > 1:431655/1‑70 (MQ=255) ccATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCttt > 1:1058512/1‑41 (MQ=19) ccATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCaaa < 1:2066571/71‑1 (MQ=255) ccATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCaaa < 1:2245700/71‑1 (MQ=255) cATTGCTCTGGATGGCGGTGAAATGGACGAAAACGTCTTTGCTGCCATCATCAGGa > 1:3652710/1‑56 (MQ=2) cATTGCTCTGGATGGCGGTGAAATGGACGAAAACGTCTTTGCTGCCATCATCAGGa > 1:2481815/1‑56 (MQ=2) cATTGCTCTGGATGGCGGTGAAATGGACGAAAACGTCTTTGCTGCCATCATCAGGAGTGATAAAGCCaaa < 1:5092732/70‑1 (MQ=9) cATTGCTCTGGATGGCGGTGAAATGGACGAAAACGTCTTTGCTGCCATCATCAGGAGTGATAAAGCCaaa < 1:951577/70‑1 (MQ=9) cATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGa > 1:2581149/1‑56 (MQ=12) aTTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAAc > 1:597120/1‑71 (MQ=255) aTTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAAc > 1:3805245/1‑71 (MQ=255) ttACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCaa < 1:3383326/67‑1 (MQ=255) aCCCTGGATAGCGGAGAAGTGTACGAACACATCtt < 1:4364757/35‑1 (MQ=255) aCCCTGGATAGCGGAGAAGTGTACGAACACATCtt < 1:1814370/35‑1 (MQ=255) aCCCTGGATAGCGGAGAAGTGTACGAACACATCtt < 1:2290882/35‑1 (MQ=255) aCCCTGGATAGCGGAGAAGTGTACGAACACATCtt < 1:2382160/35‑1 (MQ=255) gtcTGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCtt < 1:1279319/68‑1 (MQ=12) gtcTGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCtt < 1:4722556/68‑1 (MQ=12) ctcTGGATGGCGGTGAAATGGACGAAAACGTCTTTGCTGCCATCATCAGGAGTGATAAAGCCAAAACCttt < 1:2084618/69‑1 (MQ=9) cccTGGATAGCGGAGAAGTGTAAGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCtt < 1:4050024/70‑1 (MQ=255) tcTGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATcc < 1:35234/66‑1 (MQ=12) cTGGATAGCGGAGAAGTGTACGAACACATCTTTTCTGCCATCAGCCGGAGTAATGAAGCCAAAACCtt > 1:2917133/1‑68 (MQ=255) cTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAg < 1:3836516/71‑1 (MQ=255) tGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCtt > 1:4297971/1‑67 (MQ=12) tGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGa > 1:290164/1‑71 (MQ=255) ggATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGAc > 1:1426323/1‑71 (MQ=255) gATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGa > 1:4172713/1‑69 (MQ=255) gATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACt > 1:2843540/1‑71 (MQ=255) gATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACt < 1:523556/71‑1 (MQ=12) gCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGc > 1:3857525/1‑52 (MQ=255) gCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTa < 1:3655965/63‑1 (MQ=255) gCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGtt < 1:153090/71‑1 (MQ=255) cGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTa < 1:1732270/44‑1 (MQ=255) cGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTa < 1:5060615/62‑1 (MQ=255) ggAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGa < 1:5539536/71‑1 (MQ=255) ggAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGa < 1:303111/71‑1 (MQ=255) | GCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGA > Exported/1211181‑1211320 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |