Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | Exported | 1,211,304 | A→T | 32.2% | G16G (GGT→GGA) | cspC ← | stress protein, member of the CspA‑family |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | Exported | 1,211,304 | 0 | A | T | 32.2% | 201.1 / 122.7 | 134 | G16G (GGT→GGA) | cspC | stress protein, member of the CspA‑family |
Reads supporting (aligned to +/- strand): ref base A (44/46); new base T (22/21); total (67/67) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 8.54e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.62e-01 |
Only 100 of 144 total aligned reads displayed.
ATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTT > Exported/1211235‑1211373 | aTTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAAc > 1:597120/1‑71 (MQ=255) aTTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAAc > 1:3805245/1‑71 (MQ=255) gtcTGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCtt < 1:4722556/68‑1 (MQ=12) gtcTGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCtt < 1:1279319/68‑1 (MQ=12) ctcTGGATGGCGGTGAAATGGACGAAAACGTCTTTGCTGCCATCATCAGGAGTGATAAAGCCAAAACCttt < 1:2084618/69‑1 (MQ=9) cccTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCtt < 1:3886370/70‑1 (MQ=255) tcTGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATcc < 1:35234/66‑1 (MQ=12) cTGGATAGCGGAGAAGTGTACGAACACATCTTTTCTGCCATCAGCCGGAGTAATGAAGCCAAAACCtt > 1:2917133/1‑68 (MQ=255) tGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCtt > 1:4297971/1‑67 (MQ=12) tGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGa > 1:290164/1‑71 (MQ=255) gATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGa > 1:4172713/1‑69 (MQ=255) gATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACt < 1:523556/71‑1 (MQ=12) gCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTa < 1:3655965/63‑1 (MQ=255) gCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGtt < 1:153090/71‑1 (MQ=255) cagagaAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCAtta < 1:1167976/69‑2 (MQ=255) cagagaAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCAtt > 1:4202839/3‑70 (MQ=12) cGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTa < 1:5060615/62‑1 (MQ=255) ggAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGa < 1:5539536/71‑1 (MQ=255) ggAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGa < 1:1613290/71‑1 (MQ=255) ggAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGa < 1:303111/71‑1 (MQ=255) gagaAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGAc > 1:3861295/1‑63 (MQ=12) gagaAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGAc > 1:2598203/1‑63 (MQ=12) gagaAGTGTACGAACACGTCTTTGCTGCCGTCTTCAGGAGTAATGAAACCGAATCCtttg < 1:1869002/60‑2 (MQ=12) aaGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCtt < 1:3736081/55‑1 (MQ=255) tgtAAGAACACGTCATTGCTGCCGTCTTCCGGAGTAATGATACCGAATCCtt < 1:3246410/52‑1 (MQ=255) tACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTaa > 1:2964801/1‑71 (MQ=255) tACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTa > 1:3796598/1‑52 (MQ=255) tACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGt > 1:2812273/1‑59 (MQ=255) tACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTa < 1:2181620/70‑1 (MQ=255) tACGAACACATCTTTAGAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCGTTGAACCAtttta > 1:4551257/1‑69 (MQ=255) gaCGAAAACGTCTTTGCTGCCATCATCAGGAGTGATAAAGCCAAAAc > 1:893405/2‑47 (MQ=12) aCGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAAc > 1:2164978/1‑46 (MQ=255) cGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAAc > 1:2731558/1‑62 (MQ=9) cGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAAcg > 1:4127071/1‑70 (MQ=12) cGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAAc < 1:3081630/70‑1 (MQ=12) cGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAAcc < 1:4801092/71‑1 (MQ=255) gAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCt > 1:4944556/1‑71 (MQ=255) gAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCt > 1:3011678/1‑71 (MQ=255) acacATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGa < 1:3971008/71‑1 (MQ=255) acacATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGa < 1:3624785/71‑1 (MQ=255) cacaTCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCAc < 1:3855277/61‑1 (MQ=255) cacaTCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCAc < 1:476376/61‑1 (MQ=255) caTCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCt < 1:4781149/71‑1 (MQ=255) caTCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCt < 1:1152790/71‑1 (MQ=255) aTCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCtt < 1:4970200/71‑1 (MQ=255) tCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAg > 1:4588488/1‑43 (MQ=255) tCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTg < 1:5642545/51‑1 (MQ=255) tCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTa < 1:5158342/60‑1 (MQ=255) tCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTa < 1:4881688/60‑1 (MQ=255) cTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAAc > 1:4205672/1‑36 (MQ=255) cTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTaa > 1:5689306/1‑60 (MQ=255) tttGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCtttgg < 1:1025835/41‑3 (MQ=12) tttGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCtttg < 1:4885998/40‑2 (MQ=12) tttGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTaa > 1:4453267/1‑71 (MQ=255) tgctgcCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAACGTTACCTTTaa < 1:3695205/69‑1 (MQ=255) tgctgcCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTa < 1:3454643/68‑1 (MQ=255) tgctgcCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTa < 1:3678572/68‑1 (MQ=255) gctgcCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCt > 1:5137044/1‑71 (MQ=255) ctgcCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAACGTTACCTTTAATCtt < 1:5041135/71‑1 (MQ=255) ctgcCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAACGTTACCTTTAATCt < 1:3743808/70‑1 (MQ=255) tgcCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTa < 1:402581/65‑1 (MQ=255) ccATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGc > 1:1797797/1‑71 (MQ=255) aTCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCa < 1:5384946/71‑1 (MQ=255) tCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCt < 1:3575/64‑1 (MQ=255) tCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCt < 1:2535722/64‑1 (MQ=255) tCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCAt < 1:1346808/71‑1 (MQ=255) tCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCAt > 1:5457350/1‑71 (MQ=255) ttccGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCa > 1:4018360/3‑42 (MQ=255) ttccGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCa > 1:3968665/3‑42 (MQ=255) ccGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTa < 1:3480573/44‑1 (MQ=255) ccGGAGTAATGAAACCGAATCCTTTGGACTCATTaaa > 1:3854253/1‑37 (MQ=12) ccGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAAc > 1:2053871/1‑46 (MQ=16) ccGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAACGTTACCTTTAATCTTAGACAtcaa < 1:3476175/70‑4 (MQ=255) ccGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAACGTTACCTTTAATCTTAGACAtca < 1:3136418/69‑3 (MQ=255) ccGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAACGTTACCTTTAATCTTAGACAtca < 1:3627065/69‑3 (MQ=255) ggAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGaaa < 1:3067404/71‑1 (MQ=255) aGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAACGTTACCTTTAATCtta > 1:810974/1‑57 (MQ=16) gTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAACGTTAcc > 1:3366984/1‑47 (MQ=9) tAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCtt < 1:4339395/48‑1 (MQ=255) tAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCtt < 1:1822953/48‑1 (MQ=255) tAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGaaa < 1:4087591/67‑1 (MQ=255) aTGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTaa > 1:172183/1‑49 (MQ=255) aTGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTaa > 1:4980506/1‑49 (MQ=255) aTGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTcc < 1:3240707/71‑1 (MQ=255) aTGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTcc > 1:3116939/1‑71 (MQ=255) aTGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTcc > 1:3877377/1‑71 (MQ=255) aTGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTcc > 1:3092134/1‑71 (MQ=255) tGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCAt < 1:5568382/58‑1 (MQ=255) aaGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGaaaaa > 1:1909686/1‑64 (MQ=255) aaGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGaaaaa > 1:2473115/1‑64 (MQ=255) aaGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGaaaaa > 1:2466670/1‑64 (MQ=255) aaGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGaaaaa > 1:4413746/1‑64 (MQ=255) gCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCt > 1:2943908/1‑67 (MQ=255) ccAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTTcc < 1:2447139/51‑1 (MQ=255) aaaaCCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATc < 1:2119062/43‑1 (MQ=255) aaaaCCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCAt > 1:2056603/1‑51 (MQ=255) aaaaCCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTg < 1:3300760/71‑1 (MQ=255) aaaCCTTTAGACTCGTTGAACCACTTAACCTGACCTTTa < 1:56089/39‑1 (MQ=255) aaaCCTTTAGACTCGTTGAACCACTTAACCTGACCTTTa < 1:4757809/39‑1 (MQ=255) aaCCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGtt > 1:292420/1‑71 (MQ=255) | ATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTT > Exported/1211235‑1211373 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |