Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,211,316 G→A 26.3% N12N (AAC→AAT cspC ← stress protein, member of the CspA‑family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,211,3160GA26.3% 211.6 / 69.6 114N12N (AAC→AATcspCstress protein, member of the CspA‑family
Reads supporting (aligned to +/- strand):  ref base G (34/50);  new base A (17/13);  total (51/63)
Fisher's exact test for biased strand distribution p-value = 1.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 115 total aligned reads displayed.

GCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCG  >  Exported/1211248‑1211384
                                                                    |                                                                    
gCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGtt                                                                    <  1:153090/71‑1 (MQ=255)
 cagagaAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCAtta                                                                   <  1:1167976/69‑2 (MQ=255)
 cagagaAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCAtt                                                                    <  1:3159219/68‑1 (MQ=12)
 cagagaAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCAtt                                                                    >  1:4202839/3‑70 (MQ=12)
  ggAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGa                                                                  <  1:303111/71‑1 (MQ=255)
  ggAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGa                                                                  <  1:1613290/71‑1 (MQ=255)
  ggAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGa                                                                  <  1:5539536/71‑1 (MQ=255)
     gaAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAAc                                                                >  1:5097356/1‑70 (MQ=255)
       aGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCAc                                                             <  1:4487975/71‑1 (MQ=255)
           tACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTaa                                                         >  1:702603/1‑71 (MQ=255)
           tACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTaa                                                         >  1:2964801/1‑71 (MQ=255)
           tACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGt                                                                     >  1:2812273/1‑59 (MQ=255)
           tACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTa                                                          <  1:2181620/70‑1 (MQ=255)
           tACGAACACATCTTTAGAGCCATCGTCAGGAGTGATGAAGCCGAAGCCTTTGTCAGCGTTGAACCAtttta                                                         >  1:4551257/1‑69 (MQ=255)
             cGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTaaa                                                                 >  1:3629146/1‑61 (MQ=9)
             cGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAAc                                                                >  1:2622983/1‑62 (MQ=9)
             cGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAAc                                                                >  1:2731558/1‑62 (MQ=9)
             cGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAAcg                                                       >  1:4127071/1‑70 (MQ=12)
             cGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAAcg                                                       >  1:192844/1‑70 (MQ=12)
             cGAACACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAAc                                                        <  1:3081630/70‑1 (MQ=12)
             cGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAAcc                                                       <  1:4801092/71‑1 (MQ=255)
              gAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCt                                                      >  1:3011678/1‑71 (MQ=255)
              gAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCt                                                      >  1:4944556/1‑71 (MQ=255)
               aaCACGTCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAAcgtt                                                     <  1:4154567/71‑4 (MQ=12)
                acacATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGa                                                    <  1:3624785/71‑1 (MQ=255)
                acacATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGa                                                    <  1:3971008/71‑1 (MQ=255)
                 cacaTCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCAc                                                             <  1:476376/61‑1 (MQ=255)
                 cacaTCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCAc                                                             <  1:3855277/61‑1 (MQ=255)
                   cgtCTTTGCTGCCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAACGTTACCt                                                 >  1:3944241/3‑71 (MQ=12)
                   caTCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCt                                                 <  1:1152790/71‑1 (MQ=255)
                   caTCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCt                                                 <  1:4781149/71‑1 (MQ=255)
                    aTCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCtt                                                <  1:4970200/71‑1 (MQ=255)
                     tCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTg                                                                   <  1:5642545/51‑1 (MQ=255)
                     tCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTa                                                          <  1:4881688/60‑1 (MQ=255)
                      cTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTaa                                                         >  1:5689306/1‑60 (MQ=255)
                       tttGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTg                                                     >  1:5654221/1‑63 (MQ=255)
                       tttGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTaa                                             >  1:4453267/1‑71 (MQ=255)
                         tgctgcCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAACGTTACCTTTaa                                             <  1:3695205/69‑1 (MQ=255)
                         tgctgcCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTa                                              <  1:3678572/68‑1 (MQ=255)
                         tgctgcCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTa                                              <  1:3454643/68‑1 (MQ=255)
                         tgctgcCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTa                                              <  1:2164328/68‑1 (MQ=255)
                          gctgcCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCt                                          >  1:5137044/1‑71 (MQ=255)
                           ctgcCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAACGTTACCTTTAATCtt                                         <  1:5041135/71‑1 (MQ=255)
                           ctgcCGTCTTCCGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAACGTTACCTTTAATCt                                          <  1:3743808/70‑1 (MQ=255)
                            tgcCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTa                                              <  1:402581/65‑1 (MQ=255)
                              ccATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGc                                      >  1:1797797/1‑71 (MQ=255)
                                aTCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCa                                    <  1:5384946/71‑1 (MQ=255)
                                 tCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCt                                          <  1:3575/64‑1 (MQ=255)
                                 tCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCt                                          <  1:2535722/64‑1 (MQ=255)
                                 tCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCAt                                   >  1:5457350/1‑71 (MQ=255)
                                 tCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCAt                                   <  1:1346808/71‑1 (MQ=255)
                                   ttccGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCa                                                              >  1:4018360/3‑42 (MQ=255)
                                   ttccGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCa                                                              >  1:3968665/3‑42 (MQ=255)
                                   aGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTaa                                                         >  1:5227390/1‑47 (MQ=255)
                                     ccGTAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAAc                                                        >  1:3683862/1‑46 (MQ=12)
                                     ccGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTa                                                          <  1:3480573/44‑1 (MQ=255)
                                     ccGGAGTAATGAAACCGAATCCTTTGGACTCATTaaa                                                                 >  1:3854253/1‑37 (MQ=12)
                                     ccGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAAc                                                        >  1:2053871/1‑46 (MQ=16)
                                     ccGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAACGTTACCTTTAATCTTAGACAtcaa                                <  1:3496766/70‑4 (MQ=255)
                                     ccGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAACGTTACCTTTAATCTTAGACAtcaa                                <  1:3476175/70‑4 (MQ=255)
                                     ccGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAACGTTACCTTTAATCTTAGACAtca                                 <  1:3136418/69‑3 (MQ=255)
                                     ccGGAGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAACGTTACCTTTAATCTTAGACAtca                                 <  1:3627065/69‑3 (MQ=255)
                                       ggAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCa                                    <  1:2943115/64‑1 (MQ=255)
                                       ggAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGaaa                             <  1:3067404/71‑1 (MQ=255)
                                       ggAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGaaa                             <  1:3113148/71‑1 (MQ=255)
                                         aGTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAACGTTACCTTTAATCtta                                        >  1:810974/1‑57 (MQ=16)
                                          gTAATGAAACCGAATCCTTTGGACTCATTAAACCACTTAACGTTAcc                                                  >  1:3366984/1‑47 (MQ=9)
                                           tAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCtt                                                <  1:1822953/48‑1 (MQ=255)
                                           tAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCtt                                                <  1:4339395/48‑1 (MQ=255)
                                           tAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGaaa                             <  1:4087591/67‑1 (MQ=255)
                                            aaTGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCttt                                               <  1:3264398/48‑1 (MQ=255)
                                             aTGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTaa                                             >  1:172183/1‑49 (MQ=255)
                                             aTGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTaa                                             >  1:4980506/1‑49 (MQ=255)
                                             aTGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTcc                       <  1:2504374/71‑1 (MQ=255)
                                             aTGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTcc                       <  1:3240707/71‑1 (MQ=255)
                                             aTGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTcc                       >  1:3877377/1‑71 (MQ=255)
                                             aTGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTcc                       >  1:3116939/1‑71 (MQ=255)
                                              tGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCAt                                   <  1:5568382/58‑1 (MQ=255)
                                              tGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCAt                                   <  1:4771719/58‑1 (MQ=255)
                                              tGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCt                      <  1:541882/71‑1 (MQ=255)
                                                aaGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGaaaaa                           >  1:4413746/1‑64 (MQ=255)
                                                aaGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGaaaaa                           >  1:1909686/1‑64 (MQ=255)
                                                aaGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGaaaaa                           >  1:2466670/1‑64 (MQ=255)
                                                aaGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGaaaaa                           >  1:560782/1‑64 (MQ=255)
                                                   ccAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTTcc                                     <  1:2447139/51‑1 (MQ=255)
                                                    cAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGAt                 >  1:2456088/1‑70 (MQ=255)
                                                     aaaaCCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATc                                           <  1:2119062/43‑1 (MQ=255)
                                                     aaaaCCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGcc                                     <  1:3424188/49‑1 (MQ=255)
                                                     aaaaCCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCAt                                   >  1:2056603/1‑51 (MQ=255)
                                                      aaaCCTTTAGACTCGTTGAACCACTTAACCTGACCTTTa                                              <  1:4757809/39‑1 (MQ=255)
                                                       aaCCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGtt             >  1:292420/1‑71 (MQ=255)
                                                       aaCCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGtt             <  1:2991673/71‑1 (MQ=255)
                                                        aCCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAAt                                            >  1:3057925/1‑39 (MQ=255)
                                                          cTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTa                    <  1:455692/61‑1 (MQ=255)
                                                          cTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTa                    <  1:4466759/61‑1 (MQ=255)
                                                          cTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTttc          <  1:1600336/71‑1 (MQ=255)
                                                          cTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTttc          <  1:1593635/71‑1 (MQ=255)
                                                           tttAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTttct         >  1:492893/1‑71 (MQ=255)
                                                            ttAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTttc          >  1:4366700/1‑69 (MQ=255)
                                                                    gTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCg  >  1:5641283/1‑69 (MQ=255)
                                                                    |                                                                    
GCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAGCCAAAACCTTTAGACTCGTTGAACCACTTAACCTGACCTTTAATCTTTGCCATTTGAAAAATTCCTTAGATTGTTTTCTTCGCCCG  >  Exported/1211248‑1211384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: