Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,258,389 T→G 23.6% intergenic (+60/+105) yecP → / ← torZ predicted methyltransferase/trimethylamine N‑oxide reductase system III, catalytic subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,258,3890TG23.6% 156.8 / 49.8 88intergenic (+60/+105)yecP/torZpredicted methyltransferase/trimethylamine N‑oxide reductase system III, catalytic subunit
Reads supporting (aligned to +/- strand):  ref base T (25/37);  new base G (8/10);  total (41/47)
Fisher's exact test for biased strand distribution p-value = 7.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.90e-01

GCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCAT  >  Exported/1258323‑1258458
                                                                  |                                                                     
gCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAAtt                                                                   >  1:4526559/1‑71 (MQ=255)
 ccGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTc                                                                  <  1:1501724/71‑1 (MQ=255)
   gTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAAtt                                                                   <  1:927460/68‑1 (MQ=255)
   gTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCaa                                                                <  1:5475036/71‑1 (MQ=255)
    tAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCaa                                                                >  1:412870/1‑70 (MQ=255)
      aaGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAatat                                                             <  1:5024919/71‑1 (MQ=255)
      aaGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAata                                                              >  1:2229955/1‑70 (MQ=255)
      aaGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAata                                                              >  1:2076606/1‑70 (MQ=255)
        ggTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAatatat                                                           >  1:3478701/1‑71 (MQ=255)
          tCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTg                                                         <  1:1485836/71‑1 (MQ=255)
          tCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTg                                                         <  1:4844379/71‑1 (MQ=255)
            tGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTg                                                         >  1:3480889/1‑69 (MQ=255)
              gTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAgg                                                     <  1:4004945/71‑1 (MQ=255)
                 ttACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAAtt                                                                   <  1:2476714/54‑1 (MQ=255)
                 ttACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAata                                                              <  1:3528017/59‑1 (MQ=255)
                 ttACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGAc                                                  >  1:4767619/1‑71 (MQ=255)
                   aCTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAata                                                              <  1:1564739/57‑1 (MQ=255)
                     tGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAata                                                              <  1:5606571/55‑1 (MQ=255)
                       ccGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATg                                              >  1:3734384/1‑69 (MQ=255)
                       ccGGATGCGGCGTGAACGCCTTATCCGACCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATg                                              <  1:4526893/69‑1 (MQ=255)
                        cGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCa                                                                 <  1:1022153/49‑1 (MQ=255)
                        cGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCa                                                                 <  1:857020/49‑1 (MQ=255)
                         ggATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAgg                                          <  1:3870347/71‑1 (MQ=255)
                          gATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAAtt                                                                   <  1:2538819/45‑1 (MQ=255)
                           aTGCGGCGTGAACGCCTTATCCGACCCACAAAGTCTTGCAAAATCAATAAATTGCAGGAACGATGTAGGcc                                        >  1:1312535/1‑71 (MQ=2)
                            tGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAata                                                              >  1:887837/1‑48 (MQ=255)
                              cggcgTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTg                                      <  1:3147214/70‑1 (MQ=255)
                                gcgTGAACGCCTTATCCGGCCTACAAATCCTTGCGAATTCAata                                                              <  1:5157068/44‑1 (MQ=11)
                                gcgTGAACGCCTTATCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGAAATTCAGGCCTGATaa                                  <  1:3969092/71‑1 (MQ=2)
                                 cgTGAACGCCTTATCCGGCCTACAAATCCTTGCGAATTCAata                                                              <  1:2385923/43‑1 (MQ=14)
                                 cgTGAACGCCTTATCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGAAATTCAGGCCTGATAAg                                 >  1:2912678/1‑71 (MQ=9)
                                 cgTGAACGCCTTATCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGAAATTCAGGCCTGATAAg                                 <  1:163709/71‑1 (MQ=9)
                                 cgTGAACGCCTTATCCGACCCACAAAGTCTTGCAAAATCAATAAATTGCAGGAACGATGTAGGCCTGAt                                    >  1:1196526/1‑69 (MQ=2)
                                 cgTGAACGCCTTATCCGACCCACAAAGTCTTGCAAAATCAATAAATTGCAGGAACGATGTAGGCCTGAt                                    >  1:5601232/1‑69 (MQ=2)
                                  gTGAACGCCTTATCCGGCCTACAAATCCTTGCGAATTCAatata                                                            >  1:2725210/1‑44 (MQ=11)
                                  gTGAACGCCTTATCCGACCCACAAAGTCTTGCAAAATCAATAAATTGCAGGAACGATGTAgg                                          >  1:88908/1‑62 (MQ=2)
                                  gTGAACGCCTTATCCGACCCACAAAGTCTTGCAAAATCAATAAATTGCAGGAACGATGTAgg                                          >  1:4938926/1‑62 (MQ=2)
                                   tGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCa                                                       <  1:2533891/48‑1 (MQ=255)
                                   tGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATg                                              <  1:3963067/57‑1 (MQ=255)
                                     aaCGCCTTATCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGAAATTCAGGCCTGATAAGcg                               >  1:5304301/1‑68 (MQ=9)
                                     aaCGCCTTATCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGAAATTCAGGCCTGATAAGcg                               >  1:1237008/1‑68 (MQ=9)
                                     aaCGCCTTATCCGGCCTACAAAACCTTGCTAATTCaa                                                                >  1:4045185/1‑37 (MQ=255)
                                     aaCGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAgg                                                     >  1:4206099/1‑48 (MQ=255)
                                     aaCGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAgg                                                     >  1:5130665/1‑48 (MQ=255)
                                     aaCGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAgg                                                     <  1:3962078/48‑1 (MQ=255)
                                     aaCGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCAt                              <  1:4433128/71‑1 (MQ=255)
                                     aaCGCCTTATCCGACCCACAAAGTCTTGCAAAATCAATAAATTGCAGGAACGATGTAGGCCTGATAAGcg                               >  1:1345539/1‑69 (MQ=2)
                                      aCGCCTTATCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGAAATTCAGGCCTGATAAGCGTAg                            >  1:4359043/1‑71 (MQ=9)
                                      aCGCCTTATCCGACCCACAAAGTCTTGCAAAATCAATAAATTGCAGGAACGATGTAGGCCTGATAAGcg                               >  1:4030252/1‑68 (MQ=2)
                                       cGCCTTATCCGGCCTACAAATCCTTGCGAATTCAata                                                              <  1:5378064/37‑1 (MQ=14)
                                       cGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGa                                                   >  1:106423/1‑48 (MQ=255)
                                       cGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGa                                                   >  1:5654816/1‑48 (MQ=255)
                                         ccTTATCCGGCCTACAAATCCTTGCGAATTCAata                                                              >  1:3253930/1‑35 (MQ=14)
                                         ccTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAgcg                          >  1:1787019/1‑71 (MQ=255)
                                         ccTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAgcg                          >  1:3335205/1‑71 (MQ=255)
                                           ttATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAgcg                          <  1:955393/69‑1 (MQ=255)
                                           ttATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAgcg                          <  1:63454/69‑1 (MQ=255)
                                           ttATCCGACCCACAAAGTCTTGCAAAATCAATAAATAGCAGGAACGATGTAGGCCTGATa                                   >  1:935178/1‑60 (MQ=2)
                                             aTCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGAAATTCAGGCCTGATAAGCGTAGCGCAt                       >  1:1791272/1‑69 (MQ=9)
                                             aTCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCa                        >  1:4474839/1‑69 (MQ=255)
                                             aTCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCAt                       >  1:4121435/1‑70 (MQ=255)
                                             aTCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCAt                       >  1:5182346/1‑70 (MQ=255)
                                              tCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGaaa                                                 <  1:144553/43‑3 (MQ=14)
                                              tCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGaaa                                                 <  1:3417786/43‑3 (MQ=14)
                                              tCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGaaa                                                 <  1:4037079/43‑3 (MQ=14)
                                              tCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGaaa                                                 <  1:3424206/43‑3 (MQ=14)
                                              tCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGAAATTCAGGCCTGATAAGCGTAGCGCATCAg                    <  1:4437046/71‑1 (MQ=9)
                                                  gCCTACAAATCCTTGCGAATTCAATATATTGTATGGAAATTCAGGCCTGATAAGCGTAGCGCATCa                     >  1:3288905/1‑66 (MQ=9)
                                                  gCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAg                                 <  1:3345577/55‑1 (MQ=255)
                                                  gCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAg                                 <  1:920956/55‑1 (MQ=255)
                                                     tACAAAACCTTGCTAATTCAATATATTGCAGGGACTAt                                               <  1:4967192/38‑1 (MQ=255)
                                                     tACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCa                        <  1:2978919/61‑1 (MQ=255)
                                                      aCAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCAt                              <  1:1561115/54‑1 (MQ=255)
                                                      aCAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCtt             >  1:4506173/1‑71 (MQ=255)
                                                       cAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCt                                       <  1:3539309/44‑1 (MQ=255)
                                                       cAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCt                                       <  1:2390062/44‑1 (MQ=255)
                                                        aaaaCCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCAt                       <  1:959986/59‑1 (MQ=255)
                                                        aaaaCCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCAt                       <  1:3053980/59‑1 (MQ=255)
                                                        aaaaCCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCttt            <  1:1847400/70‑1 (MQ=255)
                                                          aaCCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGc                                >  1:1623060/1‑48 (MQ=255)
                                                           aCCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGt        <  1:4203399/71‑1 (MQ=255)
                                                           aCCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGt        <  1:2336628/71‑1 (MQ=255)
                                                            ccTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGtt       >  1:1082953/1‑71 (MQ=255)
                                                               tGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGttt      >  1:1692566/1‑69 (MQ=255)
                                                                gCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAgg                   >  1:3310184/1‑55 (MQ=255)
                                                                gCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAgg                   >  1:2003473/1‑55 (MQ=255)
                                                                 cTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCAt  <  1:3921158/71‑1 (MQ=255)
                                                                 cTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCAt  <  1:3633435/71‑1 (MQ=255)
                                                                  tAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCAt  >  1:3643659/1‑70 (MQ=255)
                                                                  tAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCAt  >  1:2458677/1‑70 (MQ=255)
                                                                  |                                                                     
GCCGTAAAGGTCTGGTATTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCAT  >  Exported/1258323‑1258458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: