Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,258,409 A→G 16.1% intergenic (+80/+85) yecP → / ← torZ predicted methyltransferase/trimethylamine N‑oxide reductase system III, catalytic subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,258,4090AG16.1% 180.2 / 27.9 87intergenic (+80/+85)yecP/torZpredicted methyltransferase/trimethylamine N‑oxide reductase system III, catalytic subunit
Reads supporting (aligned to +/- strand):  ref base A (40/33);  new base G (3/11);  total (43/44)
Fisher's exact test for biased strand distribution p-value = 3.85e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

TTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAGCGCCCGTTTC  >  Exported/1258340‑1258476
                                                                     |                                                                   
ttACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGAc                                                                    >  1:4767619/1‑71 (MQ=255)
      ccGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATg                                                                >  1:3734384/1‑69 (MQ=255)
      ccGGATGCGGCGTGAACGCCTTATCCGACCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATg                                                                <  1:4526893/69‑1 (MQ=255)
        ggATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAgg                                                            <  1:3870347/71‑1 (MQ=255)
          aTGCGGCGTGAACGCCTTATCCGACCCACAAAGTCTTGCAAAATCAATAAATTGCAGGAACGATGTAGGcc                                                          >  1:1312535/1‑71 (MQ=2)
             cggcgTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTg                                                        <  1:3147214/70‑1 (MQ=255)
               gcgTGAACGCCTTATCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGAAATTCAGGCCTGATaa                                                    <  1:3969092/71‑1 (MQ=2)
                cgTGAACGCCTTATCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGAAATTCAGGCCTGATAAg                                                   >  1:2912678/1‑71 (MQ=9)
                cgTGAACGCCTTATCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGAAATTCAGGCCTGATAAg                                                   <  1:163709/71‑1 (MQ=9)
                cgTGAACGCCTTATCCGACCCACAAAGTCTTGCAAAATCAATAAATTGCAGGAACGATGTAGGCCTGAt                                                      >  1:1196526/1‑69 (MQ=2)
                cgTGAACGCCTTATCCGACCCACAAAGTCTTGCAAAATCAATAAATTGCAGGAACGATGTAGGCCTGAt                                                      >  1:5601232/1‑69 (MQ=2)
                 gTGAACGCCTTATCCGACCCACAAAGTCTTGCAAAATCAATAAATTGCAGGAACGATGTAgg                                                            >  1:4938926/1‑62 (MQ=2)
                 gTGAACGCCTTATCCGACCCACAAAGTCTTGCAAAATCAATAAATTGCAGGAACGATGTAgg                                                            >  1:88908/1‑62 (MQ=2)
                  tGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATg                                                                <  1:3963067/57‑1 (MQ=255)
                    aaCGCCTTATCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGAAATTCAGGCCTGATAAGcg                                                 >  1:1237008/1‑68 (MQ=9)
                    aaCGCCTTATCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGAAATTCAGGCCTGATAAGcg                                                 >  1:5304301/1‑68 (MQ=9)
                    aaCGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCAt                                                <  1:4433128/71‑1 (MQ=255)
                    aaCGCCTTATCCGACCCACAAAGTCTTGCAAAATCAATAAATTGCAGGAACGATGTAGGCCTGATAAGcg                                                 >  1:1345539/1‑69 (MQ=2)
                     aCGCCTTATCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGAAATTCAGGCCTGATAAGCGTAg                                              >  1:4359043/1‑71 (MQ=9)
                     aCGCCTTATCCGACCCACAAAGTCTTGCAAAATCAATAAATTGCAGGAACGATGTAGGCCTGATAAGcg                                                 >  1:4030252/1‑68 (MQ=2)
                      cGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGa                                                                     >  1:106423/1‑48 (MQ=255)
                      cGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGa                                                                     >  1:5654816/1‑48 (MQ=255)
                        ccTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAgcg                                            >  1:1787019/1‑71 (MQ=255)
                        ccTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAgcg                                            >  1:3335205/1‑71 (MQ=255)
                          ttATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAgcg                                            <  1:955393/69‑1 (MQ=255)
                          ttATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAgcg                                            <  1:63454/69‑1 (MQ=255)
                          ttATCCGACCCACAAAGTCTTGCAAAATCAATAAATAGCAGGAACGATGTAGGCCTGATa                                                     >  1:935178/1‑60 (MQ=2)
                            aTCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGAAATTCAGGCCTGATAAGCGTAGCGCAt                                         >  1:1791272/1‑69 (MQ=9)
                            aTCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCa                                          >  1:4474839/1‑69 (MQ=255)
                            aTCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCAt                                         >  1:5182346/1‑70 (MQ=255)
                            aTCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCAt                                         >  1:4121435/1‑70 (MQ=255)
                             tCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGaaa                                                                   <  1:3424206/43‑3 (MQ=14)
                             tCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGaaa                                                                   <  1:3417786/43‑3 (MQ=14)
                             tCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGaaa                                                                   <  1:4037079/43‑3 (MQ=14)
                             tCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGaaa                                                                   <  1:144553/43‑3 (MQ=14)
                             tCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGAAATTCAGGCCTGATAAGCGTAGCGCATCAg                                      <  1:4437046/71‑1 (MQ=9)
                                 gCCTACAAATCCTTGCGAATTCAATATATTGTATGGAAATTCAGGCCTGATAAGCGTAGCGCATCa                                       >  1:3288905/1‑66 (MQ=9)
                                 gCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAg                                                   <  1:920956/55‑1 (MQ=255)
                                 gCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAg                                                   <  1:3345577/55‑1 (MQ=255)
                                    tACAAAACCTTGCTAATTCAATATATTGCAGGGACTAt                                                                 <  1:4967192/38‑1 (MQ=255)
                                    tACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCa                                          <  1:2978919/61‑1 (MQ=255)
                                     aCAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCAt                                                <  1:1561115/54‑1 (MQ=255)
                                     aCAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCtt                               >  1:4506173/1‑71 (MQ=255)
                                      cAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCt                                                         <  1:2390062/44‑1 (MQ=255)
                                      cAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCt                                                         <  1:3539309/44‑1 (MQ=255)
                                       aaaaCCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCAt                                         <  1:959986/59‑1 (MQ=255)
                                       aaaaCCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCAt                                         <  1:3053980/59‑1 (MQ=255)
                                       aaaaCCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCttt                              <  1:1847400/70‑1 (MQ=255)
                                         aaCCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGc                                                  >  1:1623060/1‑48 (MQ=255)
                                          aCCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGt                          <  1:4203399/71‑1 (MQ=255)
                                          aCCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGt                          <  1:2336628/71‑1 (MQ=255)
                                           ccTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGtt                         >  1:1082953/1‑71 (MQ=255)
                                              tGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGttt                        >  1:1692566/1‑69 (MQ=255)
                                               gCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAgg                                     >  1:3310184/1‑55 (MQ=255)
                                               gCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAgg                                     >  1:2003473/1‑55 (MQ=255)
                                                cTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCAt                    <  1:3633435/71‑1 (MQ=255)
                                                cTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCAt                    <  1:3921158/71‑1 (MQ=255)
                                                 tAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCAt                    >  1:2458677/1‑70 (MQ=255)
                                                 tAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCAt                    >  1:3643659/1‑70 (MQ=255)
                                                 aagttCAATATATTTAATGGGTTATGTAGACCTGATAAGCATAGCGCATCAGGCTGCTTTGCGttt                        >  1:2317224/4‑66 (MQ=9)
                                                  aaTTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCtt                               >  1:3091987/1‑58 (MQ=255)
                                                  aaTTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCtt                               >  1:831128/1‑58 (MQ=255)
                                                   aTTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATaa                  >  1:4601760/1‑70 (MQ=255)
                                                    ttCAATATATTTAATGGGTTATGTAGACCTGATAAGCATAGCGCATCAGGctg                                  <  1:5610084/53‑3 (MQ=9)
                                                    ttCAATATATTTAATGGGTTATGTAGACCTGATAAGCATAGCGCATCAGGctg                                  <  1:4798301/53‑3 (MQ=9)
                                                    ttCAATATATTTAATGGGTTATGTAGACCTGATAAGCATAGCGCATCAGGctg                                  <  1:4791809/53‑3 (MQ=9)
                                                    ttCAATATATTTAATGGGTTATGTAGACCTGATAAGCATAGCGCATCAGGctg                                  <  1:1985576/53‑3 (MQ=9)
                                                    ttCAATATATTTAATGGGTTATGTAGACCTGATAAGCATAGCGCATCAGGCTGCtt                               <  1:1378907/56‑1 (MQ=9)
                                                    ttCAATATATTTAATGGGTTATGTAGACCTGATAAGCATAGCGCATCAGGCTGCtt                               <  1:5190587/56‑1 (MQ=9)
                                                    ttCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCa                                          <  1:2500841/45‑1 (MQ=255)
                                                     tCAATATATTTAATGGGTTATGTAGACCTGATAAGCATAGCGCATCAGgctgct                                <  1:2949309/54‑1 (MQ=9)
                                                      cAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACg                           >  1:1236330/1‑58 (MQ=255)
                                                      cAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCt               <  1:943738/70‑1 (MQ=255)
                                                       aaTATATTTAATGGGTTATGTAGACCTGATAAGCATAgcgc                                           <  1:5605311/41‑1 (MQ=12)
                                                       aaTATATTTAATGGGTTATGTAGACCTGATAAGCATAgcgc                                           <  1:5136185/41‑1 (MQ=12)
                                                       aaTATATTTAATGGGTTATGTAGACCTGATAAGCATAgcgc                                           <  1:5038193/41‑1 (MQ=12)
                                                       aaTATATTTAATGGGTTATGTAGACCTGATAAGCATAgcgc                                           <  1:3025246/41‑1 (MQ=12)
                                                       aaTATATTTAATGGGTTATGTAGACCTGATAAGCATAGCGCATCAGGCTGCTTTGCGt                          >  1:1525217/1‑58 (MQ=9)
                                                       aaTATATTTAATGGGTTATGTAGACCTGATAAGCATAGCGCATCAGGCTGCTTTGCGt                          >  1:3009687/1‑58 (MQ=9)
                                                        atatatTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAg            <  1:357589/71‑1 (MQ=255)
                                                        atatatTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAg            <  1:2701218/71‑1 (MQ=255)
                                                         tatatTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCAt                                         <  1:785787/41‑1 (MQ=255)
                                                         tatatTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCa             >  1:948591/1‑69 (MQ=255)
                                                          atatTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCa                                       >  1:913594/1‑42 (MQ=255)
                                                           tatTGGAGGGACTATGTAGGCCTGATAAGCATAGCGCATc                                        >  1:3183619/1‑40 (MQ=255)
                                                           tatTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATc                                        >  1:5316275/1‑40 (MQ=255)
                                                           tatTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATc                                        >  1:618650/1‑40 (MQ=255)
                                                           tatTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATc                                        >  1:2292898/1‑40 (MQ=255)
                                                            atTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAgcgc         <  1:3789462/70‑1 (MQ=255)
                                                             ttGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAGCGccc       >  1:5067378/1‑71 (MQ=255)
                                                                cAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAGCGCCCGtt    <  1:3583970/71‑1 (MQ=255)
                                                                  gggACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAGCGCCCGTTTc  <  1:1467958/71‑1 (MQ=255)
                                                                   ggACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTg                       <  1:2382131/49‑1 (MQ=255)
                                                                     |                                                                   
TTACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAGCGCCCGTTTC  >  Exported/1258340‑1258476

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: