Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,258,415 T→C 9.8% intergenic (+86/+79) yecP → / ← torZ predicted methyltransferase/trimethylamine N‑oxide reductase system III, catalytic subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,258,4150TC 9.8% 204.5 / 13.6 92intergenic (+86/+79)yecP/torZpredicted methyltransferase/trimethylamine N‑oxide reductase system III, catalytic subunit
Reads supporting (aligned to +/- strand):  ref base T (35/48);  new base C (6/3);  total (41/51)
Fisher's exact test for biased strand distribution p-value = 1.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

GGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAGCGCCCGTTTCCGGGCGC  >  Exported/1258348‑1258483
                                                                   |                                                                    
ggATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAgg                                                                   <  1:3870347/71‑1 (MQ=255)
  aTGCGGCGTGAACGCCTTATCCGACCCACAAAGTCTTGCAAAATCAATAAATTGCAGGAACGATGTAGGcc                                                                 >  1:1312535/1‑71 (MQ=2)
     cggcgTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTg                                                               <  1:3147214/70‑1 (MQ=255)
       gcgTGAACGCCTTATCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGAAATTCAGGCCTGATaa                                                           <  1:3969092/71‑1 (MQ=2)
        cgTGAACGCCTTATCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGAAATTCAGGCCTGATAAg                                                          >  1:2912678/1‑71 (MQ=9)
        cgTGAACGCCTTATCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGAAATTCAGGCCTGATAAg                                                          <  1:163709/71‑1 (MQ=9)
        cgTGAACGCCTTATCCGACCCACAAAGTCTTGCAAAATCAATAAATTGCAGGAACGATGTAGGCCTGAt                                                             >  1:1196526/1‑69 (MQ=2)
        cgTGAACGCCTTATCCGACCCACAAAGTCTTGCAAAATCAATAAATTGCAGGAACGATGTAGGCCTGAt                                                             >  1:5601232/1‑69 (MQ=2)
         gTGAACGCCTTATCCGACCCACAAAGTCTTGCAAAATCAATAAATTGCAGGAACGATGTAgg                                                                   >  1:88908/1‑62 (MQ=2)
         gTGAACGCCTTATCCGACCCACAAAGTCTTGCAAAATCAATAAATTGCAGGAACGATGTAgg                                                                   >  1:4938926/1‑62 (MQ=2)
            aaCGCCTTATCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGAAATTCAGGCCTGATAAGcg                                                        >  1:1237008/1‑68 (MQ=9)
            aaCGCCTTATCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGAAATTCAGGCCTGATAAGcg                                                        >  1:5304301/1‑68 (MQ=9)
            aaCGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCAt                                                       <  1:4433128/71‑1 (MQ=255)
            aaCGCCTTATCCGACCCACAAAGTCTTGCAAAATCAATAAATTGCAGGAACGATGTAGGCCTGATAAGcg                                                        >  1:1345539/1‑69 (MQ=2)
             aCGCCTTATCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGAAATTCAGGCCTGATAAGCGTAg                                                     >  1:4359043/1‑71 (MQ=9)
             aCGCCTTATCCGACCCACAAAGTCTTGCAAAATCAATAAATTGCAGGAACGATGTAGGCCTGATAAGcg                                                        >  1:4030252/1‑68 (MQ=2)
                ccTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAgcg                                                   >  1:1787019/1‑71 (MQ=255)
                ccTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAgcg                                                   >  1:3335205/1‑71 (MQ=255)
                  ttATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAgcg                                                   <  1:955393/69‑1 (MQ=255)
                  ttATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAgcg                                                   <  1:63454/69‑1 (MQ=255)
                  ttATCCGACCCACAAAGTCTTGCAAAATCAATAAATAGCAGGAACGATGTAGGCCTGATa                                                            >  1:935178/1‑60 (MQ=2)
                    aTCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGAAATTCAGGCCTGATAAGCGTAGCGCAt                                                >  1:1791272/1‑69 (MQ=9)
                    aTCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCa                                                 >  1:4474839/1‑69 (MQ=255)
                    aTCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCAt                                                >  1:5182346/1‑70 (MQ=255)
                    aTCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCAt                                                >  1:4121435/1‑70 (MQ=255)
                     tCCGGCCTACAAATCCTTGCGAATTCAATATATTGTATGGAAATTCAGGCCTGATAAGCGTAGCGCATCAg                                             <  1:4437046/71‑1 (MQ=9)
                         gCCTACAAATCCTTGCGAATTCAATATATTGTATGGAAATTCAGGCCTGATAAGCGTAGCGCATCa                                              >  1:3288905/1‑66 (MQ=9)
                         gCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAg                                                          <  1:920956/55‑1 (MQ=255)
                         gCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAg                                                          <  1:3345577/55‑1 (MQ=255)
                            tACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCa                                                 <  1:2978919/61‑1 (MQ=255)
                             aCAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCAt                                                       <  1:1561115/54‑1 (MQ=255)
                             aCAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCtt                                      >  1:4506173/1‑71 (MQ=255)
                              cAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCt                                                                <  1:3539309/44‑1 (MQ=255)
                              cAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCt                                                                <  1:2390062/44‑1 (MQ=255)
                               aaaaCCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCAt                                                <  1:959986/59‑1 (MQ=255)
                               aaaaCCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCAt                                                <  1:3053980/59‑1 (MQ=255)
                               aaaaCCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCttt                                     <  1:1847400/70‑1 (MQ=255)
                                 aaCCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGc                                                         >  1:1623060/1‑48 (MQ=255)
                                  aCCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGt                                 <  1:4203399/71‑1 (MQ=255)
                                  aCCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGt                                 <  1:2336628/71‑1 (MQ=255)
                                   ccTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGtt                                >  1:1082953/1‑71 (MQ=255)
                                      tGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGttt                               >  1:1692566/1‑69 (MQ=255)
                                       gCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAgg                                            >  1:3310184/1‑55 (MQ=255)
                                       gCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAgg                                            >  1:2003473/1‑55 (MQ=255)
                                        cTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCAt                           <  1:3633435/71‑1 (MQ=255)
                                        cTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCAt                           <  1:3921158/71‑1 (MQ=255)
                                         tAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCAt                           >  1:3643659/1‑70 (MQ=255)
                                         tAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCAt                           >  1:2458677/1‑70 (MQ=255)
                                         aagttCAATATATTTAATGGGTTATGTAGACCTGATAAGCATAGCGCATCAGGCTGCTTTGCGttt                               >  1:2317224/4‑66 (MQ=9)
                                          aaTTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCtt                                      >  1:3091987/1‑58 (MQ=255)
                                          aaTTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCtt                                      >  1:831128/1‑58 (MQ=255)
                                           aTTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATaa                         >  1:4601760/1‑70 (MQ=255)
                                            ttCAATATATTTAATGGGTTATGTAGACCTGATAAGCATAGCGCATCAGGctg                                         <  1:5610084/53‑3 (MQ=9)
                                            ttCAATATATTTAATGGGTTATGTAGACCTGATAAGCATAGCGCATCAGGctg                                         <  1:1985576/53‑3 (MQ=9)
                                            ttCAATATATTTAATGGGTTATGTAGACCTGATAAGCATAGCGCATCAGGctg                                         <  1:4798301/53‑3 (MQ=9)
                                            ttCAATATATTTAATGGGTTATGTAGACCTGATAAGCATAGCGCATCAGGctg                                         <  1:4791809/53‑3 (MQ=9)
                                            ttCAATATATTTAATGGGTTATGTAGACCTGATAAGCATAGCGCATCAGGCTGCtt                                      <  1:1378907/56‑1 (MQ=9)
                                            ttCAATATATTTAATGGGTTATGTAGACCTGATAAGCATAGCGCATCAGGCTGCtt                                      <  1:5190587/56‑1 (MQ=9)
                                            ttCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCa                                                 <  1:2500841/45‑1 (MQ=255)
                                             tCAATATATTTAATGGGTTATGTAGACCTGATAAGCATAGCGCATCAGgctgct                                       <  1:2949309/54‑1 (MQ=9)
                                              cAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACg                                  >  1:1236330/1‑58 (MQ=255)
                                              cAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCt                      <  1:943738/70‑1 (MQ=255)
                                               aaTATATTTAATGGGTTATGTAGACCTGATAAGCATAgcgc                                                  <  1:3025246/41‑1 (MQ=12)
                                               aaTATATTTAATGGGTTATGTAGACCTGATAAGCATAgcgc                                                  <  1:5605311/41‑1 (MQ=12)
                                               aaTATATTTAATGGGTTATGTAGACCTGATAAGCATAgcgc                                                  <  1:5136185/41‑1 (MQ=12)
                                               aaTATATTTAATGGGTTATGTAGACCTGATAAGCATAgcgc                                                  <  1:5038193/41‑1 (MQ=12)
                                               aaTATATTTAATGGGTTATGTAGACCTGATAAGCATAGCGCATCAGGCTGCTTTGCGt                                 >  1:3009687/1‑58 (MQ=9)
                                               aaTATATTTAATGGGTTATGTAGACCTGATAAGCATAGCGCATCAGGCTGCTTTGCGt                                 >  1:1525217/1‑58 (MQ=9)
                                                atatatTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAg                   <  1:2701218/71‑1 (MQ=255)
                                                atatatTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAg                   <  1:357589/71‑1 (MQ=255)
                                                 tatatTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCAt                                                <  1:785787/41‑1 (MQ=255)
                                                 tatatTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCa                    >  1:948591/1‑69 (MQ=255)
                                                  atatTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCa                                              >  1:913594/1‑42 (MQ=255)
                                                   tatTGGAGGGACTATGTAGGCCTGATAAGCATAGCGCATc                                               >  1:3183619/1‑40 (MQ=255)
                                                   tatTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATc                                               >  1:2292898/1‑40 (MQ=255)
                                                   tatTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATc                                               >  1:5316275/1‑40 (MQ=255)
                                                   tatTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATc                                               >  1:618650/1‑40 (MQ=255)
                                                    atTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAgcgc                <  1:3789462/70‑1 (MQ=255)
                                                     ttGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAGCGccc              >  1:5067378/1‑71 (MQ=255)
                                                        cAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAGCGCCCGtt           <  1:3583970/71‑1 (MQ=255)
                                                          gggACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAGCGCCCGTTTc         <  1:1467958/71‑1 (MQ=255)
                                                           ggACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTg                              <  1:2382131/49‑1 (MQ=255)
                                                               tATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAgc                  <  1:5310211/57‑1 (MQ=255)
                                                                aTGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTa                                    <  1:2472096/38‑1 (MQ=255)
                                                                 tGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAGCGCCCGTTTCCGGgcgc  <  1:4297408/71‑1 (MQ=255)
                                                                 tGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAGCGCCCGTTTCCGGgc    <  1:1599104/69‑1 (MQ=255)
                                                                 tGTAGACCTGATAAGCATAGCGCATCAGGCTGCTTTGCg                                  <  1:2256842/39‑1 (MQ=11)
                                                                 tGTAGACCTGATAAGCATAGCGCATCAGGCTGCTTTGCg                                  <  1:1362744/39‑1 (MQ=11)
                                                                 tGTAGACCTGATAAGCATAGCGCATCAGGCTGCTTTGCg                                  <  1:2115693/39‑1 (MQ=11)
                                                                 tGTAGACCTGATAAGCATAGCGCATCAGGCTGCTTTGCg                                  <  1:4712131/39‑1 (MQ=11)
                                                                  gTAGACCTGATAAGCATAGCGCATCAGGCTGCTTTGCGttt                               <  1:420357/41‑1 (MQ=14)
                                                                  gTAGACCTGATAAGCATAGCGCATCAGGCTGCTTTGCGttt                               <  1:5264968/41‑1 (MQ=14)
                                                                   |                                                                    
GGATGCGGCGTGAACGCCTTATCCGGCCTACAAAACCTTGCTAATTCAATATATTGCAGGGACTATGTAGGCCTGATAAGCATAGCGCATCAGGCAGCTTTACGTTTGCATAACCTCAGCGCCCGTTTCCGGGCGC  >  Exported/1258348‑1258483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: