Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,259,304 G→A 16.3% N540N (AAC→AAT torZ ← trimethylamine N‑oxide reductase system III, catalytic subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,259,3040GA16.3% 241.0 / 33.1 104N540N (AAC→AATtorZtrimethylamine N‑oxide reductase system III, catalytic subunit
Reads supporting (aligned to +/- strand):  ref base G (51/36);  new base A (5/12);  total (56/48)
Fisher's exact test for biased strand distribution p-value = 3.44e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 109 total aligned reads displayed.

TACCTTCGGTATAGATCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGG  >  Exported/1259234‑1259374
                                                                      |                                                                      
tACCTTCGGTATAGATCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCg                                                                        >  1:768516/1‑71 (MQ=255)
    ttCGGTATAGATCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTa                                                                     >  1:4333271/1‑70 (MQ=255)
    ttCGGTATAGATCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTa                                                                     >  1:2577817/1‑70 (MQ=255)
      cGGTATAGATCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGt                                                                       <  1:1015638/66‑1 (MQ=255)
       ggTATAGATCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTAcgcg                                                                 <  1:2240435/71‑1 (MQ=255)
        gTATAGATCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGt                                                                       <  1:1451785/64‑1 (MQ=255)
             gATCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCa                                                            <  1:1482188/70‑1 (MQ=255)
             gATCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCa                                                            >  1:1489408/1‑70 (MQ=255)
             gATCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATAGGCAAACACGTCAAAATCGTTACGCGCTTCaa                                                           <  1:1294401/71‑1 (MQ=255)
              aTCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGt                                                                       <  1:3570043/58‑1 (MQ=255)
              aTCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCaaa                                                          >  1:5377354/1‑71 (MQ=255)
              aTCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCaaa                                                          >  1:5384102/1‑71 (MQ=255)
              aTCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCaaa                                                          >  1:2502103/1‑71 (MQ=255)
                ctctTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAAtt                                                        >  1:5305766/1‑71 (MQ=255)
                 tctTTTCCGCCAGGTTTGAGTAATTCTGCAAGATCGGCAAACACGTCAAAATCGt                                                                       <  1:2733187/55‑1 (MQ=255)
                 tctTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGt                                                                       <  1:1802551/55‑1 (MQ=255)
                  ctTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTg                                                       >  1:3528852/1‑70 (MQ=255)
                  ctTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGc                                                      >  1:226675/1‑71 (MQ=255)
                   ttttCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTg                                                       <  1:1766721/69‑1 (MQ=255)
                   ttttCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCg                                                     >  1:3646290/1‑71 (MQ=255)
                   ttttCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCg                                                     >  1:4219728/1‑71 (MQ=255)
                   ttttCCGCCAGGTTTGAGTAAATCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCg                                                     >  1:134994/1‑71 (MQ=255)
                    tttCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGt                                                                       <  1:391081/52‑1 (MQ=255)
                     ttCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCg                                                     >  1:956112/1‑69 (MQ=255)
                      tCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTa                                                                     >  1:1767400/1‑52 (MQ=255)
                      tCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTa                                                                     >  1:4531876/1‑52 (MQ=255)
                         gCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCaa                                               <  1:3140806/71‑1 (MQ=255)
                          ccAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAAtt                                                        <  1:5290847/61‑1 (MQ=255)
                            aGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAg                                            >  1:4979884/1‑71 (MQ=255)
                             ggTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGc                                           >  1:4241530/1‑71 (MQ=255)
                              gTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCa                                                            <  1:5367134/53‑1 (MQ=255)
                              gTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGcc                                          <  1:3677550/71‑1 (MQ=255)
                              gTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGcc                                          <  1:861176/71‑1 (MQ=255)
                              gTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGcc                                          <  1:3357967/71‑1 (MQ=255)
                                ttGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCaaa                                                          >  1:4569347/1‑53 (MQ=255)
                                    gTAATTCCGCAAGATCGGCAAACACGTCAAAATCGtt                                                                      >  1:4067248/1‑37 (MQ=255)
                                     tAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCa                                                            <  1:1722986/46‑1 (MQ=255)
                                     tAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGcc                                          >  1:2259436/1‑64 (MQ=255)
                                     tAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCtt                                     <  1:5173178/69‑1 (MQ=255)
                                     tAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCtt                                     <  1:618322/69‑1 (MQ=255)
                                      aaTTCCGCAAGATCGGCAAACACGTCAAAATCGTTACTCGCTTCAAAtt                                                        <  1:5272599/49‑1 (MQ=255)
                                      aaTTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAAtt                                                        <  1:5293583/49‑1 (MQ=255)
                                      aaTTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAAtt                                                        <  1:3670806/49‑1 (MQ=255)
                                       aTTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCaaa                                                          >  1:4725685/1‑46 (MQ=255)
                                       aTTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCaaa                                                          >  1:1044371/1‑46 (MQ=255)
                                       aTTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCaaa                                                          >  1:413265/1‑46 (MQ=255)
                                       aTTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCaaa                                                          >  1:5453428/1‑46 (MQ=255)
                                       aTTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATc                                 >  1:946461/1‑71 (MQ=255)
                                       aTTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATc                                 >  1:4216608/1‑71 (MQ=255)
                                        ttCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGcc                                          <  1:4448310/61‑1 (MQ=255)
                                        ttCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGcc                                          <  1:2233876/61‑1 (MQ=255)
                                         tCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCgg                               >  1:2372971/1‑71 (MQ=255)
                                          ccGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCaaa                                                          >  1:3546744/1‑43 (MQ=255)
                                          ccGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCaaa                                                          >  1:2127068/1‑43 (MQ=255)
                                          ccGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCaaa                                                          >  1:2940991/1‑43 (MQ=255)
                                          ccGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCtt                                     >  1:1757209/1‑64 (MQ=255)
                                          ccGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCgg                               >  1:1358318/1‑70 (MQ=255)
                                             cAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGcac                            >  1:5671875/1‑70 (MQ=255)
                                             cAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGcac                            >  1:5262998/1‑70 (MQ=255)
                                              aaGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACaa                          >  1:3155944/1‑71 (MQ=255)
                                               aactCGGCAAAAACATCAAAATCATTACGCGCTTCATAGCGTGGCGGCACCACTTGCTTCATCGGcac                            >  1:4615051/4‑68 (MQ=255)
                                                gATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGc                                                      >  1:1478041/1‑41 (MQ=255)
                                                gATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAata                        >  1:2673821/1‑71 (MQ=255)
                                                 ctCGGCAAAAACATCAAAATCATTACGCGCTTCATAGCGTGGCGGCACCACTTGCTTCATCGGcacc                           >  1:5631644/2‑66 (MQ=255)
                                                  tCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAatat                       >  1:4082233/1‑70 (MQ=255)
                                                    ggCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGcc                                          <  1:1029714/49‑1 (MQ=255)
                                                     gCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTg                   <  1:4203601/71‑1 (MQ=255)
                                                       aaaaACATCAAAATCATTACGCGCTTCATAGCGTGGCGGCACCACTTGCTTCATCGGcacca                          <  1:4822424/62‑3 (MQ=255)
                                                       aaaaACATCAAAATCATTACGCGCTTCATAGCGTGGCGGCACCACTTGCTTCATCGGCACCAGATGCTgat                 <  1:4185684/71‑3 (MQ=255)
                                                       aaaaACATCAAAATCATTACGCGCTTCATAGCGTGGCGGCACCACTTGCTTCATCGGCACCAGATGCTgat                 <  1:3972096/71‑3 (MQ=255)
                                                       aaaaACATCAAAATCATTACGCGCTTCATAGCGTGGCGGCACCACTTGCTTCATCGGCACCAGATGCTgat                 <  1:2588035/71‑3 (MQ=255)
                                                           acatCAAAATCATTACGCGCTTCATAGCGTGGCGGCACCACTTGCTTCATCGGCACCAGATGCt                    <  1:2192890/61‑1 (MQ=255)
                                                           acGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCt             <  1:3570864/71‑1 (MQ=255)
                                                            catCAAAATCATTACGCGCTTCATAGCGTGGCGGCACCACTTGCTTCATCGGCACCAGATGCTGATTACTg            >  1:316453/3‑71 (MQ=255)
                                                             atCAAAATCATTACGCGCTTCATAGCGTGGCGGCACCACTTGCTTCATCGGCACCAGATGCTgat                 <  1:1929920/64‑3 (MQ=255)
                                                             atCAAAATCATTACGCGCTTCATAGCGTGGCGGCACCACTTGCTTCATCGGCACCAGATGCTgat                 <  1:5234501/64‑3 (MQ=255)
                                                              tCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTa          <  1:1852202/71‑1 (MQ=255)
                                                              tCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTa          <  1:579815/71‑1 (MQ=255)
                                                              tCAAAATCATTACGCGCTTCATAGCGTGGCGGCACCACTTGCTTCATCGGCACCAGATGCTGATTACTGTa          <  1:832803/71‑1 (MQ=255)
                                                              tCAAAATCATTACGCGCTTCATAGCGTGGCGGCACCACTTGCTTCATCGGCACCAGATGCTGATTACTGTa          <  1:1980557/71‑1 (MQ=255)
                                                              tCAAAATCATTACGCGCTTCATAGCGTGGCGGCACCACTTGCTTCATCGGCACCAGATGCTGATTACTGTa          <  1:5523638/71‑1 (MQ=255)
                                                              tCAAAATCATTACGCGCTTCATAGCGTGGCGGCACCACTTGCTTCATCGGCACCAGATGCTGATTACTGTa          <  1:2676923/71‑1 (MQ=255)
                                                               cAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTaa         >  1:446779/1‑71 (MQ=255)
                                                                aaaaTCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGc                              >  1:269418/1‑49 (MQ=255)
                                                                aaaaTCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTaa         >  1:1025002/1‑70 (MQ=255)
                                                                aaaaTCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAAt        >  1:283912/1‑71 (MQ=255)
                                                                aaaaTCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAAt        >  1:2360281/1‑71 (MQ=255)
                                                                aaaaTCATTACGCGCTTCATAGCGTGGCGGCACCACTTGCTTCATCGGCACCAGATGCTGAtta               >  1:3493256/1‑63 (MQ=255)
                                                                aaaaTCATTACGCGCTTCATAGCGTGGCGGCACCACTTGCTTCATCGGCACCAGATGCTGAtta               >  1:5047358/1‑63 (MQ=255)
                                                                 aaaTCGTTACGCGCTTCAAATTGCGGAGCAACAGcc                                          <  1:4070980/36‑1 (MQ=255)
                                                                 aaaTCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAAt        >  1:4862634/1‑70 (MQ=255)
                                                                 aaaTCATTACGCGCTTCATAGCGTGGCGGCACCACTTGCTTCATCGGCACCAGATGCTGATTACTGTAATc       <  1:52890/71‑1 (MQ=255)
                                                                  aaTCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCt             >  1:4330394/1‑64 (MQ=255)
                                                                    tCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAata                        >  1:2648123/1‑51 (MQ=255)
                                                                    tCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCt             <  1:5148167/62‑1 (MQ=255)
                                                                    tCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCAcc    >  1:1424967/1‑71 (MQ=255)
                                                                      gTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCgg                               <  1:2361834/42‑1 (MQ=255)
                                                                      gTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCgg                               <  1:2205634/42‑1 (MQ=255)
                                                                      gTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCgg  >  1:1723786/1‑71 (MQ=255)
                                                                      gTTACGCGCTTAAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCgg  >  1:3466036/1‑71 (MQ=255)
                                                                      |                                                                      
TACCTTCGGTATAGATCTCTTTTCCGCCAGGTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGG  >  Exported/1259234‑1259374

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: