Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,259,334 C→T 14.6% Q530Q (CAG→CAA torZ ← trimethylamine N‑oxide reductase system III, catalytic subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,259,3340CT14.6% 283.4 / 31.8 116Q530Q (CAG→CAAtorZtrimethylamine N‑oxide reductase system III, catalytic subunit
Reads supporting (aligned to +/- strand):  ref base C (61/38);  new base T (5/12);  total (66/50)
Fisher's exact test for biased strand distribution p-value = 1.72e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 129 total aligned reads displayed.

GTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATG  >  Exported/1259264‑1259404
                                                                      |                                                                      
gTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGcc                                                                        <  1:861176/71‑1 (MQ=255)
gTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGcc                                                                        <  1:3677550/71‑1 (MQ=255)
       tAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGcc                                                                        >  1:2259436/1‑64 (MQ=255)
       tAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCtt                                                                   <  1:5173178/69‑1 (MQ=255)
         aTTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATc                                                               >  1:946461/1‑71 (MQ=255)
         aTTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATc                                                               >  1:4216608/1‑71 (MQ=255)
          ttCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGcc                                                                        <  1:2233876/61‑1 (MQ=255)
          ttCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGcc                                                                        <  1:4448310/61‑1 (MQ=255)
            ccGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCtt                                                                   >  1:1757209/1‑64 (MQ=255)
            ccGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCgg                                                             >  1:1358318/1‑70 (MQ=255)
            ccGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCgg                                                             >  1:4421697/1‑70 (MQ=255)
               cAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGcac                                                          >  1:5262998/1‑70 (MQ=255)
               cAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGcac                                                          >  1:5671875/1‑70 (MQ=255)
                 aactCGGCAAAAACATCAAAATCATTACGCGCTTCATAGCGTGGCGGCACCACTTGCTTCATCGGcac                                                          >  1:4615051/4‑68 (MQ=255)
                  gATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAata                                                      >  1:2673821/1‑71 (MQ=255)
                   ctCGGCAAAAACATCAAAATCATTACGCGCTTCATAGCGTGGCGGCACCACTTGCTTCATCGGcacc                                                         >  1:5631644/2‑66 (MQ=255)
                    tCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAatat                                                     >  1:4082233/1‑70 (MQ=255)
                      ggCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGcc                                                                        <  1:1029714/49‑1 (MQ=255)
                       gCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTg                                                 <  1:4203601/71‑1 (MQ=255)
                         aaaaACATCAAAATCATTACGCGCTTCATAGCGTGGCGGCACCACTTGCTTCATCGGcacca                                                        <  1:4822424/62‑3 (MQ=255)
                         aaaaACATCAAAATCATTACGCGCTTCATAGCGTGGCGGCACCACTTGCTTCATCGGCACCAGATGCTgat                                               <  1:2588035/71‑3 (MQ=255)
                         aaaaACATCAAAATCATTACGCGCTTCATAGCGTGGCGGCACCACTTGCTTCATCGGCACCAGATGCTgat                                               <  1:3972096/71‑3 (MQ=255)
                         aaaCACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGt                                               <  1:5160103/71‑1 (MQ=255)
                           acacGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACaa                                                        <  1:2597644/60‑1 (MQ=255)
                             acatCAAAATCATTACGCGCTTCATAGCGTGGCGGCACCACTTGCTTCATCGGCACCAGATGCt                                                  <  1:2192890/61‑1 (MQ=255)
                             acGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCt                                           <  1:3570864/71‑1 (MQ=255)
                              catCAAAATCATTACGCGCTTCATAGCGTGGCGGCACCACTTGCTTCATCGGCACCAGATGCTGATTACTg                                          >  1:316453/3‑71 (MQ=255)
                               atCAAAATCATTACGCGCTTCATAGCGTGGCGGCACCACTTGCTTCATCGGCACCAGATGCTgat                                               <  1:1929920/64‑3 (MQ=255)
                                tCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTa                                        <  1:1852202/71‑1 (MQ=255)
                                tCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTa                                        <  1:579815/71‑1 (MQ=255)
                                tCAAAATCATTACGCGCTTCATAGCGTGGCGGCACCACTTGCTTCATCGGCACCAGATGCTGATTACTGTa                                        <  1:1980557/71‑1 (MQ=255)
                                tCAAAATCATTACGCGCTTCATAGCGTGGCGGCACCACTTGCTTCATCGGCACCAGATGCTGATTACTGTa                                        <  1:2676923/71‑1 (MQ=255)
                                tCAAAATCATTACGCGCTTCATAGCGTGGCGGCACCACTTGCTTCATCGGCACCAGATGCTGATTACTGTa                                        <  1:832803/71‑1 (MQ=255)
                                 cAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTaa                                       >  1:446779/1‑71 (MQ=255)
                                  aaaaTCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTaa                                       >  1:1025002/1‑70 (MQ=255)
                                  aaaaTCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAAt                                      >  1:2360281/1‑71 (MQ=255)
                                  aaaaTCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAAt                                      >  1:283912/1‑71 (MQ=255)
                                  aaaaTCATTACGCGCTTCATAGCGTGGCGGCACCACTTGCTTCATCGGCACCAGATGCTGAtta                                             >  1:5047358/1‑63 (MQ=255)
                                   aaaTCGTTACGCGCTTCAAATTGCGGAGCAACAGcc                                                                        <  1:4070980/36‑1 (MQ=255)
                                   aaaTCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAAt                                      >  1:4862634/1‑70 (MQ=255)
                                   aaaTCATTACGCGCTTCATAGCGTGGCGGCACCACTTGCTTCATCGGCACCAGATGCTGATTACTGTAATc                                     <  1:52890/71‑1 (MQ=255)
                                      tCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAata                                                      >  1:2648123/1‑51 (MQ=255)
                                        gTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCgg                                                             <  1:2361834/42‑1 (MQ=255)
                                        gTTACGCGCTTAAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCgg                                >  1:3466036/1‑71 (MQ=255)
                                           aCGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCa                             >  1:836881/1‑71 (MQ=255)
                                            cgcgCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCa                             <  1:498131/70‑1 (MQ=255)
                                             gcgcTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATc                           <  1:5524540/71‑1 (MQ=255)
                                              cgcTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATg                                                    >  1:1939929/1‑45 (MQ=255)
                                               gcTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCAc                                   >  1:5050578/1‑61 (MQ=255)
                                               gcTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGt                         >  1:54923/1‑71 (MQ=255)
                                                cTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGc                                            <  1:3094771/51‑1 (MQ=255)
                                                cTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGc                                            <  1:3651464/51‑1 (MQ=255)
                                                cTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCt                                           >  1:5582834/1‑52 (MQ=255)
                                                 ttCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGt                                         <  1:3300172/53‑1 (MQ=255)
                                                 ttCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGt                                         <  1:937645/53‑1 (MQ=255)
                                                 ttCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTc                        >  1:5549815/1‑70 (MQ=255)
                                                  tCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACaa                                                        >  1:1566426/1‑37 (MQ=255)
                                                  tCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACaa                                                        >  1:3973667/1‑37 (MQ=255)
                                                  tCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTc                        >  1:856251/1‑69 (MQ=255)
                                                   cAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGc                                                   >  1:1447457/1‑41 (MQ=255)
                                                   cAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCa                       <  1:3461462/69‑1 (MQ=255)
                                                    aaaTTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATc                           >  1:1534788/1‑64 (MQ=255)
                                                    aaaTTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGt                    <  1:3778107/71‑1 (MQ=255)
                                                     aaTTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTc                   <  1:2069629/71‑1 (MQ=255)
                                                      aTTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCt                                                  <  1:4155405/39‑1 (MQ=255)
                                                      aTTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCt                                                  <  1:3466968/39‑1 (MQ=255)
                                                      aTTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCt                                                  <  1:3025261/39‑1 (MQ=255)
                                                      aTTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCt                                           <  1:4320656/46‑1 (MQ=255)
                                                      aTTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTc                   <  1:5453119/70‑1 (MQ=255)
                                                       ttGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAAtac                                    >  1:1033626/1‑50 (MQ=255)
                                                       ttGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCAt                 <  1:5566292/71‑1 (MQ=255)
                                                        tGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCAtt                >  1:2781082/1‑71 (MQ=255)
                                                         gCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCAtt                <  1:5179392/70‑1 (MQ=255)
                                                          cGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGt                                               <  1:2151936/38‑1 (MQ=255)
                                                          cGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTgc              >  1:250120/1‑71 (MQ=255)
                                                            gAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCa                  <  1:2915445/65‑1 (MQ=255)
                                                             aGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCt           >  1:4602458/1‑71 (MQ=255)
                                                               cAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTc          >  1:1774422/1‑70 (MQ=255)
                                                                aaCAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCa         >  1:1230307/1‑70 (MQ=255)
                                                                 aCAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCTTCAAGTCATTGCGCTCaaa       >  1:5316869/1‑71 (MQ=255)
                                                                 aCAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCAtt                >  1:4889175/1‑62 (MQ=255)
                                                                 aCAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCAtt                >  1:5518979/1‑62 (MQ=255)
                                                                 aCAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCaaa       <  1:4148397/71‑1 (MQ=255)
                                                                 aCAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCaaa       >  1:3573577/1‑71 (MQ=255)
                                                                 aCAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCaaa       >  1:4710690/1‑71 (MQ=255)
                                                                 aCAGCCTGCTTCATAGGCACAATATGCTGGTTGATGt                                         >  1:5093352/1‑37 (MQ=38)
                                                                  cAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGt                    <  1:195285/57‑1 (MQ=255)
                                                                  cAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGt                    <  1:878753/57‑1 (MQ=255)
                                                                  cAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAAc      >  1:5322952/1‑71 (MQ=255)
                                                                  cAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAAc      <  1:658400/71‑1 (MQ=255)
                                                                   aGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAAc      >  1:1588531/1‑70 (MQ=255)
                                                                   aGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAAc      >  1:4465805/1‑70 (MQ=255)
                                                                    gCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCa                  >  1:3182833/1‑57 (MQ=255)
                                                                    gCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCa                  >  1:1258353/1‑57 (MQ=255)
                                                                    gCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCa                  >  1:1132808/1‑57 (MQ=255)
                                                                    gCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCa                  >  1:909550/1‑57 (MQ=255)
                                                                    gCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGa    >  1:2465211/1‑71 (MQ=255)
                                                                     ccTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCaa        <  1:2712168/66‑1 (MQ=255)
                                                                     ccTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCaa        <  1:320250/66‑1 (MQ=255)
                                                                      cTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGAtg  <  1:71537/71‑1 (MQ=255)
                                                                      |                                                                      
GTTTGAGTAATTCCGCAAGATCGGCAAACACGTCAAAATCGTTACGCGCTTCAAATTGCGGAGCAACAGCCTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATG  >  Exported/1259264‑1259404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: