Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,259,403 T→G 5.5% T507T (ACA→ACC torZ ← trimethylamine N‑oxide reductase system III, catalytic subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,259,4030TG 5.5% 341.1 / 11.3 128T507T (ACA→ACCtorZtrimethylamine N‑oxide reductase system III, catalytic subunit
Reads supporting (aligned to +/- strand):  ref base T (43/78);  new base G (3/4);  total (46/82)
Fisher's exact test for biased strand distribution p-value = 7.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01

Only 100 of 153 total aligned reads displayed.

CTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAGTAGCATTCAGAAACGACGA  >  Exported/1259334‑1259470
                                                                     |                                                                   
cTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGAtg                                                                    <  1:2590335/71‑1 (MQ=255)
 tGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGAtgt                                                                   >  1:2879637/1‑71 (MQ=255)
 tGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGAtgt                                                                   >  1:1590814/1‑71 (MQ=255)
 tGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCCCTCAAACGAtgt                                                                   >  1:3636816/1‑71 (MQ=255)
  gCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGAtgtg                                                                  >  1:3430096/1‑71 (MQ=255)
  gCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGAtgtg                                                                  >  1:5045382/1‑71 (MQ=255)
  gCTTCATCGGCACAATATGCTGGTTGCTGAAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGAtgtg                                                                  >  1:3960892/1‑71 (MQ=255)
   cTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGAtgtg                                                                  <  1:2951810/70‑1 (MQ=255)
     tCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATgtggt                                                                >  1:3032629/1‑70 (MQ=255)
     tCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATgtggt                                                                >  1:4743670/1‑70 (MQ=255)
      cATCGGCACCAGATGCTGATTACTGTAATCACCGGTCATGGTGAGATCATTACGCTCAAAAGAGgtagtc                                                               <  1:1185271/70‑2 (MQ=255)
       aTCGGCACAATATGCTGGTTGCTGTAATCACCCGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGAt                                                             <  1:5504730/71‑1 (MQ=255)
        tCGGCACCAGATGCTGATTACTGTAATCACCGGTCATGGTGAGATCATTACGCTCAAAAGAGgtagt                                                                <  1:221067/67‑1 (MQ=255)
        tCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGAtgt                                                                   <  1:2926890/64‑1 (MQ=255)
         cGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCg                                                           >  1:2755171/1‑71 (MQ=255)
          ggCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCgg                                                          >  1:3561097/1‑71 (MQ=255)
          ggCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCgg                                                          >  1:523185/1‑71 (MQ=255)
          ggCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCgg                                                          >  1:4789844/1‑71 (MQ=255)
             acaATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTaa                                                       <  1:4583160/71‑1 (MQ=255)
              caATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTaa                                                       <  1:490495/70‑1 (MQ=255)
                atatGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATAc                                                    <  1:3534282/71‑1 (MQ=255)
                  atGCTGATTACTGTAATCACCGGTCATGGTGAGATCATTACGCTCAAAAGAggtag                                                                 <  1:2080798/56‑3 (MQ=255)
                  atGCTGATTACTGTAATCACCGGTCATGGTGAGATCATTACGCTCAAAAGAggtag                                                                 <  1:2164032/56‑3 (MQ=255)
                   tGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCg                                                           <  1:1314701/61‑1 (MQ=255)
                    gCTGATTACTGTAATCACCGGTCATGGTGAGATCATTACGCTCAAAAGAGgtagtc                                                               >  1:4088047/1‑55 (MQ=255)
                      tGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATc                                              >  1:3721769/1‑71 (MQ=255)
                       ggTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGAtgtg                                                                  <  1:5346000/50‑1 (MQ=255)
                       ggTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGAtgtg                                                                  <  1:4555315/50‑1 (MQ=255)
                       ggTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATgtgg                                                                 >  1:1739802/1‑51 (MQ=255)
                         ttGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTaa                                                       <  1:2762776/59‑1 (MQ=255)
                         ttGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGc                                           <  1:686288/71‑1 (MQ=255)
                         ttGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGc                                           >  1:1784590/1‑71 (MQ=255)
                          tGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCg                                          >  1:4543770/1‑71 (MQ=255)
                            cTGTAATCACCGGTCATCGTCAGGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGatat                                               <  1:1289311/64‑1 (MQ=255)
                            cTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGatat                                               <  1:1794790/64‑1 (MQ=255)
                            cTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGatat                                               <  1:5223313/64‑1 (MQ=255)
                            cTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCgtg                                        <  1:3827125/71‑1 (MQ=255)
                            cTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCgtg                                        <  1:444180/71‑1 (MQ=255)
                             tGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGat                                                 <  1:4050599/61‑1 (MQ=255)
                               tAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAAt                                                      <  1:3663348/54‑1 (MQ=255)
                               tAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAAt                                                      <  1:862157/54‑1 (MQ=255)
                               tAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCgtgt                                       <  1:3881979/69‑1 (MQ=255)
                               tAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCgtgt                                       <  1:2722238/69‑1 (MQ=255)
                                aaTCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGatat                                               >  1:1681590/1‑60 (MQ=255)
                                aaTCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTg                                    >  1:1209252/1‑71 (MQ=255)
                                 aTCACCGGTCATGGTGAGATCATTACGCTCAAAAGAGGTAGTCGCAGGCAGAACGATATCCGCGTGtttt                                    <  1:2177162/70‑2 (MQ=255)
                                 aTCACCGGTCATGGTGAGATCATTACGCTCAAAAGAGGTAGTCGCAGGCAGAACGATATCCGCGTGttt                                     <  1:5589473/69‑1 (MQ=255)
                                  tCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGgc                                  <  1:4492868/71‑1 (MQ=255)
                                   cACCGGTCATCGTCAAGTCATTGCGCTCAAACGAtgtg                                                                  >  1:2396140/1‑38 (MQ=255)
                                   cACCGGTCATCGTCAAGTCATTGCGCTCAAACGAtgtg                                                                  >  1:5627515/1‑38 (MQ=255)
                                      cGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCgtg                                        <  1:4944730/61‑1 (MQ=255)
                                       ggTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATa                                                     >  1:2897523/1‑47 (MQ=255)
                                        gTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACg                                                   <  1:4284283/48‑1 (MQ=255)
                                        gTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACg                                                   <  1:3171506/48‑1 (MQ=255)
                                        gTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCtgctgc                            >  1:5463484/1‑71 (MQ=255)
                                          cATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCg                                                           >  1:764864/1‑38 (MQ=255)
                                           aTCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTa                                                        >  1:4650951/1‑40 (MQ=255)
                                           aTCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTa                                                        >  1:4120518/1‑40 (MQ=255)
                                           aTCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCgg                          <  1:765810/70‑1 (MQ=255)
                                           aTCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCAGCGTGTTTGGCTGCTGCgg                          <  1:4417518/70‑1 (MQ=255)
                                            tCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTc                        <  1:2384150/71‑1 (MQ=255)
                                            tCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTc                        <  1:2339613/71‑1 (MQ=255)
                                            tCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTc                        <  1:5424269/71‑1 (MQ=255)
                                            tCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTc                        <  1:3350016/71‑1 (MQ=255)
                                             cGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTc                        >  1:1160132/1‑70 (MQ=255)
                                              gTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGtt                                      >  1:2228763/1‑55 (MQ=255)
                                              gTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCg                           >  1:5668380/1‑66 (MQ=255)
                                               tCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTc                        <  1:420713/68‑1 (MQ=255)
                                               tCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTc                        <  1:2079134/68‑1 (MQ=255)
                                               tCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTc                        <  1:5381198/68‑1 (MQ=255)
                                               tCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTc                        <  1:5374527/68‑1 (MQ=255)
                                                 aaGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGgctgc                               <  1:1397707/59‑1 (MQ=255)
                                                 aaGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGgctgc                               <  1:2487971/59‑1 (MQ=255)
                                                 aaGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGgctgc                               <  1:4288156/59‑1 (MQ=255)
                                                 aaGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGgctgc                               <  1:3013925/59‑1 (MQ=255)
                                                 aaGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAg                     >  1:5511488/1‑69 (MQ=255)
                                                 aaGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAg                     >  1:1000552/1‑69 (MQ=255)
                                                   gTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAGTAGc                 <  1:3364543/71‑1 (MQ=255)
                                                    tCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCgtg                                        <  1:1042813/47‑1 (MQ=255)
                                                    tCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCgtg                                        <  1:668306/47‑1 (MQ=255)
                                                    tCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCgtg                                        <  1:4574583/47‑1 (MQ=255)
                                                    tCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAg                     >  1:169152/1‑66 (MQ=255)
                                                      aTTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGttt                                     <  1:1238108/48‑1 (MQ=255)
                                                      aTTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGttt                                     <  1:4913971/48‑1 (MQ=255)
                                                      aTTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAGTAGCAtt              <  1:2605661/71‑1 (MQ=255)
                                                       ttGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGtt                                      <  1:3445016/46‑1 (MQ=255)
                                                        tGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTgg                                   >  1:1819848/1‑48 (MQ=255)
                                                         gcgcTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAg                     >  1:3466798/1‑61 (MQ=255)
                                                            cTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTc                        >  1:1773532/1‑55 (MQ=255)
                                                            cTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTc                        >  1:1655943/1‑55 (MQ=255)
                                                            cTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTc                        >  1:3491838/1‑55 (MQ=255)
                                                             tCAAACGATGTGGTGATCGGTAATACGATATCTGCgtg                                        <  1:4693206/38‑1 (MQ=255)
                                                             tCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTc                        <  1:3965586/54‑1 (MQ=255)
                                                             tCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTc                        <  1:5574362/54‑1 (MQ=255)
                                                              cAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAGTAGCATTCAGAAac       >  1:5111383/1‑70 (MQ=255)
                                                               aaaCGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAGTAGCGTTCAGAAac       <  1:4940201/69‑1 (MQ=255)
                                                               aaaCGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAGTAGCATTCAGAAacga     >  1:1823471/1‑71 (MQ=255)
                                                               aaaCGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAGTAGCATTCAGAAacga     >  1:2865340/1‑71 (MQ=255)
                                                               aaaCGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAGTAGCATTCAGAAac       <  1:1455361/69‑1 (MQ=255)
                                                               aaaCGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAGTAGCATTCAGAAac       <  1:1627499/69‑1 (MQ=255)
                                                                     |                                                                   
CTGCTTCATCGGCACAATATGCTGGTTGCTGTAATCACCGGTCATCGTCAAGTCATTGCGCTCAAACGATGTGGTGATCGGTAATACGATATCTGCGTGTTTGGCTGCTGCGGTCCAGTAGCATTCAGAAACGACGA  >  Exported/1259334‑1259470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: