| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,259,850 | C→T | 16.5% | Q358Q (CAG→CAA) | torZ ← | trimethylamine N‑oxide reductase system III, catalytic subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,259,850 | 0 | C | T | 16.5% | 212.1 / 29.6 | 91 | Q358Q (CAG→CAA) | torZ | trimethylamine N‑oxide reductase system III, catalytic subunit |
| Reads supporting (aligned to +/- strand): ref base C (44/32); new base T (8/7); total (52/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.81e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01 | |||||||||||
CGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCGATTCGCCGCCATCAGTTCAGCGAGTTTGAC > Exported/1259781‑1259918 | cGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGGATCCAGTGTTTCTGTTCACCATATTGCt < 1:3908737/71‑1 (MQ=255) tgtACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGgc > 1:2667483/1‑71 (MQ=255) tttGCCCCAACATTGCTGCCAGCGTGACGATCATCCAGTGTTTTTGCTCACCAAACTGTTGGCGCTGCAt > 1:1989388/3‑70 (MQ=255) tttGCCCCAACATTGCTGCCAGCGTGACGATCATCCAGTGTTTTTGCTCACCAAACTGTTGGCGCTGCAt > 1:4054543/3‑70 (MQ=255) tCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCt < 1:148507/60‑1 (MQ=255) cTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTc > 1:4127653/1‑71 (MQ=255) cTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTc > 1:3686733/1‑71 (MQ=255) cTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTc > 1:761480/1‑71 (MQ=255) tGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTcc > 1:5063077/1‑71 (MQ=255) cccTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTcccc < 1:2038243/71‑1 (MQ=255) cccTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTcccc < 1:4124712/71‑1 (MQ=255) cccaaCATTGCTGCCAGCGTGACGATCATCCAGTGTTTTTGCTCACCAAACTGTTGGCGCTGCATTccc > 1:175182/4‑69 (MQ=255) cccaaCATTGCTGCCAGCGTGACGATCATCCAGTGTTTTTGCTCACCAAACTGTTGGCGCTGCATTccc > 1:5694394/4‑69 (MQ=255) cccaaCATTGCTGCCAGCGTGACGATCATCCAGTGTTTTTGCTCACCAAACTGTTGGCGCTGCATTccc > 1:1510680/4‑69 (MQ=255) cccaaCATTGCTGCCAGCGTGACGATCATCCAGTGTTTTTGCTCAACAAACTGTTGGCGCTGCATTCCCCa < 1:5584119/68‑1 (MQ=255) ccTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTcccc < 1:3988489/70‑1 (MQ=255) tAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCt < 1:1665276/59‑1 (MQ=255) tAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTccc > 1:3949943/1‑67 (MQ=255) tAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGc > 1:3334757/1‑71 (MQ=255) aaCATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGcc > 1:1835596/1‑71 (MQ=255) aCATTGCTGCCAGCGTGACGATCATCCAGTGTTTTTGCTCACCAAACTGTTgg > 1:205362/1‑53 (MQ=255) aCATTGCTGCCAGCGTGACGATCATCCAGTGTTTTTGCTCACCAAACTGTTgg > 1:4864383/1‑53 (MQ=255) cATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTg > 1:2768747/1‑71 (MQ=255) aTCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGc < 1:5090975/71‑1 (MQ=255) gctgccAGCGTGACGATCATCCAGTGTTTTTGCTCACCAAACTGTTGGCGCTGCATTCCCCAGCCTGCCAt > 1:2796622/4‑71 (MQ=255) gccgccAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCa > 1:3956571/1‑62 (MQ=255) ctgccAGCGTGACGATCATCCAGTGTTTTTGCTCACCAAACTGTTGGCGCTGCATTCCCCAGCCTGCCATc < 1:1108008/69‑1 (MQ=255) ccgccAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATc < 1:4935187/71‑1 (MQ=255) tgccAGCGTGACGATCATCCAGTGTTTTTGCTCACCAAACTGTTGGCGCTGCATTc < 1:4673168/55‑1 (MQ=255) gccAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGc > 1:4741654/1‑65 (MQ=255) gccAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGc > 1:2199633/1‑65 (MQ=255) gccAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAg < 1:2306961/71‑1 (MQ=255) gccAGCGTCACCAGCATCCAGTGTTTCTGTTAACCATATTGCTGGCGCTGAATTCCCCAGCCTGc > 1:100003/1‑65 (MQ=255) aGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCAt > 1:557917/1‑65 (MQ=255) aGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCAt > 1:3026975/1‑65 (MQ=255) gCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAg < 1:4246466/67‑1 (MQ=255) cGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATtg < 1:3602227/71‑1 (MQ=255) cACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATtgtg > 1:88160/1‑70 (MQ=255) aCCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGc > 1:5606113/1‑70 (MQ=255) ccAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCGa < 1:1211039/71‑1 (MQ=255) cAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCGAt > 1:4539136/1‑71 (MQ=255) cAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCGAt > 1:97902/1‑71 (MQ=255) cAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCGAt > 1:2999096/1‑71 (MQ=255) aGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCGAtt < 1:5489981/71‑1 (MQ=255) aTCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCGATTcg < 1:5386591/70‑1 (MQ=255) tCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCGAt < 1:5036926/66‑1 (MQ=255) cAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCGATTcgccg < 1:3492910/70‑1 (MQ=255) aGTGTTTCTGTTCACCATATTGCTGCCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCGATTcgccgc > 1:2695104/1‑70 (MQ=255) gtgtTTCTGTTCACCATATTGCTGGCGCTGAATTcc < 1:4637152/36‑1 (MQ=255) gtgtTTCTGTTCACCATATTGCTGGCGCTGAATTcc < 1:510371/36‑1 (MQ=255) gtgtTTCTGTTCACCATATTGCTGGCGCTGAATTcc < 1:1023609/36‑1 (MQ=255) gtTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCa > 1:4169970/1‑51 (MQ=255) ttttTGCTCACCAAACTGTTGGGGCTGCATTCCCCAGCCTGCCATCa < 1:3389962/47‑1 (MQ=16) ttttTGCTCACCAAACTGTTGGCGCTGCATTCCCCAGCCTGCCATCa < 1:2860386/47‑1 (MQ=19) ttttTGCTCACCAAACTGTTGGCGCTGCATTCCCCAGCCTGCCATCa < 1:5131128/47‑1 (MQ=19) ttttTGCTCACCAAACTGTTGGCGCTGCATTCCCCAGCCTGCCATCa < 1:3626513/47‑1 (MQ=19) tttCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCGATTCGCCGCCAt > 1:3645741/1‑69 (MQ=255) ttCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCAt > 1:4300713/1‑44 (MQ=255) tCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCa > 1:5561233/1‑42 (MQ=255) tCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCa > 1:3307494/1‑42 (MQ=255) tCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCa > 1:3588402/1‑42 (MQ=255) tCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCa > 1:407781/1‑42 (MQ=255) tCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATtg > 1:937116/1‑51 (MQ=255) tCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCGATTCGCCGCCATCAGt > 1:1023915/1‑71 (MQ=255) cTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCGATTCGCCGCCATCAGt < 1:325743/70‑1 (MQ=255) cTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCGATTCGCCGCCATCAGt < 1:4283614/70‑1 (MQ=255) tGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCGAtt > 1:5607194/1‑56 (MQ=255) tGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCGATTCGCCGCCATCAGtt > 1:3734576/1‑70 (MQ=255) ttCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGc < 1:4983520/50‑1 (MQ=255) ttCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCGATTCGCCGCCATCAGTTCAg > 1:5009449/1‑71 (MQ=255) cACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCGa > 1:4488154/1‑50 (MQ=255) cACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCGATTCGCCGCCATCAGTTCAGc > 1:4959383/1‑70 (MQ=255) aCCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGc < 1:4316482/47‑1 (MQ=255) aCCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCGATTCGCCGCCATCAGTTCAGCGa < 1:715806/71‑1 (MQ=255) aCCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCGATTCGCCGCCATCAGTTCAGCGa < 1:5435687/71‑1 (MQ=255) cATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCGATTCGCCGCCATCAGTTCAGCGAGt < 1:4070365/71‑1 (MQ=255) atatTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCGATTCGCCGCCATc < 1:3064520/58‑1 (MQ=255) atatTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCGATTCGCCGCCATCAGTTCAGCGAGtt < 1:3701137/71‑1 (MQ=255) tatTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCGATTCGCCGCCATCAGTTCAGCGAGttt < 1:2363911/71‑1 (MQ=255) tatTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCGATTCGCCGCCATCAGTTCAGCGAGttt < 1:2157096/71‑1 (MQ=255) tatTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCGATTCGCCGCCATCAGTTCAGCGAGttt < 1:706586/71‑1 (MQ=255) tatTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCGATTCGCCGCCATCAGTTCAGCGAGttt > 1:1869497/1‑71 (MQ=255) tatTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCGATTCGCCGCCATCAGTTCAGCGAGttt < 1:3416060/71‑1 (MQ=255) atTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCGATTCGCCGCCATCAGTTCAGCGAGttt > 1:2592434/1‑70 (MQ=255) ttGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCGa > 1:5399976/1‑43 (MQ=255) ttGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCGa > 1:3128781/1‑43 (MQ=255) ttGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCGATTCGCCGCCATCAGTTCAGCGAGTTTGa > 1:3276143/1‑71 (MQ=255) ttGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCGATTCGCCGCCATCAGTTCAGCGAGTTTGa > 1:4515181/1‑71 (MQ=255) ttGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCGATTCGCCGCCATCAGTTCAGCGAGTTTGa > 1:4137337/1‑71 (MQ=255) gCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCGATTCGCCGCCATCAGTTCAGCGAGTTTGAc > 1:4779950/1‑70 (MQ=255) gCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCGATTCGCCGCCATCAGTTCAGCGAGTTTGAc > 1:4731542/1‑70 (MQ=255) | CGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATTGTGCGATTCGCCGCCATCAGTTCAGCGAGTTTGAC > Exported/1259781‑1259918 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |