| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 223,023 | C→T | 8.3% | intergenic (+78/‑102) | lpcA → / → prfH | D‑sedoheptulose 7‑phosphate isomerase/predicted peptide chain release factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 223,023 | 0 | C | T | 8.3% | 340.4 / 16.3 | 132 | intergenic (+78/‑102) | lpcA/prfH | D‑sedoheptulose 7‑phosphate isomerase/predicted peptide chain release factor |
| Reads supporting (aligned to +/- strand): ref base C (55/66); new base T (6/5); total (61/71) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.54e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.63e-01 | |||||||||||
Only 100 of 136 total aligned reads displayed.
CGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGTTCACGCCGCATCCGACCTGAA > Exported/222952‑223092 | tgTAGGCCGGACAAGACGTTTA‑‑TCGCATCCGGCAGTCGTGTGCAGATGCCTGATGCGACGCTAACGCGTCt < 1:3991590/70‑1 (MQ=9)tgTAGGCCGGACAAGACGTTTA‑‑TCGCATCCGGCAGTCGTGTGCAGATGCCTGATGCGACGCTAACGCGTCt < 1:4325091/70‑1 (MQ=9) ggCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTa < 1:3968490/71‑1 (MQ=255) ggCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTa > 1:4039942/1‑71 (MQ=255) ggCCGGACAAGACGTTTA‑‑TCGCATCCGGCAGTCGTGTGCAGATGCCTGATGCGACGCTAACGCGTCTTAt < 1:4582333/70‑1 (MQ=9) ggCCGGACAAGACGTTTA‑‑TCGCATCCGGCAGTCGTGTGCAGATGCCTGATGCGACGCTAACGCGTCTTAt < 1:4683535/70‑1 (MQ=9) ggATAAGGCGTTTACGCCGCATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGCTAACGCGTCTTAt > 1:1063915/1‑68 (MQ=14) ggACAAGACGTTTA‑‑TCGCATCCGGCAGTCGTGTGCAGATGCCTGATGCGACGCTAACGCGTCTTATc < 1:3573976/67‑1 (MQ=9) gATAAGGCGTTTACGCCGCATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGCTAACGCGTCTTATCagg > 1:824945/1‑69 (MQ=11) gATAAGGCGTTTACGCCGCATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGCTAACGCGTCTTATCagg > 1:4350560/1‑69 (MQ=11) tAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGcc < 1:3814930/71‑1 (MQ=11) aggcGTTTATGCCGCATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTa < 1:1875605/68‑1 (MQ=11) aggcGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCt < 1:75157/67‑1 (MQ=11) gTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGc > 1:2237443/1‑64 (MQ=255) gTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACaaa > 1:2742367/1‑71 (MQ=255) tttATGCCGCATCCGGCAGTTATGCGCAGATGCCTGATGCGACGCTGGCGCGTCTTATc < 1:3095830/59‑1 (MQ=11) ttACGCCGCATCCGGCATTTGTGCGATGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATc > 1:2157744/1‑71 (MQ=255) tACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCt > 1:5551855/1‑71 (MQ=255) caCGCCGCATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGCTAACGCGTCTTATCAGACCTACAAAttc > 1:4035262/2‑69 (MQ=11) caCGCCGCATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGCTAACGCGTCTTATCAGACCTACAAAttc > 1:2380082/2‑69 (MQ=11) cgccgcATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGCTAACGCGTCTTATCAGGCCTACAAAttct < 1:3513868/70‑4 (MQ=11) ccgcATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTAc < 1:5228399/71‑1 (MQ=255) ccgcATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTAc > 1:4984833/1‑71 (MQ=255) gcATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGCTAACGCGTCTTATCAGGCCTACa < 1:1360600/60‑1 (MQ=11) cATCCGGCA‑CTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTAcg < 1:1850183/58‑2 (MQ=14) tCCGGCAGTCGTGCGCAGATGCCTGATGCGACGCTAACGCGTCTTATCAGGCCTACAAAttc > 1:1944324/1‑60 (MQ=11) cATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCg > 1:374981/1‑69 (MQ=255) cATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTa < 1:1381827/71‑1 (MQ=255) tttGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACaa < 1:1505484/51‑1 (MQ=255) ctgtgtgCCGATGCCTGATGCGACGCTGACGCGTTTTATCAt < 1:1633189/41‑1 (MQ=14) ctgtgtgCCGATGCCTGATGCGACGCTGACGCGTTTTATCAt < 1:3307470/41‑1 (MQ=14) tgtgtgCCGATGCCTGATGCGACGCTGACGCGTTTTAt < 1:4718543/38‑1 (MQ=14) tgtgtgCCGATGCCTGATGCGACGCTGACGCGTTTTAt < 1:599282/38‑1 (MQ=14) tgtgCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAg > 1:3118635/1‑68 (MQ=255) tgtgCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAg > 1:2547384/1‑68 (MQ=255) tgtgCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGcc > 1:2958194/1‑71 (MQ=255) gtgtGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGc > 1:1661702/1‑42 (MQ=14) gtgtGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGc > 1:1834998/1‑42 (MQ=14) gtgtGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTa > 1:3616852/1‑45 (MQ=11) gtgtGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTa > 1:3473134/1‑45 (MQ=11) gtgCGCAGATGCCTGATGCGACGCTAACGCGTCTTATCAGGCCTACAAAtt > 1:4971846/1‑50 (MQ=14) tgCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGa < 1:2791128/71‑1 (MQ=255) cgcTGATGCCTGATGTGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGaa < 1:2414668/70‑1 (MQ=255) cgcTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGcc < 1:1277350/67‑1 (MQ=255) ctgatgcctgatgcGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGaata < 1:4647595/70‑1 (MQ=255) cctatgcctgatgcgacGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgtt < 1:1427658‑M1/58‑20 (MQ=255) tgatgcctgatgcGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGaataat > 1:3498067/1‑71 (MQ=255) ctatgcctgatgcgaCGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatc < 1:1437379‑M1/69‑31 (MQ=255) ctatgcctgatgcgaCGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatc < 1:4228679‑M1/69‑31 (MQ=255) ctatgcctgatgcgaCGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgttta > 1:314186‑M1/3‑41 (MQ=255) ctatgcctgatgcgaCGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgttta > 1:875263‑M1/3‑41 (MQ=255) tatgcctgatgcgACGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatcc > 1:2101907‑M1/2‑40 (MQ=255) tatgcctgatgcgACGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatcc > 1:1903227‑M1/2‑40 (MQ=255) tatgcctgatgcgACGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatcc > 1:1004805‑M1/2‑40 (MQ=255) gatgcctgatgcGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATg > 1:1984470/1‑71 (MQ=255) atgcctgatgcGACGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatc > 1:2946535‑M1/1‑39 (MQ=255) gcctgatgcGACGCTGACGCGTCTTATCATGCCTACAAATCTCTACGCGAACCGTAGGc > 1:4099538/1‑59 (MQ=255) gcctgatgcGACGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtat < 1:2568832‑M1/53‑17 (MQ=255) cctgatgcGACGCTGACGCGTCTTATCATGCCTACAAAt < 1:4187097/39‑1 (MQ=255) ctgatgcGACGCTGACGCGTCTTATCATGCCTACAAATCTGTAcgcg > 1:88171/1‑47 (MQ=255) ctgatgcGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTa < 1:1887819/54‑1 (MQ=255) tgatgcGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGtt < 1:2198426/69‑1 (MQ=255) tgatgcGACGCTAACGCGTCTTATCATGCCTACAAATcgc < 1:1517671/40‑3 (MQ=14) tgatgcGACGCTAACGCGTCTTATCATGCCTACAAATcgc < 1:5430110/40‑3 (MQ=14) gatgcGACGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgtt < 1:5660260‑M1/52‑20 (MQ=255) gatgcGACGCTGAAGCGTCTTATCAAGCCTACAtagcactgccacgtatgtttacaccgcatccggcata > 1:818729‑M1/1‑33 (MQ=255) atgcGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGTTCa > 1:1062898/1‑69 (MQ=255) atgcGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGTTCAcg > 1:3836249/1‑71 (MQ=255) atgcGACGCTGAAGCGTCTTATCATGCCTACAtagcac > 1:4033205‑M1/1‑32 (MQ=255) tgcGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCg < 1:824115/48‑1 (MQ=255) tgcGACGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatccggcataa < 1:2484138‑M1/69‑39 (MQ=255) tgcGACGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatccggcataa < 1:4048171‑M1/69‑39 (MQ=255) cGACGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatccggcataaaaac < 1:274881‑M1/71‑43 (MQ=255) gACGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatccggcataaaaaca > 1:1857692‑M1/1‑28 (MQ=255) aCGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatccggcataaaaacac < 1:2096420‑M1/71‑45 (MQ=255) aCGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatccggcataaaaa < 1:3098024‑M1/68‑42 (MQ=255) aCGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatccggcataaaaa < 1:1281470‑M1/68‑42 (MQ=255) aCGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatg < 1:769342‑M1/44‑18 (MQ=255) aCGCTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatg < 1:1429198‑M1/44‑18 (MQ=255) cGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGTTCACGCCGCAt > 1:4647210/1‑71 (MQ=255) gCTGACGCGTCTTATCATGCCTACAAATCTGTAcgcg > 1:4238763/1‑37 (MQ=255) gCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGTTCACGCCGCATc > 1:3843026/1‑71 (MQ=255) cTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatccggcataaaaacacgcg < 1:4858968‑M1/71‑48 (MQ=255) cTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatccggcataaaaacacgcg < 1:3770558‑M1/71‑48 (MQ=255) cTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatccggcataaa < 1:1279892‑M1/63‑40 (MQ=255) cTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatccggcataaa < 1:2391419‑M1/63‑40 (MQ=255) cTGAAGCGTCTTATCATGCCTACAtagcactgccacgtatgttta < 1:5457551‑M1/45‑22 (MQ=255) tGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGcc < 1:1671084/46‑1 (MQ=255) gACGCGACTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGTTCACGCCGCATCCGa > 1:732620/1‑71 (MQ=255) agcgtCTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatccggcataaaaacacgcgcac < 1:3150977‑M1/69‑51 (MQ=255) cgTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGtt < 1:3572125/53‑1 (MQ=255) cgTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGtt < 1:3094885/53‑1 (MQ=255) cgTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGtt < 1:3274196/53‑1 (MQ=255) tCTTATCATGCCTACAtagcactgccacgtatgttttcaccgcatccggcatat < 1:208239‑M1/54‑39 (MQ=255) tCTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatccggcataaa < 1:5660959‑M1/55‑40 (MQ=255) tCTTATCATGCCTACAtagcactgccacgtatgtttacaccgcatccggcataaa < 1:1078565‑M1/55‑40 (MQ=255) tCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGTTCa > 1:399649/1‑53 (MQ=255) tCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGTTCACGCCGCATCCGACCTGaa < 1:4722159/71‑1 (MQ=255) tCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGTTCACGCCGCATCCGACCTGaa < 1:4840597/71‑1 (MQ=255) cTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGa < 1:5284462/40‑1 (MQ=255) | CGTAGGCCGGATAAGACGTTTACGCCGCATCCGGCATTTGTGCGCTGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTACAAATCTGTACGCGAACCGTAGGCCGAATAATGCGTTCACGCCGCATCCGACCTGAA > Exported/222952‑223092 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |