Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	1,279,226	C→G	19.1%	V335V (GTC→GTG) ‡	yeeJ →	adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	1,279,226	0	C	G	19.1%	196.1 / 37.0	89	V335V (GTC→GTG) ‡	yeeJ	adhesin
Reads supporting (aligned to +/- strand):  ref base C (35/37);  new base G (7/10);  total (42/47)
Fisher's exact test for biased strand distribution p-value = 6.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

CAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGC  >  Exported/1279160‑1279294                                                                   |                                                                     cAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATg                                                                  <  1:3226294/71‑1 (MQ=255) cAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATg                                                                  <  1:2388528/71‑1 (MQ=255)  aaTGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATg                                                                  >  1:5648913/1‑70 (MQ=255)  aaTGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATg                                                                  >  1:5592350/1‑70 (MQ=255)  aaTGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATg                                                                  >  1:5107887/1‑70 (MQ=255)  aaTGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATg                                                                  >  1:4022573/1‑70 (MQ=255)  aaTGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATg                                                                  >  1:2544932/1‑70 (MQ=255)  aaTGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATg                                                                  >  1:1558620/1‑70 (MQ=255)   aTGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGaa                                                                <  1:1123278/71‑1 (MQ=255)   aTGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGaa                                                                <  1:1320156/71‑1 (MQ=255)    tGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGaa                                                                >  1:653716/1‑70 (MQ=255)    tGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAAc                                                               <  1:3253910/71‑1 (MQ=255)     ggCTGGGATTTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGaa                                                                >  1:670710/1‑69 (MQ=255)        tGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGta                                                           >  1:1678832/1‑71 (MQ=255)          ggATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGtat                                                          >  1:4901693/1‑70 (MQ=255)            aTGTACACGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGtatta                                                        >  1:5159/1‑70 (MQ=255)             tGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCt                                                                     <  1:4937695/56‑1 (MQ=255)             tGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCt                                                                     <  1:5445219/56‑1 (MQ=255)             tGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATg                                                      <  1:3605568/71‑1 (MQ=255)             tGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATg                                                      >  1:1143794/1‑71 (MQ=255)               tACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGa                                                                 <  1:3076240/58‑1 (MQ=255)               tACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGa                                                                 <  1:749481/58‑1 (MQ=255)               tACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGc                                                    <  1:3654211/71‑1 (MQ=255)                 cgcgCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGa                                                  >  1:4276887/1‑71 (MQ=255)                   cgcAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATg                                                <  1:3825451/71‑1 (MQ=255)                   cgcAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATg                                                <  1:173517/71‑1 (MQ=255)                   cgcAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATg                                                <  1:1738861/71‑1 (MQ=255)                   cgcAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATg                                                <  1:908132/71‑1 (MQ=255)                    gcAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGa                                               <  1:4741682/71‑1 (MQ=255)                    gcAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGa                                               <  1:1164305/71‑1 (MQ=255)                        aaaGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGtatta                                                        >  1:3652558/1‑58 (MQ=255)                         aaGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGaa                                                                >  1:4721063/1‑49 (MQ=255)                          aGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGtatta                                                        >  1:2689715/1‑56 (MQ=255)                          aGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGc                                         >  1:215755/1‑71 (MQ=255)                           gCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGcc                                        >  1:4672137/1‑71 (MQ=255)                             tGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCt                                      >  1:5298725/1‑71 (MQ=255)                               tttACCCGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGc                                                    <  1:916949/53‑1 (MQ=255)                               tttACCCGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATg                                                <  1:904699/57‑1 (MQ=255)                                ttACCCGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGtatta                                                        >  1:4626789/2‑50 (MQ=255)                                ttACCCGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCg                                                   <  1:4802785/54‑1 (MQ=255)                                ttACCCGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCg                                                   <  1:3885847/54‑1 (MQ=255)                                 tACCCGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACa                                                              >  1:1987958/1‑43 (MQ=12)                                 tACCCGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGtt                                   <  1:5211454/70‑1 (MQ=255)                                 tACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTc                                  >  1:789441/1‑71 (MQ=255)                                  aCCCGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGAt                                                 >  1:3713791/1‑55 (MQ=255)                                  aCCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTc                                  >  1:276759/1‑70 (MQ=255)                                  aCCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTc                                  >  1:3480601/1‑70 (MQ=255)                                    ccGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGtttg                                 >  1:930567/1‑67 (MQ=255)                                    ccGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGt                                                            <  1:4177020/42‑1 (MQ=255)                                    ccGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGt                                                            <  1:2601327/42‑1 (MQ=255)                                    ccGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGt                                                            <  1:3013033/42‑1 (MQ=255)                                     cGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGtatta                                                        >  1:1030782/1‑45 (MQ=12)                                     cGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATg                                                <  1:1411955/53‑1 (MQ=255)                                     cGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATg                                                <  1:5291644/53‑1 (MQ=255)                                     cGCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGtttgg                                >  1:582751/1‑66 (MQ=255)                                     cGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGAt                               >  1:399332/1‑70 (MQ=255)                                      gCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTaa                             <  1:4646674/71‑1 (MQ=255)                                       ccTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATg                                                <  1:3888036/51‑1 (MQ=255)                                       ccTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATg                                                <  1:3169322/51‑1 (MQ=255)                                       ccTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATaaa                            <  1:3471370/71‑1 (MQ=255)                                          ggCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGAc                         >  1:622087/1‑71 (MQ=255)                                           gCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTaaacat                         <  1:2447591/70‑4 (MQ=255)                                           gCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGa                          <  1:5044865/69‑1 (MQ=255)                                            ccGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGa                       <  1:212857/71‑1 (MQ=255)                                              gCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGaa                                              >  1:3186978/1‑46 (MQ=16)                                              gCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATc                     <  1:1670750/71‑1 (MQ=255)                                              gCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATc                     >  1:5469915/1‑71 (MQ=255)                                                aCCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCgg                   >  1:3774442/1‑71 (MQ=255)                                                 gcTTGGCGGTAAAGTTGTCTATGATCAGTATTATGGCGATg                                                <  1:446047/40‑1 (MQ=25)                                                 ccTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCgg                   >  1:1661023/1‑70 (MQ=255)                                                  cTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGccc                                       >  1:2790648/1‑49 (MQ=255)                                                  cTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATa                              <  1:4725793/58‑1 (MQ=255)                                                  cTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATa                              <  1:5462014/58‑1 (MQ=255)                                                      gCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAg              <  1:5163512/70‑1 (MQ=255)                                                      gCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAg              <  1:4415364/70‑1 (MQ=255)                                                       cGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTa            <  1:4158708/71‑1 (MQ=255)                                                         gTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGt                                    <  1:3053862/45‑1 (MQ=255)                                                         gTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGt                                    <  1:74015/45‑1 (MQ=255)                                                         gTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAAt          >  1:4577140/1‑71 (MQ=255)                                                          tAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTg                                     <  1:4469042/43‑1 (MQ=255)                                                           aaaCTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTc                                  >  1:4641011/1‑45 (MQ=255)                                                           aaaCTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCaaa               >  1:3612501/1‑64 (MQ=255)                                                           aaaCTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATcc        >  1:4000099/1‑71 (MQ=255)                                                           aaaCTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATcc        >  1:3678873/1‑71 (MQ=255)                                                            aaCTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGtt                                   >  1:1547429/1‑43 (MQ=255)                                                                ggTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATg   >  1:5237960/1‑71 (MQ=255)                                                                 gTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGc  <  1:713950/71‑1 (MQ=255)                                                                  tCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCa                 <  1:4801790/55‑1 (MQ=255)                                                                  tCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCa                 <  1:5048629/55‑1 (MQ=255)                                                                   cTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGc  >  1:2481538/1‑69 (MQ=255)                                                                   |                                                                     CAATGGCTGGGATGTACGCGCAGAAAGCTGGCTACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGC  >  Exported/1279160‑1279294

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑