Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,279,262 C→T 11.7% F347F (TTC→TTT yeeJ → adhesin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,279,2620CT11.7% 229.5 / 15.8 85F347F (TTC→TTTyeeJadhesin
Reads supporting (aligned to +/- strand):  ref base C (45/30);  new base T (7/3);  total (52/33)
Fisher's exact test for biased strand distribution p-value = 7.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.37e-01

TACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTATACCCCCTTCCCGCTGA  >  Exported/1279192‑1279332
                                                                      |                                                                      
tACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTc                                                                        >  1:789441/1‑71 (MQ=255)
 aCCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTc                                                                        >  1:276759/1‑70 (MQ=255)
 aCCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTc                                                                        >  1:3480601/1‑70 (MQ=255)
    cGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGAt                                                                     >  1:399332/1‑70 (MQ=255)
     gCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTaa                                                                   <  1:4646674/71‑1 (MQ=255)
      ccTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATaaa                                                                  <  1:3471370/71‑1 (MQ=255)
         ggCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGAc                                                               >  1:622087/1‑71 (MQ=255)
          gCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTaaacat                                                               <  1:2447591/70‑4 (MQ=255)
          gCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGa                                                                <  1:5044865/69‑1 (MQ=255)
           ccGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGa                                                             <  1:212857/71‑1 (MQ=255)
             gCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATc                                                           <  1:1670750/71‑1 (MQ=255)
             gCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATc                                                           >  1:5469915/1‑71 (MQ=255)
               aCCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCgg                                                         >  1:3774442/1‑71 (MQ=255)
                ccTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCgg                                                         >  1:1661023/1‑70 (MQ=255)
                 cTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATa                                                                    <  1:5462014/58‑1 (MQ=255)
                 cTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATa                                                                    <  1:4725793/58‑1 (MQ=255)
                     gCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAg                                                    <  1:4415364/70‑1 (MQ=255)
                     gCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAg                                                    <  1:5163512/70‑1 (MQ=255)
                      cGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTa                                                  <  1:4158708/71‑1 (MQ=255)
                        gTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAAt                                                >  1:4577140/1‑71 (MQ=255)
                          aaaCTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTc                                                                        >  1:4641011/1‑45 (MQ=255)
                          aaaCTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCaaa                                                     >  1:3612501/1‑64 (MQ=255)
                          aaaCTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATcc                                              >  1:4000099/1‑71 (MQ=255)
                          aaaCTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATcc                                              >  1:3678873/1‑71 (MQ=255)
                               ggTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATg                                         >  1:5237960/1‑71 (MQ=255)
                                gTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGc                                        <  1:713950/71‑1 (MQ=255)
                                 tCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCa                                                       <  1:4801790/55‑1 (MQ=255)
                                 tCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCa                                                       <  1:5048629/55‑1 (MQ=255)
                                  cTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGc                                        >  1:2481538/1‑69 (MQ=255)
                                   tATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGa                                                             <  1:4473728/47‑1 (MQ=255)
                                   tATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGa                                                             <  1:1009565/47‑1 (MQ=255)
                                   tATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTa                                                  <  1:1886378/58‑1 (MQ=255)
                                     tGAACAGTATTATGGCTATGAAGTCGGGCTGTTTGGTaaa                                                                  >  1:947842/1‑40 (MQ=16)
                                     tGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGc                                                        >  1:132388/1‑50 (MQ=255)
                                     tGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATaa                                   >  1:1902824/1‑71 (MQ=255)
                                     tGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATa                                    <  1:4411527/70‑1 (MQ=255)
                                     tGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTaaa                                                                  >  1:247319/1‑40 (MQ=16)
                                     tGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTaaa                                                                  >  1:4522852/1‑40 (MQ=16)
                                     tGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTaaa                                                                  >  1:644916/1‑40 (MQ=16)
                                     tGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTaaa                                                                  >  1:5562060/1‑40 (MQ=16)
                                     tGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGa                                                                >  1:547210/1‑42 (MQ=19)
                                     tGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGa                                                                >  1:3504138/1‑42 (MQ=19)
                                      gAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACgct                                                            >  1:1865255/1‑43 (MQ=255)
                                      gAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCaaa                                                     >  1:5495788/1‑52 (MQ=255)
                                      gAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCaaa                                                     >  1:4982896/1‑52 (MQ=255)
                                      gAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCaaa                                                     >  1:1861234/1‑52 (MQ=255)
                                       aaCAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGa                                                             >  1:1065708/1‑43 (MQ=255)
                                       aaCAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGAt                                                            >  1:1696726/1‑44 (MQ=255)
                                       aaCAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGAt                                                            >  1:390284/1‑44 (MQ=255)
                                       aaCAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAAcc                                 >  1:3649832/1‑71 (MQ=255)
                                        aCAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCg                                <  1:2886515/71‑1 (MQ=255)
                                           gTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCa                                           <  1:3520479/57‑1 (MQ=255)
                                             attatGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCg                                                          >  1:1046219/1‑40 (MQ=255)
                                             attatGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCg                                                          >  1:3319100/1‑40 (MQ=255)
                                              ttatGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCa                                           >  1:1897872/1‑54 (MQ=255)
                                              ttatGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACGGCTgg                            <  1:2634890/69‑1 (MQ=255)
                                              ttatGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTgg                            <  1:3714117/69‑1 (MQ=255)
                                              ttatGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTgg                            <  1:690727/69‑1 (MQ=255)
                                              ttatGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGAc                          <  1:409899/71‑1 (MQ=255)
                                              ttatGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGAc                          >  1:3419483/1‑71 (MQ=255)
                                               tatGGCCATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATaa                                   >  1:4468066/1‑61 (MQ=255)
                                                   gCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAAc                     <  1:795407/71‑1 (MQ=255)
                                                    cGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAAc                     >  1:3659153/1‑70 (MQ=255)
                                                     gATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACta                   >  1:260368/1‑71 (MQ=255)
                                                      aTGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTaa                                                 <  1:4151459/40‑1 (MQ=255)
                                                       tGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACtata                 <  1:1159844/71‑1 (MQ=255)
                                                        gAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCa                                      >  1:773287/1‑49 (MQ=255)
                                                        gAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCa                                      >  1:5288081/1‑49 (MQ=255)
                                                        gAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCa                                      >  1:1669836/1‑49 (MQ=255)
                                                        gAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTATAc                >  1:4278948/1‑71 (MQ=255)
                                                           gTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTATAcccc             >  1:3522912/1‑71 (MQ=255)
                                                            tGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTc                                            >  1:1573677/1‑39 (MQ=255)
                                                            tGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTg                             <  1:1941665/54‑1 (MQ=255)
                                                            tGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTATAccccc            >  1:5304496/1‑71 (MQ=255)
                                                               cccTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACta                   >  1:3542256/1‑61 (MQ=255)
                                                                 cTGTTCGATAAAGACTATCGGCAAAGTAATCCTCATg                                         <  1:2206558/37‑1 (MQ=255)
                                                                 cTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTATACCCCCTTccc       >  1:5117757/1‑71 (MQ=255)
                                                                 cTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTATACCCCCTTccc       <  1:3262022/71‑1 (MQ=255)
                                                                  tGTTCGATAAAGACGATCGGCAAAGTAATCCTCATg                                         <  1:3521756/36‑1 (MQ=255)
                                                                  tGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTATACCCCCTTCCCg      >  1:3563214/1‑71 (MQ=255)
                                                                   gTTCGATAAAGACGATCGGCAAAGTAATCCTCATGcc                                       >  1:2481499/1‑37 (MQ=255)
                                                                   gTTCGATAAAGACGATCGGCAAAGTAATCCTCATGcc                                       >  1:5581572/1‑37 (MQ=255)
                                                                   gTTCGATAAAGACGATCGGCAAAGTAATCCTCATGcc                                       >  1:3492291/1‑37 (MQ=255)
                                                                   gTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCAt                                     <  1:4838388/39‑1 (MQ=255)
                                                                   gTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAAc                     <  1:5158740/55‑1 (MQ=255)
                                                                    ttCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATa                                    >  1:2539876/1‑39 (MQ=255)
                                                                    ttCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATa                                    >  1:1324100/1‑39 (MQ=255)
                                                                    ttCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAAc                                  >  1:2315906/1‑41 (MQ=255)
                                                                     tCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTATACCCCCTTCCCGCt    >  1:3023953/1‑70 (MQ=255)
                                                                      cGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCt                              >  1:1756323/1‑43 (MQ=255)
                                                                      cGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTaa                      >  1:5602586/1‑51 (MQ=255)
                                                                      cGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTa                       <  1:4230057/50‑1 (MQ=255)
                                                                      cGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTATAccccc            >  1:1101913/1‑61 (MQ=255)
                                                                      cGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTATACCCCCTTCCCGCtga  >  1:3359988/1‑71 (MQ=255)
                                                                      cGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTATACCCCCTTCCCGCtga  >  1:3366479/1‑71 (MQ=255)
                                                                      |                                                                      
TACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTATACCCCCTTCCCGCTGA  >  Exported/1279192‑1279332

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: