Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | Exported | 1,279,262 | C→T | 11.7% | F347F (TTC→TTT) | yeeJ → | adhesin |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | Exported | 1,279,262 | 0 | C | T | 11.7% | 229.5 / 15.8 | 85 | F347F (TTC→TTT) | yeeJ | adhesin |
Reads supporting (aligned to +/- strand): ref base C (45/30); new base T (7/3); total (52/33) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 7.34e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.37e-01 |
TACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTATACCCCCTTCCCGCTGA > Exported/1279192‑1279332 | tACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTc > 1:789441/1‑71 (MQ=255) aCCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTc > 1:276759/1‑70 (MQ=255) aCCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTc > 1:3480601/1‑70 (MQ=255) cGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGAt > 1:399332/1‑70 (MQ=255) gCCTGGCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTaa < 1:4646674/71‑1 (MQ=255) ccTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATaaa < 1:3471370/71‑1 (MQ=255) ggCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGAc > 1:622087/1‑71 (MQ=255) gCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTaaacat < 1:2447591/70‑4 (MQ=255) gCCGCAGCTTGGCGCAAGCCTGATGTATGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGa < 1:5044865/69‑1 (MQ=255) ccGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGa < 1:212857/71‑1 (MQ=255) gCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATc < 1:1670750/71‑1 (MQ=255) gCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATc > 1:5469915/1‑71 (MQ=255) aCCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCgg > 1:3774442/1‑71 (MQ=255) ccTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCgg > 1:1661023/1‑70 (MQ=255) cTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATa < 1:5462014/58‑1 (MQ=255) cTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATa < 1:4725793/58‑1 (MQ=255) gCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAg < 1:4415364/70‑1 (MQ=255) gCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAg < 1:5163512/70‑1 (MQ=255) cGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTa < 1:4158708/71‑1 (MQ=255) gTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAAt > 1:4577140/1‑71 (MQ=255) aaaCTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTc > 1:4641011/1‑45 (MQ=255) aaaCTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCaaa > 1:3612501/1‑64 (MQ=255) aaaCTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATcc > 1:4000099/1‑71 (MQ=255) aaaCTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATcc > 1:3678873/1‑71 (MQ=255) ggTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATg > 1:5237960/1‑71 (MQ=255) gTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGc < 1:713950/71‑1 (MQ=255) tCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCa < 1:4801790/55‑1 (MQ=255) tCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCa < 1:5048629/55‑1 (MQ=255) cTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGc > 1:2481538/1‑69 (MQ=255) tATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGa < 1:4473728/47‑1 (MQ=255) tATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGa < 1:1009565/47‑1 (MQ=255) tATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTa < 1:1886378/58‑1 (MQ=255) tGAACAGTATTATGGCTATGAAGTCGGGCTGTTTGGTaaa > 1:947842/1‑40 (MQ=16) tGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGc > 1:132388/1‑50 (MQ=255) tGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATaa > 1:1902824/1‑71 (MQ=255) tGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATa < 1:4411527/70‑1 (MQ=255) tGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTaaa > 1:247319/1‑40 (MQ=16) tGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTaaa > 1:4522852/1‑40 (MQ=16) tGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTaaa > 1:644916/1‑40 (MQ=16) tGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTaaa > 1:5562060/1‑40 (MQ=16) tGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGa > 1:547210/1‑42 (MQ=19) tGAACAGTATTATGGCGATGAAGTCGGGCTGTTTGGTAAAGa > 1:3504138/1‑42 (MQ=19) gAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACgct > 1:1865255/1‑43 (MQ=255) gAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCaaa > 1:5495788/1‑52 (MQ=255) gAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCaaa > 1:4982896/1‑52 (MQ=255) gAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCaaa > 1:1861234/1‑52 (MQ=255) aaCAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGa > 1:1065708/1‑43 (MQ=255) aaCAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGAt > 1:1696726/1‑44 (MQ=255) aaCAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGAt > 1:390284/1‑44 (MQ=255) aaCAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAAcc > 1:3649832/1‑71 (MQ=255) aCAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCg < 1:2886515/71‑1 (MQ=255) gTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCa < 1:3520479/57‑1 (MQ=255) attatGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCg > 1:1046219/1‑40 (MQ=255) attatGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCg > 1:3319100/1‑40 (MQ=255) ttatGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCa > 1:1897872/1‑54 (MQ=255) ttatGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACGGCTgg < 1:2634890/69‑1 (MQ=255) ttatGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTgg < 1:3714117/69‑1 (MQ=255) ttatGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTgg < 1:690727/69‑1 (MQ=255) ttatGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGAc < 1:409899/71‑1 (MQ=255) ttatGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGAc > 1:3419483/1‑71 (MQ=255) tatGGCCATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATaa > 1:4468066/1‑61 (MQ=255) gCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAAc < 1:795407/71‑1 (MQ=255) cGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAAc > 1:3659153/1‑70 (MQ=255) gATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACta > 1:260368/1‑71 (MQ=255) aTGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTaa < 1:4151459/40‑1 (MQ=255) tGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACtata < 1:1159844/71‑1 (MQ=255) gAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCa > 1:773287/1‑49 (MQ=255) gAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCa > 1:5288081/1‑49 (MQ=255) gAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCa > 1:1669836/1‑49 (MQ=255) gAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTATAc > 1:4278948/1‑71 (MQ=255) gTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTATAcccc > 1:3522912/1‑71 (MQ=255) tGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTc > 1:1573677/1‑39 (MQ=255) tGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTg < 1:1941665/54‑1 (MQ=255) tGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTATAccccc > 1:5304496/1‑71 (MQ=255) cccTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACta > 1:3542256/1‑61 (MQ=255) cTGTTCGATAAAGACTATCGGCAAAGTAATCCTCATg < 1:2206558/37‑1 (MQ=255) cTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTATACCCCCTTccc > 1:5117757/1‑71 (MQ=255) cTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTATACCCCCTTccc < 1:3262022/71‑1 (MQ=255) tGTTCGATAAAGACGATCGGCAAAGTAATCCTCATg < 1:3521756/36‑1 (MQ=255) tGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTATACCCCCTTCCCg > 1:3563214/1‑71 (MQ=255) gTTCGATAAAGACGATCGGCAAAGTAATCCTCATGcc > 1:2481499/1‑37 (MQ=255) gTTCGATAAAGACGATCGGCAAAGTAATCCTCATGcc > 1:5581572/1‑37 (MQ=255) gTTCGATAAAGACGATCGGCAAAGTAATCCTCATGcc > 1:3492291/1‑37 (MQ=255) gTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCAt < 1:4838388/39‑1 (MQ=255) gTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAAc < 1:5158740/55‑1 (MQ=255) ttCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATa > 1:2539876/1‑39 (MQ=255) ttCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATa > 1:1324100/1‑39 (MQ=255) ttCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAAc > 1:2315906/1‑41 (MQ=255) tCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTATACCCCCTTCCCGCt > 1:3023953/1‑70 (MQ=255) cGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCt > 1:1756323/1‑43 (MQ=255) cGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTaa > 1:5602586/1‑51 (MQ=255) cGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTa < 1:4230057/50‑1 (MQ=255) cGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTATAccccc > 1:1101913/1‑61 (MQ=255) cGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTATACCCCCTTCCCGCtga > 1:3359988/1‑71 (MQ=255) cGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTATACCCCCTTCCCGCtga > 1:3366479/1‑71 (MQ=255) | TACCCGCCTGGCCGCACCTTGGCGGTAAACTGGTCTATGAACAGTATTATGGCGATGAAGTGGCCCTGTTCGATAAAGACGATCGGCAAAGTAATCCTCATGCCATAACCGCTGGACTTAACTATACCCCCTTCCCGCTGA > Exported/1279192‑1279332 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |