| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,306,778 | T→A | 6.9% | Q273L (CAG→CTG) | yeeF ← | predicted amino‑acid transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,306,778 | 0 | T | A | 6.9% | 244.5 / 8.4 | 87 | Q273L (CAG→CTG) | yeeF | predicted amino‑acid transporter |
| Reads supporting (aligned to +/- strand): ref base T (37/44); new base A (2/4); total (39/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.87e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01 | |||||||||||
GTAATGGTGGAGAAGATCAGCGCGCCAACCTGGAACGCTTTACCCGCAACGTACAGCATGATTTCAGGCTGTGACGCATCCGGATCTTTAAAGCGAGAGATATCCGGGAAGTACAGCTGCAGGAAGTAAGTTGCAAA > Exported/1306709‑1306845 | gTAATGGTGGAGAAGATCAGCGCGCCAACCTGGAACGCTTTACCCGCAACGTACAGCATGATTTCAGGCtg > 1:2061801/1‑71 (MQ=255)gTAATGGTGGAGAAGATCAGCGCGCCAACCTGGAACGCTTTACCCGCAACGTACAGCATGATTTCAGGCtg > 1:2695795/1‑71 (MQ=255) tAATGGTGGAGAAGATCAGCGCGCCAACCTGGAACGCTTTACCCGCAACGTACAGCATGATTTCAGGCtgt > 1:2112337/1‑71 (MQ=255) tAATGGTGGAGAAGATCAGCGCGCCAACCTGGAACGCTTTACCCGCAACGTACAGCATGATTTCAGGCtgt < 1:2512543/71‑1 (MQ=255) tAATGGTGGAGAAGATCAGCGCGCCAACCTGGAACGCTTTACCCGCAACGTACAGCATGATTTCAGGCtgt < 1:2054760/71‑1 (MQ=255) tAATGGTGGAGAAGATCAGCGCGCCAACCTGGAACGCTTTACCCGCAACGTACAGCATGATTTCAGGCtgt < 1:3497764/71‑1 (MQ=255) aTGGTGGAGAAGATCAGCGCGCCAACCTGGAACGCTTTACCCGCAACGTACAGCATGATTTCAGGCTGTGa > 1:1193226/1‑71 (MQ=255) aTGGTGGAGAAGATCAGCGCGCCAACCTGGAACGCTTTACCCGCAACGTACAGCATGATTTCAGGCTGTGa > 1:1480212/1‑71 (MQ=255) gtggAGAAGATCAGCGCGCCAACCTGGAACGCTTTACCCGCAACGTACAGCATGATTTCAGGCTGTGACg < 1:1123497/70‑1 (MQ=255) tggAGAAGATCAGCGCGCCAACCTGGAACGCTTTACCCGCAACGTACAGCATGATTTCAGGCTGTGACGCa < 1:2756389/71‑1 (MQ=255) ggAGAAGATCAGCGCGCCAACCTGGAACGCTTTACCCGCAACGTACAGCATGATTTCAGGCTGTGACGCa > 1:1230393/1‑70 (MQ=255) ggAGAAGATCAGCGCGCCAACCTGGAACGCTTTACCCGCAACGTACAGCATGATTTCAGGCTGTGACGCAt > 1:4488377/1‑71 (MQ=255) gagaAGATCAGCGCGCCAACCTGGAACGCTTTACCCGCAACGTACAGCATGATTTCAGGCTGTGACGCAt < 1:5549511/70‑1 (MQ=255) gagaAGATCAGCGCGCCAACCTGGAACGCTTTACCCGCAACGTACAGCATGATTTCAGGCTGTGACGCAt < 1:222983/70‑1 (MQ=255) agaagaTCAGCGCGCCAACCTGGAACGCTTTACCCGCAACGTACAGCATGATTTCAGGCTGTGACGCATcc < 1:1815106/71‑1 (MQ=255) gaagaTCAGCGCGCCAACCTGGAACTCTTTACCCGCAACGTACAGCATGATTTCAGGCtgt < 1:3157770/61‑1 (MQ=255) aagaTCAGCGCGCCAACCTGGAACGCTTTACCCGCAACGTACAGCATGATTTCAGGCTGTGACGCATCCg > 1:3765504/1‑70 (MQ=255) aagaTCAGCGCGCCAACCTGGAACGCTTTACCCGCAACGTACAGCATGATTTCAGGCTGTGACGCATCCg > 1:1789956/1‑70 (MQ=255) gaTCAGCGCGCCAACCTGGAACGCTTTACCCGCAACGTACAGCATGATTTCAGGCTGTGa > 1:4780771/1‑60 (MQ=255) gaTCAGCGCGCCAACCTGGAACGCTTTACCCGCAACGTACAGCATGATTTCAGGCTGTGa > 1:4963932/1‑60 (MQ=255) aTCAGCGCGCCAACCTGGAACGCTTTACCCGCAACGTACAGCATGATTTCAGGCt < 1:5399209/55‑1 (MQ=255) tCAGCGCGCCAACCTGGAACGCTTTACCCGCAACGTACAGCATGATTTCAGGCTGTGa > 1:1318303/1‑58 (MQ=255) tCAGCGCGCCAACCTGGAACGCTTTACCCGCAACGTACAGCATGATTTCAGGCTGTGa > 1:4011662/1‑58 (MQ=255) tCAGCGCGCCAACCTGGAACGCTTTACCCGCAACGTACAGCATGATTTCAGGCTGTGACGCATCCGGATCt < 1:3294005/71‑1 (MQ=255) tCAGCGCGCCAACCTGGAACGCTTTACCCGCAACGTACAGCATGATTTCAGGCTGTGACGCATCCGGATCt < 1:2426017/71‑1 (MQ=255) tCAGCGCGCCAACCTGGAACGCTTTACCCGCAACGTACAGCATGATTTCAGGCTGTGACGCATCCGGATCt > 1:5467076/1‑71 (MQ=255) cAGCGCGCCAACCTGGAACGCTTTACCCGCAACGTACAGCATGATTTCAGGCtgt > 1:3677330/1‑55 (MQ=255) cAGCGCGCCAACCTGGAACGCTTTACCCGCAACGTACAGCATGATTTCAGGCtgt > 1:4413965/1‑55 (MQ=255) cAGCGCGCCAACCTGGAACGCTTTACCCGCAACGTACAGCATGATTTCAGGCTGTGAc > 1:2743283/1‑58 (MQ=255) cAGCGCGCCAACCTGGAACGCTTTACCCGCAACGTACAGCATGATTTCAGGCTGTGACGCATCCGGATCtt > 1:1264537/1‑71 (MQ=255) cAGCGCGCCAACCTGGAACGCTTTACCCGCAACGTACAGCATGATATCAGGCTGTGACGCATCCGGATCtt > 1:5058342/1‑71 (MQ=255) aGCGCGCCAACCTGGAACGCTTTACCCGCAACGTACAGCATGATTTCAGGCTGTGACGCATCCGGATCtt < 1:3565814/70‑1 (MQ=255) gcgcgcCAACCTGGAACGCTTTACCCGCAACGTACAGCATGATTTCAGGCTGTGACGCATCCGGATCTTTa < 1:173454/71‑1 (MQ=255) gcgcgcCAACCTGGAACGCTTTACCCGCAACGTACAGCATGATTTCAGGCTGTGACGCATCCGGATCTTTa < 1:677494/71‑1 (MQ=255) gcgcgcCAACCTGGAACGCTTTACCCGCAACGTACAGCATGATTTCAGGCTGTGACGCATCCGGATCTTTa < 1:3053742/71‑1 (MQ=255) cgcgcCAACCTGGAACGCTTTACCCGCAACGTACAGCATGATTTCAGGCTGTGACGCATCCGGATCtt < 1:1339076/68‑1 (MQ=255) gcCAACCTGGAACGCTTTACCCGCAACGTACAGCATGATTTCAGGCTGTGACGCATCCGGATCTTTAAAGc < 1:755550/71‑1 (MQ=255) gcCAACCTGGAACGCTTTACCCGCAACGTACAGCATGATTTCAGGCTGTGACGCATCCGGATCTTTAAAGc > 1:3107298/1‑71 (MQ=255) aCCTGGAACGCTTTACCCGCAACGTACAGCATGATTTCAGGCTGTGACGCATCCGGATCTTTAAAGCgaga < 1:2673531/71‑1 (MQ=255) aCCTGGAACGCTTTACCCGCAACGTACAGCATGATTTCAGGCTGTGACGCATCCGGATCTTTAAAGCgaga < 1:3553011/71‑1 (MQ=255) aCCTGGAACGCTTTACCCGCAACGTACAGCATGATTTCAGGCTGTGACGCATCCGGATCTTTAAAGCga > 1:1800034/1‑69 (MQ=255) ccTGGAACGCTTTACCCGCAACGTACAGCATGATTTCAGGCTGTGACGCATCCGGATCTTTAAAGCgagag < 1:3851691/71‑1 (MQ=255) ccTGGAACGCTTTACCCGCAACGTACAGCATGATTTCAGGCTGTGACGCATCCGGATCTTTAAAGCga > 1:5546926/1‑68 (MQ=255) tGGAACGCTTTACCCGCAACGTACAGCATGATTTCAGGCTGTGa > 1:4916751/1‑44 (MQ=255) gAACGCTTTACCCGCAACGTACAGCATGATTTCAGGCTGTGACGCATCCgg < 1:2308041/51‑1 (MQ=255) gAACGCTTTACCCGCAACGTACAGCATGATTTCAGGCTGTGACGCATCCGGATCTTTAAAGCGAGAGatat > 1:1236384/1‑71 (MQ=255) gAACGCTTTACCCGCAACGTACAGCATGATTTCAGGCTGTGACGCATCCGGATCTTTAAAGCGAGAGata < 1:573287/70‑1 (MQ=255) gAACGCTTTACCCGCAACGTACAGCATGATTTCAGGCTGTGACGCATCCGGATCTTTAAAGCGAGAGata < 1:4785482/70‑1 (MQ=255) aaCGCTTTACCCGCAACGTACAGCATGATTTCAGGCTGTGACGCATCCGGATCTTTa < 1:2833651/57‑1 (MQ=255) aCGCTTTACCCGCAACGTACAGCATGATTTCAGGCTGTGACGCATCCGGATCTTTAAAGCGAGAGATATcc > 1:162130/1‑71 (MQ=255) aCGCTTTACCCGCAACGTACAGCATGATTTCAGGCTGTGACGCATCCGGATCTTTAAAGCGAGAGATATcc > 1:5049038/1‑71 (MQ=255) cGCTTTACCCGCAACGTACAGCATGATTTCAGGCTGTGACGCATCCGGATCTTTAAAGCGAGAGATATCCg > 1:3102021/1‑71 (MQ=255) gCTTTACCCGCAACGTACAGCATGATTTCAGGCTGTGACGCATCCGGATCtt < 1:532952/52‑1 (MQ=255) cTTTACCCGCAACGTACAGCATGATTTCAGGCTGTGACGCATCCGGATCTTTAAAGCGAGAGATATCCggg < 1:822299/71‑1 (MQ=255) cTTTACCCGCAACGTACAGCATGATTTCAGGCTGTGACGCATCCGGATCTTTAAAGCGAGAGATATCCggg < 1:4552386/71‑1 (MQ=255) cTTTACCCGCAACGTACAGCATGATTTCAGGCTGTGACGCATCCGGATCTTTAAAGCGAGAGATATCCgg > 1:4128342/1‑70 (MQ=255) tttACCCGCAACGTACAGCATGATTTCAGGCTGTGACGCAt < 1:400819/41‑1 (MQ=255) tACCCGCAACGTACAGCATGATTTCAGGCTGTGACGCATCCGGATCTTTAAAGCGAGAGATATCCGGGAAg > 1:4307789/1‑71 (MQ=255) aCCCGCAACGTACAGCATGATTTCAGGCTGTGACGCATCCGGATCTTTAAAGCGAGAGATATCCGGGa > 1:2237705/1‑68 (MQ=255) cccGCAACGTACAGCATGATTTCAGGCTGTGACGCATCCGGATCTTTAAAGCGAGAGATATCCGGGAAGt < 1:2705401/70‑1 (MQ=255) cccGCAACGTACAGCATGATTTCAGGCTGTGACGCATCCGGATCTTTAAAGCGAGAGATATCCGGGAAGt < 1:5312918/70‑1 (MQ=255) cccGCAACGTACAGCATGATTTCAGGCTGTGACGCATCCGGATCTTTAAAGCGAGAGATATCCGGGAAGt < 1:3252854/70‑1 (MQ=255) ccGCAACGTACAGCATGATTTCAGGCTGTGACGCATCCGGATCTTTAAAGCGAGAGATATCCGGGAAGTAc < 1:1880987/71‑1 (MQ=255) gCAACGTACAGCATGATTTCAGGCTGTGACGCATCCGGATCTTTAAAGCGAGAGATATCCGGGAAGTACAg > 1:2175576/1‑71 (MQ=255) ccgaCGTACAGCGCAATTTCAGGCAGTGCGGCATCCGGATCTTTAAAGCGGCTGATATCGGGAAAGAACAg > 1:822451/4‑71 (MQ=255) cAACGTACAGCATGATTTCAGGCTGTGACGCATCCGGATCTTTAAAGCGAGAGATATcc > 1:890862/1‑59 (MQ=255) cAACGTACAGCATGATTTCAGGCTGTGACGCATCCGGATCTTTAAAGCGAGAGATATcc > 1:3197634/1‑59 (MQ=255) cAACGTACAGCATGATTTCAGGCTGTGACGCATCCGGATCTTTAAAGCGAGAGATATcc > 1:1540982/1‑59 (MQ=255) gaCGTACAGCGCAATTTCAGGCAGTGCGGCATCCGGATCTTTAAAGCGGCTGATATCGGGAAAGAACAGCt < 1:2339057/70‑1 (MQ=255) gaCGTACAGCGCAATTTCAGGCAGTGCGGCATCCGGATCTTTAAAGCGGCTGATATCGGGAAAGAACAGCt < 1:5173562/70‑1 (MQ=255) aaCGTACAGCATGATTTCAGGCTGTGACGCATCCGGATCTTTAAAGc < 1:2570847/47‑1 (MQ=255) aCGTACAGCGCAATTTCAGGCAGTGCGGCATCCGGATCTTTAAAGCGGCTGATATcgg < 1:5658846/58‑3 (MQ=255) aCGTACAGCATGATTTCAGGCTGTGACGCATCCGGATCTTTAAAGCGAGAGATATCCGGGAAGTACAGCTg < 1:5211381/71‑1 (MQ=255) cGTACAGCGCAATTTCAGGCAGTGCGGCATCCGGATCTTTAAAGCGGCTGATATCGGGAAAGAACACCt < 1:5491982/69‑1 (MQ=255) cGTACAGCATGATTTCAGGCTGTGACGCATCCGGATCTTTa < 1:4716481/41‑1 (MQ=255) cGTACAGCATGATTTCAGGCTGTGACGCATCCGGATCTTTAAAGCGAGAGata > 1:1467903/1‑53 (MQ=255) gTACAGCATGATTTCAGGCTGTGACGCATCCGGATCTTTAAAGCGAGAGATATCCGGGAAGTACAGCTg > 1:932665/1‑69 (MQ=255) gTACAGCATGATTTCAGGCTGTGACGCATCCGGATCTTTAAAGCGAGAGATATCCGGGAAGTACAGCTg > 1:4251213/1‑69 (MQ=255) tACAGCATGATTTCAGGCTGTGACGCATCCGGATCTTTAAAGCGAGAGATATCCGGGAAGTa > 1:2815789/1‑62 (MQ=255) cAGCATGATTTCAGGCTGTGACGCATCCGGATCTTTAAAGCGAGAGATATCCg > 1:5664669/1‑53 (MQ=255) cAGCATGATTTCAGGCTGTGACGCATCCGGATCTTTAAAGCGAGAGATATCCGGGAAGTACAGCTGCAgg < 1:5340198/70‑1 (MQ=255) cAGCATGATTTCAGGCTGTGACGCATCCGGATCTTTAAAGCGAGAGATATCCGGGAAGTACAGCTGCAGGa < 1:592404/71‑1 (MQ=255) cATGATTTCAGGCTGTGACGCATCCGGATCTTTAAAGCGAGAGATATCCggg < 1:1238806/52‑1 (MQ=255) cATGATTTCAGGCTGTGACGCATCCGGATCTTTAAAGCGAGAGATATCCg < 1:3252306/50‑1 (MQ=255) cATGATTTCAGGCTGTGACGCATCCGGATCTTTAAAGCGAGAGATATCCGGGAAGTACAGCTGCAGGaagt < 1:2356876/71‑1 (MQ=255) aTGATTTCAGGCTGTGACGCATCCGGATCTTTAAAGCGAGAGATATCCGGGAAGTACAGCTGCAGGaagta < 1:1585551/71‑1 (MQ=255) aTGATTTCAGGCTGTGACGCATCCGGATCTTTAAAGCGAGAGATATCCGGGAAGTACAGCTGCAGGaagta < 1:3349690/71‑1 (MQ=255) aTGATATCAGGCTGTGACGCATCCGGATCTTTAAAGCGAGAGatat > 1:1107097/1‑46 (MQ=255) tGATTTCAGGCTGTGACGCATCCGGATCTTTAAAGc < 1:5419057/36‑1 (MQ=255) tGATTTCAGGCTGTGACGCATCCGGATCTTTAAAGCGAGAGATATCCGGGAAGTACAGCTGCAGGaagtaa < 1:1787441/71‑1 (MQ=255) aaTTTCAGGCAGTGCGGCATCCGGATCTTTAAAGCGGCTGATATc > 1:1029696/2‑45 (MQ=255) tttCAGGCTGTGACGCATCCGGATCTTTAAAGCGAGAGATATcc < 1:4894783/44‑1 (MQ=255) ttCAGGCTGTGACGCATCCGGATCTTTAAAGCGAGAGATATcc < 1:1675781/43‑1 (MQ=255) tCAGGCTGTGACGCATCCGGATCTTTAAAGCGAGAGATATCCGGGAAGTACAGCTGCAGGAAGTAAGTTg > 1:2886708/1‑70 (MQ=255) tCAGGCTGTGACGCATCCGGATCTTTAAAGCGAGAGATATCCGGGAAGTACAGCTGCAGGAAGTAAGTTg > 1:3531869/1‑70 (MQ=255) aGGCTGTGACGCATCCGGATCTTTAAAGCGAGAGATATCCGGGAAGTACAGCTGCAGGAAGTAAGTTGCaa < 1:837780/71‑1 (MQ=255) ggCTGTGACGCATGCGGATCTTTAAAGCGAGAGATATCCGGGAAGTACAGCTGCAGGAAGTAAGTTGCaaa < 1:3197313/71‑1 (MQ=255) tgtgACGCATCCGGATCTTTAAAGCGAGAGATATCCGGGAAGTACAGCTGCAGGaa > 1:3547518/1‑56 (MQ=255) tgtgACGCATCCGGATCTTTAAAGCGAGAGATATCCGGGAAGTACAGCTGCAGGaa > 1:5084163/1‑56 (MQ=255) | GTAATGGTGGAGAAGATCAGCGCGCCAACCTGGAACGCTTTACCCGCAACGTACAGCATGATTTCAGGCTGTGACGCATCCGGATCTTTAAAGCGAGAGATATCCGGGAAGTACAGCTGCAGGAAGTAAGTTGCAAA > Exported/1306709‑1306845 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |