| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,309,899 | G→A | 85.3% | R31R (CGC→CGT) | yefM ← | antitoxin of the YoeB‑YefM toxin‑antitoxin system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,309,899 | 0 | G | A | 85.3% | 238.1 / 29.3 | 102 | R31R (CGC→CGT) | yefM | antitoxin of the YoeB‑YefM toxin‑antitoxin system |
| Reads supporting (aligned to +/- strand): ref base G (11/4); new base A (35/52); total (46/56) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.40e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.85e-01 | |||||||||||
Only 100 of 102 total aligned reads displayed.
GATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCG > Exported/1309832‑1309966 | gATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACg < 1:307540/70‑1 (MQ=255)gATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACg < 1:375809/70‑1 (MQ=255) tAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAg > 1:4804009/1‑70 (MQ=255) gCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGtaat < 1:1260765/71‑1 (MQ=255) ccGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGtaat > 1:563814/1‑70 (MQ=255) tctcTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAgg < 1:5079276/71‑1 (MQ=255) tctcTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAgg < 1:1687112/71‑1 (MQ=255) tctcTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAgg < 1:1680509/71‑1 (MQ=255) tctTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGAt > 1:4837448/1‑71 (MQ=255) tctTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGAt > 1:3974401/1‑71 (MQ=255) ttCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCg > 1:2110281/1‑71 (MQ=255) ttCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCg > 1:2767645/1‑71 (MQ=255) tCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGtaataa > 1:3595364/1‑64 (MQ=255) ccAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATc < 1:5694824/68‑1 (MQ=255) ccAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCggg > 1:1472472/1‑71 (MQ=255) gCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCa > 1:4670080/1‑71 (MQ=255) gCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCa > 1:2320264/1‑71 (MQ=255) gAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATg < 1:5058292/71‑1 (MQ=255) gAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATg < 1:5483549/71‑1 (MQ=255) gAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATg < 1:2856795/71‑1 (MQ=255) gAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATg < 1:924490/71‑1 (MQ=255) gAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATg < 1:1855555/71‑1 (MQ=255) aGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATg < 1:2404567/70‑1 (MQ=255) aGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGa < 1:596181/71‑1 (MQ=255) aGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGa < 1:623562/71‑1 (MQ=255) aGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGa < 1:5242047/71‑1 (MQ=255) ttGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATg < 1:4772241/68‑1 (MQ=255) ttGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATc < 1:4848120/71‑1 (MQ=255) gTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATg < 1:4951509/66‑1 (MQ=255) tATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTc < 1:1860552/71‑1 (MQ=255) tATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGtaat < 1:814493/50‑1 (MQ=255) tATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTc < 1:4752529/71‑1 (MQ=255) tATTCTTCGAGTGACATCAGAACACAAGCCTCTCCAATCTGACGAGtaat < 1:4781604/50‑1 (MQ=255) ttcttcGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGtaataa > 1:1152208/1‑50 (MQ=255) ttcttcGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGtaataa > 1:2477824/1‑50 (MQ=255) ttcttcGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCa < 1:3645021/70‑1 (MQ=255) cttcGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGa > 1:4092460/1‑41 (MQ=255) cttcGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGtaa > 1:1830025/1‑45 (MQ=255) cttcGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGtaa > 1:5476216/1‑45 (MQ=255) cttcGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAgg < 1:4494065/50‑1 (MQ=255) ttcGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACgg > 1:4045514/1‑71 (MQ=255) tcGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGAt > 1:2885872/1‑61 (MQ=255) cGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACgg < 1:2089928/69‑1 (MQ=255) cGAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCt > 1:4628665/1‑71 (MQ=255) gAGTGACATCAGTACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCtt < 1:2685810/71‑1 (MQ=255) gAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGa > 1:2381568/1‑37 (MQ=255) gAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCt < 1:1459859/70‑1 (MQ=255) gAGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCtt < 1:181699/71‑1 (MQ=255) aGTGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCtt < 1:5005876/70‑1 (MQ=255) tGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTtca > 1:2129596/1‑71 (MQ=255) tGACATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTtca > 1:2811616/1‑71 (MQ=255) aCATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCaa > 1:5301807/1‑60 (MQ=255) aCATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTtcatc < 1:1367165/71‑1 (MQ=255) cATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTtcatca < 1:3298004/71‑1 (MQ=255) cATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTtcatca < 1:1033985/71‑1 (MQ=255) cATCAGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTtcatca < 1:4921678/71‑1 (MQ=255) tCAGAACACAAGCCTCTCCATTCTGACGAGtaataa < 1:2123552/36‑1 (MQ=255) tCAGAACACAAGCCTCTCCATTCTGACGAGtaataa < 1:1969893/36‑1 (MQ=255) tCAGAACACAAGCCTCTCCATTCTGACGAGtaataa < 1:419525/36‑1 (MQ=255) aGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCAttg > 1:2838163/1‑70 (MQ=255) aGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCAttgt < 1:2397705/71‑1 (MQ=255) aGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCAttgt > 1:2054015/1‑71 (MQ=255) aGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCAttgt < 1:4439300/71‑1 (MQ=255) aGAACACAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCAttg < 1:2432562/70‑1 (MQ=255) aaCACAAGCCTCTCCATTCTGGCGAGTAATAAGGAt > 1:1414227/1‑36 (MQ=255) aaCACAAGCCTCTCCATTCTGGCGAGTAATAAGGAt > 1:83777/1‑36 (MQ=255) aaCACAAGCCTCTCCATTCTGGCGAGTAATAAGGATc < 1:1698133/37‑1 (MQ=255) acacAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTc < 1:3132828/50‑1 (MQ=255) acacAAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTc < 1:2220042/50‑1 (MQ=255) cacaAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGcc < 1:1063287/71‑1 (MQ=255) acaAGCCTCTCCATTCTGACGAGTAATAAGGATCGTGGCATg < 1:5485682/42‑1 (MQ=38) acaAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATg < 1:434549/42‑1 (MQ=255) acaAGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCg < 1:4834464/71‑1 (MQ=255) aaGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGAt > 1:4360644/1‑42 (MQ=255) aaGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGAt > 1:5387921/1‑42 (MQ=255) aaGCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGAt > 1:2088467/1‑42 (MQ=255) gCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGc > 1:1004216/1‑65 (MQ=255) gCCTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACaa < 1:2623447/71‑1 (MQ=255) gCCTCTCAATTCTGACGAGTAATAAGGATCGCGGCATGATCTTCAACgg > 1:4853078/1‑49 (MQ=38) ccTCTCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACaaa < 1:1830433/71‑1 (MQ=255) ctctCCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAAt < 1:2840273/71‑1 (MQ=255) ctcCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACaaa < 1:3797492/68‑1 (MQ=255) ctcCATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTc > 1:1588049/1‑71 (MQ=255) ccATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAAtt > 1:2867599/1‑68 (MQ=255) ccATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCa > 1:4460489/1‑39 (MQ=255) ccATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACgg > 1:2691628/1‑43 (MQ=255) ccATTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCt > 1:3642601/1‑70 (MQ=255) aTTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTcatcat < 1:3650908/51‑1 (MQ=255) aTTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTcatcat < 1:2476715/51‑1 (MQ=255) aTTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTcatcat < 1:1942955/51‑1 (MQ=255) aTTCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTcatcat < 1:5128160/51‑1 (MQ=255) tCTGACGAGTAATAAGGATCGGGGCATGATCTTCaa > 1:117807/1‑36 (MQ=255) tCTGACGAGTAATAAGGATCGGGGCATGATCTTCaa > 1:124621/1‑36 (MQ=255) tCTGACGAGTAATAAGGATCGGGGCATGATCTTCaa > 1:1937235/1‑36 (MQ=255) tCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCAttg > 1:3225048/1‑51 (MQ=255) tCTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAAt > 1:9081/1‑63 (MQ=255) cTGGCGAGTAATAAGGATCGGGGCATGATCTTCaa > 1:4725066/1‑35 (MQ=255) cTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAAtt > 1:1286488/1‑63 (MQ=255) cTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGAcgcg > 1:2561546/1‑71 (MQ=255) cTGACGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGAcgcg > 1:2743528/1‑71 (MQ=255) | GATAAGCCGTCTCTTCCAGCGAGTTGTATTCTTCGAGTGACATCAGAACACAAGCCTCTCCATTCTGGCGAGTAATAAGGATCGGGGCATGATCTTCAACGGCTTTCATCATTGTTGCCGACAAATTCTGACGCG > Exported/1309832‑1309966 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |