| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,314,255 | T→C | 78.3% | H170H (CAT→CAC) | hisB → | fused histidinol‑phosphatase and imidazoleglycerol‑phosphate dehydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,314,255 | 0 | T | C | 78.3% | 258.4 / 64.2 | 134 | H170H (CAT→CAC) | hisB | fused histidinol‑phosphatase and imidazoleglycerol‑phosphate dehydratase |
| Reads supporting (aligned to +/- strand): ref base T (12/17); new base C (47/57); total (60/74) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.33e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
Only 100 of 134 total aligned reads displayed.
CTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGC > Exported/1314186‑1314321 | cTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATg < 1:1035984/71‑1 (MQ=255)cTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATg > 1:5284348/1‑71 (MQ=255)cTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATg > 1:5290793/1‑71 (MQ=255) cGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGt < 1:3019115/69‑1 (MQ=255) aCCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTg > 1:5393828/1‑71 (MQ=255) ccGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGc < 1:5486157/71‑1 (MQ=255) gcgAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGt < 1:3418228/71‑1 (MQ=255) gcgAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGt < 1:1436222/71‑1 (MQ=255) cgAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTa < 1:4921825/71‑1 (MQ=255) gAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTaa < 1:697410/71‑1 (MQ=255) gAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTaa < 1:161905/71‑1 (MQ=255) gAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTa < 1:4463972/70‑1 (MQ=255) aaaCCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTaa > 1:4019501/1‑70 (MQ=255) aaaCCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACCCTCACGTAGTGCGTaa > 1:4887259/1‑70 (MQ=255) ccTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTTACCGTTACGCTCACGTAGTGCGTAATACCa < 1:1049798/71‑1 (MQ=255) aaCTGGCCAATGATTGGCGAGCAACTCACGAGACGTGACCGTTACGCTCATGTAGTGCGTaa > 1:1794010/1‑62 (MQ=255) aaCTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCaaa < 1:5418528/69‑1 (MQ=255) aaCTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAaga < 1:5573430/71‑1 (MQ=255) aaCTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAaga < 1:5454323/71‑1 (MQ=255) aCTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCaaa < 1:3783031/68‑1 (MQ=255) gCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGAc < 1:4331655/69‑1 (MQ=255) cAATGATTGGCGAGCAACTCACCAGACGTTACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAg < 1:2425948/71‑1 (MQ=255) cAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAg < 1:452643/71‑1 (MQ=255) aaTGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGt < 1:3676277/52‑1 (MQ=255) aaTGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACg > 1:3845091/1‑67 (MQ=255) aTGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGAt > 1:5554369/1‑71 (MQ=255) tGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTaa > 1:4960015/1‑52 (MQ=255) tGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt < 1:3982568/71‑1 (MQ=255) tGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGAtt < 1:2719386/71‑1 (MQ=255) tGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:2258133/1‑71 (MQ=255) tGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:4154477/1‑71 (MQ=255) tGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:4676729/1‑71 (MQ=255) tGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGAtt < 1:580656/71‑1 (MQ=255) gATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:1780201/1‑70 (MQ=255) gATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:1169088/1‑70 (MQ=255) gATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGAt > 1:3577223/1‑69 (MQ=255) gATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGAt > 1:3396886/1‑69 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTg < 1:3616828/70‑1 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTg < 1:5613962/70‑1 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTg < 1:1016402/70‑1 (MQ=255) ttGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCaa > 1:1744816/1‑55 (MQ=255) tGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGAAGCAGATTGACg > 1:522661/1‑71 (MQ=255) gCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGtt > 1:2442696/1‑71 (MQ=255) cGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTc < 1:2299890/71‑1 (MQ=255) gAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCa < 1:647680/71‑1 (MQ=255) gCAACTCACCAGACGTGACCGTTACGCTCAAGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAgg > 1:5271006/1‑71 (MQ=255) aCTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGtt > 1:4729005/1‑64 (MQ=255) aCTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTc > 1:636189/1‑65 (MQ=255) aCTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTc > 1:2513810/1‑65 (MQ=255) aCTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTc > 1:4582466/1‑65 (MQ=255) aCTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAgg < 1:1594189/68‑1 (MQ=255) aCTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAgg < 1:101533/68‑1 (MQ=255) aCTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAgg < 1:4472738/68‑1 (MQ=255) cTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAgg < 1:5612649/67‑1 (MQ=255) cTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAgg < 1:4182052/67‑1 (MQ=255) cTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAgg < 1:1945575/67‑1 (MQ=255) cTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGtgtg > 1:3233315/1‑71 (MQ=255) cTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGtgtg > 1:4406199/1‑71 (MQ=255) cTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGtgtg > 1:3240178/1‑71 (MQ=255) tCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTgg > 1:3614551/1‑71 (MQ=255) cACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTg > 1:2138166/1‑56 (MQ=255) cACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCa > 1:1065203/1‑63 (MQ=255) cACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCa > 1:2544391/1‑63 (MQ=255) cACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGc > 1:4318026/1‑71 (MQ=255) cACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGc > 1:1401442/1‑71 (MQ=255) cACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGc > 1:4563584/1‑71 (MQ=255) cACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGc > 1:2172168/1‑71 (MQ=255) cACCAGACGTGACAGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGc > 1:2685996/1‑71 (MQ=255) cAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTg > 1:2133591/1‑70 (MQ=255) aGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAg > 1:1425381/1‑48 (MQ=255) aGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGa < 1:2535897/71‑1 (MQ=255) gACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAgaga > 1:3219539/1‑42 (MQ=255) aCGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCa < 1:1636999/57‑1 (MQ=255) cGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCg < 1:4420331/71‑1 (MQ=255) gTGACCGTTACGCTCATGTAGTGCGTAATACCAAaga < 1:1056390/37‑1 (MQ=255) tGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTg < 1:3385040/71‑1 (MQ=255) tGACCGTTACGCTCACGTAGTGAGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTg < 1:2456677/71‑1 (MQ=255) aCCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGaa < 1:4347094/71‑1 (MQ=255) aCCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGa < 1:413611/70‑1 (MQ=255) cGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAgg < 1:755584/71‑1 (MQ=255) cGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAgg < 1:4539499/71‑1 (MQ=255) cGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAgg > 1:495521/1‑71 (MQ=255) cGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAgg < 1:4255181/71‑1 (MQ=255) cGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAgg < 1:1151664/71‑1 (MQ=255) cGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAgg < 1:1551010/71‑1 (MQ=255) gTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAgg < 1:183651/51‑1 (MQ=255) gTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAgg < 1:3163709/51‑1 (MQ=255) gTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAgg < 1:1533976/51‑1 (MQ=255) ttACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGaa < 1:1202911/67‑1 (MQ=255) ttACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAgg < 1:4106278/50‑1 (MQ=255) aCGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAg > 1:3697066/1‑47 (MQ=255) aCGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGgtgt > 1:4201326/1‑51 (MQ=255) aCGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGgtgt > 1:4765381/1‑51 (MQ=255) aCGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGgtgt > 1:3174328/1‑51 (MQ=255) aCGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAg > 1:5050649/1‑66 (MQ=255) cGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAg > 1:38727/1‑46 (MQ=255) cGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTg < 1:4380693/62‑1 (MQ=255) cTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGaa > 1:550617/1‑62 (MQ=255) cTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGaa > 1:2711543/1‑62 (MQ=255) tCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCa > 1:4030393/1‑42 (MQ=255) | CTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGC > Exported/1314186‑1314321 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |