Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,327,146 C→T 14.1% intergenic (‑136/+140) wcaK ← / ← wzxC predicted pyruvyl transferase/colanic acid exporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,327,1460CT14.1% 138.1 / 11.7 57intergenic (‑136/+140)wcaK/wzxCpredicted pyruvyl transferase/colanic acid exporter
Reads supporting (aligned to +/- strand):  ref base C (20/28);  new base T (2/6);  total (22/35)
Fisher's exact test for biased strand distribution p-value = 4.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGCGACGCTGACGCGTCTTATCAGGCCTACAAGACCCGAGCACAGAACCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACAGGTC  >  Exported/1327077‑1327208
                                                                     |                                                              
aTGCGACGCTGACGCGTCTTATCAGGCCTACAAGACCCGAGCACAGAACCGTAGGACGGATAAGGCGTTCa                                                               >  1:199089/1‑71 (MQ=255)
     aCGCTGACGCGTCTTATCAGGCCTACAAGACCCGAGCACAGAACCGTAGGACGGATAAGGCGTTCAcgcc                                                           <  1:4415337/70‑1 (MQ=255)
       gCTGACGCGTCTTATCAGGCCTACAAGACCCGAGCACAGAACCGTAGGACGGATAAGGCGTTCACGCCGCa                                                        <  1:3302846/71‑1 (MQ=255)
           aCGCGTCTTATCAGGCCTACAAGACCCGAGCACAGAACCGTAGGACGGATAAGGCGTTCACGCCGCATc                                                      <  1:1118124/69‑1 (MQ=255)
           aCGCGTCTTATCAGGCCTACAAGACCCGAGCACAGAACCGTAGGACGGATAAGGCGTTCACGCCGCATCCg                                                    >  1:3713051/1‑71 (MQ=255)
            cgcgTCTTATCAGGCCTACAAGACCCGAGCACAGAACCGTAGGACGGATAAGGCGTTCACGCCGCATCCgg                                                   <  1:207690/71‑1 (MQ=255)
             gcgTCTTATCAGGCCTACAAGACCCGAGCACAGAACCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGc                                                  <  1:2292852/71‑1 (MQ=255)
               gTCTTATCAGGCCTACAAGACCCGAGCACAGAACCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCAt                                                >  1:3404179/1‑71 (MQ=255)
                tCTTATCAGCCCTACAAGACCCGAGCACAGAACCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCAtt                                               <  1:1924439/71‑1 (MQ=255)
                  ttATCAGGCCTACAAGACCCGAGCACAGAACCGTAGGACGGATAAGGCGTTCACGCCGCAt                                                       >  1:4148574/1‑61 (MQ=255)
                  ttATCAGGCCTACAAGACCCGAGCACAGAACCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCa                                                 >  1:1181219/1‑67 (MQ=255)
                  ttATCAGGCCTACAAGACCCGAGCACAGAACCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCAtt                                               >  1:2375478/1‑69 (MQ=255)
                     tCAGGCCTACAAGACCCGAGCACAGAACCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTg                                          >  1:4129314/1‑71 (MQ=255)
                      cAGGCCTACAAGACCCGAGCACAGAACCGTAGGACGGATAAGGCGTTCAcgcc                                                           >  1:2802787/1‑53 (MQ=255)
                          ccTACAAGACCCGAGCACAGAACCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGa                                     <  1:1270555/71‑1 (MQ=255)
                           cTACAAGACCCGAGCACAGAACCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGAt                                    >  1:2301945/1‑71 (MQ=255)
                            tACAAGACCCGAGCACAGAACCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCa                                             >  1:2859225/1‑61 (MQ=255)
                              cAAGACCCGAGCACAGAACCGTAGGACGGATAAGGCGTTCa                                                               >  1:4365777/1‑41 (MQ=255)
                              cAAGACCCGAGCACAGAACCGTAGGACGGATAAGGCGTTCa                                                               >  1:4496235/1‑41 (MQ=255)
                              cAAGACCCGAGCACAGAACCGTAGGACGGATAAGGCGTTCa                                                               >  1:3080715/1‑41 (MQ=255)
                               aaGACCCGAGCACAGAACCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGa                                <  1:23414/71‑1 (MQ=255)
                                 gACCCGAGCACAGAACCGTAGGACGGATGCGGCGTGAAcgcctt                                                         <  1:3899978/44‑3 (MQ=11)
                                    ccGAGCACAGAACCGTAGGACGGATAAGGCGTTCACGCCGCATCCgg                                                   <  1:3648665/47‑1 (MQ=255)
                                      gAGCACAGAACCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCa                                                 >  1:4311658/1‑47 (MQ=255)
                                         cacaGAACCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCAt                                                <  1:3433983/45‑1 (MQ=255)
                                          acaGAACCGTAGGACGGATAAGGCGTTCACGCCGCATCCgg                                                   <  1:1782674/41‑1 (MQ=255)
                                           caaaaCCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGt                     >  1:1589576/4‑70 (MQ=255)
                                           caGAACCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCAtt                                               <  1:4478341/44‑1 (MQ=255)
                                            aGAACCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCAtt                                               <  1:3749507/43‑1 (MQ=255)
                                            aGAACCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTc                                              <  1:2551577/44‑1 (MQ=255)
                                             gAACCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTc                                              <  1:1346503/43‑1 (MQ=255)
                                             gAACCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCtt                  <  1:3948198/71‑1 (MQ=255)
                                             accccGTAGGACGGATAAGGCGTTTACGCTGCATCCGGCAACCTATGCCTGATGCGACGCTGAAGCGTCtt                  <  1:347533/68‑1 (MQ=11)
                                             accccGTAGGACGGATAAGGCGTTTACGCTGCATCCGGCAACCTATGCCTGATGCGACGCTGAAGCGTCt                   >  1:895719/4‑70 (MQ=11)
                                               cccGTAGGACGTATAAGGCGTTTACGCTGCATCCGGCAACCTATGCCTGATGCGACGCTGAAGCGTCTTAt                <  1:2099847/70‑1 (MQ=11)
                                               cccGTAGGACGGATAAGGCGTTTACGCTGCATCCGGCAACCTATGCCTGATGCGACGCTGAAGCGTCTTa                 <  1:2000872/69‑1 (MQ=11)
                                               cccGTAGGACGGATAAGGCGTTTACGCTGCATCCGGCAACCTATGCCTGATGCGACGCTGAAGCGTCTTAt                <  1:2093025/70‑1 (MQ=11)
                                               aCCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTAt                <  1:3576398/71‑1 (MQ=255)
                                               aCCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTAt                <  1:4782157/71‑1 (MQ=255)
                                                ccGTAGGACGGATAAGGCGTTTACGCTGCATCCGGCAACCTATGCCTGATGCGACGCTGAAGCGTCTTATc               <  1:1220100/71‑1 (MQ=11)
                                                ccGTAGGACGGATAAGGCGTTTACGCTGCATCCGGCAACCTATGCCTGATGCGACGCTGAAGCGTCTTATc               <  1:1226928/71‑1 (MQ=11)
                                                 cGTAGTACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATc               <  1:1832529/70‑1 (MQ=255)
                                                 cGTAGGACGGATAAGGCGTTTACGCTGCATCCGGCAACCTATGCCTGATGCGACGCTGAAGCGTCTTATCa              >  1:3286212/1‑71 (MQ=11)
                                                 cGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCa              <  1:2638605/71‑1 (MQ=255)
                                                 cGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCa              <  1:5316478/71‑1 (MQ=255)
                                                  gTAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGc                                         >  1:1305785/1‑43 (MQ=38)
                                                  ccaGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAg             >  1:5340866/3‑71 (MQ=9)
                                                   tAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCAgg            >  1:4931444/1‑71 (MQ=255)
                                                   tAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCAg             <  1:3563313/70‑1 (MQ=255)
                                                   caGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCt                            <  1:4084969/54‑1 (MQ=2)
                                                   caGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAg             <  1:1941816/69‑1 (MQ=14)
                                                   caGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAg             <  1:950276/69‑1 (MQ=14)
                                                     ggACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCAGGc           <  1:3405046/70‑1 (MQ=255)
                                                       aCGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGAcgc                       <  1:5170376/56‑1 (MQ=9)
                                                             aaGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACAGGTc  >  1:3858079/1‑71 (MQ=255)
                                                             aaGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACAGGTc  >  1:3192198/1‑71 (MQ=255)
                                                                   ttCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCa              <  1:5477899/53‑1 (MQ=255)
                                                                     cACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCAg             >  1:3742831/1‑52 (MQ=255)
                                                                     |                                                              
ATGCGACGCTGACGCGTCTTATCAGGCCTACAAGACCCGAGCACAGAACCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACAGGTC  >  Exported/1327077‑1327208

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: