Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,327,163 T→C 30.9% intergenic (‑153/+123) wcaK ← / ← wzxC predicted pyruvyl transferase/colanic acid exporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,327,1630TC30.9% 71.8 / 28.4 42intergenic (‑153/+123)wcaK/wzxCpredicted pyruvyl transferase/colanic acid exporter
Reads supporting (aligned to +/- strand):  ref base T (11/18);  new base C (3/10);  total (14/28)
Fisher's exact test for biased strand distribution p-value = 4.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

TCTTATCAGGCCTACAAGACCCGAGCACAGAACCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACAGGTCCCGAGCACAGAACCGTAG  >  Exported/1327093‑1327226
                                                                      |                                                               
tCTTATCAGCCCTACAAGACCCGAGCACAGAACCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCAtt                                                                 <  1:1924439/71‑1 (MQ=255)
  ttATCAGGCCTACAAGACCCGAGCACAGAACCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCAtt                                                                 >  1:2375478/1‑69 (MQ=255)
     tCAGGCCTACAAGACCCGAGCACAGAACCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTg                                                            >  1:4129314/1‑71 (MQ=255)
          ccTACAAGACCCGAGCACAGAACCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGa                                                       <  1:1270555/71‑1 (MQ=255)
           cTACAAGACCCGAGCACAGAACCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGAt                                                      >  1:2301945/1‑71 (MQ=255)
            tACAAGACCCGAGCACAGAACCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCa                                                               >  1:2859225/1‑61 (MQ=255)
               aaGACCCGAGCACAGAACCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGa                                                  <  1:23414/71‑1 (MQ=255)
                           caaaaCCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGt                                       >  1:1589576/4‑70 (MQ=255)
                           caGAACCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCAtt                                                                 <  1:4478341/44‑1 (MQ=255)
                            aGAACCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCAtt                                                                 <  1:3749507/43‑1 (MQ=255)
                            aGAACCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTc                                                                <  1:2551577/44‑1 (MQ=255)
                             gAACCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTc                                                                <  1:1346503/43‑1 (MQ=255)
                             gAACCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCtt                                    <  1:3948198/71‑1 (MQ=255)
                             accccGTAGGACGGATAAGGCGTTTACGCTGCATCCGGCAACCTATGCCTGATGCGACGCTGAAGCGTCtt                                    <  1:347533/68‑1 (MQ=11)
                             accccGTAGGACGGATAAGGCGTTTACGCTGCATCCGGCAACCTATGCCTGATGCGACGCTGAAGCGTCt                                     >  1:895719/4‑70 (MQ=11)
                               cccGTAGGACGTATAAGGCGTTTACGCTGCATCCGGCAACCTATGCCTGATGCGACGCTGAAGCGTCTTAt                                  <  1:2099847/70‑1 (MQ=11)
                               cccGTAGGACGGATAAGGCGTTTACGCTGCATCCGGCAACCTATGCCTGATGCGACGCTGAAGCGTCTTa                                   <  1:2000872/69‑1 (MQ=11)
                               cccGTAGGACGGATAAGGCGTTTACGCTGCATCCGGCAACCTATGCCTGATGCGACGCTGAAGCGTCTTAt                                  <  1:2093025/70‑1 (MQ=11)
                               aCCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTAt                                  <  1:4782157/71‑1 (MQ=255)
                               aCCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTAt                                  <  1:3576398/71‑1 (MQ=255)
                                ccGTAGGACGGATAAGGCGTTTACGCTGCATCCGGCAACCTATGCCTGATGCGACGCTGAAGCGTCTTATc                                 <  1:1226928/71‑1 (MQ=11)
                                ccGTAGGACGGATAAGGCGTTTACGCTGCATCCGGCAACCTATGCCTGATGCGACGCTGAAGCGTCTTATc                                 <  1:1220100/71‑1 (MQ=11)
                                 cGTAGTACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATc                                 <  1:1832529/70‑1 (MQ=255)
                                 cGTAGGACGGATAAGGCGTTTACGCTGCATCCGGCAACCTATGCCTGATGCGACGCTGAAGCGTCTTATCa                                >  1:3286212/1‑71 (MQ=11)
                                 cGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCa                                <  1:5316478/71‑1 (MQ=255)
                                 cGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCa                                <  1:2638605/71‑1 (MQ=255)
                                  gTAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGc                                                           >  1:1305785/1‑43 (MQ=38)
                                  ccaGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAg                               >  1:5340866/3‑71 (MQ=9)
                                   tAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCAgg                              >  1:4931444/1‑71 (MQ=255)
                                   tAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCAg                               <  1:3563313/70‑1 (MQ=255)
                                   caGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCt                                              <  1:4084969/54‑1 (MQ=2)
                                   caGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAg                               <  1:950276/69‑1 (MQ=14)
                                   caGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAg                               <  1:1941816/69‑1 (MQ=14)
                                     ggACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCAGGc                             <  1:3405046/70‑1 (MQ=255)
                                       aCGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGAcgc                                         <  1:5170376/56‑1 (MQ=9)
                                             aaGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACAGGTc                    >  1:3858079/1‑71 (MQ=255)
                                             aaGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACAGGTc                    >  1:3192198/1‑71 (MQ=255)
                                                   ttCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCa                                <  1:5477899/53‑1 (MQ=255)
                                                     cACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCAg                               >  1:3742831/1‑52 (MQ=255)
                                                          cgcATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACAGGTccc                  <  1:2828115/60‑1 (MQ=255)
                                                          cgcATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACAGGTCCCGAGCACAGAAc       <  1:3956110/71‑1 (MQ=255)
                                                            cATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACAGGTCCCGAGCACAGAACCg     <  1:4400215/71‑1 (MQ=255)
                                                              tCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATc                                 <  1:4169605/41‑1 (MQ=255)
                                                              tCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATc                                 <  1:478267/41‑1 (MQ=255)
                                                                cGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACAGGTCCCGAGCACAGaa        >  1:1200941/1‑64 (MQ=255)
                                                                cGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACAGGTCCCGAGCACAGAACCGTAg  >  1:2889671/1‑70 (MQ=255)
                                                                     ttCAGTGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAGGTCCCGAGCACAGAACCGTAg  <  1:1815605/65‑1 (MQ=255)
                                                                     ttCAGTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACAGGTCCCGAGca             <  1:1959955/54‑1 (MQ=255)
                                                                      |                                                               
TCTTATCAGGCCTACAAGACCCGAGCACAGAACCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACAGGTCCCGAGCACAGAACCGTAG  >  Exported/1327093‑1327226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: