Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | Exported | 1,327,185 | C→A | 15.0% | intergenic (‑175/+101) | wcaK ← / ← wzxC | predicted pyruvyl transferase/colanic acid exporter |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | Exported | 1,327,185 | 0 | C | A | 15.0% | 144.5 / 10.0 | 53 | intergenic (‑175/+101) | wcaK/wzxC | predicted pyruvyl transferase/colanic acid exporter |
Reads supporting (aligned to +/- strand): ref base C (14/31); new base A (2/6); total (16/37) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CAGAACCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACAGGTCCCGAGCACAGAACCGTAGGACGGATAAGGCGTTTTACGCCGCATCCG > Exported/1327120‑1327255 | caaaaCCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGt > 1:1589576/4‑70 (MQ=255) gAACCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCtt < 1:3948198/71‑1 (MQ=255) accccGTAGGACGGATAAGGCGTTTACGCTGCATCCGGCAACCTATGCCTGATGCGACGCTGAAGCGTCtt < 1:347533/68‑1 (MQ=11) accccGTAGGACGGATAAGGCGTTTACGCTGCATCCGGCAACCTATGCCTGATGCGACGCTGAAGCGTCt > 1:895719/4‑70 (MQ=11) cccGTAGGACGTATAAGGCGTTTACGCTGCATCCGGCAACCTATGCCTGATGCGACGCTGAAGCGTCTTAt < 1:2099847/70‑1 (MQ=11) cccGTAGGACGGATAAGGCGTTTACGCTGCATCCGGCAACCTATGCCTGATGCGACGCTGAAGCGTCTTa < 1:2000872/69‑1 (MQ=11) cccGTAGGACGGATAAGGCGTTTACGCTGCATCCGGCAACCTATGCCTGATGCGACGCTGAAGCGTCTTAt < 1:2093025/70‑1 (MQ=11) aCCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTAt < 1:3576398/71‑1 (MQ=255) aCCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTAt < 1:4782157/71‑1 (MQ=255) ccGTAGGACGGATAAGGCGTTTACGCTGCATCCGGCAACCTATGCCTGATGCGACGCTGAAGCGTCTTATc < 1:1220100/71‑1 (MQ=11) ccGTAGGACGGATAAGGCGTTTACGCTGCATCCGGCAACCTATGCCTGATGCGACGCTGAAGCGTCTTATc < 1:1226928/71‑1 (MQ=11) cGTAGTACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATc < 1:1832529/70‑1 (MQ=255) cGTAGGACGGATAAGGCGTTTACGCTGCATCCGGCAACCTATGCCTGATGCGACGCTGAAGCGTCTTATCa > 1:3286212/1‑71 (MQ=11) cGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCa < 1:5316478/71‑1 (MQ=255) cGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCa < 1:2638605/71‑1 (MQ=255) ccaGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAg > 1:5340866/3‑71 (MQ=9) tAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCAgg > 1:4931444/1‑71 (MQ=255) tAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCAg < 1:3563313/70‑1 (MQ=255) caGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAg < 1:950276/69‑1 (MQ=14) caGGACGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGACGCGTCTTATCAg < 1:1941816/69‑1 (MQ=14) ggACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCAGGc < 1:3405046/70‑1 (MQ=255) aCGGATAAGGCTTTCACGCCTTATCCGACAACAACTGCCTGATGCGACGCTGAcgc < 1:5170376/56‑1 (MQ=9) aaGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACAGGTc > 1:3192198/1‑71 (MQ=255) aaGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACAGGTc > 1:3858079/1‑71 (MQ=255) ttCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCa < 1:5477899/53‑1 (MQ=255) cACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCAg > 1:3742831/1‑52 (MQ=255) cgcATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACAGGTccc < 1:2828115/60‑1 (MQ=255) cgcATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACAGGTCCCGAGCACAGAAc < 1:3956110/71‑1 (MQ=255) cATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACAGGTCCCGAGCACAGAACCg < 1:4400215/71‑1 (MQ=255) tCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATc < 1:478267/41‑1 (MQ=255) tCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATc < 1:4169605/41‑1 (MQ=255) cGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACAGGTCCCGAGCACAGaa > 1:1200941/1‑64 (MQ=255) cGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACAGGTCCCGAGCACAGAACCGTAg > 1:2889671/1‑70 (MQ=255) ttCAGTGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAGGTCCCGAGCACAGAACCGTAg < 1:1815605/65‑1 (MQ=255) ttCAGTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACAGGTCCCGAGca < 1:1959955/54‑1 (MQ=255) cAGTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACAGGTCCCGAGCACAGAACCGTAGGACGGAt < 1:608985/70‑1 (MQ=255) tGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACAGGTCCCGAGCACAGAACCGTAGGACGGATAAgg > 1:1385006/1‑71 (MQ=39) ccTGATGCGACGCTGACGCGTCTTATCAGGCCTACAGGTCCCGAGCACAGAACCGTAGGACGGATAAGGCg < 1:3449394/71‑1 (MQ=39) gATGCGACGCTGACGCGTCTTATCAGGCCTACAGGTCCCGAGCACAGAACCGTAGGACGGATAAGGCGttt > 1:883617/1‑71 (MQ=40) aTGCGACGCTGACGCGTCTTATCAGGCCTACAGGTCCCGAGCACAGAACCGTAGGACGGATAAGGCGttt < 1:5063851/70‑1 (MQ=255) tGCGACGCTGACGCGTCTTATCAGGCCTACAGGTccc < 1:2329526/37‑1 (MQ=255) cGACGCTGACGCGTCTTATCAGGCCTACAGGTCCCGAGCACAGAACCGTAGGACGGATAAGGCGTTTTAcg > 1:2645926/1‑71 (MQ=255) cGACGCTGACGCGTCTTATCAGGCCTACAGGTCCCGAGCACAGAACCGTAGGACGGATAAGGCGTTTTAc < 1:4167895/70‑1 (MQ=255) cGACGCTGACGCGTCTTATCAGGCCTACAGGTCCCGAGCACAGAACCGTAGGACGGATAAGGCGTTTTAc > 1:1559463/1‑70 (MQ=255) cGCTGACGCGTCTTATCAGGCCTACAGGTCCCGAGCACAGAACCGTAGGa < 1:1834838/50‑1 (MQ=255) cGCTGACGCGTCTTATCAGGCCTACAGGTCCCGAGCACAGAACCGTAGGa < 1:5540024/50‑1 (MQ=255) gCTGACGCGTCTTATCAGGCCTACAGGTCCCGAGCACAGAACCGTAGGACg < 1:2689230/51‑1 (MQ=255) gCTGACGCGTCTTATCAGGCCTACAGGTCCCGAGCACAGAACCGTAGGACg < 1:4302795/51‑1 (MQ=255) gCTGACGCGTCTTATCAGGCCTACAGGTCCCGAGCACAGAACCGTAGGACGGATAAGGCGTTTTAcgccg > 1:3448418/1‑70 (MQ=255) cTGACGCGTCTTATCAGGCCTACAGGTCCCGAGCACAGAACCGTAGGAc < 1:421379/49‑1 (MQ=255) cTGACGCGTCTTATCAGGCCTACAGGTCCCGAGCACAGAACCGTAGGAc < 1:2202897/49‑1 (MQ=255) cTGACGCGTCTTATCAGGCCTACAGGTCCCGAGCACAGAACCGTAGGAc < 1:2338759/49‑1 (MQ=255) gACGCGTCTTATCAGGCCTAAAGGTCCCGAGCACAGAAc > 1:5619618/1‑39 (MQ=255) aCGCGTCTTATCAGGCCTACAGGTCCCGAGCACAGAACCGTAGGACGGATAAGGCGTTTTACGCCGCATc < 1:1860875/70‑1 (MQ=255) cgcgTCTTATCAGGCCTACAGGTCCCGAGCACAGAACCGTAGGACGGATAAGGCGTTTTACGCCGCATCCg < 1:592782/71‑1 (MQ=255) | CAGAACCGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCATTCAGTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACAGGTCCCGAGCACAGAACCGTAGGACGGATAAGGCGTTTTACGCCGCATCCG > Exported/1327120‑1327255 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |