| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,395,375 | C→G | 17.4% | T27T (ACC→ACG) | setB → | lactose/glucose efflux system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,395,375 | 0 | C | G | 17.4% | 158.2 / 25.3 | 69 | T27T (ACC→ACG) | setB | lactose/glucose efflux system |
| Reads supporting (aligned to +/- strand): ref base C (35/22); new base G (3/9); total (38/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.78e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01 | |||||||||||
CCCGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTC > Exported/1395307‑1395442 | cccGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCgg < 1:2579060/71‑1 (MQ=255) ccGCAGTCTCCAGCGCGAAATCGTATGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCgg > 1:635835/1‑70 (MQ=255) cGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTa > 1:5225024/1‑71 (MQ=255) gCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTAt > 1:660005/1‑71 (MQ=255) gCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTAt > 1:5306107/1‑71 (MQ=255) gCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGGTTTTAATCGTTGCCTTTCTCACCGGTAt > 1:1389234/1‑71 (MQ=255) ctcCAGCGCGAAATCGTTTGACCGGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCgg > 1:3612387/1‑71 (MQ=255) tcCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCg < 1:968773/61‑1 (MQ=255) tcCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCggg > 1:4405991/1‑71 (MQ=255) cAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGgcg > 1:4176297/1‑71 (MQ=255) cAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGgcg > 1:729124/1‑71 (MQ=255) cgAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTg > 1:1719305/1‑71 (MQ=255) cgAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTg > 1:7046/1‑71 (MQ=255) cgAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTg > 1:1707370/1‑71 (MQ=255) gAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGt < 1:4810592/55‑1 (MQ=255) gAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGt < 1:2350916/55‑1 (MQ=255) aaaTCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCAcc < 1:1375379/51‑1 (MQ=255) aaaTCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCAcc < 1:2395392/51‑1 (MQ=255) aaaTCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCAcc < 1:1220346/51‑1 (MQ=255) aaaTCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGt < 1:3022663/54‑1 (MQ=255) aaTCGTTTGACCTGACCTCGAGGGCGTTTTTAATCGTTGCCTTTCTCACCGGTAt > 1:5423496/1‑55 (MQ=255) aaTCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTAt > 1:1540246/1‑55 (MQ=255) aTCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCgg < 1:2479554/51‑1 (MQ=255) aTCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCgg < 1:902423/51‑1 (MQ=255) aTCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCgg < 1:1561261/51‑1 (MQ=255) gTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAAccc > 1:2179918/1‑71 (MQ=255) ttGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTa > 1:3335461/1‑48 (MQ=255) ttGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTa > 1:459019/1‑48 (MQ=255) ttGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTa > 1:4508323/1‑48 (MQ=255) ttGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAAcccc > 1:1240193/1‑70 (MQ=255) gacctCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGgcg < 1:3104764/50‑1 (MQ=255) tCGGCGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAAcccc < 1:5507853/59‑1 (MQ=255) tCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCaa < 1:3094563/54‑1 (MQ=255) ccgcGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCTCTCTTCAGACTCCTACCCTAAGTa < 1:1912440/66‑1 (MQ=255) ccgcGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTa < 1:961379/66‑1 (MQ=255) ccgcGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTa < 1:5461045/66‑1 (MQ=255) ccgcGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTa < 1:5606809/66‑1 (MQ=255) cGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGgcg > 1:4075428/1‑45 (MQ=255) cGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAAccc > 1:570409/1‑57 (MQ=255) gACGGCGTTTATAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTAttt > 1:345318/1‑71 (MQ=255) cGGCATTTTTACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTAtattc < 1:1329297/71‑2 (MQ=255) gcatttttACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCt < 1:5433845/68‑1 (MQ=255) gcatttttACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCt < 1:433957/68‑1 (MQ=255) gTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTa > 1:3885576/1‑71 (MQ=255) atttttACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCtcg > 1:3109744/2‑69 (MQ=255) tttttACTTGTCGCCTTTCTGACGGGTATTGCGGgcg < 1:1611621/37‑1 (MQ=12) tttttACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCa < 1:4844851/44‑1 (MQ=16) tttttAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGa < 1:4913978/52‑1 (MQ=255) ttttAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTAcc > 1:5049856/1‑71 (MQ=255) ttttAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTAcc > 1:2495962/1‑71 (MQ=255) tttACTTGTCGCCTTTCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCtaa > 1:3363584/1‑54 (MQ=255) tttAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTg > 1:4477010/1‑40 (MQ=255) tttAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTAcc > 1:4926033/1‑70 (MQ=255) ttAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGa > 1:4581485/1‑71 (MQ=255) aTCGTTGCCTTTCTCACCGGTATTGCGGGTGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATg < 1:2188262/70‑1 (MQ=255) aTCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGa < 1:4649244/71‑1 (MQ=255) tCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAAcccc < 1:4514188/43‑1 (MQ=255) tCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAAcccc < 1:5166761/43‑1 (MQ=255) tCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCtt < 1:5064535/62‑1 (MQ=255) ttGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTa < 1:5577743/71‑1 (MQ=255) ttGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTa < 1:3752649/71‑1 (MQ=255) gCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGt > 1:5173362/1‑68 (MQ=255) ccTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACAt < 1:3398826/71‑1 (MQ=255) ttCTGACGGGTATTGCGGGCGCTCTTCAGACTCCTACCCTAAGTATATTCCTCGCAGATGaa > 1:1215099/1‑62 (MQ=255) ttCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGcc > 1:4008181/1‑71 (MQ=255) ttCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGcc > 1:796732/1‑71 (MQ=255) tctcACCGGTATTGCGGGCGCTCTGCAAACCCCGac > 1:1206445/1‑36 (MQ=255) tctcACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTGTTACCGATGAAGTACATGccc > 1:2692158/1‑71 (MQ=255) tctcACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGccc > 1:1987298/1‑71 (MQ=255) ctcACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGccc > 1:557475/1‑70 (MQ=255) aCCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTa < 1:2298029/40‑1 (MQ=255) aCCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTc < 1:4786186/70‑1 (MQ=255) | CCCGCAGTCTCCAGCGCGAAATCGTTTGACCTGACCTCGACGGCGTTTTTAATCGTTGCCTTTCTCACCGGTATTGCGGGCGCTCTGCAAACCCCGACACTCAGTATTTTTCTTACCGATGAAGTACATGCCCGTC > Exported/1395307‑1395442 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |