Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,395,705 C→A 15.7% T137T (ACC→ACA setB → lactose/glucose efflux system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,395,7050CA15.7% 319.2 / 29.4 114T137T (ACC→ACAsetBlactose/glucose efflux system
Reads supporting (aligned to +/- strand):  ref base C (46/49);  new base A (10/8);  total (56/58)
Fisher's exact test for biased strand distribution p-value = 6.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 115 total aligned reads displayed.

TCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCC  >  Exported/1395636‑1395772
                                                                     |                                                                   
     gcagcTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGt                                                               <  1:2887441/71‑1 (MQ=255)
     gcagcTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACAGGACGt                                                               <  1:5205782/71‑1 (MQ=255)
      cagcTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATTCCGACAAAACCGGACGTg                                                              <  1:3663542/71‑1 (MQ=255)
      cagcTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTg                                                              <  1:1868552/71‑1 (MQ=255)
             ggCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCgg                                                        <  1:2464191/70‑1 (MQ=255)
             ggCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCgg                                                        <  1:3938887/70‑1 (MQ=255)
              gCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTg                                                      <  1:2601181/71‑1 (MQ=255)
                tCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGAt                                                    >  1:4926446/1‑71 (MQ=255)
                tCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGAt                                                    >  1:1455267/1‑71 (MQ=255)
                 cGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTg                                                              >  1:3630451/1‑60 (MQ=255)
                 cGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATg                                                   >  1:3794624/1‑71 (MQ=255)
                   aCCGCAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGtt                                                 <  1:1748280/71‑1 (MQ=255)
                   aCCGCAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGtt                                                 <  1:1246216/71‑1 (MQ=255)
                   aCCGCAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGtt                                                 <  1:5499068/71‑1 (MQ=255)
                     cGCAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAggcc                                                                 >  1:3379320/1‑51 (MQ=255)
                     cGCAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGtt                                                 >  1:5679529/1‑69 (MQ=255)
                      gCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAg                                              >  1:2190858/1‑71 (MQ=255)
                       cTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGc                                             >  1:3744981/1‑71 (MQ=255)
                       cTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGc                                             >  1:449811/1‑71 (MQ=255)
                       cTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGc                                             <  1:1596494/71‑1 (MQ=255)
                         aaCCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGctc                                           >  1:4711307/1‑71 (MQ=255)
                          aCCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGctct                                          >  1:2786936/1‑71 (MQ=255)
                          aCCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACg                                                         >  1:3234405/1‑56 (MQ=255)
                          aCCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAgt                                             <  1:3406017/68‑2 (MQ=255)
                            ccGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCttt                                        <  1:4381194/71‑1 (MQ=255)
                            ccGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCttt                                        <  1:1777486/71‑1 (MQ=255)
                             cGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCtttt                                       <  1:5526964/71‑1 (MQ=255)
                             cGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCttt                                        <  1:4016472/70‑1 (MQ=255)
                              gCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTg                                                      <  1:4100766/55‑1 (MQ=255)
                              gCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGa                                                     >  1:2115148/1‑56 (MQ=255)
                              gCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCttttt                                      >  1:4897041/1‑71 (MQ=255)
                              gCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCttttt                                      <  1:2502730/71‑1 (MQ=255)
                              gCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCtt                                         <  1:1207736/68‑1 (MQ=255)
                              gCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCAt                                                    >  1:1305554/1‑57 (MQ=255)
                               cAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCtttttt                                     <  1:3541979/71‑1 (MQ=255)
                                aaaTGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACattttt                                     >  1:2876511/1‑70 (MQ=255)
                                aaaTGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTa                                    >  1:2025824/1‑71 (MQ=255)
                                 aaTGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCg                                                                    <  1:4948479/38‑1 (MQ=255)
                                 aaTGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTAc                                   >  1:170295/1‑71 (MQ=255)
                                   tGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCgg                                                                   >  1:3330730/1‑37 (MQ=255)
                                   tGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGAc                                                                 >  1:828424/1‑39 (MQ=255)
                                   tGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGAc                                                                 >  1:567505/1‑39 (MQ=255)
                                    gTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTAcg                                  >  1:4327109/1‑69 (MQ=255)
                                     tttGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCttttt                                      <  1:4347731/64‑1 (MQ=255)
                                     tttGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTa                                    <  1:661291/66‑1 (MQ=255)
                                      ttgcccttgcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCt                              >  1:576392/1‑71 (MQ=255)
                                       tgcccttgcccGTGAACATGCCGACAAAACCGGACGt                                                               >  1:5201057/1‑37 (MQ=255)
                                       tgcccttgcccGTGAACATGCCGACAAAACCGGACGt                                                               >  1:1917951/1‑37 (MQ=255)
                                       tgcccttgcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCttttt                                      >  1:686189/1‑62 (MQ=255)
                                       tgcccttgcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCtt                                         <  1:2221757/59‑1 (MQ=255)
                                       tgcccttgcccGTGAACATGACGACAAAACCGGACGt                                                               >  1:1833024/1‑37 (MQ=255)
                                        gcccttgcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCt                              >  1:5071142/1‑69 (MQ=255)
                                        gcccttgcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTc                             <  1:5692343/70‑1 (MQ=255)
                                        gcccttgcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTc                             <  1:3412457/70‑1 (MQ=255)
                                         cccttgcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCa                            <  1:1567975/70‑1 (MQ=255)
                                           cttgcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGt                         >  1:3063026/1‑71 (MQ=255)
                                           cttgcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGt                         >  1:444304/1‑71 (MQ=255)
                                            ttgcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGt                                                       <  1:4801565/40‑1 (MQ=255)
                                            ttgcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGt                                                       <  1:3018376/40‑1 (MQ=255)
                                            ttgcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCttttt                                      <  1:2090912/57‑1 (MQ=255)
                                            ttgcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAg                           <  1:1125024/68‑1 (MQ=255)
                                            tcgcccGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACattttt                                     <  1:4686250/56‑1 (MQ=255)
                                            tcgcccGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTa                                    >  1:839972/3‑59 (MQ=255)
                                            tcgcccGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTa                                    >  1:3694005/3‑59 (MQ=255)
                                             cgcccGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTAc                                   >  1:3942509/2‑59 (MQ=255)
                                              gcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGttt                       <  1:800569/70‑1 (MQ=255)
                                               cccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCa                     <  1:3983690/71‑1 (MQ=255)
                                                ccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCa                     <  1:3915962/70‑1 (MQ=255)
                                                ccGAGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGttt                       <  1:2147932/68‑1 (MQ=255)
                                                 cGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGttt                       <  1:2228594/67‑1 (MQ=255)
                                                  gTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCa                     >  1:1669390/1‑68 (MQ=255)
                                                  gTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTg                  >  1:1913207/1‑71 (MQ=255)
                                                  gTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTg                  <  1:5150326/71‑1 (MQ=255)
                                                    gAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCttt                                        <  1:2201812/47‑1 (MQ=255)
                                                     aaCATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCAc                    >  1:755256/1‑66 (MQ=255)
                                                     aaCATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCAc                    >  1:4962930/1‑66 (MQ=255)
                                                     aaCATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCa               >  1:413784/1‑71 (MQ=255)
                                                     aaCATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCa               >  1:1269982/1‑71 (MQ=255)
                                                     aaCATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCa               <  1:3217458/71‑1 (MQ=255)
                                                         tGCCGACAAAACCGGACGTGAGGAGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATggg           >  1:4571971/1‑71 (MQ=255)
                                                          gCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATggg           >  1:4085080/1‑70 (MQ=255)
                                                           ccGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGttt                       <  1:4090419/57‑1 (MQ=255)
                                                           ccGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGttt                       <  1:4097122/57‑1 (MQ=255)
                                                           ccGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGt          <  1:3891430/70‑1 (MQ=255)
                                                           ccGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTc         <  1:2122605/71‑1 (MQ=255)
                                                            cGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCt                              <  1:408682/49‑1 (MQ=255)
                                                             gACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCAt       <  1:3971058/71‑1 (MQ=255)
                                                              aCAAAACTGGACGTGAGGCGGTGATGTTCAGCTCtttt                                       <  1:1221231/38‑1 (MQ=255)
                                                              aCAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCa                     >  1:2366821/1‑56 (MQ=255)
                                                              aCAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCa                     >  1:3479667/1‑56 (MQ=255)
                                                              aCAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCa                     >  1:1879672/1‑56 (MQ=255)
                                                              aCAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCAtt      <  1:5426095/71‑1 (MQ=255)
                                                               cAAAACCGGACGTGAGGCGGTGATGTTCAGCTCttttt                                      <  1:4366530/38‑1 (MQ=255)
                                                               cAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCAc                    >  1:2882481/1‑56 (MQ=255)
                                                                aaaaCCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTgg    >  1:160445/1‑71 (MQ=255)
                                                                aaaaCCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTgg    >  1:4104137/1‑71 (MQ=255)
                                                                  aaCCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGcc  >  1:4932554/1‑71 (MQ=255)
                                                                   aCCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTAcg                                  >  1:5235159/1‑38 (MQ=255)
                                                                   aCCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTAcg                                  >  1:1032836/1‑38 (MQ=255)
                                                                     cGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCAt       >  1:2878861/1‑63 (MQ=255)
                                                                     |                                                                   
TCTTAGCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCC  >  Exported/1395636‑1395772

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: