Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,395,708 A→C 14.3% G138G (GGA→GGC setB → lactose/glucose efflux system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,395,7080AC14.3% 225.2 / 39.3 112G138G (GGA→GGCsetBlactose/glucose efflux system
Reads supporting (aligned to +/- strand):  ref base A (44/52);  new base C (9/7);  total (53/59)
Fisher's exact test for biased strand distribution p-value = 5.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.43e-01

Only 100 of 113 total aligned reads displayed.

GCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCG  >  Exported/1395641‑1395777
                                                                   |                                                                     
gcagcTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACAGGACGt                                                                    <  1:5205782/71‑1 (MQ=255)
 cagcTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATTCCGACAAAACCGGACGTg                                                                   <  1:3663542/71‑1 (MQ=255)
 cagcTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTg                                                                   <  1:1868552/71‑1 (MQ=255)
       tGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGc                                                               <  1:2859038/69‑1 (MQ=255)
       tGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGc                                                               <  1:4069707/69‑1 (MQ=255)
        ggCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCgg                                                             <  1:2464191/70‑1 (MQ=255)
        ggCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCgg                                                             <  1:655697/70‑1 (MQ=255)
        ggCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCgg                                                             <  1:3938887/70‑1 (MQ=255)
         gCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTg                                                           <  1:2601181/71‑1 (MQ=255)
          cTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACg                                                                     <  1:1969996/60‑1 (MQ=255)
           tCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGAt                                                         >  1:4926446/1‑71 (MQ=255)
            cGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTg                                                                   >  1:3630451/1‑60 (MQ=255)
            cGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATg                                                        >  1:3794624/1‑71 (MQ=255)
              aCCGCAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGtt                                                      <  1:5499068/71‑1 (MQ=255)
              aCCGCAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGtt                                                      <  1:1754783/71‑1 (MQ=255)
              aCCGCAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGtt                                                      <  1:1748280/71‑1 (MQ=255)
              aCCGCAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGtt                                                      <  1:1246216/71‑1 (MQ=255)
                cGCAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGtt                                                      >  1:5679529/1‑69 (MQ=255)
                cGCAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGtt                                                      >  1:4275324/1‑69 (MQ=255)
                 gCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAg                                                   >  1:2190858/1‑71 (MQ=255)
                  cTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGc                                                  <  1:1596494/71‑1 (MQ=255)
                  cTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGc                                                  >  1:449811/1‑71 (MQ=255)
                  cTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGc                                                  >  1:3744981/1‑71 (MQ=255)
                     aCCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGctct                                               >  1:2786936/1‑71 (MQ=255)
                     aCCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACg                                                              >  1:3234405/1‑56 (MQ=255)
                     aCCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAgt                                                  <  1:3406017/68‑2 (MQ=255)
                       ccGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCttt                                             <  1:4381194/71‑1 (MQ=255)
                        cGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCtttt                                            <  1:5526964/71‑1 (MQ=255)
                        cGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCttt                                             <  1:4016472/70‑1 (MQ=255)
                         gCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTg                                                           <  1:4100766/55‑1 (MQ=255)
                         gCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCttttt                                           >  1:4897041/1‑71 (MQ=255)
                         gCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCttttt                                           <  1:2502730/71‑1 (MQ=255)
                         gCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCtt                                              <  1:1207736/68‑1 (MQ=255)
                         gCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCAt                                                         >  1:1305554/1‑57 (MQ=255)
                          cAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCtttttt                                          <  1:3541979/71‑1 (MQ=255)
                           aaaTGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACattttt                                          >  1:2876511/1‑70 (MQ=255)
                           aaaTGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTa                                         >  1:2025824/1‑71 (MQ=255)
                            aaTGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTAc                                        >  1:170295/1‑71 (MQ=255)
                              tGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGa                                                                       >  1:2205589/1‑38 (MQ=255)
                              tGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGAc                                                                      >  1:828424/1‑39 (MQ=255)
                              tGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGAc                                                                      >  1:567505/1‑39 (MQ=255)
                               gTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTAcgcg                                     >  1:4945487/1‑71 (MQ=255)
                               gTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTAcgcg                                     >  1:3163144/1‑71 (MQ=255)
                               gTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTAcg                                       >  1:4327109/1‑69 (MQ=255)
                                tttGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTa                                         <  1:661291/66‑1 (MQ=255)
                                  tgcccttgcccGTGAACATGCCGACAAAACCGGACGt                                                                    >  1:1917951/1‑37 (MQ=255)
                                  tgcccttgcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCttttt                                           >  1:686189/1‑62 (MQ=255)
                                  tgcccttgcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCtt                                              <  1:2221757/59‑1 (MQ=255)
                                  tgcccttgcccGTGAACATGACGACAAAACCGGACGt                                                                    >  1:1833024/1‑37 (MQ=255)
                                   gcccttgcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCt                                   >  1:5071142/1‑69 (MQ=255)
                                   gcccttgcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTc                                  <  1:3412457/70‑1 (MQ=255)
                                   gcccttgcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTc                                  <  1:5692343/70‑1 (MQ=255)
                                    cccttgcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCa                                 <  1:1567975/70‑1 (MQ=255)
                                      cttgcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGt                              >  1:444304/1‑71 (MQ=255)
                                      cttgcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGt                              >  1:3063026/1‑71 (MQ=255)
                                       ttgcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGt                                                            <  1:4801565/40‑1 (MQ=255)
                                       ttgcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGt                                                            <  1:3018376/40‑1 (MQ=255)
                                       ttgcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCttttt                                           <  1:2090912/57‑1 (MQ=255)
                                       ttgcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAg                                <  1:1125024/68‑1 (MQ=255)
                                       tcgcccGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACattttt                                          <  1:4686250/56‑1 (MQ=255)
                                       tcgcccGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTa                                         >  1:839972/3‑59 (MQ=255)
                                       tcgcccGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTa                                         >  1:3694005/3‑59 (MQ=255)
                                        cgcccGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTAc                                        >  1:3942509/2‑59 (MQ=255)
                                         gcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGttt                            <  1:800569/70‑1 (MQ=255)
                                          cccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCa                          <  1:3983690/71‑1 (MQ=255)
                                           ccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCa                          <  1:3915962/70‑1 (MQ=255)
                                           ccGAGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGttt                            <  1:2147932/68‑1 (MQ=255)
                                            cGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGttt                            <  1:2228594/67‑1 (MQ=255)
                                             gTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCa                          >  1:1669390/1‑68 (MQ=255)
                                             gTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTg                       <  1:5150326/71‑1 (MQ=255)
                                             gTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTg                       >  1:1913207/1‑71 (MQ=255)
                                               gAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCttt                                             <  1:2201812/47‑1 (MQ=255)
                                                aaCATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCAc                         >  1:755256/1‑66 (MQ=255)
                                                aaCATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCAc                         >  1:4962930/1‑66 (MQ=255)
                                                aaCATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCa                    >  1:413784/1‑71 (MQ=255)
                                                aaCATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCa                    >  1:1269982/1‑71 (MQ=255)
                                                aaCATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCa                    <  1:3217458/71‑1 (MQ=255)
                                                    tGCCGACAAAACCGGACGTGAGGAGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATggg                >  1:4571971/1‑71 (MQ=255)
                                                      ccGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGttt                            <  1:4097122/57‑1 (MQ=255)
                                                      ccGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGttt                            <  1:4090419/57‑1 (MQ=255)
                                                      ccGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTc              <  1:2122605/71‑1 (MQ=255)
                                                       cGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCt                                   <  1:408682/49‑1 (MQ=255)
                                                        gACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCAt            <  1:3971058/71‑1 (MQ=255)
                                                         aCAAAACTGGACGTGAGGCGGTGATGTTCAGCTCtttt                                            <  1:1221231/38‑1 (MQ=255)
                                                         aCAAAACCGGACGTGAGGCGGTGATGTTCAGCTCtttt                                            <  1:1438642/38‑1 (MQ=255)
                                                         aCAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCa                          >  1:3479667/1‑56 (MQ=255)
                                                         aCAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCa                          >  1:1879672/1‑56 (MQ=255)
                                                         aCAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCAtt           <  1:5426095/71‑1 (MQ=255)
                                                          cAAAACCGGACGTGAGGCGGTGATGTTCAGCTCttttt                                           <  1:4366530/38‑1 (MQ=255)
                                                          cAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCAc                         >  1:2882481/1‑56 (MQ=255)
                                                           aaaaCCGGACGTGAGGCGGTGATGTTCAGCTCtttttt                                          >  1:3911806/1‑38 (MQ=255)
                                                           aaaaCCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATg                  <  1:4625564/62‑1 (MQ=255)
                                                           aaaaCCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTgg         >  1:160445/1‑71 (MQ=255)
                                                           aaaaCCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTgg         >  1:4104137/1‑71 (MQ=255)
                                                             aaCCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGcc       >  1:4932554/1‑71 (MQ=255)
                                                              aCCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTAcg                                       >  1:5235159/1‑38 (MQ=255)
                                                              aCCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTAcg                                       >  1:1032836/1‑38 (MQ=255)
                                                                cGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCAt            >  1:2878861/1‑63 (MQ=255)
                                                                  gACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTc              <  1:1508296/59‑1 (MQ=255)
                                                                  gACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCg  <  1:3737503/71‑1 (MQ=255)
                                                                   |                                                                     
GCAGCTTTGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCG  >  Exported/1395641‑1395777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: