Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,395,715 G→A 14.9% A141T (GCG→ACG)  setB → lactose/glucose efflux system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,395,7150GA14.9% 260.3 / 29.0 107A141T (GCG→ACG) setBlactose/glucose efflux system
Reads supporting (aligned to +/- strand):  ref base G (38/53);  new base A (9/7);  total (47/60)
Fisher's exact test for biased strand distribution p-value = 4.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 107 total aligned reads displayed.

TGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCGCTGGCT  >  Exported/1395648‑1395783
                                                                   |                                                                    
tGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGc                                                                     <  1:2859038/69‑1 (MQ=255)
tGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGc                                                                     <  1:4069707/69‑1 (MQ=255)
 ggCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCgg                                                                   <  1:2464191/70‑1 (MQ=255)
 ggCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCgg                                                                   <  1:3938887/70‑1 (MQ=255)
 ggCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCgg                                                                   <  1:655697/70‑1 (MQ=255)
  gCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTg                                                                 <  1:2601181/71‑1 (MQ=255)
    tCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGAt                                                               >  1:4926446/1‑71 (MQ=255)
    tCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGAt                                                               >  1:1455267/1‑71 (MQ=255)
     cGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATg                                                              >  1:3794624/1‑71 (MQ=255)
       aCCGCAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGtt                                                            <  1:1246216/71‑1 (MQ=255)
       aCCGCAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGtt                                                            <  1:5499068/71‑1 (MQ=255)
       aCCGCAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGtt                                                            <  1:1748280/71‑1 (MQ=255)
       aCCGCAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGtt                                                            <  1:1754783/71‑1 (MQ=255)
         cGCAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGtt                                                            >  1:4275324/1‑69 (MQ=255)
         cGCAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGtt                                                            >  1:5679529/1‑69 (MQ=255)
          gCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAg                                                         >  1:2190858/1‑71 (MQ=255)
           cTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGc                                                        >  1:3744981/1‑71 (MQ=255)
           cTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGc                                                        <  1:1596494/71‑1 (MQ=255)
           cTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGc                                                        >  1:449811/1‑71 (MQ=255)
             aaCCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGctc                                                      >  1:4711307/1‑71 (MQ=255)
              aCCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGctct                                                     >  1:2786936/1‑71 (MQ=255)
              aCCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACg                                                                    >  1:3234405/1‑56 (MQ=255)
              aCCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAgt                                                        <  1:3406017/68‑2 (MQ=255)
                ccGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCttt                                                   <  1:1777486/71‑1 (MQ=255)
                ccGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCttt                                                   <  1:4381194/71‑1 (MQ=255)
                 cGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCtttt                                                  <  1:5526964/71‑1 (MQ=255)
                 cGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCttt                                                   <  1:4016472/70‑1 (MQ=255)
                  gCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTg                                                                 <  1:4100766/55‑1 (MQ=255)
                  gCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGa                                                                >  1:2115148/1‑56 (MQ=255)
                  gCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCttttt                                                 >  1:4897041/1‑71 (MQ=255)
                  gCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCttttt                                                 <  1:2502730/71‑1 (MQ=255)
                  gCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCtt                                                    <  1:1207736/68‑1 (MQ=255)
                  gCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCAt                                                               >  1:1305554/1‑57 (MQ=255)
                   cAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCtttttt                                                <  1:3541979/71‑1 (MQ=255)
                    aaaTGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACattttt                                                >  1:2876511/1‑70 (MQ=255)
                    aaaTGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTa                                               >  1:2025824/1‑71 (MQ=255)
                        gTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTAcgcg                                           >  1:4945487/1‑71 (MQ=255)
                        gTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTAcgcg                                           >  1:3163144/1‑71 (MQ=255)
                        gTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTAcg                                             >  1:4327109/1‑69 (MQ=255)
                         tttGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCttttt                                                 <  1:4347731/64‑1 (MQ=255)
                         tttGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTa                                               <  1:661291/66‑1 (MQ=255)
                          ttgcccttgcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCt                                         >  1:576392/1‑71 (MQ=255)
                           tgcccttgcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCttttt                                                 >  1:686189/1‑62 (MQ=255)
                           tgcccttgcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCtt                                                    <  1:2221757/59‑1 (MQ=255)
                            gcccttgcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTc                                        <  1:3412457/70‑1 (MQ=255)
                            gcccttgcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTc                                        <  1:5692343/70‑1 (MQ=255)
                             cccttgcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCa                                       <  1:1567975/70‑1 (MQ=255)
                               cttgcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGt                                    >  1:3063026/1‑71 (MQ=255)
                                ttgcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGt                                                                  <  1:3018376/40‑1 (MQ=255)
                                ttgcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGt                                                                  <  1:4801565/40‑1 (MQ=255)
                                ttgcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCttttt                                                 <  1:2090912/57‑1 (MQ=255)
                                ttgcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAg                                      <  1:1125024/68‑1 (MQ=255)
                                tcgcccGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACattttt                                                <  1:4686250/56‑1 (MQ=255)
                                tcgcccGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTa                                               >  1:839972/3‑59 (MQ=255)
                                tcgcccGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTa                                               >  1:3694005/3‑59 (MQ=255)
                                 cgcccGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTAc                                              >  1:3942509/2‑59 (MQ=255)
                                  gcccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGttt                                  <  1:800569/70‑1 (MQ=255)
                                    ccGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCa                                <  1:3915962/70‑1 (MQ=255)
                                    ccGAGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGttt                                  <  1:2147932/68‑1 (MQ=255)
                                     cGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGttt                                  <  1:2228594/67‑1 (MQ=255)
                                      gTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCttttt                                                 <  1:3531149/51‑1 (MQ=255)
                                      gTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCa                                >  1:1669390/1‑68 (MQ=255)
                                      gTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTg                             <  1:5150326/71‑1 (MQ=255)
                                      gTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTg                             >  1:1913207/1‑71 (MQ=255)
                                        gAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCttt                                                   <  1:2201812/47‑1 (MQ=255)
                                         aaCATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCAc                               >  1:755256/1‑66 (MQ=255)
                                         aaCATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCAc                               >  1:4962930/1‑66 (MQ=255)
                                         aaCATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCa                          >  1:413784/1‑71 (MQ=255)
                                         aaCATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCa                          <  1:3217458/71‑1 (MQ=255)
                                         aaCATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCa                          >  1:1269982/1‑71 (MQ=255)
                                             tGCCGACAAAACCGGACGTGAGGAGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATggg                      >  1:4571971/1‑71 (MQ=255)
                                              gCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATggg                      >  1:4085080/1‑70 (MQ=255)
                                               ccGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGttt                                  <  1:4090419/57‑1 (MQ=255)
                                               ccGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGttt                                  <  1:4097122/57‑1 (MQ=255)
                                               ccGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGt                     <  1:3891430/70‑1 (MQ=255)
                                               ccGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTc                    <  1:2122605/71‑1 (MQ=255)
                                                cGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCt                                         <  1:408682/49‑1 (MQ=255)
                                                 gACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCAt                  <  1:3971058/71‑1 (MQ=255)
                                                  aCAAAACTGGACGTGAGGCGGTGATGTTCAGCTCtttt                                                  <  1:1221231/38‑1 (MQ=255)
                                                  aCAAAACCGGACGTGAGGCGGTGATGTTCAGCTCtttt                                                  <  1:1438642/38‑1 (MQ=255)
                                                  aCAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCa                                >  1:3479667/1‑56 (MQ=255)
                                                  aCAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCa                                >  1:1879672/1‑56 (MQ=255)
                                                  aCAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCAtt                 <  1:5426095/71‑1 (MQ=255)
                                                   cAAAACCGGACGTGAGGCGGTGATGTTCAGCTCttttt                                                 <  1:4366530/38‑1 (MQ=255)
                                                   cAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCAc                               >  1:2882481/1‑56 (MQ=255)
                                                    aaaaCCGGACGTGAGGCGGTGATGTTCAGCTCtttttt                                                >  1:3911806/1‑38 (MQ=255)
                                                    aaaaCCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATg                        <  1:4625564/62‑1 (MQ=255)
                                                    aaaaCCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTgg               >  1:4104137/1‑71 (MQ=255)
                                                    aaaaCCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTgg               >  1:160445/1‑71 (MQ=255)
                                                      aaCCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGcc             >  1:4932554/1‑71 (MQ=255)
                                                       aCCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTAcg                                             >  1:5235159/1‑38 (MQ=255)
                                                       aCCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTAcg                                             >  1:1032836/1‑38 (MQ=255)
                                                         cGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCAt                  >  1:2878861/1‑63 (MQ=255)
                                                           gACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTc                    <  1:1508296/59‑1 (MQ=255)
                                                           gACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCg        <  1:3737503/71‑1 (MQ=255)
                                                              gTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCGCTg     <  1:157840/71‑1 (MQ=255)
                                                               tGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCGCTgg    <  1:5035908/71‑1 (MQ=255)
                                                                 aGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCGCTGGc   <  1:4961931/70‑1 (MQ=255)
                                                                 aGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCGCTGGCt  <  1:3353607/71‑1 (MQ=255)
                                                                 aGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCGCTGGCt  <  1:4653523/71‑1 (MQ=255)
                                                                   |                                                                    
TGGCTCGACCGCTAACCCGCAAATGTTTGCCCTTGCCCGTGAACATGCCGACAAAACCGGACGTGAGGCGGTGATGTTCAGCTCTTTTTTACGCGCTCAGGTTTCACTGGCATGGGTCATTGGCCCACCGCTGGCT  >  Exported/1395648‑1395783

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: