| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,398,448 | A→C | 6.4% | I258L (ATC→CTC) ‡ | yeiQ → | predicted dehydrogenase, NAD‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,398,448 | 0 | A | C | 6.4% | 250.0 / 9.2 | 94 | I258L (ATC→CTC) ‡ | yeiQ | predicted dehydrogenase, NAD‑dependent |
| Reads supporting (aligned to +/- strand): ref base A (53/35); new base C (4/2); total (57/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
CTGGTGGAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGG > Exported/1398385‑1398517 | cttgtggAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAgtg < 1:1109242/68‑1 (MQ=255)cTGGTGGAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAgtg < 1:2667568/71‑1 (MQ=255)cTGGTGGAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAgtg < 1:3973047/71‑1 (MQ=255) ggtggAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAgtggg > 1:1165565/1‑71 (MQ=255) gtggAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAgtgggt > 1:3105828/1‑71 (MQ=255) ggAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAgtgggtgg > 1:4219094/1‑71 (MQ=255) aaaTCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGgtggtg > 1:2352294/1‑71 (MQ=255) aaTCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGtggtgg < 1:4597103/71‑1 (MQ=255) aTCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAgtgggtgg < 1:3399519/67‑1 (MQ=255) agccagcATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAg < 1:3657530/70‑1 (MQ=255) agccagcATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAg < 1:4715092/70‑1 (MQ=255) agcATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAAc > 1:781736/1‑71 (MQ=255) aTCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTtc > 1:4034143/1‑71 (MQ=255) aTCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTtc > 1:4281641/1‑71 (MQ=255) tGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAgtgggtg < 1:680593/53‑1 (MQ=255) tGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTtcgtc > 1:3344106/1‑71 (MQ=255) tGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTtcgtc > 1:3049965/1‑71 (MQ=255) gggggTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTtcgtcg > 1:5083381/1‑71 (MQ=255) gggggTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTtcgtc > 1:784404/1‑70 (MQ=255) gggggTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTtcg < 1:2204438/68‑1 (MQ=255) ggggTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTtcg < 1:4510589/67‑1 (MQ=255) ggggTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGc < 1:5491649/71‑1 (MQ=255) gTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATc < 1:210080/39‑1 (MQ=255) taaTGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGaa < 1:2570899/51‑1 (MQ=255) gAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGc > 1:5566887/1‑71 (MQ=255) aaTGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCa > 1:4194354/1‑38 (MQ=255) aaTGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAg > 1:3392752/1‑39 (MQ=255) aaTGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAg > 1:1825352/1‑39 (MQ=255) aaTGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAg > 1:1483074/1‑39 (MQ=255) aaTGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAg > 1:4272767/1‑39 (MQ=255) aaTGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCg > 1:4810909/1‑71 (MQ=255) aaTGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCg < 1:3486138/71‑1 (MQ=255) atgatgCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGt > 1:4653900/1‑71 (MQ=255) aTGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCg > 1:4601743/1‑70 (MQ=255) aTGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGt > 1:2011444/1‑71 (MQ=255) tGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCg < 1:5243313/69‑1 (MQ=255) gATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTcc > 1:4286540/1‑71 (MQ=255) aTCCCTGCGCGATTAGCTGCGAACCGTTTATCCAgtggg > 1:4298412/1‑39 (MQ=255) aTCCCTGCGCGATTAGCTGCGAACCGTTTATCCAgtggg > 1:561118/1‑39 (MQ=255) aTCCCTGCGCGATTAGCTGCGAACCGTTTATCCAgtggg > 1:964430/1‑39 (MQ=255) aTCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGa < 1:982987/46‑1 (MQ=255) aTCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGa < 1:936633/46‑1 (MQ=255) aTCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGa < 1:2904715/46‑1 (MQ=255) aTCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGa < 1:1018479/46‑1 (MQ=255) ccTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTcctgc > 1:5555090/1‑70 (MQ=255) ccTGCCCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTcctgcc > 1:1063317/1‑71 (MQ=255) cTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGaa < 1:4585255/43‑1 (MQ=255) tGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTcctgcctg > 1:4908960/1‑71 (MQ=255) tGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTcctgcctg > 1:5059235/1‑71 (MQ=255) tGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTcctgcctg > 1:3973929/1‑71 (MQ=255) tGCGCCATTGCCTGCGAACCGTTCCGTCAGTGGGTGATTGAAGAt > 1:2744881/1‑45 (MQ=255) tGCGCCATTGCCTGCGAACCGTTCCGTCAGTGGGTGATTGAAGAt > 1:178234/1‑45 (MQ=255) tGCGCCATTGCCTGCGAACCGTTCCGTCAGTGGGTGATTGAAGAt > 1:2664134/1‑45 (MQ=255) tGCGCCATTGCCTGCGAACCGTTCCGTCAGTGGGTGATTGAAGAt > 1:264899/1‑45 (MQ=255) cgcgATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGa > 1:2329784/1‑42 (MQ=255) cgcgATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTggg < 1:3198279/71‑1 (MQ=255) cgcgATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTgg < 1:1616006/70‑1 (MQ=255) cgATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGa < 1:872647/70‑1 (MQ=255) gATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAg < 1:5332707/71‑1 (MQ=255) aTTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATa > 1:902736/1‑40 (MQ=255) aTTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATa > 1:4971611/1‑40 (MQ=255) aTTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATa > 1:3543998/1‑40 (MQ=255) aTTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTgg < 1:4211032/53‑1 (MQ=255) aTTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGt < 1:2727726/57‑1 (MQ=255) tAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCg > 1:3704498/1‑71 (MQ=255) tAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCg > 1:3223872/1‑71 (MQ=255) tAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCg > 1:4926710/1‑71 (MQ=255) aGCTTCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGc > 1:1006804/1‑47 (MQ=255) gCTGCGAACCGTTTATCCAGTGTGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGc < 1:5582312/70‑1 (MQ=255) gCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGc < 1:2425866/70‑1 (MQ=255) cTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTtcgtc < 1:2067472/43‑1 (MQ=255) tGCGAACCGTTCCGTCAGTGGGTGATTGAAGATAACTtcgtt < 1:1344821/42‑2 (MQ=16) gCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTtcgt > 1:4686374/1‑40 (MQ=255) gCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCa < 1:1394008/68‑1 (MQ=255) gCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGgt > 1:2661194/1‑71 (MQ=255) gCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGgt > 1:2264952/1‑71 (MQ=255) cGAGCCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCa > 1:2516092/1‑67 (MQ=255) cGAACCGTTCCGTCAGTGGGTGATTGAAGATAACTtcgtt < 1:2285620/40‑2 (MQ=12) gAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTcctg > 1:1334294/1‑52 (MQ=255) gAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGgtgt < 1:4470716/71‑1 (MQ=255) aaCCGTTTATCCAGTGGGTGGTGGAAGATAACTtcgtc < 1:1095967/38‑1 (MQ=255) aaCCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCg < 1:1670057/63‑1 (MQ=255) aaCCGTTTATCCAGTCGGTGGTGGAAGATAACTtcgtc < 1:2734799/38‑1 (MQ=255) gTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACaaa > 1:4171660/1‑71 (MQ=255) gTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACaaa > 1:5299156/1‑71 (MQ=255) gTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACaaa > 1:5483649/1‑71 (MQ=255) gTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACaaa > 1:2523215/1‑71 (MQ=255) tttATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCg < 1:4890295/41‑1 (MQ=255) tttATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTCGGCGt > 1:253146/1‑42 (MQ=255) tATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTg > 1:2253363/1‑35 (MQ=255) tATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTg > 1:4171726/1‑35 (MQ=255) tATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTg > 1:2199018/1‑35 (MQ=255) tATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTc > 1:4482699/1‑41 (MQ=255) tATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATgg > 1:3425515/1‑71 (MQ=255) | CTGGTGGAAATCAGCCAGCATCTGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTGGGAAGTCGCAGGTGTACAAATGG > Exported/1398385‑1398517 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |