| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,401,970 | G→A | 7.3% | R187H (CGC→CAC) | spr → | predicted peptidase, outer membrane lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,401,970 | 0 | G | A | 7.3% | 276.4 / 8.2 | 96 | R187H (CGC→CAC) | spr | predicted peptidase, outer membrane lipoprotein |
| Reads supporting (aligned to +/- strand): ref base G (45/43); new base A (4/3); total (49/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.21e-01 | |||||||||||
TTATTATTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCT > Exported/1401901‑1402034 | ttattaTTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCg > 1:5171596/1‑70 (MQ=255) attattTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCg < 1:400803/68‑1 (MQ=255) attattTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCg < 1:5067055/68‑1 (MQ=255) attattTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCa < 1:5522145/70‑1 (MQ=255) ttattTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCg < 1:2026893/67‑1 (MQ=255) tattTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGc > 1:5627303/1‑70 (MQ=255) attTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCt > 1:4052654/1‑70 (MQ=255) tttCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGc > 1:2278391/1‑68 (MQ=255) ccAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCtaata < 1:4694354/70‑1 (MQ=255) ccAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCtaat < 1:4057848/69‑1 (MQ=255) atgaatgaACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGc < 1:4080478/57‑1 (MQ=255) atgaatgaACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGt > 1:370094/1‑71 (MQ=255) atgaatgaACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGt < 1:4046752/71‑1 (MQ=255) atgaatgaACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGt < 1:1971114/71‑1 (MQ=255) aatgaaCCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGc > 1:3517906/1‑54 (MQ=255) aatgaaCCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTg > 1:1020844/1‑71 (MQ=255) aatgaaCCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTg > 1:4318380/1‑71 (MQ=255) aatgaaCCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTg < 1:1904570/71‑1 (MQ=255) atgaaCCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTgg < 1:1897319/71‑1 (MQ=255) atgaaCCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTg > 1:2653920/1‑70 (MQ=255) atgaaCCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTg < 1:3764505/70‑1 (MQ=255) gaaCCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCtaat > 1:3868052/1‑58 (MQ=255) gaaCCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGAt > 1:5552251/1‑71 (MQ=255) aaCCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAAc > 1:1946384/1‑61 (MQ=255) aaCCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGAt > 1:5403289/1‑70 (MQ=255) aCCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATg < 1:1508703/70‑1 (MQ=255) cGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGc < 1:2619807/69‑1 (MQ=255) tACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCTCAGCt < 1:4352983/49‑1 (MQ=255) aCTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATc < 1:5614551/70‑1 (MQ=255) aCTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATc < 1:4622786/70‑1 (MQ=255) cTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATcc > 1:2727684/1‑70 (MQ=255) ggAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCtt > 1:3644943/1‑71 (MQ=255) gaagaaGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGa > 1:3101296/1‑59 (MQ=255) gaagaaGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCtt < 1:893403/70‑1 (MQ=255) gaagaaGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTg < 1:3425478/71‑1 (MQ=255) gaagaaGCGTTACAACGAAGCACGCCGGGTTCTAAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTg > 1:4545274/1‑71 (MQ=255) aagaagCGTTACACCGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCtt < 1:3739176/69‑1 (MQ=255) aagaagCGTTACAACGAAGCACGCCGGGTTCTCAGCCACAGCTAATAAACCGTTTGGa < 1:1021169/58‑1 (MQ=255) aagaagCGTTACAACGAAGCACGCCGGGTTCTCAGCCACAGCTAATAAACCGTTTGGa < 1:2463362/58‑1 (MQ=255) agaagCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCaa > 1:4458021/1‑62 (MQ=255) agaagCGTTACAACGAAGCACGCCGGGTTCTCAGCCACAGCTAATAAACCGTTTGGa < 1:4983359/57‑1 (MQ=255) agaagCGTTACAACGAAGCACGCCGGCTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGc > 1:4372010/1‑71 (MQ=255) gaagCGTTACAACGAAGCACGCCGGGTTCTCAGCCACAGCTAATAAACCGTTTGGATg > 1:3640984/1‑58 (MQ=255) gaagCGTTACAACGAAGCACGCCGGGTTCTCAGCCACAGCTAATAAACCGTTTGGATg > 1:1802759/1‑58 (MQ=255) gaagCGTTACAACGAAGCACGCCGGGTTCTCAGCCACAGCTAATAAACCGTTTGGATg > 1:2294106/1‑58 (MQ=255) gaagCGTTACAACGAAGCACGCCGGGTTCTCAGCCACAGCTAATAAACCGTTTGGATg > 1:5461549/1‑58 (MQ=255) aagCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTgg < 1:3282401/68‑1 (MQ=255) aagCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTa < 1:3629167/71‑1 (MQ=255) aagCGTTACAACGAAGCACGCCGGGATCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGc < 1:498355/69‑1 (MQ=255) agCGTTACAACGAAGCATGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTAt > 1:295262/1‑71 (MQ=255) cGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATcc > 1:110898/1‑71 (MQ=255) gTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTa < 1:5545843/67‑1 (MQ=255) gTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTa < 1:5524170/67‑1 (MQ=255) ttACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATc > 1:2372435/1‑68 (MQ=255) ttACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCt > 1:4384731/1‑70 (MQ=255) ttACAACGAAGCACGACGGGTTCTCAGCCGCAGCtaa < 1:3004345/37‑1 (MQ=255) tACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATcc < 1:1125459/68‑1 (MQ=255) tACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATcc < 1:1736567/68‑1 (MQ=255) aCAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAAc > 1:1385809/1‑40 (MQ=255) aCAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAAc > 1:1667769/1‑40 (MQ=255) aCAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGa > 1:1537548/1‑48 (MQ=255) aCAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGAc > 1:3015546/1‑71 (MQ=255) cAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGAc < 1:4441657/70‑1 (MQ=255) cGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCtt < 1:2889378/55‑1 (MQ=255) cGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCt > 1:4918430/1‑64 (MQ=255) cGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGt > 1:933427/1‑71 (MQ=255) cGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGt > 1:4370170/1‑71 (MQ=255) cGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGt > 1:2751229/1‑71 (MQ=255) gAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGtt < 1:3744221/71‑1 (MQ=255) aaGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTa < 1:371621/71‑1 (MQ=255) gCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTg < 1:2518243/38‑1 (MQ=255) cACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACg < 1:2576564/63‑1 (MQ=255) cACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGtt < 1:2588796/67‑1 (MQ=255) gCCGGGTTCTCAGCCGCATCTAATAAACCGTTTGGATGCaa > 1:4709240/1‑41 (MQ=255) gCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCaa > 1:3781743/1‑41 (MQ=255) gCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATcc > 1:1952772/1‑55 (MQ=255) cGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAg > 1:2190010/1‑71 (MQ=255) cGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAg < 1:4386232/71‑1 (MQ=255) gggTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCt < 1:1352235/43‑1 (MQ=255) gggTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATc < 1:1873743/51‑1 (MQ=255) gggTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATc < 1:2792390/51‑1 (MQ=255) gggTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATc < 1:512523/51‑1 (MQ=255) gggTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAg > 1:4233986/1‑70 (MQ=255) ttCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACt < 1:108118/62‑1 (MQ=255) gatcAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTg < 1:3763804/69‑1 (MQ=255) gatcAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTg < 1:11479/69‑1 (MQ=255) ctcAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGc > 1:2702969/1‑71 (MQ=255) ctcAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGc > 1:68852/1‑71 (MQ=255) tcAGCCGCAGCTAATAAACCGTTTGGATGCAATccc > 1:1589579/1‑36 (MQ=255) tcAGCCGCAGCTAATAAACCGTTTGGATGCAATccc > 1:3535552/1‑36 (MQ=255) cAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGa > 1:897816/1‑48 (MQ=255) cAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGa > 1:3957422/1‑48 (MQ=255) aGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGa > 1:2813579/1‑59 (MQ=255) aGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCt < 1:4916333/69‑1 (MQ=255) aGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCt < 1:817081/69‑1 (MQ=255) aGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCt < 1:3431154/69‑1 (MQ=255) aGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCt < 1:3895043/69‑1 (MQ=255) gCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAAc > 1:3516209/1‑55 (MQ=255) gCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAAc > 1:1923293/1‑55 (MQ=255) gCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAAc > 1:4484611/1‑55 (MQ=255) | TTATTATTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCT > Exported/1401901‑1402034 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |