Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,439,300 G→A 16.6% Y334Y (TAC→TAT ompC ← outer membrane porin protein C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,439,3000GA16.6% 561.3 / 83.9 234Y334Y (TAC→TATompCouter membrane porin protein C
Reads supporting (aligned to +/- strand):  ref base G (104/91);  new base A (21/18);  total (125/109)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 242 total aligned reads displayed.

TTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGT  >  Exported/1439232‑1439370
                                                                    |                                                                      
ttATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACg                                                                        >  1:3940912/1‑69 (MQ=255)
ttATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACg                                                                        >  1:771291/1‑69 (MQ=255)
ttATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTa                                                                      <  1:4031249/71‑1 (MQ=255)
   tCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAgg                                                                    >  1:38354/1‑70 (MQ=255)
       tgtTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAgg                                                                    <  1:1046173/66‑1 (MQ=255)
       tgtTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAgg                                                                    <  1:5355698/66‑1 (MQ=255)
         ttGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACAt                                                             >  1:5274048/1‑71 (MQ=255)
           gATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGt                                                           >  1:271094/1‑71 (MQ=255)
           gATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGt                                                           >  1:1085518/1‑71 (MQ=255)
           gATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGATTGATTTTGTAGTCAACGTAGGTGGACATGt                                                           >  1:824490/1‑71 (MQ=255)
            aTGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGtt                                                          <  1:2947650/71‑1 (MQ=255)
            aTGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGt                                                           <  1:4147216/70‑1 (MQ=255)
             tGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGttt                                                         >  1:3884185/1‑71 (MQ=255)
              gCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGtttt                                                        >  1:1636361/1‑71 (MQ=255)
               ccAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGtttt                                                        <  1:5478143/70‑1 (MQ=255)
                cAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttg                                                      <  1:2314737/71‑1 (MQ=255)
                cAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttg                                                      >  1:3092386/1‑71 (MQ=255)
                 aGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGtttt                                                        <  1:3381229/68‑1 (MQ=255)
                   cGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttgttg                                                   <  1:2661346/71‑1 (MQ=255)
                   cGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttgtt                                                    >  1:2061745/1‑70 (MQ=255)
                   cGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGCACAt                                                             >  1:2442057/1‑61 (MQ=255)
                    gTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGa                                                  <  1:4625872/71‑1 (MQ=255)
                       aCGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAag                                                >  1:4193704/1‑70 (MQ=255)
                         gAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAagtag                                             >  1:503619/1‑71 (MQ=255)
                           gTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGt                                                                       <  1:407646/43‑1 (MQ=255)
                           gTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGt                                                                       <  1:2667403/43‑1 (MQ=255)
                           gTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTa                                                                      <  1:2777514/44‑1 (MQ=255)
                           gTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAgtagta                                           <  1:3003898/71‑1 (MQ=255)
                              aaCTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAg                                                                     >  1:1019975/1‑42 (MQ=255)
                              aaCTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtaggt                                        <  1:3655631/71‑1 (MQ=255)
                              aaCTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtaggt                                        <  1:3413120/71‑1 (MQ=255)
                              aaCTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtagg                                         <  1:2133898/70‑1 (MQ=255)
                              aaCTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtagg                                         >  1:2870853/1‑70 (MQ=255)
                               aCTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtagg                                         >  1:2175317/1‑69 (MQ=255)
                                cTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACAt                                                             >  1:48516/1‑48 (MQ=255)
                                cTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACAt                                                             >  1:4341750/1‑48 (MQ=255)
                                cTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACAt                                                             >  1:4003433/1‑48 (MQ=255)
                                cTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtaggtag                                      <  1:4665887/71‑1 (MQ=255)
                                  ggTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCa                                    >  1:5450907/1‑71 (MQ=255)
                                   gTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAagtagt                                            >  1:3393273/1‑62 (MQ=255)
                                      gttgtcCAGCAGGTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACcaa                                <  1:3520931/68‑1 (MQ=18)
                                      gtcgtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaa                                >  1:2572929/1‑71 (MQ=255)
                                       tcatcCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCcaa                                >  1:2288634/4‑70 (MQ=17)
                                        catcCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAaata                                              >  1:1942366/3‑55 (MQ=18)
                                         gtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttgt                                                     >  1:1502786/1‑47 (MQ=255)
                                         gtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacat                             >  1:4390068/1‑71 (MQ=255)
                                         gtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacat                             >  1:2913728/1‑71 (MQ=255)
                                         gtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacat                             >  1:5407005/1‑71 (MQ=255)
                                         gtcCAGCAGGTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCa                                    >  1:3334123/1‑64 (MQ=17)
                                         atcCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGtttt                                                        <  1:1146107/43‑1 (MQ=21)
                                         atcCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCcaata                              <  1:335678/69‑3 (MQ=17)
                                         atcCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCcaata                              <  1:2830113/69‑3 (MQ=17)
                                          tcCAGCAGGTTGATTCTGTAGTCAACGTAGGTGGACATGttttt                                                       <  1:2523792/44‑1 (MQ=255)
                                          tcCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGtttttat                                                     >  1:1058379/1‑44 (MQ=21)
                                            cagcagGTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACcaatatcaa                          >  1:1837273/1‑71 (MQ=18)
                                            cagcagGTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACcaatatca                           <  1:4983761/70‑1 (MQ=17)
                                            caacagGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGcc                                  <  1:5209956/60‑1 (MQ=14)
                                             agcagGTTGATTTTGTAATCAACATAGGTGGACATGTTTttgttg                                                   <  1:5516660/45‑1 (MQ=17)
                                              gcagGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaaca                        >  1:1599128/1‑71 (MQ=255)
                                              gcagGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaaca                        <  1:3425900/71‑1 (MQ=255)
                                              gcagGTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACcaatatcaacg                        <  1:5693118/71‑2 (MQ=17)
                                               cagGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCcaatat                             >  1:2701219/1‑65 (MQ=17)
                                               cagGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCcaatat                             >  1:1673454/1‑65 (MQ=17)
                                                agGTTGATTTTGTAATCAACATAGGTGGACATGTTTttg                                                      >  1:3980827/1‑39 (MQ=14)
                                                agGTTGATTTTGTAATCAACATAGGTGGACATGTTTttg                                                      >  1:1902300/1‑39 (MQ=14)
                                                  gTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGa                                                  <  1:2416003/41‑1 (MQ=255)
                                                  gTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAagtagt                                            <  1:2497682/47‑1 (MQ=255)
                                                  gTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAag                                                <  1:3031492/43‑1 (MQ=255)
                                                  gTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtaggt                                        <  1:1990063/51‑1 (MQ=255)
                                                  gTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCAcc                                  <  1:4986682/57‑1 (MQ=14)
                                                   ttGATTTTGTAATCAACATAGGTGGACATGTTTttgttg                                                   <  1:4690003/39‑1 (MQ=14)
                                                   ttGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGTAttt                   <  1:4368525/71‑1 (MQ=9)
                                                    tGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaa                                >  1:1251756/1‑57 (MQ=255)
                                                    tGATTTTGTAATCAACATAGGTGGATATGTTTTTGTTGAAGTAGTAGGTCGCACcaa                                >  1:4956813/1‑57 (MQ=14)
                                                    tGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAag                                                <  1:3504904/41‑1 (MQ=14)
                                                     gATTTTGTAGTCAACGTAGGTGGACATGTTTttgtt                                                    <  1:1391926/36‑1 (MQ=255)
                                                     gATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAttt                   >  1:3949071/1‑69 (MQ=255)
                                                     gATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAttt                   >  1:5196568/1‑69 (MQ=255)
                                                     gATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAttt                   >  1:2546674/1‑69 (MQ=255)
                                                      aTTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAg                <  1:894945/71‑1 (MQ=255)
                                                      aTTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAg                <  1:4293262/71‑1 (MQ=255)
                                                      aTTTTGTAATCAACATAGGTGGACATGTTTttgttg                                                   <  1:2376032/36‑1 (MQ=11)
                                                      aTTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAgtaggt                                        >  1:316245/1‑47 (MQ=11)
                                                      aTTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAgtaggt                                        >  1:2030702/1‑47 (MQ=11)
                                                      aTTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACCAATATCAACGTAtttaac                >  1:1517902/1‑68 (MQ=17)
                                                      aTTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACCAATATCAACGTAtttaac                >  1:1787158/1‑68 (MQ=17)
                                                       ttttGTAGTCAACGTAGGTGGACATGTTTTTGTTGAag                                                >  1:2612646/1‑38 (MQ=255)
                                                        tttATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGc                                     <  1:377474/48‑1 (MQ=14)
                                                          tGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGata            >  1:4428765/1‑71 (MQ=255)
                                                           gTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACatat                     >  1:4159667/1‑61 (MQ=255)
                                                            tAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCa                 >  1:3442550/1‑64 (MQ=255)
                                                            tAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCa                 >  1:3201786/1‑64 (MQ=255)
                                                            tAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCa                 >  1:4759256/1‑64 (MQ=255)
                                                               tCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaac                         <  1:4089155/53‑1 (MQ=255)
                                                               tCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaac                         <  1:617216/53‑1 (MQ=255)
                                                                 aaCGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgt     >  1:5060699/1‑71 (MQ=255)
                                                                 aaCGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgt     >  1:4268644/1‑71 (MQ=255)
                                                                 aaCGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcg      >  1:3721277/1‑70 (MQ=255)
                                                                   cGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgtcg   >  1:701866/1‑71 (MQ=255)
                                                                    gTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgtcgt  <  1:1198628/71‑1 (MQ=255)
                                                                    |                                                                      
TTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGT  >  Exported/1439232‑1439370

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: