| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,439,318 | G→A | 10.1% | N328N (AAC→AAT) | ompC ← | outer membrane porin protein C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,439,318 | 0 | G | A | 10.1% | 517.4 / 35.6 | 197 | N328N (AAC→AAT) | ompC | outer membrane porin protein C |
| Reads supporting (aligned to +/- strand): ref base G (95/82); new base A (5/15); total (100/97) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.81e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 | |||||||||||
Only 100 of 222 total aligned reads displayed.
CAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACC > Exported/1439248‑1439383 | cAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttg < 1:2314737/71‑1 (MQ=255) cGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttgttg < 1:2661346/71‑1 (MQ=255) cGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttgtt > 1:1091368/1‑70 (MQ=255) cGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttgtt > 1:2061745/1‑70 (MQ=255) gTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGa < 1:4625872/71‑1 (MQ=255) aCGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGaa > 1:4328330/1‑69 (MQ=255) aCGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAag > 1:4193704/1‑70 (MQ=255) aCGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAag < 1:3443245/70‑1 (MQ=255) aGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAagtagt < 1:3431505/71‑1 (MQ=255) tGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGtagtag < 1:4993626/71‑1 (MQ=255) tGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGtagtag > 1:3675444/1‑71 (MQ=255) aaCTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtaggt < 1:3413120/71‑1 (MQ=255) aaCTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtagg > 1:225726/1‑70 (MQ=255) aaCTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtagg > 1:2999079/1‑70 (MQ=255) aCTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtagg > 1:2175317/1‑69 (MQ=255) cTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGa < 1:2591226/59‑1 (MQ=255) cTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGa < 1:5452275/59‑1 (MQ=255) cTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtaggtag < 1:4665887/71‑1 (MQ=255) ggTTGTCGTCCAGCAGTTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttgttg > 1:1873684/1‑56 (MQ=255) ggTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCa > 1:3062769/1‑71 (MQ=255) ggTTGTCGTCCAGCAGGTTGATTTTGTAGCCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCa > 1:1678382/1‑71 (MQ=255) gTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAagtagt > 1:3393273/1‑62 (MQ=255) gTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAagtagt > 1:3374146/1‑62 (MQ=255) ttGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGa < 1:4080371/55‑1 (MQ=255) tGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCAcc > 1:1029685/1‑70 (MQ=255) gttgtcCAGCAGGTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACcaa < 1:3520931/68‑1 (MQ=18) gttgtcCAGCAGGTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACcaa > 1:2783014/4‑71 (MQ=18) tcgtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaa > 1:73164/1‑70 (MQ=255) tcgtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaa < 1:857501/70‑1 (MQ=255) tcgtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaa < 1:2556597/70‑1 (MQ=255) cgtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaaca > 1:3199365/1‑71 (MQ=255) gtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttgt > 1:1868589/1‑47 (MQ=255) gtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacat > 1:4390068/1‑71 (MQ=255) gtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacat > 1:5407005/1‑71 (MQ=255) gtcCAGCAGGTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACcaatat > 1:4026394/1‑71 (MQ=18) atcCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCcaatat < 1:5497543/70‑1 (MQ=17) atcCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCcaata < 1:4728259/69‑3 (MQ=17) tcCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCcaatatc < 1:1550976/71‑1 (MQ=17) aacagGTTGATTTTATAGTCAACGTAGGAGGACATGTTTTTATTGAAATAGTAAGTCGCGCcaat < 1:1941922/63‑2 (MQ=12) gcagGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaaca > 1:1599128/1‑71 (MQ=255) acagGTTGATTTTATAGTCAACGTAGGTGTACATGTTTTTATTGAAATAGTAAGTCGCGCcaat < 1:4093247/63‑2 (MQ=17) cagGTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACcaatatcaacgt < 1:1798618/71‑3 (MQ=17) cagGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCcaatat > 1:2701219/1‑65 (MQ=17) agGTTGATTTTGTAATCAACATAGGTGGACATGTTTttg > 1:3980827/1‑39 (MQ=14) agGTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACCAATATCAACgta < 1:4943607/71‑1 (MQ=17) ggTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACatat < 1:3344538/71‑1 (MQ=255) gTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGa < 1:2416003/41‑1 (MQ=255) gTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAagtagt < 1:2497682/47‑1 (MQ=255) gTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAagtagt < 1:1167466/47‑1 (MQ=255) gTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaacat < 1:1992361/68‑1 (MQ=255) gTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAtt > 1:1943494/1‑71 (MQ=255) tGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaa > 1:151752/1‑57 (MQ=255) tGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGaa > 1:1638300/1‑40 (MQ=14) tGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAag < 1:3504904/41‑1 (MQ=14) gATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAttt > 1:5196568/1‑69 (MQ=255) gATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACCAATATCAACGTAtttaa > 1:77945/1‑69 (MQ=17) aTTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAg < 1:4959080/71‑1 (MQ=255) aTTTTGTAATCAACATAGGTGGACATGTTTttgttg < 1:2376032/36‑1 (MQ=11) aTTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAgtaggt > 1:316245/1‑47 (MQ=11) aTTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACCAATATCAACGTAtttaac > 1:4280307/1‑68 (MQ=17) ttttATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGc < 1:4152005/49‑1 (MQ=14) tttATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGc < 1:377474/48‑1 (MQ=14) ttGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCAcc < 1:2877197/50‑1 (MQ=255) ttGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACCAATATCAACGTAtttaac > 1:3554382/1‑65 (MQ=17) tGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAttt < 1:2087892/64‑1 (MQ=255) tGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGata > 1:4428765/1‑71 (MQ=255) gTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACatat > 1:301854/1‑61 (MQ=255) tAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCa > 1:4759256/1‑64 (MQ=255) gTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCt < 1:3939898/70‑1 (MQ=255) tCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaac < 1:2710968/53‑1 (MQ=255) tCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACcaat < 1:1357715/47‑2 (MQ=11) aaCGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAg > 1:1406370/1‑60 (MQ=255) aaCGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgt > 1:5060699/1‑71 (MQ=255) aaCGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgt > 1:2936823/1‑71 (MQ=255) cGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgtcg > 1:3828426/1‑71 (MQ=255) cGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgtcg > 1:695171/1‑71 (MQ=255) gTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaaca < 1:468273/49‑1 (MQ=255) gTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgtcgt > 1:3308875/1‑71 (MQ=255) gTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACcaatatc < 1:5426337/41‑1 (MQ=17) tGGACATGTTTTTGTTGAAGTAGTAGGTCGCACcaatatcaacg < 1:5215765/44‑2 (MQ=17) tGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAg < 1:5533531/71‑1 (MQ=255) tGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAg < 1:5571001/71‑1 (MQ=255) tGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAg < 1:2912158/71‑1 (MQ=255) ggACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGc > 1:4878407/1‑71 (MQ=255) gACATGTTTTTGTTGAAGTAGTAGGTCGCACcaatatc < 1:4858451/38‑1 (MQ=17) gACATGTTTTTGTTGAAGTAGTAGGTCGCACcaatatc < 1:5246764/38‑1 (MQ=17) aCATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaaca < 1:4563086/41‑1 (MQ=255) aTGTTTTTGTTGAAGTAGTAGGTCGCACcaatatcaacg < 1:1078902/39‑2 (MQ=18) aTGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaaca < 1:2665496/39‑1 (MQ=255) aTGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTc < 1:2126452/45‑1 (MQ=255) aTGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCa > 1:586941/1‑69 (MQ=255) tttttGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCAc < 1:1300939/67‑1 (MQ=255) tttttGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGAcc > 1:4690014/1‑71 (MQ=255) tttGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTc < 1:67730/40‑1 (MQ=255) tttGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgtc > 1:1249158/1‑54 (MQ=255) tttGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgtc > 1:1063009/1‑54 (MQ=255) tttGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGAcc > 1:5624466/1‑69 (MQ=255) ttgttgAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGat > 1:2616858/1‑44 (MQ=255) ttgttgAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgt > 1:4215523/1‑52 (MQ=255) ttgttgAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgt > 1:3744766/1‑52 (MQ=255) | CAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACC > Exported/1439248‑1439383 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |