Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,439,330 G→A 11.0% T324T (ACC→ACT ompC ← outer membrane porin protein C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,439,3300GA11.0% 400.1 / 29.6 154T324T (ACC→ACTompCouter membrane porin protein C
Reads supporting (aligned to +/- strand):  ref base G (77/60);  new base A (3/14);  total (80/74)
Fisher's exact test for biased strand distribution p-value = 3.68e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 193 total aligned reads displayed.

TGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTA  >  Exported/1439260‑1439399
                                                                      |                                                                     
tGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGtagtag                                                                       <  1:4993626/71‑1 (MQ=255)
tGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGtagtag                                                                       >  1:3675444/1‑71 (MQ=255)
  aaCTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtagg                                                                      >  1:2870853/1‑70 (MQ=255)
  aaCTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtagg                                                                      >  1:3422331/1‑70 (MQ=255)
  aaCTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtagg                                                                      >  1:3529130/1‑70 (MQ=255)
    cTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtaggtag                                                                   <  1:4665887/71‑1 (MQ=255)
      ggTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCa                                                                 >  1:5450907/1‑71 (MQ=255)
      ggTTGTCGTCCAGCAGGTTGATTTTGTAGCCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCa                                                                 >  1:1678382/1‑71 (MQ=255)
        ttGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCAcc                                                               <  1:2695107/71‑1 (MQ=255)
         tGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCAcc                                                               >  1:1029685/1‑70 (MQ=255)
          gttgtcCAGCAGGTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACcaa                                                             >  1:2783014/4‑71 (MQ=18)
          gtcgtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaa                                                             >  1:2572929/1‑71 (MQ=255)
          gtcgtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaa                                                             >  1:5410912/1‑71 (MQ=255)
          gtcgtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACca                                                              <  1:266456/70‑1 (MQ=255)
           tcgtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaa                                                             <  1:857501/70‑1 (MQ=255)
           tcgtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaa                                                             >  1:73164/1‑70 (MQ=255)
             gtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacat                                                          >  1:2913728/1‑71 (MQ=255)
             atcCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCcaatat                                                          <  1:4384674/70‑1 (MQ=17)
             atcCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCcaata                                                           <  1:4728259/69‑3 (MQ=17)
                cagcagGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtaggtag                                                                   >  1:5512792/1‑59 (MQ=255)
                cagcagGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtaggtag                                                                   >  1:1146778/1‑59 (MQ=255)
                cagcagGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaa                                                       >  1:2143839/1‑71 (MQ=255)
                cagcagGTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACcaatatcaa                                                       >  1:1837273/1‑71 (MQ=18)
                cagcagGTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACcaatatca                                                        <  1:4983761/70‑1 (MQ=17)
                 aacagGTTGATTTTATAGTCAACGTAGGAGGACATGTTTTTATTGAAATAGTAAGTCGCGCcaat                                                            <  1:1941922/63‑2 (MQ=12)
                  gcagGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaaca                                                     >  1:3324712/1‑71 (MQ=255)
                  gcagGTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACcaatatcaacg                                                     <  1:5693118/71‑2 (MQ=17)
                  acagGTTGATTTTATAGTCAACGTAGGTGTACATGTTTTTATTGAAATAGTAAGTCGCGCcaat                                                            <  1:4093247/63‑2 (MQ=17)
                     ggTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACatat                                                  <  1:3344538/71‑1 (MQ=255)
                      gTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtaggt                                                                     <  1:4643368/51‑1 (MQ=255)
                      gTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaacat                                                    <  1:1992361/68‑1 (MQ=255)
                      gTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCAcc                                                               <  1:93082/57‑1 (MQ=14)
                        tGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaa                                                             >  1:1251756/1‑57 (MQ=255)
                        tGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaa                                                             >  1:151752/1‑57 (MQ=255)
                        tGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAttt                                                >  1:688004/1‑70 (MQ=255)
                        tGATTTTGTAATCAACATAGGTGGATATGTTTTTGTTGAAGTAGTAGGTCGCACcaa                                                             >  1:4956813/1‑57 (MQ=14)
                         gATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAttt                                                >  1:3949071/1‑69 (MQ=255)
                         gATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAttt                                                >  1:2546674/1‑69 (MQ=255)
                         gATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAttt                                                >  1:3398675/1‑69 (MQ=255)
                         gATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAttt                                                >  1:5196568/1‑69 (MQ=255)
                         gATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAtt                                                 >  1:2883354/1‑68 (MQ=255)
                         gATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACCAATATCAACGTAtttaa                                              >  1:77945/1‑69 (MQ=17)
                          aTTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAg                                             <  1:1056200/71‑1 (MQ=255)
                          aTTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAg                                             <  1:40485/71‑1 (MQ=255)
                          aTTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAg                                             <  1:4293262/71‑1 (MQ=255)
                          aTTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAg                                             <  1:894945/71‑1 (MQ=255)
                          aTTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAgtaggt                                                                     >  1:5070657/1‑47 (MQ=11)
                          aTTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAgtaggt                                                                     >  1:316245/1‑47 (MQ=11)
                          aTTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAgtaggt                                                                     >  1:2214622/1‑47 (MQ=11)
                          aTTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACCAATATCAACGTAtttaac                                             >  1:1787158/1‑68 (MQ=17)
                          aTTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACCAATATCAACGTAtttaac                                             >  1:3995406/1‑68 (MQ=17)
                           ttttATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGc                                                                  <  1:4152005/49‑1 (MQ=14)
                            tttATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGc                                                                  <  1:377474/48‑1 (MQ=14)
                              tGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAttt                                                <  1:5602575/64‑1 (MQ=255)
                              tGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAttt                                                <  1:2087892/64‑1 (MQ=255)
                              tGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGata                                         >  1:4428765/1‑71 (MQ=255)
                               gTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACatat                                                  >  1:2402464/1‑61 (MQ=255)
                                tAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCa                                              >  1:3442550/1‑64 (MQ=255)
                                tAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCa                                              >  1:3201786/1‑64 (MQ=255)
                                  gTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCt                                      <  1:3939898/70‑1 (MQ=255)
                                   tCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaac                                                      <  1:2710968/53‑1 (MQ=255)
                                   tCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaac                                                      <  1:617216/53‑1 (MQ=255)
                                   tCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACcaat                                                            <  1:1966581/47‑2 (MQ=11)
                                   tCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACcaat                                                            <  1:1357715/47‑2 (MQ=11)
                                     aaCGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgt                                  >  1:4268644/1‑71 (MQ=255)
                                       cGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgtcg                                >  1:3828426/1‑71 (MQ=255)
                                       cGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgtcg                                >  1:701866/1‑71 (MQ=255)
                                        gTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaaca                                                     <  1:468273/49‑1 (MQ=255)
                                        gTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgtcgt                               >  1:1200553/1‑71 (MQ=255)
                                        gTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgtcgt                               >  1:242220/1‑71 (MQ=255)
                                         tAGGTGGAGATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCt                                      <  1:4724332/63‑1 (MQ=255)
                                         tAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGa                                           >  1:5536702/1‑58 (MQ=255)
                                            gTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTa                           <  1:2999684/71‑1 (MQ=255)
                                             tGGACATGTTTTTGTTGAAGTAGTAGGTCGCACcaatatcaacg                                                     <  1:2629923/44‑2 (MQ=17)
                                             tGGACATGTTTTTGTTGAAGTAGTAGGTCGCACcaatatcaacg                                                     <  1:5215765/44‑2 (MQ=17)
                                              ggACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGc                         >  1:4878407/1‑71 (MQ=255)
                                               gACATGTTTTTGTTGAAGTAGTAGGTCGCACcaatatc                                                         <  1:5246764/38‑1 (MQ=17)
                                                aCATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaaca                                                     <  1:4563086/41‑1 (MQ=255)
                                                  aTGTTTTTGTTGAAGTAGTAGGTCGCACcaatatcaacg                                                     <  1:1078902/39‑2 (MQ=18)
                                                  aTGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTc                                               <  1:2126452/45‑1 (MQ=255)
                                                  aTGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCa                       >  1:1789429/1‑69 (MQ=255)
                                                  aTGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCa                       >  1:3183673/1‑69 (MQ=255)
                                                   tGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgtcgtc                              >  1:1336515/1‑61 (MQ=255)
                                                    gTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATc                                       <  1:1599615/51‑1 (MQ=255)
                                                     tttttGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCAc                      <  1:5472368/67‑1 (MQ=255)
                                                       tttGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTc                                               <  1:67730/40‑1 (MQ=255)
                                                       tttGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgtc                                 >  1:1520426/1‑54 (MQ=255)
                                                       tttGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgtc                                 >  1:4915154/1‑54 (MQ=255)
                                                       tttGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGAcc                  >  1:362538/1‑69 (MQ=255)
                                                        ttgttgAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgt                                  >  1:4812035/1‑52 (MQ=255)
                                                        ttgttgAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgt                                  >  1:3744766/1‑52 (MQ=255)
                                                              aaGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGttttt         <  1:1576570/71‑1 (MQ=255)
                                                                 tagtagGTAGCACCAACATCAACATATTTCAGGATATCTtcgtcgt                               <  1:2266937/46‑1 (MQ=255)
                                                                  agtagGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCt     >  1:4183742/1‑71 (MQ=255)
                                                                   gtaggtagCACCAACATCAACATATTTCAGGATATCTtcgtcgtcg                             >  1:3195482/1‑46 (MQ=255)
                                                                   gtaggtagCACCAACATCAACATATTTCAGGATATCTTcgtcgtcgt                            <  1:3049922/47‑1 (MQ=255)
                                                                   gtaggtagCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCtt    >  1:2565608/1‑71 (MQ=255)
                                                                   gtaggtagCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCt     <  1:2965075/70‑1 (MQ=255)
                                                                   gtaggtagCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCt     >  1:1745110/1‑70 (MQ=255)
                                                                    taggtagCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCttt   <  1:534830/71‑1 (MQ=255)
                                                                      |                                                                     
TGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTA  >  Exported/1439260‑1439399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: