Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | Exported | 1,439,333 | A→C | 29.0% | A323A (GCT→GCG) | ompC ← | outer membrane porin protein C |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | Exported | 1,439,333 | 0 | A | C | 29.0% | 248.3 / 139.7 | 162 | A323A (GCT→GCG) | ompC | outer membrane porin protein C |
Reads supporting (aligned to +/- strand): ref base A (67/48); new base C (14/33); total (81/81) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.67e-03 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 |
Only 100 of 177 total aligned reads displayed.
CTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTA > Exported/1439264‑1439399 | ggTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCa > 1:3062769/1‑71 (MQ=255) ggTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCa > 1:5450907/1‑71 (MQ=255) ggTTGTCGTCCAGCAGGTTGATTTTGTAGCCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCa > 1:1678382/1‑71 (MQ=255) tGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCAcc > 1:1029685/1‑70 (MQ=255) gttgtcCAGCAGGTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACcaa < 1:3520931/68‑1 (MQ=18) gtcgtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaa > 1:2572929/1‑71 (MQ=255) gtcgtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaa > 1:5410912/1‑71 (MQ=255) gtcgtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACca < 1:266456/70‑1 (MQ=255) tcgtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaa < 1:857501/70‑1 (MQ=255) tcgtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaa < 1:2556597/70‑1 (MQ=255) cgtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaaca > 1:3199365/1‑71 (MQ=255) catcCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCca < 1:2721209/66‑1 (MQ=17) gtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacat > 1:4390068/1‑71 (MQ=255) gtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacat > 1:5407005/1‑71 (MQ=255) atcCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCcaatat < 1:5497543/70‑1 (MQ=17) atcCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCcaata < 1:2830113/69‑3 (MQ=17) cagcagGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtaggtag > 1:1146778/1‑59 (MQ=255) cagcagGTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACcaatatcaa > 1:1837273/1‑71 (MQ=18) cagcagGTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACcaatatca < 1:4983761/70‑1 (MQ=17) caacagGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGcc < 1:5209956/60‑1 (MQ=14) gcagGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaaca > 1:3324712/1‑71 (MQ=255) gcagGTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACcaatatcaacg < 1:5693118/71‑2 (MQ=17) acagGTTGATTTTATAGTCAACGTAGGTGTACATGTTTTTATTGAAATAGTAAGTCGCGCcaat < 1:4093247/63‑2 (MQ=17) cagGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCcaatat > 1:2701219/1‑65 (MQ=17) agGTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACCAATATCAACgta < 1:5221442/71‑1 (MQ=17) ggTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACatat < 1:3344538/71‑1 (MQ=255) gTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaacat < 1:3945616/68‑1 (MQ=255) gTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaacat < 1:1992361/68‑1 (MQ=255) gTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAtt > 1:1943494/1‑71 (MQ=255) gTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCAcc < 1:93082/57‑1 (MQ=14) ttGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGTAttt < 1:4368525/71‑1 (MQ=9) tGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaa > 1:151752/1‑57 (MQ=255) tGATTTTGTAATCAACATAGGTGGATATGTTTTTGTTGAAGTAGTAGGTCGCACcaa > 1:4956813/1‑57 (MQ=14) gATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAttt > 1:2546674/1‑69 (MQ=255) gATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAttt > 1:3398675/1‑69 (MQ=255) gATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAttt > 1:5196568/1‑69 (MQ=255) gATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACCAATATCAACGTAtttaa > 1:77945/1‑69 (MQ=17) aTTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAg < 1:40485/71‑1 (MQ=255) aTTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAg < 1:894945/71‑1 (MQ=255) aTTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAg < 1:1056200/71‑1 (MQ=255) aTTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACCAATATCAACGTAtttaac > 1:4280307/1‑68 (MQ=17) aTTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACCAATATCAACGTAtttaac > 1:3995406/1‑68 (MQ=17) ttttATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGc < 1:4152005/49‑1 (MQ=14) tttATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGc < 1:377474/48‑1 (MQ=14) ttGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCAcc < 1:2877197/50‑1 (MQ=255) tGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAttt < 1:5602575/64‑1 (MQ=255) gTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACatat > 1:2402464/1‑61 (MQ=255) tAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCa > 1:4759256/1‑64 (MQ=255) tAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCa > 1:3442550/1‑64 (MQ=255) tCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaac < 1:617216/53‑1 (MQ=255) tCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaac < 1:3838737/53‑1 (MQ=255) aaCGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAg > 1:1406370/1‑60 (MQ=255) aaCGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgt > 1:5060699/1‑71 (MQ=255) aaCGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgt > 1:2936823/1‑71 (MQ=255) aaCGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtc > 1:4478032/1‑69 (MQ=255) cGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgtcg > 1:695171/1‑71 (MQ=255) cGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgtcg > 1:3828426/1‑71 (MQ=255) gTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgtcgt > 1:242220/1‑71 (MQ=255) gTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgtcgt > 1:3308875/1‑71 (MQ=255) gTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgtcgt > 1:1200553/1‑71 (MQ=255) tAGGTGGAGATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCt < 1:4724332/63‑1 (MQ=255) tAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGa > 1:5536702/1‑58 (MQ=255) gTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTa < 1:5314955/71‑1 (MQ=255) gTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTa < 1:2999684/71‑1 (MQ=255) tGGACATGTTTTTGTTGAAGTAGTAGGTCGCACcaatatcaacg < 1:2629923/44‑2 (MQ=17) tGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTa < 1:210962/70‑1 (MQ=255) tGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAg < 1:5533531/71‑1 (MQ=255) tGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAg < 1:2912158/71‑1 (MQ=255) gACATGTTTTTGTTGAAGTAGTAGGTCGCACcaatatc < 1:755996/38‑1 (MQ=17) gACATGTTTTTGTTGAAGTAGTAGGTCGCACcaatatc < 1:4858451/38‑1 (MQ=17) aCATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaaca < 1:4563086/41‑1 (MQ=255) aTGTTTTTGTTGAAGTAGTAGGTCGCACcaatatcaacg < 1:1078902/39‑2 (MQ=18) aTGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaaca < 1:2665496/39‑1 (MQ=255) aTGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTc > 1:1873379/1‑45 (MQ=255) aTGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTc < 1:2126452/45‑1 (MQ=255) aTGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCa > 1:1789429/1‑69 (MQ=255) aTGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCa > 1:3183673/1‑69 (MQ=255) gTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAg > 1:467350/1‑45 (MQ=255) gTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATc < 1:1599615/51‑1 (MQ=255) tttttGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCAc < 1:1300939/67‑1 (MQ=255) tttttGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCAc < 1:687909/67‑1 (MQ=255) tttttGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGAcc > 1:4690014/1‑71 (MQ=255) tttGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTc < 1:67730/40‑1 (MQ=255) tttGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgtc > 1:1520426/1‑54 (MQ=255) tttGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgtc > 1:1249158/1‑54 (MQ=255) tttGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgtc > 1:4651251/1‑54 (MQ=255) tttGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgtc > 1:4915154/1‑54 (MQ=255) tttGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGAcc > 1:362538/1‑69 (MQ=255) ttgttgAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgt > 1:4215523/1‑52 (MQ=255) ttgttgAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgt > 1:3744766/1‑52 (MQ=255) aaGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGttttt < 1:1576570/71‑1 (MQ=255) aaGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGtttt > 1:712379/1‑70 (MQ=255) tagtagGTAGCACCAACATCAACATATTTCAGGATATCTtcgtcgt < 1:2266937/46‑1 (MQ=255) tagtagGTAGCACCAACATCAACATATTTCAGGATATCTtcgtcgt < 1:1754303/46‑1 (MQ=255) agtagGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCt > 1:4183742/1‑71 (MQ=255) gtaggtagCACCAACATCAACATATTTCAGGATATCTtcgtcgtcg > 1:1704965/1‑46 (MQ=255) gtaggtagCACCAACATCAACATATTTCAGGATATCTTcgtcgtcgt < 1:3049922/47‑1 (MQ=255) taggtagCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCttt < 1:1338689/71‑1 (MQ=255) ggtagCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTa < 1:4879616/70‑1 (MQ=255) agCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGAccc < 1:4032382/52‑1 (MQ=255) | CTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTA > Exported/1439264‑1439399 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |