Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,439,333 A→C 29.0% A323A (GCT→GCG ompC ← outer membrane porin protein C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,439,3330AC29.0% 248.3 / 139.7 162A323A (GCT→GCGompCouter membrane porin protein C
Reads supporting (aligned to +/- strand):  ref base A (67/48);  new base C (14/33);  total (81/81)
Fisher's exact test for biased strand distribution p-value = 1.67e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 177 total aligned reads displayed.

CTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTA  >  Exported/1439264‑1439399
                                                                     |                                                                  
  ggTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCa                                                                 >  1:3062769/1‑71 (MQ=255)
  ggTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCa                                                                 >  1:5450907/1‑71 (MQ=255)
  ggTTGTCGTCCAGCAGGTTGATTTTGTAGCCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCa                                                                 >  1:1678382/1‑71 (MQ=255)
     tGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCAcc                                                               >  1:1029685/1‑70 (MQ=255)
      gttgtcCAGCAGGTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACcaa                                                             <  1:3520931/68‑1 (MQ=18)
      gtcgtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaa                                                             >  1:2572929/1‑71 (MQ=255)
      gtcgtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaa                                                             >  1:5410912/1‑71 (MQ=255)
      gtcgtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACca                                                              <  1:266456/70‑1 (MQ=255)
       tcgtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaa                                                             <  1:857501/70‑1 (MQ=255)
       tcgtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaa                                                             <  1:2556597/70‑1 (MQ=255)
        cgtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaaca                                                           >  1:3199365/1‑71 (MQ=255)
        catcCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCca                                                              <  1:2721209/66‑1 (MQ=17)
         gtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacat                                                          >  1:4390068/1‑71 (MQ=255)
         gtcCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacat                                                          >  1:5407005/1‑71 (MQ=255)
         atcCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCcaatat                                                          <  1:5497543/70‑1 (MQ=17)
         atcCAACAGGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCcaata                                                           <  1:2830113/69‑3 (MQ=17)
            cagcagGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAgtaggtag                                                                   >  1:1146778/1‑59 (MQ=255)
            cagcagGTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACcaatatcaa                                                       >  1:1837273/1‑71 (MQ=18)
            cagcagGTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACcaatatca                                                        <  1:4983761/70‑1 (MQ=17)
            caacagGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGcc                                                               <  1:5209956/60‑1 (MQ=14)
              gcagGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaaca                                                     >  1:3324712/1‑71 (MQ=255)
              gcagGTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACcaatatcaacg                                                     <  1:5693118/71‑2 (MQ=17)
              acagGTTGATTTTATAGTCAACGTAGGTGTACATGTTTTTATTGAAATAGTAAGTCGCGCcaat                                                            <  1:4093247/63‑2 (MQ=17)
               cagGTTGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCcaatat                                                          >  1:2701219/1‑65 (MQ=17)
                agGTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACCAATATCAACgta                                                   <  1:5221442/71‑1 (MQ=17)
                 ggTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACatat                                                  <  1:3344538/71‑1 (MQ=255)
                  gTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaacat                                                    <  1:3945616/68‑1 (MQ=255)
                  gTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaacat                                                    <  1:1992361/68‑1 (MQ=255)
                  gTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAtt                                                 >  1:1943494/1‑71 (MQ=255)
                  gTTGATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCAcc                                                               <  1:93082/57‑1 (MQ=14)
                   ttGATTTTATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGCGCCAATATCGGCGTAttt                                                <  1:4368525/71‑1 (MQ=9)
                    tGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaa                                                             >  1:151752/1‑57 (MQ=255)
                    tGATTTTGTAATCAACATAGGTGGATATGTTTTTGTTGAAGTAGTAGGTCGCACcaa                                                             >  1:4956813/1‑57 (MQ=14)
                     gATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAttt                                                >  1:2546674/1‑69 (MQ=255)
                     gATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAttt                                                >  1:3398675/1‑69 (MQ=255)
                     gATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAttt                                                >  1:5196568/1‑69 (MQ=255)
                     gATTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACCAATATCAACGTAtttaa                                              >  1:77945/1‑69 (MQ=17)
                      aTTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAg                                             <  1:40485/71‑1 (MQ=255)
                      aTTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAg                                             <  1:894945/71‑1 (MQ=255)
                      aTTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAg                                             <  1:1056200/71‑1 (MQ=255)
                      aTTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACCAATATCAACGTAtttaac                                             >  1:4280307/1‑68 (MQ=17)
                      aTTTTGTAATCAACATAGGTGGACATGTTTTTGTTGAAGTAGTAGGTCGCACCAATATCAACGTAtttaac                                             >  1:3995406/1‑68 (MQ=17)
                       ttttATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGc                                                                  <  1:4152005/49‑1 (MQ=14)
                        tttATAGTCAACGTAGGTGGACATGTTTTTATTGAAATAGTAAGTCGc                                                                  <  1:377474/48‑1 (MQ=14)
                         ttGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCAcc                                                               <  1:2877197/50‑1 (MQ=255)
                          tGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATAttt                                                <  1:5602575/64‑1 (MQ=255)
                           gTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACatat                                                  >  1:2402464/1‑61 (MQ=255)
                            tAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCa                                              >  1:4759256/1‑64 (MQ=255)
                            tAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCa                                              >  1:3442550/1‑64 (MQ=255)
                               tCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaac                                                      <  1:617216/53‑1 (MQ=255)
                               tCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaac                                                      <  1:3838737/53‑1 (MQ=255)
                                 aaCGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAg                                             >  1:1406370/1‑60 (MQ=255)
                                 aaCGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgt                                  >  1:5060699/1‑71 (MQ=255)
                                 aaCGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgt                                  >  1:2936823/1‑71 (MQ=255)
                                 aaCGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtc                                    >  1:4478032/1‑69 (MQ=255)
                                   cGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgtcg                                >  1:695171/1‑71 (MQ=255)
                                   cGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgtcg                                >  1:3828426/1‑71 (MQ=255)
                                    gTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgtcgt                               >  1:242220/1‑71 (MQ=255)
                                    gTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgtcgt                               >  1:3308875/1‑71 (MQ=255)
                                    gTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgtcgt                               >  1:1200553/1‑71 (MQ=255)
                                     tAGGTGGAGATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCt                                      <  1:4724332/63‑1 (MQ=255)
                                     tAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGa                                           >  1:5536702/1‑58 (MQ=255)
                                        gTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTa                           <  1:5314955/71‑1 (MQ=255)
                                        gTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTa                           <  1:2999684/71‑1 (MQ=255)
                                         tGGACATGTTTTTGTTGAAGTAGTAGGTCGCACcaatatcaacg                                                     <  1:2629923/44‑2 (MQ=17)
                                         tGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTa                           <  1:210962/70‑1 (MQ=255)
                                         tGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAg                          <  1:5533531/71‑1 (MQ=255)
                                         tGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAg                          <  1:2912158/71‑1 (MQ=255)
                                           gACATGTTTTTGTTGAAGTAGTAGGTCGCACcaatatc                                                         <  1:755996/38‑1 (MQ=17)
                                           gACATGTTTTTGTTGAAGTAGTAGGTCGCACcaatatc                                                         <  1:4858451/38‑1 (MQ=17)
                                            aCATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaaca                                                     <  1:4563086/41‑1 (MQ=255)
                                              aTGTTTTTGTTGAAGTAGTAGGTCGCACcaatatcaacg                                                     <  1:1078902/39‑2 (MQ=18)
                                              aTGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaaca                                                     <  1:2665496/39‑1 (MQ=255)
                                              aTGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTc                                               >  1:1873379/1‑45 (MQ=255)
                                              aTGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTc                                               <  1:2126452/45‑1 (MQ=255)
                                              aTGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCa                       >  1:1789429/1‑69 (MQ=255)
                                              aTGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCa                       >  1:3183673/1‑69 (MQ=255)
                                                gTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAg                                             >  1:467350/1‑45 (MQ=255)
                                                gTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATc                                       <  1:1599615/51‑1 (MQ=255)
                                                 tttttGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCAc                      <  1:1300939/67‑1 (MQ=255)
                                                 tttttGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCAc                      <  1:687909/67‑1 (MQ=255)
                                                 tttttGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGAcc                  >  1:4690014/1‑71 (MQ=255)
                                                   tttGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTc                                               <  1:67730/40‑1 (MQ=255)
                                                   tttGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgtc                                 >  1:1520426/1‑54 (MQ=255)
                                                   tttGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgtc                                 >  1:1249158/1‑54 (MQ=255)
                                                   tttGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgtc                                 >  1:4651251/1‑54 (MQ=255)
                                                   tttGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgtc                                 >  1:4915154/1‑54 (MQ=255)
                                                   tttGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGAcc                  >  1:362538/1‑69 (MQ=255)
                                                    ttgttgAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgt                                  >  1:4215523/1‑52 (MQ=255)
                                                    ttgttgAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTtcgt                                  >  1:3744766/1‑52 (MQ=255)
                                                          aaGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGttttt         <  1:1576570/71‑1 (MQ=255)
                                                          aaGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGtttt          >  1:712379/1‑70 (MQ=255)
                                                             tagtagGTAGCACCAACATCAACATATTTCAGGATATCTtcgtcgt                               <  1:2266937/46‑1 (MQ=255)
                                                             tagtagGTAGCACCAACATCAACATATTTCAGGATATCTtcgtcgt                               <  1:1754303/46‑1 (MQ=255)
                                                              agtagGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCt     >  1:4183742/1‑71 (MQ=255)
                                                               gtaggtagCACCAACATCAACATATTTCAGGATATCTtcgtcgtcg                             >  1:1704965/1‑46 (MQ=255)
                                                               gtaggtagCACCAACATCAACATATTTCAGGATATCTTcgtcgtcgt                            <  1:3049922/47‑1 (MQ=255)
                                                                taggtagCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCttt   <  1:1338689/71‑1 (MQ=255)
                                                                  ggtagCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTa  <  1:4879616/70‑1 (MQ=255)
                                                                     agCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGAccc                 <  1:4032382/52‑1 (MQ=255)
                                                                     |                                                                  
CTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTA  >  Exported/1439264‑1439399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: