| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,439,402 | C→T | 13.9% | Q300Q (CAG→CAA) | ompC ← | outer membrane porin protein C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,439,402 | 0 | C | T | 13.9% | 325.2 / 31.3 | 122 | Q300Q (CAG→CAA) | ompC | outer membrane porin protein C |
| Reads supporting (aligned to +/- strand): ref base C (47/58); new base T (9/8); total (56/66) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.05e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.50e-01 | |||||||||||
Only 100 of 123 total aligned reads displayed.
GCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTG > Exported/1439334‑1439472 | ccAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACt < 1:2473191/67‑1 (MQ=255) ccAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACt < 1:770152/67‑1 (MQ=255) aacatcaacatATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGc > 1:4761033/1‑67 (MQ=255) acatcaacatATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGt > 1:1031199/1‑70 (MQ=255) catcaacatATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACt < 1:3878535/63‑1 (MQ=255) tcaacatATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGc < 1:2605771/71‑1 (MQ=255) aacatATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGcc < 1:5253107/70‑1 (MQ=255) aacatATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGc > 1:123892/1‑69 (MQ=255) acatATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAg > 1:222512/1‑71 (MQ=255) acatATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAg > 1:2539654/1‑71 (MQ=255) atatTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAgg > 1:192306/1‑70 (MQ=255) atatTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAgg > 1:1765487/1‑70 (MQ=255) tttCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGt > 1:4671429/1‑59 (MQ=255) tttCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGggacg < 1:1095544/71‑1 (MQ=255) tttCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGggacg > 1:668731/1‑71 (MQ=255) tttCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGggacg < 1:4508043/71‑1 (MQ=255) tttCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGggacg > 1:3325632/1‑71 (MQ=255) tttCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGggac < 1:4286671/70‑1 (MQ=255) tttCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGggac < 1:4979120/70‑1 (MQ=255) ttCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGggacg > 1:3540881/1‑70 (MQ=255) tCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTg > 1:2617326/1‑52 (MQ=255) tCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAgg < 1:3578828/65‑1 (MQ=255) tCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAgg < 1:1319975/65‑1 (MQ=255) aGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGgacggacg < 1:2320555/71‑1 (MQ=255) gATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCa < 1:542678/71‑1 (MQ=255) gATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCa < 1:1458373/71‑1 (MQ=255) gATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCa < 1:135816/71‑1 (MQ=255) tatCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCa < 1:4449484/69‑1 (MQ=255) atCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGc < 1:4265907/54‑1 (MQ=255) atCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGAc > 1:4839122/1‑71 (MQ=255) tCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAg > 1:1088240/1‑47 (MQ=255) tCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAg > 1:2318881/1‑47 (MQ=255) ttCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCg > 1:2203836/1‑70 (MQ=255) cgtcgtcgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCg < 1:2602749/68‑1 (MQ=255) cgtcgtcgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCg < 1:1738365/68‑1 (MQ=255) cgtcgtcgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGa < 1:4591558/69‑1 (MQ=255) cgtcgtcgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAg > 1:1134832/1‑71 (MQ=255) gtcgtcgtAGCCACGACCCAGGTTTTTACCTTTGGACTGCAGGTAAGCCAGGGACGGACGCAGACCGaa < 1:2625254/69‑1 (MQ=255) gtcgtcgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGt > 1:3647463/1‑71 (MQ=255) tcgtcgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGcc < 1:1669508/48‑1 (MQ=255) gtcgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGa < 1:1833562/71‑1 (MQ=255) gtcgtAGCAACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAg > 1:1661947/1‑48 (MQ=255) cgtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAAc > 1:2020717/1‑71 (MQ=255) gtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAAc < 1:1064020/70‑1 (MQ=255) gtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAAc < 1:4714787/70‑1 (MQ=255) gtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACt > 1:4348782/1‑71 (MQ=255) gtAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGAAGACCGAAGTCGAAc < 1:3198147/70‑1 (MQ=255) gCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTgg < 1:5365246/70‑1 (MQ=255) gCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTgg < 1:2769725/70‑1 (MQ=255) gCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTgg < 1:4766235/70‑1 (MQ=255) gCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTgg < 1:5136905/70‑1 (MQ=255) ccACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAgg < 1:4403093/42‑1 (MQ=255) aCGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACt > 1:4915708/1‑71 (MQ=255) ccAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGggacg < 1:4215898/39‑1 (MQ=255) ccAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAg < 1:3641211/46‑1 (MQ=255) aGGTTTTTACCTTTAGACTGCAGGTAAGCCAGggacgga > 1:3386334/1‑39 (MQ=255) aGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAAc < 1:5095140/71‑1 (MQ=255) ggTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGaa > 1:3569540/1‑55 (MQ=255) ggTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACa < 1:3138662/71‑1 (MQ=255) gTTTTTACCTTTAGACTGCAGGTAAGCCAGGgacggacg < 1:915468/39‑1 (MQ=255) tttttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGaa < 1:2070705/47‑1 (MQ=255) ttttACCTTTAGACTGCAGGTAAGCCAGGgacggacg < 1:2234658/37‑1 (MQ=255) ttttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCg > 1:104976/1‑50 (MQ=255) ttttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCg > 1:152100/1‑50 (MQ=255) ttttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCg > 1:3046131/1‑50 (MQ=255) ttttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCt > 1:5083192/1‑71 (MQ=255) ttttACCTTTAGACTGCAGGTAAGCCAGCGACGGACGCAGACCGAAGTCg > 1:282336/1‑50 (MQ=255) tttACCTTTAGACTGCAGGTAAGCCAGGgacggacg < 1:957795/36‑1 (MQ=255) tttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGaa > 1:5096822/1‑51 (MQ=255) tttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGc < 1:5545305/63‑1 (MQ=255) tttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCtt > 1:3856771/1‑71 (MQ=255) tttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCtt > 1:359277/1‑71 (MQ=255) tttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCtt > 1:1710827/1‑71 (MQ=255) tttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCt < 1:1406890/70‑1 (MQ=255) tttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCt < 1:2948643/70‑1 (MQ=255) cttACCTTTAGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACt > 1:3008237/2‑53 (MQ=12) ttACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGt > 1:2741014/1‑46 (MQ=255) tACCTTTAGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACt > 1:4142245/1‑57 (MQ=12) tACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCaa > 1:5181823/1‑63 (MQ=255) tACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTc < 1:4635071/70‑1 (MQ=255) aCCTTTAGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTca < 1:1537705/70‑2 (MQ=18) aCCTTTAGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTca < 1:2125179/70‑2 (MQ=18) aCCTTTAGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTc > 1:207291/1‑69 (MQ=18) aCCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCg > 1:3167355/1‑70 (MQ=255) aCCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCg < 1:229860/70‑1 (MQ=255) ccTTTAGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTca < 1:2128302/69‑2 (MQ=18) cTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAg > 1:4586901/1‑71 (MQ=255) cTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAg > 1:1664479/1‑71 (MQ=255) ttAGATTGCAGGTAAGCGATGGCCGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGtt > 1:2825957/1‑71 (MQ=16) ttAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGATGTCGAACTGGTACTGAGCAACAGCTTCGAAGtt > 1:2920638/1‑71 (MQ=255) ttAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGtt > 1:1420880/1‑71 (MQ=255) ttAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGt > 1:640514/1‑70 (MQ=255) tAGATTGCAGGTAGGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGt < 1:676837/69‑1 (MQ=12) tAGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGtt < 1:4497202/70‑1 (MQ=16) tAGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATACTGAGCAACAACTTCAAAGtt < 1:3856330/70‑1 (MQ=16) tAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGtt < 1:4315465/70‑1 (MQ=255) aGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATa > 1:2225626/1‑48 (MQ=2) aGATTGCAGGTAAGCGATGGACGGGCGCAGGCCAAAGTCGAACTGATa > 1:2729144/1‑48 (MQ=2) gACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCt < 1:2782426/70‑1 (MQ=255) cTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCtgtg < 1:839806/71‑1 (MQ=255) | GCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACTGGTACTGAGCAACAGCTTCGAAGTTCTGTG > Exported/1439334‑1439472 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |